Objective:The antimicrobial activity of the ethanol extract of the Auklandia(Saussurea lappa)root plant was investigated to verify its medicinal use in the treatment of microbial infections.Methods:The antimicrobial a...Objective:The antimicrobial activity of the ethanol extract of the Auklandia(Saussurea lappa)root plant was investigated to verify its medicinal use in the treatment of microbial infections.Methods:The antimicrobial activity of the ethanol extract was tested against clinical isolates ofsome multidrug-resistant bacteria using the agar well diffusion method.Commercial antibioticswere used as positive reference standards to determine the sensitivity of the clinical isolates.Results:The extracts showed significant inhibitory activity against clinical isolates of methicillinresistantStaphylococcus aureus,Pseudomonas aeruginosa,Escherichia coli,Klebsiella pneumonia,Extended Spectrum Beta-Lactemase,Acinetobacter baumannii.The minimum inhibitory concentration values obtained using the agar dilution test ranged from 2.0μg/μL-12.0μg/μL.In the contrary the water extract showed no activity at all against the tested isolates.Furthermore,theresults obtained by examining anti-resistant activity of the plant ethanolic extract showed thatat higher concentration of the plant extract(12μg)all tested bacteria isolates were inhibited with variable inhibition zones similar to those obtained when we applied lower extract concentrationusing the well diffusion assay.Conclusion:The results demonstrated that the crude ethanolicextract of the Auklandia(Saussurea lappa)root plant has a wide spectrum of activity suggestingthat it may be useful in the treatment of infections caused by the above clinical isolates(humanpathogens).展开更多
Plants from the start are being used for the welfare of human and animals. About 25,000 biological active compounds are reported by different scientists. Plants itself are a complete treatment bioagent. People are sti...Plants from the start are being used for the welfare of human and animals. About 25,000 biological active compounds are reported by different scientists. Plants itself are a complete treatment bioagent. People are still using plants and their decoction for different diseases. Saussurea lappa Clarke is the member of family Compositae. This plant is famous due to its high medical importance. The plant is commonly named as Kuth root or costus and has wide use for anticancer, antiulcer, hepatoprotective, anti-viral, anticonvulsant, antiarthritic, activities. Biologically active substance of in this plant is lactone cynaropicrin, dehydrocostus, germacrene,?lappadilactone. This plant can be used to extract such bioactive compounds which can help the scientist to discover new and potential drugs. Due to such chemical composition and medicinal importance this review has been prepared for the awareness of the people to conserve their medicinal plants which can be used for potential drug discovery.展开更多
AIM:To study the constituents of Saussurea lappa and to find out bioactive compounds METHOD:Compounds were isolated by means of column chromatography,and the structures were identified by means of MS and NMR spectral ...AIM:To study the constituents of Saussurea lappa and to find out bioactive compounds METHOD:Compounds were isolated by means of column chromatography,and the structures were identified by means of MS and NMR spectral data RESULT:Nine compounds were isolated and identified as :dehydrocostus lactone (1),costunolide (2),β costic acid (3),reynosin (4),arbusculin A (5),4 allyl 2,6 dimethoxyphenyl glucoside (6),syringin(7),costunolid 15 β D glucopyranoside (8)and chlorogenic acid (9) CONCLUSION:Compounds 6 and 9 were isolated from Saussurea lappa for the first time and no compound showed bioactivity against PTP1B展开更多
目的应用高效液相色谱法检测云木香药材指纹图谱,采用多种化学计量学方法分析,以鉴别不同产地云木香药材.方法采用反相高效液相色谱法,乙腈-水为流动相梯度程序洗脱,流速1.0 m L/min,检测波长254 nm,检测云木香药材的HPLC图谱;结合相似...目的应用高效液相色谱法检测云木香药材指纹图谱,采用多种化学计量学方法分析,以鉴别不同产地云木香药材.方法采用反相高效液相色谱法,乙腈-水为流动相梯度程序洗脱,流速1.0 m L/min,检测波长254 nm,检测云木香药材的HPLC图谱;结合相似度计算、系统聚类分析、主成分分析等方法分析指纹图谱,对不同产地云木香进行鉴别.结果该方法可使云木香中各成分得到较好的分离;根据检测结果确定了18个共有指纹峰,建立了云木香药材指纹图谱的共有模式,15个不同来源的云木香有一定相似度;但不同产地云木香药材样品的指纹图谱通过化学计量学分析,发现存在较大差异.结论该HPLC指纹图谱重复性好,特异性高.HPLC指纹图谱联合化学计量学可鉴别不同产地云木香药材,为云木香药材质量控制提供依据,并为云木香药材资源合理开发利用提供参考.展开更多
基金Supported by the College of Medicine and Health Science,Sultan Qaboos University with the fund[Micro/Immu-Immu2013/Int/07]
文摘Objective:The antimicrobial activity of the ethanol extract of the Auklandia(Saussurea lappa)root plant was investigated to verify its medicinal use in the treatment of microbial infections.Methods:The antimicrobial activity of the ethanol extract was tested against clinical isolates ofsome multidrug-resistant bacteria using the agar well diffusion method.Commercial antibioticswere used as positive reference standards to determine the sensitivity of the clinical isolates.Results:The extracts showed significant inhibitory activity against clinical isolates of methicillinresistantStaphylococcus aureus,Pseudomonas aeruginosa,Escherichia coli,Klebsiella pneumonia,Extended Spectrum Beta-Lactemase,Acinetobacter baumannii.The minimum inhibitory concentration values obtained using the agar dilution test ranged from 2.0μg/μL-12.0μg/μL.In the contrary the water extract showed no activity at all against the tested isolates.Furthermore,theresults obtained by examining anti-resistant activity of the plant ethanolic extract showed thatat higher concentration of the plant extract(12μg)all tested bacteria isolates were inhibited with variable inhibition zones similar to those obtained when we applied lower extract concentrationusing the well diffusion assay.Conclusion:The results demonstrated that the crude ethanolicextract of the Auklandia(Saussurea lappa)root plant has a wide spectrum of activity suggestingthat it may be useful in the treatment of infections caused by the above clinical isolates(humanpathogens).
文摘Plants from the start are being used for the welfare of human and animals. About 25,000 biological active compounds are reported by different scientists. Plants itself are a complete treatment bioagent. People are still using plants and their decoction for different diseases. Saussurea lappa Clarke is the member of family Compositae. This plant is famous due to its high medical importance. The plant is commonly named as Kuth root or costus and has wide use for anticancer, antiulcer, hepatoprotective, anti-viral, anticonvulsant, antiarthritic, activities. Biologically active substance of in this plant is lactone cynaropicrin, dehydrocostus, germacrene,?lappadilactone. This plant can be used to extract such bioactive compounds which can help the scientist to discover new and potential drugs. Due to such chemical composition and medicinal importance this review has been prepared for the awareness of the people to conserve their medicinal plants which can be used for potential drug discovery.
文摘AIM:To study the constituents of Saussurea lappa and to find out bioactive compounds METHOD:Compounds were isolated by means of column chromatography,and the structures were identified by means of MS and NMR spectral data RESULT:Nine compounds were isolated and identified as :dehydrocostus lactone (1),costunolide (2),β costic acid (3),reynosin (4),arbusculin A (5),4 allyl 2,6 dimethoxyphenyl glucoside (6),syringin(7),costunolid 15 β D glucopyranoside (8)and chlorogenic acid (9) CONCLUSION:Compounds 6 and 9 were isolated from Saussurea lappa for the first time and no compound showed bioactivity against PTP1B
文摘目的应用高效液相色谱法检测云木香药材指纹图谱,采用多种化学计量学方法分析,以鉴别不同产地云木香药材.方法采用反相高效液相色谱法,乙腈-水为流动相梯度程序洗脱,流速1.0 m L/min,检测波长254 nm,检测云木香药材的HPLC图谱;结合相似度计算、系统聚类分析、主成分分析等方法分析指纹图谱,对不同产地云木香进行鉴别.结果该方法可使云木香中各成分得到较好的分离;根据检测结果确定了18个共有指纹峰,建立了云木香药材指纹图谱的共有模式,15个不同来源的云木香有一定相似度;但不同产地云木香药材样品的指纹图谱通过化学计量学分析,发现存在较大差异.结论该HPLC指纹图谱重复性好,特异性高.HPLC指纹图谱联合化学计量学可鉴别不同产地云木香药材,为云木香药材质量控制提供依据,并为云木香药材资源合理开发利用提供参考.
