SDS-PAGE PROTEIN PATTERN AND ITS ANTIGENICITY ANALYSIS OF DIFFERENT ISOLATES OF SCHISTOSOMA JAPONICUM IN CHINA

Homogenates prepared from S. japonicum adult worms ofdifferent isolates from Anhui, Hubei, Guangxi, Yunnan andSichuan Provinces were analyzed by SDS-PAGE and enzymelinked immunoelectrotransfer blot (EITB) tested with rabbitanti-snails antibody. The results of SDS-PAGE indicated thatwith silver staining both male and female worms of Guangxiisolate showed some definite differences in their protein profile,namely, absence of one band between 50-75 kDa in maleworms and marked reduction in quantity of > 110 and 30 kDabands in female worms. There was no obvious differenceamong other isolates both in male and female worms. TheEITB patterns were similar in S. japonicum of Anhui andHubei, and it was also the case with isolates from Yunnan andSichuau, except that Yuunan female worms had a distinct band

日本血吸虫未成熟卵可溶性抗原的初步分析

采用ＳＤＳ－ＰＡＧＥ和ＥＬＩＢ技术分析了日本血吸虫未成熟卵可溶性抗原（ＳＩＥＡ），并与可溶性成熟卵抗原（ＳＥＡ）进行比较，二者主要蛋白区带存在明显差异。同时，ＥＬＩＢ结果表明，ＳＩＥＡ中感染血清和免疫血清所识别的６７ｋＤａ、６２ｋＤａ、５４ｋＤａ、５０ｋＤａ、２８ｋＤａ和２６ｋＤａ等抗原组分不出现于ＳＥＡ，推测其在ＳＩＥＡ诱导宿主产生抗雌虫生殖及抗卵胚发育免疫应答方面起作用。

不同地区日本血吸虫成虫可溶性抗原免疫反应性的比较

应用酶联免疫印迹技术检测我国不同地区日本血吸虫雌、雄及雌雄合抱虫体可溶性抗原(AWA),结果上述三者与相应感染动物血清及慢性日本血吸虫病人血清反应,分别显示出4～20、6～34和3～29条反应带;一地日本血吸虫AWA与各地感染鼠血清反应呈明显相似,而不同地区虫体AWA与各感染鼠血清反应性之间则显示出大的差异性;与不同感染宿主血清(感染鼠、兔和病人血清)的反应带及其分子量互不相同;各地雌、雄虫之间也不同,一般雄虫较雌虫反应带多且强;不同宿主(兔和鼠)体内获取的江苏虫体AWA的反应性也存有差异。提示不同地区日本血吸虫成虫可溶性抗原的血清反应性存在着地区及性别间差异。

日本血吸虫未成熟卵可溶性抗原的分析

采用免疫沉淀技术和ＥＩＴＢ技术分析了日本血吸虫未成熟卵可溶性抗原（ＳＩＥＡ）。结果：能被ＳＩＥＡ免疫血清识别的ＳＩＥＡ带主要集中于３０８ＫＵ到４９６ＫＵ之间，其主要反应带为４９６ＫＵ、３９７ＫＵ和３３７ＫＵ。推测４９６ＫＵ和３３７ＫＵ这两抗原分子在诱导宿主产生抗卵成熟过程中可能起着重要作用。