THE TOXICITY OF TWO NEWLY-SYNTHESIZED COMPOUNDS ON SCENEDESMUS OBLIQUUS KǖTZ

LY-04 and WSQIF are newly-synthesized compounds which were presented by the Institute of Pesticides Chemistry, Central China Normal University. LY-04 belongs to α-oxophosphonic acid derivatives, while WSQIF is an aromatic acetyl oxophosphonic acid derivative. In this study, the toxicity of LY-04 and WSQIF on Scenedesmus obliquus Kütz was examined. The EC 50 of LY-04 and WSQIF were determined in S. obliquus cells at 24h, 48h, 72h, 96h represented by cell density. Our data showed that the 24h, 48h, 72h, 96h EC 50 of LY-04 were 1983mg/L, 2943mg/L, 240mg/L and 65.0mg/L acetone, respectively, and the 24h, 48h, 72h, 96h EC 50 of WSQIF were 15.9mg/L, 53.0mg/L, 98.0mg/L and 28.8mg/L respectively. LY-04 displayed a higher insecticidal activity, while WSQIF showed a higher phytocidal activity. As LY-04 and WSQIF were designed to act as insecticide and herbicide, respectively, the results were in contrast to the anticipated ones. According to the toxicant classification standard, EC 50 of both substances was far higher than 3mg/L, while the toxicity of LY-04 and WSQIF to aquatic algae were fairly low, indicating that these two pesticides are rather safe to the aquatic ecosystem.

亚硝态氮对铜绿微囊藻和四尾栅藻生长的影响

采用室内培养法,将铜绿微囊藻和四尾栅藻分别置于5种含不同质量浓度亚硝态氮（NO2^--N）（0.5 mg/L、1.0 mg/L、2.0 mg/L、4.0mg/L和8.0 mg/L）的培养液中,培养10 d,测定不同质量浓度NO2^--N对铜绿微囊藻和四尾栅藻生长的影响。结果表明,0.5-8.0 mg/L的NO2^--N可激活铜绿微囊藻的亚硝酸氧化酶和亚硝酸还原酶,进而促进铜绿微囊藻的生长。四尾栅藻因仅具有亚硝酸还原酶,因此只能利用0.5-2.0 mg/L的NO2^--N,其利用能力远没有铜绿微囊藻强。培养8 d后,铜绿微囊藻培养液中NH4^＋-N和NO2^--N的质量浓度明显升高,而NO3^--N质量浓度的变化不大;四尾栅藻培养液中NH4^＋-N、NO2^--N和NO3^--N质量浓度的变化不大。研究表明,低于8.0 mg/L的NO2^--N可促进铜绿微囊藻的生长,高于2.0 mg/L的NO2^--N抑制四尾栅藻的生长。