The translation of current tissue engineering approaches to clinical application is somehow limited by the use of scaffolding materials.Recently a number of in vitro scaffold-free three-dimensional culture techniques ...The translation of current tissue engineering approaches to clinical application is somehow limited by the use of scaffolding materials.Recently a number of in vitro scaffold-free three-dimensional culture techniques have been developed.These techniques realize the assembly of tissue-like structures including but not limited to spheroids,blood vessels and cartilage.In particular,cells can now self-assemble to form planar tissue-like structures at the interface of an aqueous-two-phase system(ATPS).The unique advantage of this technique is that without a solid substrate,planar tissue-like structures can now be assembled rapidly with very simple procedures.This technique can potentially be very useful for tissue engineering in eye because of its ability to direct cells to form monolayer.In this talk,we will introduce what ATPS is and its current applications in biomedical research.We will then present an approach to assemble cell sheets in ATPS using both primary cells isolated from porcine eyes and other cell lines.The physiological relevance of these eye-related cell sheets as well as their potentials in ophthalmic research and applications will be discussed.展开更多
目的探讨根尖乳头干细胞(stem cells from the apical papilla,SCAP)在无支架三维培养条件下的生物学行为。方法用显微镜和HR染色观察SCAP球的大体形态,组织学结构;用免疫组化法检测SCAP球中骨涎蛋白(bone sialoprotein,BSP)和血管性血...目的探讨根尖乳头干细胞(stem cells from the apical papilla,SCAP)在无支架三维培养条件下的生物学行为。方法用显微镜和HR染色观察SCAP球的大体形态,组织学结构;用免疫组化法检测SCAP球中骨涎蛋白(bone sialoprotein,BSP)和血管性血友病因子(von Willebrand factor,vWF)的表达。结果细胞球的大小对球体内部细胞的生长有影响,SCAP球具有向成骨和向内皮分化的潜能。结论无支架三维培养是一种可行的SCAP培养方法。展开更多
文摘The translation of current tissue engineering approaches to clinical application is somehow limited by the use of scaffolding materials.Recently a number of in vitro scaffold-free three-dimensional culture techniques have been developed.These techniques realize the assembly of tissue-like structures including but not limited to spheroids,blood vessels and cartilage.In particular,cells can now self-assemble to form planar tissue-like structures at the interface of an aqueous-two-phase system(ATPS).The unique advantage of this technique is that without a solid substrate,planar tissue-like structures can now be assembled rapidly with very simple procedures.This technique can potentially be very useful for tissue engineering in eye because of its ability to direct cells to form monolayer.In this talk,we will introduce what ATPS is and its current applications in biomedical research.We will then present an approach to assemble cell sheets in ATPS using both primary cells isolated from porcine eyes and other cell lines.The physiological relevance of these eye-related cell sheets as well as their potentials in ophthalmic research and applications will be discussed.
文摘目的探讨根尖乳头干细胞(stem cells from the apical papilla,SCAP)在无支架三维培养条件下的生物学行为。方法用显微镜和HR染色观察SCAP球的大体形态,组织学结构;用免疫组化法检测SCAP球中骨涎蛋白(bone sialoprotein,BSP)和血管性血友病因子(von Willebrand factor,vWF)的表达。结果细胞球的大小对球体内部细胞的生长有影响,SCAP球具有向成骨和向内皮分化的潜能。结论无支架三维培养是一种可行的SCAP培养方法。
