Synthesis of New Schizandrin Analogues

Two new lignans with dibenzocycloheptadiene skelectons have been synthesized by intramolecular nonphenolic oxidative coupling and rearrangement. The structures of these products have been identified by MS, UV, IR and NMR spectra.

Synthesis of three new Schizandrin Analogues

Three new Schizandrin analogues with dibenzocycloheptatriene skeletons have been synthesized. Their structures were identified by MS, UV, IR and NMR spectral data.

Synergistic effect of schizandrin A and DNaseⅠknockdown on high glucose induced beta cell apoptosis by decreasing intracellular calcium concentration

OBJECTIVE:To explore the synergistic effect of deoxyribonucleaseⅠ(DNaseⅠ)knockdown combined with Schizandrin A(Sch A)in protecting islet beta-cells(β-cells)from apoptosis under high-glucose(HG)conditions.METHODS:The concentration of Sch A was detected by Cell Counting Kit-8(CCK-8).High glucose-cultured rat insulinoma beta cell line(RIN-M5F)cells were treated with Sch A and transfected with DNaseⅠsmall interfering RNA(siRNA).Cell apoptosis rate and apoptosis-related protein level were examined by flow cytometry and Western blot method respectively.In addition,Na^(+)-K^(+)-adenosine triphosphatease(Na^(+)-K^(+)-ATPase)and Ca^(2+)-Mg^(2+)-ATPase activity,cell membrane potential,and intracellular Ca^(2+)concentration was also examined respectively.RESULTS:Our study revealed that HG stimulation can cause a significant increase in DNaseⅠlevel and cell apoptosis rate.However,Sch A combined with DNaseⅠknockdown can significantly decrease the cell apoptosis rate and apoptosis-related protein levels such as BAX(P<0.05)and Caspase-3(P<0.01).In addition,we also found that the combination of Sch A and DNaseⅠknockdown can dramatically increase cell membrane potential level,Na+-K+-ATPase,and Ca^(2+)-Mg^(2+)-ATPase activity.Meanwhile,intracellular Ca^(2+)concentration was also found to be significantly decreased by the synergistic effect of Sch A and DNaseⅠknockdown.CONCLUSION:Overall,our study reveals a synergistic effect of Sch A and DNaseⅠknockdown in protectingβ-cells from HG-induced apoptosis.

Second-order calibration applied to quantification of two active components of Schisandra chinensis in complex matrix

The effectiveness of traditional Chinese medicine (TCM) against various diseases urges more low cost, speed and sensitive analytical methods for investigating the phamacology of TCM and providing a theoretical basis for clinical use. The potential of second-order calibration method was validated for the quantification of two effective ingredients of Schisandra chinensis in human plasma using spectrofluorimetry. The results obtained in the present study demonstrate the advantages of this strategy for multi-target determination in complex matrices. Although the spectra of the analytes are similar and a large number of interferences also exist, second-order calibration method could predict the accurate concentrations together with reasonable resolution of spectral profiles for analytes of interest owing to its ‘second-order advantage’. Moreover, the method presented in this work allows one to simply experimental procedure as well as reduces the use of harmful chemical solvents.

Optimization of Smashing Tissue Extraction Technology of Schisandra chinensis Fruits by Orthogonal Test

Objective To optimize the extract technology of active lignins from the fruits of Schisandra chinensis. Methods The content of schizandrin, gomisin A, and deoxyschizandrin were selected as standards to evaluate the efficiency of smashing tissue extraction (STE). Solid-liquid ratio, extracting times, ethanol concentration, and extracting time were investigated through orthogonal test. Results The optimized conditions for STE were ten times amount of 80% EtOH, extracting for three times, and 2 min for each time. Conclusion STE could obtain relatively higher yield, simplicity of operation, and benefit for environment protection. It could be better choice for the extraction of S. chinensis.