Background : The objective of this study was to validate an animal model for dry eye during and after the administration of 1% ophthalmic atropine sulfate(OAS) in New Zealand white(NZW) rabbits.Methods : OAS(1%) was a...Background : The objective of this study was to validate an animal model for dry eye during and after the administration of 1% ophthalmic atropine sulfate(OAS) in New Zealand white(NZW) rabbits.Methods : OAS(1%) was applied three times per day to 30 eyes of 15 healthy NZW rabbits. Sacrifice, enucleation, and lacrimal gland removal took place on days 15, 21,and 30(OAS group). A second group(n = 5) was used as control. Clinical evaluations took place on days 3, 10, 15, 18, 21, 24 and 30. The primary endpoints were:Schirmer I test, tear break-up time(TBUT), and corneal fluorescein staining. As secondary endpoints, clinical changes including intraocular pressure, and histopathology were evaluated.Results : While OAS was administered, the Schirmer I test showed a statistically significant reduction for OAS group versus control( p < 0.001), and versus basal production( p < 0.001). TBUT showed statistically significant differences between groups(days 3 and 10;p = 0.001) and versus basal values(day 3;p < 0.001). Fluorescein staining showed a statistically significant difference(day 3;p = 0.001). The most frequent clinical finding was conjunctival hyperemia(76.9% OAS vs. 20% control). For histopathology, all OAS subjects presented some degree of inflammation(86.7% minimal;13.3% mild) whereas the control presented only 30% minimal inflammation. Goblet cell density showed no difference.Conclusions : The effectiveness of the OAS dry eye model in NZW rabbits as reported in previous studies was confirmed, provided that the application of the drug is maintained throughout the intervention;it is not a viable model after OAS administration is suspended.展开更多
目的观察针刺配合中药及人工泪液治疗肝肾阴亏型泪液缺乏性干眼症的临床疗效。方法将80例肝肾阴亏型泪液缺乏性干眼症患者随机分为A组30例、B组25例和C组25例。A组采用针刺配合中药及人工泪液治疗,B组采用中药配合人工泪液治疗,C组采用...目的观察针刺配合中药及人工泪液治疗肝肾阴亏型泪液缺乏性干眼症的临床疗效。方法将80例肝肾阴亏型泪液缺乏性干眼症患者随机分为A组30例、B组25例和C组25例。A组采用针刺配合中药及人工泪液治疗,B组采用中药配合人工泪液治疗,C组采用单纯人工泪液治疗。观察各组治疗前后视力、泪液分泌试验(Schirmer I test, SIT)、泪膜破裂时间(break-up time, BUT)、角膜荧光染色(fluorescein staining, FL)、眼部症状评分及中医证候评分的变化情况,并比较各组临床疗效。结果 A组治疗后双眼视力与同组治疗前比较,差异均具有统计学意义(P<0.01);B组治疗后左眼视力与同组治疗前比较,差异具有统计学意义(P<0.05)。各组治疗后双眼SIT、BUT、FL评分与同组治疗前比较,差异均具有统计学意义(P<0.01)。A组治疗后双眼视力评分、SIT与B组和C组比较,差异均具有统计学意义(P<0.01,P<0.05)。A组和B组治疗后双眼BUT、FL评分与C组比较,差异均具有统计学意义(P<0.05,P<0.01)。B组治疗后右眼FL评分与C组比较,差异具有统计学意义(P<0.05)。A组和B组治疗后各项眼部症状(干涩感、异物感、疲劳感)评分与同组治疗前比较,差异均有统计学意义(P<0.01);C组治疗后干涩感、疲劳感评分与同组治疗前比较,差异均有统计学意义(P<0.01,P<0.05)。A组治疗后各项眼部症状评分与C组比较,差异均具有统计学意义(P<0.01);B组治疗后干涩感评分与C组比较,差异具有统计学意义(P<0.05)。A组总有效率为93.3%,B组为80.0%,C组为40.0%;A组中医证候总有效率为90.0%,B组为80.0%,C组为8.0%。A组和B组总有效率及中医证候总有效率与C组比较,差异均具有统计学意义(P<0.01)。结论针刺配合中药及人工泪液是一种治疗肝肾阴亏型泪液缺乏性干眼症的有效方法,可缓解患者症状,提高视力。展开更多
目的观察两种不同的表面麻醉剂对基础泪液分泌试验(Schirmer I test,SIT)的影响。方法选取我院准分子门诊就诊患者84例(168眼),其中男51例(102眼),女33例(66眼),同一患者左右眼随机分别以5 g·L-1盐酸丙美卡因滴眼液和4g·L-1...目的观察两种不同的表面麻醉剂对基础泪液分泌试验(Schirmer I test,SIT)的影响。方法选取我院准分子门诊就诊患者84例(168眼),其中男51例(102眼),女33例(66眼),同一患者左右眼随机分别以5 g·L-1盐酸丙美卡因滴眼液和4g·L-1盐酸奥布卡因滴眼液1滴单次滴眼,观察滴眼后的眼部刺激症状及结膜充血情况,测量其SIT值,并对结果进行比较分析。结果盐酸丙美卡因组有15眼出现一过性眼部刺激症状,盐酸奥布卡因组有33眼,两组比较差异有统计学意义(P<0.05);盐酸丙美卡因组轻度结膜充血的眼数为54眼,明显多于盐酸奥布卡因组的39眼(P<0.05);盐酸丙美卡因组SIT值为(7.50±5.15)mm,盐酸奥布卡因组为(9.71±5.46)mm,两组间比较差异有统计学意义(P<0.05);盐酸丙美卡因组48眼SIT值小于10 mm,盐酸奥布卡因组为33眼,两者间差异有统计学意义(P<0.05);盐酸丙美卡因组30眼SIT值小于5 mm,盐酸奥布卡因组为21眼,两者间差异无统计学意义(P>0.05)。结论盐酸丙美卡因较盐酸奥布卡因有更好的眼部耐受性,且测量的SIT值短于盐酸奥布卡因组,说明盐酸丙美卡因眼表麻醉效果可能要强于盐酸奥布卡因。展开更多
基金sponsored by Laboratorios Sophia,SA de CV(Zapopan,Jalisco,Mexico)。
文摘Background : The objective of this study was to validate an animal model for dry eye during and after the administration of 1% ophthalmic atropine sulfate(OAS) in New Zealand white(NZW) rabbits.Methods : OAS(1%) was applied three times per day to 30 eyes of 15 healthy NZW rabbits. Sacrifice, enucleation, and lacrimal gland removal took place on days 15, 21,and 30(OAS group). A second group(n = 5) was used as control. Clinical evaluations took place on days 3, 10, 15, 18, 21, 24 and 30. The primary endpoints were:Schirmer I test, tear break-up time(TBUT), and corneal fluorescein staining. As secondary endpoints, clinical changes including intraocular pressure, and histopathology were evaluated.Results : While OAS was administered, the Schirmer I test showed a statistically significant reduction for OAS group versus control( p < 0.001), and versus basal production( p < 0.001). TBUT showed statistically significant differences between groups(days 3 and 10;p = 0.001) and versus basal values(day 3;p < 0.001). Fluorescein staining showed a statistically significant difference(day 3;p = 0.001). The most frequent clinical finding was conjunctival hyperemia(76.9% OAS vs. 20% control). For histopathology, all OAS subjects presented some degree of inflammation(86.7% minimal;13.3% mild) whereas the control presented only 30% minimal inflammation. Goblet cell density showed no difference.Conclusions : The effectiveness of the OAS dry eye model in NZW rabbits as reported in previous studies was confirmed, provided that the application of the drug is maintained throughout the intervention;it is not a viable model after OAS administration is suspended.
文摘目的观察针刺配合中药及人工泪液治疗肝肾阴亏型泪液缺乏性干眼症的临床疗效。方法将80例肝肾阴亏型泪液缺乏性干眼症患者随机分为A组30例、B组25例和C组25例。A组采用针刺配合中药及人工泪液治疗,B组采用中药配合人工泪液治疗,C组采用单纯人工泪液治疗。观察各组治疗前后视力、泪液分泌试验(Schirmer I test, SIT)、泪膜破裂时间(break-up time, BUT)、角膜荧光染色(fluorescein staining, FL)、眼部症状评分及中医证候评分的变化情况,并比较各组临床疗效。结果 A组治疗后双眼视力与同组治疗前比较,差异均具有统计学意义(P<0.01);B组治疗后左眼视力与同组治疗前比较,差异具有统计学意义(P<0.05)。各组治疗后双眼SIT、BUT、FL评分与同组治疗前比较,差异均具有统计学意义(P<0.01)。A组治疗后双眼视力评分、SIT与B组和C组比较,差异均具有统计学意义(P<0.01,P<0.05)。A组和B组治疗后双眼BUT、FL评分与C组比较,差异均具有统计学意义(P<0.05,P<0.01)。B组治疗后右眼FL评分与C组比较,差异具有统计学意义(P<0.05)。A组和B组治疗后各项眼部症状(干涩感、异物感、疲劳感)评分与同组治疗前比较,差异均有统计学意义(P<0.01);C组治疗后干涩感、疲劳感评分与同组治疗前比较,差异均有统计学意义(P<0.01,P<0.05)。A组治疗后各项眼部症状评分与C组比较,差异均具有统计学意义(P<0.01);B组治疗后干涩感评分与C组比较,差异具有统计学意义(P<0.05)。A组总有效率为93.3%,B组为80.0%,C组为40.0%;A组中医证候总有效率为90.0%,B组为80.0%,C组为8.0%。A组和B组总有效率及中医证候总有效率与C组比较,差异均具有统计学意义(P<0.01)。结论针刺配合中药及人工泪液是一种治疗肝肾阴亏型泪液缺乏性干眼症的有效方法,可缓解患者症状,提高视力。
