目的预测疥螨猪变种转录组中过敏原蛋白,对Sar s 14.3过敏原蛋白进行生物特性分析、原核表达并建立间接ELISA诊断方法。方法利用转录组测序(RNA-Seq)及BLAST、ProtParam、I-TASSER和COMPARE等软件和数据库对疥螨猪变种转录组中的过敏原...目的预测疥螨猪变种转录组中过敏原蛋白,对Sar s 14.3过敏原蛋白进行生物特性分析、原核表达并建立间接ELISA诊断方法。方法利用转录组测序(RNA-Seq)及BLAST、ProtParam、I-TASSER和COMPARE等软件和数据库对疥螨猪变种转录组中的过敏原组分进行预测并对疥螨Sar s 14.3过敏原蛋白序列进行理化性质分析、结构分析、同源性分析和间接ELISA诊断方法的建立。结果在疥螨猪变种的18980个转录本中共预测出390条过敏原序列,且随机匹配可能性值(E-value)为0的序列有28条。Sar s 14.3过敏原蛋白的理论相对分子质量为38 kDa,蛋白较稳定,为亲水性蛋白;二级结构以无规卷曲和β-折叠为主,其氨基酸序列与疥螨其他变种主要过敏原组分14蛋白序列相似性不低于99.76%,而与尘螨属螨虫的相似性最高为73.08%,与现行的形态学分类系统一致。所建立的间接ELISA诊断方法的最佳包被抗原浓度为2μg/mL,血清最佳稀释倍数是1∶400。结论本试验成功预测了疥螨猪变种转录组中的过敏原序列并克隆、表达了疥螨猪变种Sar s 14.3蛋白,初步预测了其分子特征,并以该过敏原蛋白为基础成功建立了一种快速的间接ELISA检测方法,可用于疥螨病的初步诊断和流行病学调查。展开更多
Objectives: Scabies has a different epidemiological distribution among different communities worldwide due to different social factors, management approaches and healthcare policies. The present study came to address ...Objectives: Scabies has a different epidemiological distribution among different communities worldwide due to different social factors, management approaches and healthcare policies. The present study came to address outbreaks of scabies according to the social factors in west of Iran. Methods: In a cross-sectional study, using the census sampling method, all consecutive patients with the primary diagnosis of scabies based on clinical manifestations referred to healthcare center throughout the two great provinces of Hamadan and Kermanshah at western Iran between March 2006 and February 2010 were enrolled into the study. The baseline characteristics were collected from recorded files at the healthcare centers or by interviewing with the affected patients. Results: Among 3,625,966 subjects covered by the two studied provinces, 177 cases of scabies (170 cases in Kermanshah and 7 cases in Hamadan) were identified by medical staff at the healthcare centers according to clinical manifestations (85.3%) and laboratory microscopic assessments (14.7%). The highest and the lowest prevalence rates were specified to 2009 (58.2%) and 2008 (1.7%). Regarding gender distribution of disease, 53.1% of men and 46.9% of women suffered from scabies with no significant discrepancy. In respect to age distribution of disease, the highest rates of scabies were revealed in the ages ranged 17 to 30 years (31.6%), followed by younger than 17 years (30.5%). The prevalence of scabies was dependently associated with residency in rural areas, family history of scabies, lower educational level, household density, lower monthly income, low personal hygiene, the existence of livestock or rodents at home, seasonal conditions, and movement to contaminated areas. Conclusion: West region of Iran especially Kermanshah province faced with high prevalence and this high disease burden can be determined by some potential factors such as residency in rural areas, family history of scabies, lower educational level, household density, lower monthly income, low personal hygiene, the existence of livestock or rodents at home, seasonal conditions, and movement to contaminated areas.展开更多
文摘目的预测疥螨猪变种转录组中过敏原蛋白,对Sar s 14.3过敏原蛋白进行生物特性分析、原核表达并建立间接ELISA诊断方法。方法利用转录组测序(RNA-Seq)及BLAST、ProtParam、I-TASSER和COMPARE等软件和数据库对疥螨猪变种转录组中的过敏原组分进行预测并对疥螨Sar s 14.3过敏原蛋白序列进行理化性质分析、结构分析、同源性分析和间接ELISA诊断方法的建立。结果在疥螨猪变种的18980个转录本中共预测出390条过敏原序列,且随机匹配可能性值(E-value)为0的序列有28条。Sar s 14.3过敏原蛋白的理论相对分子质量为38 kDa,蛋白较稳定,为亲水性蛋白;二级结构以无规卷曲和β-折叠为主,其氨基酸序列与疥螨其他变种主要过敏原组分14蛋白序列相似性不低于99.76%,而与尘螨属螨虫的相似性最高为73.08%,与现行的形态学分类系统一致。所建立的间接ELISA诊断方法的最佳包被抗原浓度为2μg/mL,血清最佳稀释倍数是1∶400。结论本试验成功预测了疥螨猪变种转录组中的过敏原序列并克隆、表达了疥螨猪变种Sar s 14.3蛋白,初步预测了其分子特征,并以该过敏原蛋白为基础成功建立了一种快速的间接ELISA检测方法,可用于疥螨病的初步诊断和流行病学调查。
文摘Objectives: Scabies has a different epidemiological distribution among different communities worldwide due to different social factors, management approaches and healthcare policies. The present study came to address outbreaks of scabies according to the social factors in west of Iran. Methods: In a cross-sectional study, using the census sampling method, all consecutive patients with the primary diagnosis of scabies based on clinical manifestations referred to healthcare center throughout the two great provinces of Hamadan and Kermanshah at western Iran between March 2006 and February 2010 were enrolled into the study. The baseline characteristics were collected from recorded files at the healthcare centers or by interviewing with the affected patients. Results: Among 3,625,966 subjects covered by the two studied provinces, 177 cases of scabies (170 cases in Kermanshah and 7 cases in Hamadan) were identified by medical staff at the healthcare centers according to clinical manifestations (85.3%) and laboratory microscopic assessments (14.7%). The highest and the lowest prevalence rates were specified to 2009 (58.2%) and 2008 (1.7%). Regarding gender distribution of disease, 53.1% of men and 46.9% of women suffered from scabies with no significant discrepancy. In respect to age distribution of disease, the highest rates of scabies were revealed in the ages ranged 17 to 30 years (31.6%), followed by younger than 17 years (30.5%). The prevalence of scabies was dependently associated with residency in rural areas, family history of scabies, lower educational level, household density, lower monthly income, low personal hygiene, the existence of livestock or rodents at home, seasonal conditions, and movement to contaminated areas. Conclusion: West region of Iran especially Kermanshah province faced with high prevalence and this high disease burden can be determined by some potential factors such as residency in rural areas, family history of scabies, lower educational level, household density, lower monthly income, low personal hygiene, the existence of livestock or rodents at home, seasonal conditions, and movement to contaminated areas.
