The mechanism for interactions of the 6-benzyl-amino-purine and its Co2+, Ni2+ complexes with DNA was studied by UV and fluorescence spectra using ethidium bromide (EB) as probe molecule. When pH=7.4, the action of 6-...The mechanism for interactions of the 6-benzyl-amino-purine and its Co2+, Ni2+ complexes with DNA was studied by UV and fluorescence spectra using ethidium bromide (EB) as probe molecule. When pH=7.4, the action of 6-benzyl-amino-purine-Co2+ and DNA was electrostatic effect and certain kind of intercalation action; when pH=9.86, the action of the two was electrostatic effect; when pH=2.85 , the action of 6-benzyl-amino-purine-Ni2+ and DNA was both electrostatic and intercalation action. But when pH=7.4, 6-benzyl-amino-purine hardly influenced the intercalation action of EB and DNA, the action of 6-benzyl-amino-purine and DNA was electrostatic action.展开更多
以乙酸镍和2,2′-联吡啶配合物为模板、4-乙烯吡啶为功能单体、乙二醇二甲基丙烯酸酯为交联剂,合成了具有类似金属螯合抗体结合位点的金属配合物印迹聚合物,系统研究了金属离子对模板聚合物选择性结合2,2′-联吡啶的调节作用。结果表明...以乙酸镍和2,2′-联吡啶配合物为模板、4-乙烯吡啶为功能单体、乙二醇二甲基丙烯酸酯为交联剂,合成了具有类似金属螯合抗体结合位点的金属配合物印迹聚合物,系统研究了金属离子对模板聚合物选择性结合2,2′-联吡啶的调节作用。结果表明:印迹聚合物对N i(Ⅱ)-2,2′-联吡啶配合物有选择性识别能力。采用Scatchard模型评价分子印迹聚合物的结合特性,得到高亲和力结合位点的平衡离解常数Kd1=0.082 mm o l/L,表观最大结合量Qm ax1=82.2μm o l/g,低亲和力结合位点的Kd2=0.400 mm o l/L,Qm ax2=91.3μm o l/g。展开更多
Using nickel（II） acetate.-2,2＇-dipyridyl complex as template and N-vinyl-2-pyrrolidone （NVP） as coordinate functional monomer,.a new kind of metal-compiexing template molecularly impnnted polymer （MIP） was prep...Using nickel（II） acetate.-2,2＇-dipyridyl complex as template and N-vinyl-2-pyrrolidone （NVP） as coordinate functional monomer,.a new kind of metal-compiexing template molecularly impnnted polymer （MIP） was prepared..The results of equilibri.um binding experiments in. aqueous solution showed that the MIP had higher＇binding capacity for nickel（ II ）-2,2＇-dipyridyl than the non-imprinted polymer with the same chemical composition. Theinfluences of metal ions and pHof solution on the recognition performance of MIP were investigated. The bindingcharacteristics of MIP were evaluated by the Scatchard analysis with one-site and two-site binding equations, respectively. The results on substrate selectivity of imprinted polymer revealed that MIP had better binding affinityfor template among thetested compounds.展开更多
Fluorescence of Ce 3+ (aq) is very strong and thoroughly quenched when Ce 3+ forms complexes with ligands containing carboxylate or phosphate groups. This property is employed to develop a new simple method t...Fluorescence of Ce 3+ (aq) is very strong and thoroughly quenched when Ce 3+ forms complexes with ligands containing carboxylate or phosphate groups. This property is employed to develop a new simple method to determine formation constants of Ce 3+ complex system. Fluorescence intensity is used to determine concentration of Ce 3+ (aq) in complex solution, as it is proportional to the concentration of Ce 3+ (aq). In this paper, two examples, Ce 3+ malonic acid and Ce 3+ AMP(adenosine 5′ monophosphate), of fluorescence titration were made and association constants and association numbers of the two systems were deduced for the titration with Scatchard plot. The results are similar to those obtained by others. It is suggested that this Ce 3+ method is a promising fluorescence probe, as it is not only simple but also superior to other lanthanide fluorescence probe in being able to study a large amount of organic ligands and biomolecules.展开更多
In this study the complex Mg（Sal2trien） was synthesized for the first time, the binding mode of which with CT DNA was studied by the methods of UV spectra, fluorescence spectra, viscosity and CV （cyclic voltammetry...In this study the complex Mg（Sal2trien） was synthesized for the first time, the binding mode of which with CT DNA was studied by the methods of UV spectra, fluorescence spectra, viscosity and CV （cyclic voltammetry）. It was found that after the com- plex acted with CT DNA, the Abs of UV spectra rose obviously; the fluorescence intensity of EB-DNA was almost not changed; viscosity decreased. Determina- tion of cyclic voltammetry showed that DNA made the MgL’s formal potential negatively shift. Scatchard plot showed that the addition of the binding mode of the complex to EB was uncompetitive inhibition com- pared with EB to DNA. So the binding mode of the complex with CT DNA was stable-electricity binding. Then the interaction of the complex with pBR322 was studied by the method of gel electrophoresis. The result showed that the complex could cleave pBR322 DNA effectively.展开更多
文摘The mechanism for interactions of the 6-benzyl-amino-purine and its Co2+, Ni2+ complexes with DNA was studied by UV and fluorescence spectra using ethidium bromide (EB) as probe molecule. When pH=7.4, the action of 6-benzyl-amino-purine-Co2+ and DNA was electrostatic effect and certain kind of intercalation action; when pH=9.86, the action of the two was electrostatic effect; when pH=2.85 , the action of 6-benzyl-amino-purine-Ni2+ and DNA was both electrostatic and intercalation action. But when pH=7.4, 6-benzyl-amino-purine hardly influenced the intercalation action of EB and DNA, the action of 6-benzyl-amino-purine and DNA was electrostatic action.
文摘以乙酸镍和2,2′-联吡啶配合物为模板、4-乙烯吡啶为功能单体、乙二醇二甲基丙烯酸酯为交联剂,合成了具有类似金属螯合抗体结合位点的金属配合物印迹聚合物,系统研究了金属离子对模板聚合物选择性结合2,2′-联吡啶的调节作用。结果表明:印迹聚合物对N i(Ⅱ)-2,2′-联吡啶配合物有选择性识别能力。采用Scatchard模型评价分子印迹聚合物的结合特性,得到高亲和力结合位点的平衡离解常数Kd1=0.082 mm o l/L,表观最大结合量Qm ax1=82.2μm o l/g,低亲和力结合位点的Kd2=0.400 mm o l/L,Qm ax2=91.3μm o l/g。
基金Supported by the Special Funds for Major State Basic Research Program of China(973 Project,No.2003CB615705).
文摘Using nickel（II） acetate.-2,2＇-dipyridyl complex as template and N-vinyl-2-pyrrolidone （NVP） as coordinate functional monomer,.a new kind of metal-compiexing template molecularly impnnted polymer （MIP） was prepared..The results of equilibri.um binding experiments in. aqueous solution showed that the MIP had higher＇binding capacity for nickel（ II ）-2,2＇-dipyridyl than the non-imprinted polymer with the same chemical composition. Theinfluences of metal ions and pHof solution on the recognition performance of MIP were investigated. The bindingcharacteristics of MIP were evaluated by the Scatchard analysis with one-site and two-site binding equations, respectively. The results on substrate selectivity of imprinted polymer revealed that MIP had better binding affinityfor template among thetested compounds.
文摘Fluorescence of Ce 3+ (aq) is very strong and thoroughly quenched when Ce 3+ forms complexes with ligands containing carboxylate or phosphate groups. This property is employed to develop a new simple method to determine formation constants of Ce 3+ complex system. Fluorescence intensity is used to determine concentration of Ce 3+ (aq) in complex solution, as it is proportional to the concentration of Ce 3+ (aq). In this paper, two examples, Ce 3+ malonic acid and Ce 3+ AMP(adenosine 5′ monophosphate), of fluorescence titration were made and association constants and association numbers of the two systems were deduced for the titration with Scatchard plot. The results are similar to those obtained by others. It is suggested that this Ce 3+ method is a promising fluorescence probe, as it is not only simple but also superior to other lanthanide fluorescence probe in being able to study a large amount of organic ligands and biomolecules.
基金This work was supported by the National Natural Science Foundation of China(Grant No.30470408)the Natural Science Foundation of Shanxi Province(Grant No.20041032).
文摘In this study the complex Mg（Sal2trien） was synthesized for the first time, the binding mode of which with CT DNA was studied by the methods of UV spectra, fluorescence spectra, viscosity and CV （cyclic voltammetry）. It was found that after the com- plex acted with CT DNA, the Abs of UV spectra rose obviously; the fluorescence intensity of EB-DNA was almost not changed; viscosity decreased. Determina- tion of cyclic voltammetry showed that DNA made the MgL’s formal potential negatively shift. Scatchard plot showed that the addition of the binding mode of the complex to EB was uncompetitive inhibition com- pared with EB to DNA. So the binding mode of the complex with CT DNA was stable-electricity binding. Then the interaction of the complex with pBR322 was studied by the method of gel electrophoresis. The result showed that the complex could cleave pBR322 DNA effectively.
