Chemical Constituents of Schisandra rubriflora Rehd. et Wils.

Abstract: Schisandra rubriflora Rehd. et Wils. is a traditional Chinese medicine. To search for new and bioactive components from traditional Chinese medicines and provide scientific evidence for taxonomy, the chemical constituents of the plant were investigated by various column chromatography methods (silica gel, Sephadex LH-20, and RP-18). From the aerial parts of S. rubriflora, three new megastigmane glycosides, namely (3S, 5R, 6S, 9R)-megastigmane-3, 9-diol 3-O-[α-L-arabionfuranosyl-(1→6)-β-D-glucopyranoside] (1), 7-megastigmene-3-ol-9-one 3-O-[α-L-arabionfuranosyl-(1→6)-β-D-glucopyranoside] (2), and megastigmane-3α, 4β, 9σ-triol 3-O-β-D-glucopyranoside (3), along with 14 known compounds, were isolated. The structures of the new compounds were elucidated by a combination of spectroscopic and chemical methods.

HPLC法测定红花五味子木脂素的含量

目的 测定不同产地、不同部位红花五味子的五味子酯甲 ( schisantherin A)、去氧五味子素 (五味子甲素 ,deoxyschizandrin)、五味子乙素 ( schisandrin B)和五味子丙素 ( schisandrin C)含量。方法 HPL C法 ,使用 Sphere-clone ODS柱 ;流动相 :水 ( A) ,甲醇 ( B) ,梯度洗脱 :0～ 4 m in,70 % B;4～ 5 4 m in,70 %～ 10 0 % B;流速为 0 .4 m L/min;柱温 :2 5℃ ;检测波长 :2 5 4 nm。结果 五味子酯甲、甲素、乙素、丙素在红花五味子中含量分别为 0 .0 2 6 %～0.0 8 3% ,0 .0 0 7%～ 0 .94 5 % ,0 .0 0 2 %～ 0 .12 1% ,0 .0 10 %～ 0 .0 38% ,其中五味子甲素普遍存在 ,且果实中含量高于茎藤。结论 去氧五味子素是红花五味子果实的主要活性成分。

秦岭地区四种五味子果实木脂素检测

目的:测定秦岭地区华中五味子、大花五味子、红花五味子、铁箍散果实4种木脂素含量。方法:采用超声提取木脂素,HPLC检测,Diamonsil C18色谱柱(150 mm×4.6 mm,5μm),流动相为甲醇-水(70∶30),流速为0.8mL/min,检测波长为280 nm。结果:五味子醇甲、五味子甲素和五味子乙素在4种五味子果实中均存在,华中五味子中五味子酯甲含量达到2010年版中国药典规定,大花五味子、红花五味子中五味子甲素含量较高,而铁箍散中五味子乙素含量较高。结论:秦岭五味子属4种五味子果实中木脂素含量差异较大,可为进一步研究提供参考。

南五味子类药材的鉴别研究

目的 为南五味子药材的鉴定提供依据。方法 用性状鉴别、扫描电镜和薄层色谱方法对不同产地华中五味子及其近缘种绿叶五味子、翼梗五味子和红花五味子果实进行生药性状、种子表面微形态和薄层鉴别。结果 依据种皮表面的微形态特征可以将华中五味子、红花五味子、绿叶五味子及翼梗五味子分为 3个类型。去氧五味子素(五味子甲素 ) (deoxyschisandrin)、五味子酯甲 (schisantherin A)的薄层定性表明产于陕西平利、河南栾川、山西阳城、湖南衡山的华中五味子、绿叶五味子和红花五味子果实中都含有五味子酯甲和五味子甲素 ;翼梗五味子果实中仅含有五味子酯甲 ,而甘肃小陇山、陕西留坝产华中五味子果实中检测不到这两种成分。结论 不同产地华中五味子、红花五味子、绿叶五味子及翼梗五味子的干燥果实可以依据种皮表面的微形态特征和薄层色谱结果加以鉴别。

红花五味子茎低极性化学成分的GC-MS分析

目的研究红花五味子茎低极性部位的主要化学成分。方法红花五味子茎用90%乙醇热提取,提取液用石油醚萃取得到石油醚部位,运用GC-MS法对其主要成分进行检测,所测结果与标准谱库进行分析,用峰面积归一法计算各个成分的相对含量。结果鉴定了49种化学成分,相对含量较高的有β-芹子烯,约占12.08%、顺,顺-2-甲基-3,13-十八烯醇,约占10.3%。结论红花五味子茎低极性部位化学成分主要为酯类化合物(45.94%),其次为脂肪酸(19.35%)和倍半萜(13.94%)。

苗药五香血藤的质量测定

目的:建立五香血藤药材的质量测定,为五香血藤的质量标准研究和修订提供借鉴基础。方法:测定药材水分、总灰分和酸不溶性灰分;采用高效液相色谱法测定药材中五味子乙素的含量:色谱柱为Eclipse XDB-C18,流动相为乙腈-水(80∶20,V/V),流速为1.0 m L/min,检测波长为235 nm,柱温为30℃,进样量为20μL。结果:药材水分为10.74~14.49%,总灰分为2.12~4.30%,酸不溶性灰分为0.18~3.16%。五味子乙素检测进样量线性范围为2.10~12.62μg(r=0.9999);精密度、稳定性、重复性试验的RSD≤3%;五香血藤素加样回收率为102.27%(RSD=2.6%)。结论:该研究所建标准可用于五香血藤药材的质量控制。

Structural determination of eleven new preschisanartane-type schinortriterpenoids from two Schisandra species and structural revision of preschisanartanin J using NMR computation method

Nineteen preschisanartane-type schinortriterpenoids(SNTs),among which eleven ones were previously undescribed,were isolated from two Schisandra species,S.sphaerandra and S.rubriflora.Their structures were determined using 1 D and 2 D NMR spectroscopic analyses,NMR data comparison,quantum chemical calculation of NMR parameters,electronic circular dichroism(ECD),X-ray single crystal diffraction,and chemical derivation.Furthermore,structural re-examination of a few previously reported preschisanartane-type SNTs led to the structural revision of preschisanartanin J.Besides,it is suggested that the reported structures of arisanlactone D and schilancidilactone W should be re-checked.Finally,a few isolated SNTs were found to possess neurite outgrowthpromoting activities,and protective activities against neural injuries.

红花五味子中四个新的联苯环辛二烯类木脂素(英文)

目的:研究湖北恩施地区产红花五味子茎干的化学成分。方法:采用硅胶和RP-18等层析柱分离手段以及半制备和制备高效液相,运用2D-NMR,HR-ESI-MS以及圆二色谱等波谱技术鉴定化合物的结构。结果:从红花五味子中分离鉴定出4个新的联苯环辛二烯类木脂素并命名为schirubrisinsA-D(1-4),另外还有14个已知化合物。结论:化合物1-4是新的联苯环辛二烯类木脂素。