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Effects of Chuanxiongqin hydrochloride on increasing the fluidity of brain cell membrane and scavenging free radicals in model rats with ischemia/reperfusion injury 被引量:1
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作者 Chenxu Li Institute of Brain Sciences Department of Physiology, Medical College, Datong University, Datong 037008, Shanxi Province, China 《Neural Regeneration Research》 SCIE CAS CSCD 2006年第8期721-724,共4页
BACKGROUND: The fluidity of cell membrane can be affected by various factors. Many experiments have confirmed that the ischemia/reperfusion of organic tissue can increase the contents of free radicals, which lead to h... BACKGROUND: The fluidity of cell membrane can be affected by various factors. Many experiments have confirmed that the ischemia/reperfusion of organic tissue can increase the contents of free radicals, which lead to high rigidity and low fluidity of cell membrane, and the conditions can be changed by Chuanxiongqin. OBJECTIVE: To observe the effect and mechanism of Chuanxiongqin hydrochloride on the fluidity of brain cell membrane in rat models of ischemia/reperfusion. DESIGN: A completely randomized controlled animal trial. SETTINGS: Institute of Brain Sciences; Department of Physiology, Medical College, Datong University. MATERIALS: Twenty male grade Ⅰ Wistar rats of 170-220 g were randomly divided into model group (n =10) and control group (n =10). Chuanxiongqin hydrochloride (molecular mass was 172.2) was purchased from the National Institute for the Control of Pharmaceutical and Biological Products (batch number: 0817-9803); Spin labelers: 5-doxyl-stearlic acid methylester (5DS), 16-doxyl-stearlic acid methylester (16DS), xanthine, xanthine oxidase (XOD) and 5,5-dimeth-1-pyrroline- N-oxide (DMPO) from Sigma Company; Bruker ESP 300 electron paramagnetic resonance (EPR) spectrometer by Bruker Company (Germany). METHODS: The experiments were carried out in the State Key Laboratory of Natural and Biomimetic Drugs, Peking University from June 2001 to July 2002. In the model group, rats were made into models of cerebral ischemia by 30-minute ligation and 2-hour reperfusion of common carotid arteries; The rats in the control group were not made into models. The order parameter (S) and rotational correlation time (τc) were detected with the ESR spectrometer by means of spin labeling. The greater the S and τc, the smaller the fluidity. Meanwhile, the clearance rate of free radicals was detected with ESR spin trapping. The measurement data were compared using the t test. MAIN OUTCOME MEASURES: The S, τc and clearance rates of O2 · and OH· free radicals were compared between the model group and control group. RESULTS: The S and τc in the model group [0.738 4±0.003 5; (8.472±0.027)×10-10 s/circle] were obviously different from those in the control group [0.683 9±0.008 3; (7.945±0.082)×10-10 s/circle, t =5.731, 5.918, P < 0.05], which suggested that ischemia/reperfusion injury decreased the fluidity of brain cell membrane. After adding Chuanxiongqin hydrochloride, there were no obvious differences between the model group [0.688 5±0.030 5; (7.886±0.341)×10-10 s/circle] and control group (P > 0.05), indicating that Chuanxiongqin hydrochloride could recover the fluidity of brain cell membrane after ischemia/reperfusion injury close to the level in the normal control group. Chuanxiongqin hydrochloride could directly scavenge the O2 · and OH· free radicals, and the maximal clearance rates were 83.92% and 44.99% respectively. CONCLUSION: Chuanxiongqin hydrochloride increases the fluidity of membrane of ischemia-injured brain cell by scavenging both O2 ·and OH· free radicals. 展开更多
关键词 Effects of Chuanxiongqin hydrochloride on increasing the fluidity of brain cell membrane and scavenging free radicals in model rats with ischemia/reperfusion injury cell free
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Extraction Storage and Qualitative Analysis of Free Radicals Scavenging Substances from Sweet Potato Leaves 被引量:9
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作者 王友升 董银卯 +1 位作者 宋彦 黑维俭 《Agricultural Science & Technology》 CAS 2008年第2期1-5,9,共6页
[ Objective ] The research aimed to provide reference for the application of extracts from sweet potato leaves in anti-aging cosmetics. [ Method ] The extraction and storage conditions for free radicals scavenging sub... [ Objective ] The research aimed to provide reference for the application of extracts from sweet potato leaves in anti-aging cosmetics. [ Method ] The extraction and storage conditions for free radicals scavenging substances from sweet potato leaves were optimized by orthogonal test and the bioactive components in extracts were investigated by correlation analysis. [ Result] Sweet potato leaves contain the bioactive substances scavenging DPPH free radical and hydroxyl free radical. Extracting solvent species is the most important factor that influencing extraction yield. The optimal extraction and storage conditions are as following: water as solvent, pH 8.0 of extracting liquid, storage at 25 ℃. There is a good positive linear relationship between the content of total phenols in sweet potato leaves and corresponding scavenging rates against both the DPPH free radical and hydroxyl free radical. For the content of total flavones in sweet potato leaves, just a correlation with scavenging rate against hydroxyl free radical shown in test. [ Conclusion] The phenols in ex- tracts could effectively scavenge both the DPPH free radical and hydroxyl free radical, whereas the flavones in extracts can only function on the hydroxyl free radical. 展开更多
关键词 Sweet potato leaves free radicals scavenging Total phenol Total flavones
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Component Analysis and Free Radicals Scavenging Activity of Physalis alkekengi L. Polysaccharide 被引量:19
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作者 CHENG Ying-kun LI Lei MENG Zhao-kun HOU A-li WU Yu-jie TENG Li-rong 《Chemical Research in Chinese Universities》 SCIE CAS CSCD 2008年第2期167-170,共4页
A crude polysaccharide was extracted from Physalis alkekengi L. fruit. HPLC was used for the component analysis of the polysaccharide. The results indicate that Physalis alkekengi L. polysaccharide(PAP) was composed... A crude polysaccharide was extracted from Physalis alkekengi L. fruit. HPLC was used for the component analysis of the polysaccharide. The results indicate that Physalis alkekengi L. polysaccharide(PAP) was composed of rhamnose, xylose, arabinose, galactose, and glucose. Free radicals scavenging activity of PAP was studied through 3 free radicals scavenging tests. PAP exhibited high scavenging effects on OH and DPPH radicals, and both the scavenging rates were about 80%. The scavenging rate of O2^- radical was about 22%. 展开更多
关键词 PHYSALIS POLYSACCHARIDE Component analysis free radicals scavenging activity
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Butein imparts free radical scavenging, anti-oxidative and proapoptotic properties in the flower extracts of Butea monosperma 被引量:3
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作者 ANURADHA SEHRAWAT VIJAY KUMAR 《BIOCELL》 SCIE 2012年第2期63-71,共9页
The flower of Butea monosperma(Lam.)(Fabaceae)has been used in traditional Indian medicine in the treatment of many ailments including liver disorders.To understand the pharmacological basis of its beneficial effects,... The flower of Butea monosperma(Lam.)(Fabaceae)has been used in traditional Indian medicine in the treatment of many ailments including liver disorders.To understand the pharmacological basis of its beneficial effects,the extracts of dried flowers in water,methanol,butanol,ethyl acetate and acetone were evaluated for free radical scavenging and pro-apoptotic activities in cell cultures(human hepatoma Huh-7 cell line and immortalized AML-12 mouse hepatocytes).Butrin and butein-the active constituents of flower extracts-were used as reference molecules.The levels of cell injury markers like lactate dehydrogenase,glutathione and lipid peroxidation and primary antioxidant enzymes glutathione S-transferase and catalase were also measured.The aqueous and butanolic extracts exhibited better 2,2-diphenyl-1-picrylhydrazyl scavenging and cytotoxic activities in hepatoma cells than in immortalized hepatocytes.Interestingly,butein inhibited 2,2-diphenyl-1-picrylhydrazyl radical better than butrin.The aqueous and butanolic extracts were further investigated for hepatoprotection against carbon tertrachloride-induced biochemical changes and cell death.Both extracts,just as butrin and butein,significantly reversed the cellular glutathione levels and lipid peroxidation,and glutathione–S-transferase activity.Lactate dehydrogenase leakage and cell death were also prevented.However,only butein revived the catalase activity.Thus,the butein content of Butea monosperma flower extracts is important for free radical scavenging activity,apoptotic cell death and protection against oxidative injury in hepatic cells. 展开更多
关键词 butrin CYTOTOXICITY HEPATOPROTECTION methanol extract free radical scavenging
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Study on the Free Radical Scavenging Activity of Sea Cucumber (Paracaudina chinens var.) Gelatin Hydrolysate 被引量:1
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作者 ZENG Mingyong XIAO Feng ZHAO Yuanhui LIU Zunying LI Bafang DONG Shiyuan 《Journal of Ocean University of China》 SCIE CAS 2007年第3期255-258,共4页
Gelatin from the sea cucumber(Paracaudina chinens var.) was hydrolyzed by bromelain and the hydrolysate was found to have a high free radical scavenging activity. The hydrolysate was fractionated through an ultrafiltr... Gelatin from the sea cucumber(Paracaudina chinens var.) was hydrolyzed by bromelain and the hydrolysate was found to have a high free radical scavenging activity. The hydrolysate was fractionated through an ultrafiltration membrane with 5 kDa molecular weight cutoff(MWCO). The portion(less than 5 kDa) was further separated by Sephadex G-25. The active peak was col-lected and assayed for free radical scavenging activity. The scavenging rates for superoxide anion radicals(O2·-) and hydroxyl radi-cals(·OH) of the fraction with the highest activity were 29.02% and 75.41%,respectively. A rabbit liver mitochondrial free radical damage model was adopted to study the free radical scavenging activity of the fraction. The results showed that the sea cucumber gelatin hydrolysate can prevent the damage of rabbit liver and mitochondria. 展开更多
关键词 Paracaudina chinens var. gelatin hydrolysate free radical scavenging activity MITOCHONDRIA
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Evaluation of lipid peroxidation inhibition and free radical scavenging abilities of 5,6,7-trimethoxy dihydroflavonols
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作者 Kc Xin Huang Yu Bing Feng +10 位作者 Wei Yao Lei Xiang Yang Feng Wang Hai Bo Li Su Zeng Jing Xu Gong Ming Hui Hu Yu Zhao Xiu Mei Wu Xiao Kun Li Jia Qu 《Chinese Chemical Letters》 SCIE CAS CSCD 2009年第10期1187-1190,共4页
Four naturally rare 5,6,7-trimethoxy-2,3-cis-dihydroflavonols (3-6) and two 5,6,7-trimethoxy-2,3-trans-dihydroflavonols (7-8) were designed and synthesized. Their antioxidative properties were evaluated by way of ... Four naturally rare 5,6,7-trimethoxy-2,3-cis-dihydroflavonols (3-6) and two 5,6,7-trimethoxy-2,3-trans-dihydroflavonols (7-8) were designed and synthesized. Their antioxidative properties were evaluated by way of examining their scavenging capacities towards DPPH and O2^*- free radicals, as well as by measuring their inhibitory ability against LPO. Both the 2,3-trans and the 2,3- cis conformers exhibited certain quenching abilities to DPPH and O2^*- radicals, while most of the synthetic dihydroflavonols demonstrated remarkable inhibition to LPO. 展开更多
关键词 5 6 7-Trimethoxy-2 3-dihydroflavonols cis-Dihydroflavonols ANTIOXIDANT Lipid peroxidation free radical scavenger
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Why Static O-H Bond Parameters Cannot Characterize the Free Radical Scavenging Activity of Phenolic Antioxidants: ab initio Study
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作者 Hong Yu ZHANG You Min SUN De Zhan CHEN 《Chinese Chemical Letters》 SCIE CAS CSCD 2001年第1期75-78,共4页
The static O-H bond parameters including O-H bond length, O-H charge difference, O-H Mulliken population and O-H bond stretching force constant (k) for 17 phenols were calculated by ab initio method HF/6-31G**. In com... The static O-H bond parameters including O-H bond length, O-H charge difference, O-H Mulliken population and O-H bond stretching force constant (k) for 17 phenols were calculated by ab initio method HF/6-31G**. In combination with the O-H bond dissociation enthalpies (BDE) of the phenols determined by experiment, it was found that there were poor correlationships between the static O-H bond parameters and O-H BDE. Considering the good correlationship bt tween O-H BDE and logarithm of free radical scavenging rate constant for phenolic antioxidant, it is reasonable to believe that the ineffectiveness of static O-H bond parameters in characterizing antioxidant activity arises from the fact that they cannot measure the O-H BDE. 展开更多
关键词 ANTIOXIDANTS free radical scavenging effect O-H bond dissociation enthalpy O-H bond length O-H bond stretching force constant O-H charge difference O-H Mulliken population QSAR
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Free Radical-Scavenging Properties and Antioxidant Activity of Fractions from Cranberry Products
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作者 Stéphane Caillet Guillaume Lorenzo +2 位作者 Jacinthe Coté Jean-Francois Sylvain Monique Lacroix 《Food and Nutrition Sciences》 2012年第3期337-347,共11页
Lipid peroxidation inhibition capacity and antiradical activity were evaluated in HPLC fractions of different polarity obtained from two cranberry juices and three extracts isolated from frozen cranberries and pomace ... Lipid peroxidation inhibition capacity and antiradical activity were evaluated in HPLC fractions of different polarity obtained from two cranberry juices and three extracts isolated from frozen cranberries and pomace containing antho-cyanins, water-soluble and apolar phenolic compounds, respectively. Compounds with close polarities were collected to obtain between three and four fractions from each juice or extract. The cranberry phenols are good free radi-cal-scavengers, but they were less efficient at inhibiting the lipid peroxidation. Of all the samples tested, the intermediate polarity fraction of extract rich in apolar phenolic compounds of fruit presented the highest antiradical activity while the most hydrophobic fractions of the anthocyanin-rich extract from fruit and pomace appeared to be the most efficient at inhibiting the lipid peroxidation. The antioxidant or pro-oxidant activity of fractions increased with the con-centration. The phenol polarity and the technological process to manufacture cranberry juice can influence the antioxidant and antiradical activities of fractions. 展开更多
关键词 HPLC Fractions Cranberry Juices Phenolic Extracts free Radical scavenging Capacity Antioxidant Activity
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Application of Theories of Free Radicals and Electron Spin Resonance for Research of Traditional Chinese Medicine
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作者 段绍瑾 《Journal of Traditional Chinese Medicine》 SCIE CAS CSCD 2000年第1期51-58,共1页
The present paper reviews new findings in redoxproperties of the active constituent of Chinese herbalmedicine(CHM),a kind of CHM or a compoundprescription as an antioxidant.Firstly,we have studiedtheir antioxidant and... The present paper reviews new findings in redoxproperties of the active constituent of Chinese herbalmedicine(CHM),a kind of CHM or a compoundprescription as an antioxidant.Firstly,we have studiedtheir antioxidant and prooxidant actions with electronspin resonance(ESR).The results show that the activecomponents from over 10 kinds of CHM are able toscavenge the oxygen free radicals but propyl gallate 展开更多
关键词 Medicine Chinese Traditional ANTIOXIDANTS Drugs Chinese Herbal Electron Spin Resonance Spectroscopy free Radical Scavengers Humans Neurosecretory Systems Oxidative Stress
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Conformationally regulated“nanozyme-like”cerium oxide with multiple free radical scavenging activities for osteoimmunology modulation and vascularized osseointegration 被引量:1
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作者 Shusen Bao Dongmei Yu +8 位作者 Zhen Tang Hao Wu Hao Zhang Ning Wang Yichao Liu Hai Huang Chaozong Liu Xiaokang Li Zheng Guo 《Bioactive Materials》 SCIE CSCD 2024年第4期64-79,共16页
Given post-operative aseptic loosening in orthopedic disease treatment,osteointegration occurs at the bone-implant interface as a holistic process,including immunoregulation(e.g.,macrophage polarization),angiogenesis ... Given post-operative aseptic loosening in orthopedic disease treatment,osteointegration occurs at the bone-implant interface as a holistic process,including immunoregulation(e.g.,macrophage polarization),angiogenesis and osteogenesis in sequence.In order to achieve early rapid and satisfactory osseointegration,different nano-shaped(nanocone,nanopolyhedron and nanoflower abbr.NC,NP&NF)cerium oxide(CeO_(2-x))coatings,endowed with“nanozyme-like”activities for multiple free radical elimination and osteoimmunology regulation,were hydrothermally synthesized on titanium alloy(TC4).In vitro cell experiments showed that nano-CeO_(2-x) coated TC4 not only induced polarization of RAW264.7 cells toward M2 phenotype,but also promoted angiogenesis and vascularization of endothelial cells along with differentiation and mineralization of osteogenic precursor cells.Improvements in M2-polarized macrophage,angiogenesis,and bone regeneration were further confirmed in a rat femoral condyle model.Among the above three nano-morphologies,NF exhibited the best osseoinetegration.RNA sequencing and mechanism exploration suggested that the inhibition of PI3K-AKT signaling pathway was essential for immunomodulatory capacity of NF.In conclusion,it provided promising insights into the immunomodulatory exploitation of orthopedic implants. 展开更多
关键词 Nanozyme-like”cerium oxide Durable redox activation free radical scavenging Osteoimmunology modulation Vascularized osseointegration
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Studies on Biologically Active Principles of Huangqi,Root of Astragalus membranaceous,Isolation and Detection of Constituents Scavenging Superoxide Anion
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作者 刘星堦 江明华 +4 位作者 喻正坤 郑基蒙 龚志铭 张静华 戴瑞鸿 《Journal of Chinese Pharmaceutical Sciences》 CAS 1993年第1期80-84,共5页
本文使用黄嘌呤-黄嘌呤氧化酶—鲁米诺化学发光体系和化学发光检测法研究了黄芪中各成分的清除超氧阴离子自由基的能力,以药物抑制发光强度50%的浓度(IC_(50))为指标。经研究证明,黄芪总皂甙部分(N)具有较强的活性,IC_(50)为185μg/ml,... 本文使用黄嘌呤-黄嘌呤氧化酶—鲁米诺化学发光体系和化学发光检测法研究了黄芪中各成分的清除超氧阴离子自由基的能力,以药物抑制发光强度50%的浓度(IC_(50))为指标。经研究证明,黄芪总皂甙部分(N)具有较强的活性,IC_(50)为185μg/ml,再经进一步导向分离并鉴定证明,黄芪甙Ⅲ,Ⅳ和Ⅵ的 IC)_(50)分别为80、50和11μg/ml,从而证明黄芪的抗心力衰竭的有效成分可能为黄芪总皂甙部分。 展开更多
关键词 Astragalus membranaceous Astragalosides and Oxygen free radical scavenger Superoxide anion Chemiluminescence
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Antioxidant phenanthrenes and lignans from Dendrobium nobile 被引量:6
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作者 张雪 续洁琨 +2 位作者 王乃利 栗原博 姚新生 《Journal of Chinese Pharmaceutical Sciences》 CAS 2008年第4期314-318,共5页
To study the antioxidant constituents from the stems of Dendrobium nobile, and to discuss theft structure-activity relationship. Compounds were isolated from a 60% ethanolic extract by various chromatographic techniqu... To study the antioxidant constituents from the stems of Dendrobium nobile, and to discuss theft structure-activity relationship. Compounds were isolated from a 60% ethanolic extract by various chromatographic techniques and were identified by spectral analysis. The antioxidant activities of compounds were evaluated by DPPH free radical scavenging assay. Five phenanthrenes and four lignans were obtained from the active fractions ofD. nobile. Their structures were identified as fimbriatone (1), confusarin (2), flavanthrinin (3), 2,5-dihydroxy-4,9-dimethoxyphenanthrene (4), 3,7-dihydroxy-2,4-dimethoxyphenanthrene (5), syringaresinol (6), pinoresinol (7), medioresinol (8) and lirioresinol-A (9), respectively. Compounds 2 and 6 exhibited more potent DPPH scavenging activities than vitamin C. All the above compounds were reported from this plant for the first time, and compounds 3, 4 and 9 were reported for the first time from the genus of Dendrobiurn. For all phenanthrenes and lignans, an electron-donating methoxyl group in the ortho position to the phenolic hydroxyl group exhibits enhanced antioxidant activities. 展开更多
关键词 Dendrobium nobile PHENANTHRENES Lignans Antioxidant activity DPPH free radical scavenging assay Structureactivity relationship
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Free radical scavenging activity of Eagle tea and their flavonoids 被引量:4
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作者 Qiong Meng Zhengming Qian +4 位作者 Xiaoxiang Li Deqiang Li Weihua Huang Jing Zhao Shaoping Li 《Acta Pharmaceutica Sinica B》 SCIE CAS 2012年第3期246-249,共4页
In this study,an online HPLC-DAD-MS coupled with 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)diammonium salt(A BTS)assay was employed for evaluating free radical scavenging activity of Eagle tea and their ... In this study,an online HPLC-DAD-MS coupled with 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)diammonium salt(A BTS)assay was employed for evaluating free radical scavenging activity of Eagle tea and their active components.Twenty-three chromatographic peaks were detected,and nineteen components had free radical scavenging activity.Among them,eight compounds were identified as flavonoids(hyperin,isoquercitrin,quercitrin,quercetin,kaempferol),catechins,chlorogenic acid and epicatechin based on MS data and standard chromatographic characters. 展开更多
关键词 ABTS Eagle tea FLAVONOIDS free radical scavenging HPLC
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N-acetylcysteine attenuates alcohol-induced oxidative stress in the rat 被引量:7
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作者 Resat Ozaras Veysel Tahan +3 位作者 Seval Aydin Hafize Uzun Safiye Kaya Hakan Senturk 《World Journal of Gastroenterology》 SCIE CAS CSCD 2003年第1期125-128,共4页
AIM: There is increasing evidence that alcohol-induced liverdamage may be associated with increased oxidative stress.We aimed to investigate free-radical scavenger effect of n-acetylcysteine in rats intragastrically f... AIM: There is increasing evidence that alcohol-induced liverdamage may be associated with increased oxidative stress.We aimed to investigate free-radical scavenger effect of n-acetylcysteine in rats intragastrically fed with ethanol.METHODS: Twenty-four rats divided into three groups werefed with ethanol (6 g/kg/day, Group 1), ethanol and n-acetylcysteine (1 g/kg, Group 2), or isocaloric dextrose(control group, Group 3) for 4 weeks. Then animals weresacrificed under ether anesthesia, intracardiac blood andliver tissues were obtained. Measurements were performedboth in serum and in homogenized liver tissues.Malondialdehyde (MDA) level was measured by TBARSmethod. Glutathione peroxidase (GSH-Px) and superoxidedismutase (SOD) levels were studied by commercial kits.Kruskal-Wallis test was used for statistical analysis.RESULTS: ALT and AST in Group 1 (154 U/Land 302 U/L,respectively) were higher than those in Group 2 (94 U/L and155 U/L) and Group 3 (99 U/L and 168 U/L) (P=0.001 forboth). Serum and tissue levels of MDA in Group 1 (1.84 nmol/mL and 96 nmol/100 mg-protein) were higher than Group 2(0.91 nmol/mL and 64 nmol/100 mg-protein) and Group 3(0.94 nmol/mL and 49 nmol/100 mg-protein) (P<0.001 forboth). On the other hand, serum GSH-Px level in Group 1(8.21 U/g-Hb) was lower than Group 2 (16 U/g-Hb) andGroup 3 (16 U/g-Hb) (P<0.001). Serum and liver tissue levelsof SOD in Group 1 (11 U/mL and 26 U/100 mg-protein)were lower than Group 2 (18 U/mL and 60 U/100 mg-protein)and Group 3 (20 U/mL and 60 U/100 mg-protein) (P<0.001for both).CONCLUSION: This study demonstrated that ethanol-induced liver damage is associated with oxidative stress,and co-administration of n-acetylcysteine attenuates thisdamage effectively in rat model. 展开更多
关键词 Oxidative Stress ACETYLCYSTEINE Animals Erythrocytes Ethanol dosage free Radical Scavengers GLUTATHIONE Glutathione Peroxidase Lipid Peroxidation Liver Male RATS Rats Wistar Research Support Non-U.S. Gov't Superoxide Dismutase Thiobarbituric Acid Reactive Substances
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Synthesis and Antioxidant Properties of Novel Silybin Analogues 被引量:2
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作者 Jing Xu GONG Lin Jia WENG +9 位作者 Feng WANG Yu Bin FENG Chang Xin ZHOU Hai Bo LI Yi Hang WU Xiao Jiang HAO Xiu Mei WU Hua BAI Joachim STOECKIGT Yu ZHAO 《Chinese Chemical Letters》 SCIE CAS CSCD 2006年第4期465-468,共4页
Eight novel silybin analogues (7a-h) were synthesized and their antioxidant properties including the capability of scavenging superoxide anion free radicals and the inhibitory effect on DPPH free radicals were dete... Eight novel silybin analogues (7a-h) were synthesized and their antioxidant properties including the capability of scavenging superoxide anion free radicals and the inhibitory effect on DPPH free radicals were determined. Several synthetic compounds showed comparable antioxidative effect to that of quercetin. 展开更多
关键词 Silybin analogues SYNTHESIS ANTIOXIDANT DPPH inhibition superoxide anion free radical scavenging.
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Prevention of grafted liver from reperfusive injury 被引量:4
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作者 Kai Ma Yang Yu Xian-Min Bu Yan-Jun Li Xian-Wei Dai Liang Wang Yang Dai Hai-Ying Zhao Xiang-Hong Yang Department of General Surgery,Second Clinical College,China Medical University,Shenyang 110003,Liaoning Province,ChinaDepartrnent of Physiology,Shenyang Physical Education College,Shenyang,Liaoning Province,ChinaDepartment of Pathology,China Medical University,Shenyang,Liaoning Province,China 《World Journal of Gastroenterology》 SCIE CAS CSCD 2001年第4期572-574,共3页
INTRODUCTIONThe incidence of primary non-function(PNF)of grafted liver in the early postoperative stage is 2%-23%[1-4],its main cause is the ischemic-rechemic injure[5,6].In this experiment,anisodamine was added into ... INTRODUCTIONThe incidence of primary non-function(PNF)of grafted liver in the early postoperative stage is 2%-23%[1-4],its main cause is the ischemic-rechemic injure[5,6].In this experiment,anisodamine was added into the preserving fluid and the grafted liver was rewarmed at different temperatures to protect the cell membranc and prevent ischemic-reperfusive injury. 展开更多
关键词 Liver Transplantation Animals Body Temperature Drugs Chinese Herbal free Radical Scavengers HEPATOCYTES Lipid Peroxidation LIVER Male Microscopy Electron RATS Rats Wistar Reactive Oxygen Species Reperfusion Injury control Research Support Non-U.S. Gov't Solanaceous Alkaloids Superoxide Dismutase
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Levels of antioxidant enzymes and alkaline protease from pulp and peel of sunflower 被引量:1
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作者 Wesen Adel Mehdi Faridah Yusof +2 位作者 Layla O.Farhan Atheer Awad Mehde Raha Ahmed Raus 《Asian Pacific Journal of Tropical Biomedicine》 SCIE CAS 2017年第6期533-537,共5页
Objective:The activity of enzymes participating in the systems of antioxidant protection was assayed in the peel and pulp of sunflower.The essential roles of proteases in food stimulate research to find other sources ... Objective:The activity of enzymes participating in the systems of antioxidant protection was assayed in the peel and pulp of sunflower.The essential roles of proteases in food stimulate research to find other sources of the enzyme especially from non-conventional sources.In the present work,we study several biochemical parameters in the pulp and peel of sunflower.Methods:Pulp and peel of sunflower was extracted,antioxidant enzymes and nonenzymatic antioxidant were measured.Alkaline protease was measured and purified from pulp in sunflower.Results:High carbohydrate concentration,beta-carotene,catalase and ascorbate peroxidase activities,free radical scavenging capacity and free flavonoid content were observed in the peel of sunflower.Whereas,MDA and ceruloplasmin activities were high in the pulp of sunflower.Conclusions:The present study concluded that peel in sunflower are strong radical scavengers and can be considered as good sources of natural antioxidants for medicinal and commercial uses.Further analysis showed that protease activity was a significantly high in the pulp compared to the peel. 展开更多
关键词 Pulp sunflower Antioxidant enzymes free radical scavenging capacity free flavonoid content Alkaline protease
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Syntheses and Crystal Structures of [Na(H_2O)_(1/2)]X and NH_2(CH_2CH_3)_2X and Antioxidant Activity of the Former 被引量:1
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作者 刘彬 杨伯伦 《Chinese Journal of Structural Chemistry》 SCIE CAS CSCD 2009年第9期1112-1120,共9页
In order to enhance the water-solubility and biological utilization rate of chrysin, sodium 5,7-dihydroxylflavone-8-sulfonate (1, [Na(H2O)1/2]X, X = C15H9OSO3, 5,7-dihydroxylfla- vone-8-sulfonate) was synthesized ... In order to enhance the water-solubility and biological utilization rate of chrysin, sodium 5,7-dihydroxylflavone-8-sulfonate (1, [Na(H2O)1/2]X, X = C15H9OSO3, 5,7-dihydroxylfla- vone-8-sulfonate) was synthesized and its structure was identified on the basis of NMR, FT-IR and elemental analysis. The assembly of 5,7-dihydroxylflavone-8-sulfonate with diethylamide cation afforded diethylamide 5,7-dihydroxylflavone-8-sulfonate (2, NH2(CH2CH3)2X) which was characterized by FT-IR and elemental analysis. The crystal structures of 1 and 2 were determined by X-ray single-crystal diffraction analysis. The crystal of 1 is of triclinic system, space group P1, with a = 8.5628(13), b = 12.8916(19), c = 13.562(2) A, α = 82.494(1), β = 78.601(2), γ = 84.033(2)°, C30H20Na2O15S2, Z = 2, Mr = 730.59, V = 1450.3(4) A3, Dc = 1.673 g/cm3, F(000) = 748, p = 0.295 mm^-1, the final R = 0.0641 and wR = 0.1458. The crystal of 2 crystallizes in the triclinic system, space group Pi, with a = 7.689(2), b = 11.184(3), c = 11.734(3) A, α = 74.268(3), βl = 81.751(4), γ= 87.991(3)°, C19H21NO7S, Z = 2, Mr= 407.43, V= 961.2(4) A3, Dc = 1.408 g/cm3, F(000) = 428, p = 0.210 mm^-1, the final R = 0.0484 and wR = 0.1195. In 1, the three-dimensional structure is organized into organic and inorganic regions; the flavone skeletons are stacked into organic regions by π...π staeking interactions; inorganic regions are generated by Na-O coordination bonds among sulfonate groups, coordinated water molecules and NaI. The sulfonate groups play an important role as a bridge of inorganic and organic regions. One-dimensional chain structure of 2 is extended by N-H…O hydrogen bonds and π...π stacking interactions. Furthermore, the antioxidant activity of 1 was evaluated. The scavenging activity of 1 to DPPH free radical is better than that of the parent compound chrysin. 展开更多
关键词 5 7-dihydroxylflavone-8-sulfonate spectroscopic property crystal structure DPPH free radical scavenging activity
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Edaravone Enhances the Viability of Ischemia/reperfusion Flaps 被引量:1
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作者 张栋益 康深松 +1 位作者 张正文 吴蕊 《Journal of Huazhong University of Science and Technology(Medical Sciences)》 SCIE CAS 2017年第1期51-56,共6页
The purpose of the experiment was to study the efficacy of edaravone in enhancing flap viability after ischemia/reperfusion(IR) and its mechanism. Forty-eight adult male SD rats were randomly divided into 3 groups:... The purpose of the experiment was to study the efficacy of edaravone in enhancing flap viability after ischemia/reperfusion(IR) and its mechanism. Forty-eight adult male SD rats were randomly divided into 3 groups: control group(n=16), IR group(n=16), and edaravone-treated IR group(n=16). An island flap at left lower abdomen(6.0 cm×3.0 cm in size), fed by the superficial epigastric artery and vein, was created in each rat of all the three groups. The arterial blood flow of flaps in IR group and edaravone-treated IR group was blocked for 10 h, and then the blood perfusion was restored. From 15 min before reperfusion, rats in the edaravone-treated IR group were intraperitoneally injected with edaravone(10 mg/kg), once every 12 h, for 3 days. Rats in the IR group and control group were intraperitoneally injected with saline, with the same method and frequency as the rats in the edaravone-treated IR group. In IR group and edaravone-treated IR group, samples of flaps were harvested after reperfusion of the flaps for 24 h. In the control group, samples of flaps were harvested 34 h after creation of the flaps. The content of malondialdehyde(MDA) and activity of superoxide dismutase(SOD) were determined, and changes in organizational structure and infiltration of inflammatory cells were observed by hematoxylin-eosin(HE) staining, apoptotic cells of vascular wall were marked by terminal deoxynucleotidyl transferase-mediated d UTP nick-end labeling(TUNEL) assay, and the apoptotic rate of cells in vascular wall was calculated. The ultrastructural changes of vascular endothelial cells were observed by transmission electron microscopy(TEM). Seven days after the operation, we calculated the flap viability of each group, and marked vessels of flaps by immunohistochemical staining for calculating the average number of subcutaneous vessels. The results showed that the content of MDA, the number of multicore inflammatory cells and apoptotic rate of cells in vascular wall in the edaravone-treated IR group were significantly lower than those in the IR group. The activity of SOD, flap viability and average number of subcutaneous vessels in the edaravone-treated IR group were significantly higher than those in the IR group. All the differences were statistically significant. The ultrastructure injury of vascular endothelial cells in the edaravone-treated IR group was slighter than that in IR group. It was concluded that edaravone can significantly enhance IR flap viability and protect flap vessels, which is related to scavenging oxygen free radicals, reducing the consumption of SOD, reducing the extent of lipid peroxidation and inflammation, and protecting functional structure of vessels in the early stages of reperfusion. 展开更多
关键词 free radical scavengers edaravone ischemia/reperfusion injury skin flap
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Tentative Identification of Phytochemicals and Antioxidant Activities during Fruit-Ripening on Chamaedorea radicalis Mart.
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作者 Gerardo Montelongo-Ruiz Yolanda del Rocio Moreno-Ramirez +3 位作者 Maria Cruz Juarez-Aragon NohemiNino-Garcia Reyna Ivonne Torres-Acosta Torres-Castillo Jorge Ariel 《Phyton-International Journal of Experimental Botany》 SCIE 2020年第2期361-373,共13页
This work aims to determine the phytochemical characterization of the pericarp of Chamaedorea radicalis Mart.fruit as a non-timber product with potential to obtain phytochemicals with potential applications in the ind... This work aims to determine the phytochemical characterization of the pericarp of Chamaedorea radicalis Mart.fruit as a non-timber product with potential to obtain phytochemicals with potential applications in the industry.Fruit from C.radicalis were grouped in four ripening stages named as S1,S2,S3 and S4,according to maturity;S1 the most unripe stage and S4 the completely ripe stage.Determinations of total phenolic compounds,free radical scavenging activities and total flavonoid contents using spectrophotometric methods were done.Also,the tentative identification of phytochemicals during fruit ripening was done using UPLC-MS-MS.Total phenolic compound(TPC)content ranged from 7.24 to 12.53 mg gallic acid equivalents per gram of fresh weight(mg GAE/g FW).Total flavonoids(TF)contents ranged from 0.163 to 0.23 mg of quercetin equivalents per g FW(mg QE/g FW).Free radical scavenging activity against DPPH and ABTS radicals varied from 40.80 to 53.68 and from 22.29 to 37.76 mmol Trolox equivalents g FW(mmol TE/g FW),respectively.Antioxidant assay in vitro by FRAP(ferric reducing antioxidant power)method showed that S3 was the highest level with antioxidant power while S4 was the lowest with Red ripeness stage showed the lowest contents for all determinations.Mass spectrometry allowed detection of 26 compounds,including phenolics,alkaloids and saponins.Afzelin,Kaempferol 3-neohesperidoside and the four saponins identified were present in all ripeness stages.Preliminary phytochemical identification and the spectrophotometric determinations showed that the pericarp of C.radicalis presented antioxidants and compounds related to alkaloids,phenolics and saponins.The presence and abundance of each phytochemical regarding each ripeness stage should be considered. 展开更多
关键词 Metabolite identification PALM free radical scavenging phenolic compounds SAPONINS
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