From the methanol extract of the whole plant of Schnabelia oligophylla Hand.-Mazz. (Lamiaceae), a new cyclic octopeptide, named schnabepeptide (1), was isolated by silica gel chromatography. Its structure was elucidat...From the methanol extract of the whole plant of Schnabelia oligophylla Hand.-Mazz. (Lamiaceae), a new cyclic octopeptide, named schnabepeptide (1), was isolated by silica gel chromatography. Its structure was elucidated by spectroscopic analyses and the absolute configuration of the amino acid units, except for proline and glycine, were assigned by chiral HPLC analysis. Bio-assay showed that schnabepeptide (1) exhibited an immunosuppression activity on T/B lymphocytes. Along with the new compound, seven known compounds, octadeca-9, 16-dioxo-10(E),12(Z),14(E)-trienoic acid (2), 12, 16-epoxy-11, 14-dihydroxy-17( 15-->16), 18 (4-->3)-diabeo-abieta-3,5,8,11,13,15-hexene-2, 7-dione (teuvincenone F, 3), abscisic acid (4), beta -sitosterol (5), daucosterol (6), stigmasteryl 3-O-beta -D-glucopyranoside (7) and palmitic acid (8), were also isolated from this plant.展开更多
A new cyclic octapeptide (schnabepeptide B) was isolated from the aerial part of Schnabelia tetradonta (Sun) C. Y. Wu et C. Chen (Lamiaceae). Its structure was elucidated as cyclo-(NH-Trp-Gly1-Leu1-Gly2-Pro1-Pro2-Leu2...A new cyclic octapeptide (schnabepeptide B) was isolated from the aerial part of Schnabelia tetradonta (Sun) C. Y. Wu et C. Chen (Lamiaceae). Its structure was elucidated as cyclo-(NH-Trp-Gly1-Leu1-Gly2-Pro1-Pro2-Leu2-Pro3-CO) on the basis of extensive 2D NMR and MS spectra.展开更多
The chloroplast gene matK and the internal transcribed spacers (ITS) of the nuclear ribosomal DNA from Schnabelia, a genus endemic to China, and 6 genera of Verbenaceae and 13 genera of Lamiaceae were sequenced. The p...The chloroplast gene matK and the internal transcribed spacers (ITS) of the nuclear ribosomal DNA from Schnabelia, a genus endemic to China, and 6 genera of Verbenaceae and 13 genera of Lamiaceae were sequenced. The phylogenetic signal and validity outgroups were measured and evaluated by means of the relatively apparent synapomorphy analysis (RASA). Independent and combined phylogenetic analyses for the matK and ITS sequences were performed using the maximum parsimony (MP), neighbor- joining (NJ) and maximum likelihood (ML) methods, indicating that Schnabelia oligophylla and Caryopteris terniflora form a sister-group relationship. The Caryopteris complex is not shown to be a monophyly because Trichostema, C. paniculata and C. forrestii are paraphyletic to the clade containing the remaining members of the complex. A monophyly of Ajugoideae proposed by Cantino et al., including 8 genera in this study, is strongly supported and the closest relatives of Schnabelia are in the Ajugoideae (Lamiaceae), especially near Caryopteris terniflora. The polygenetic analyses also showed that the genera of Lamiaceae and Verbenaceae sampled in this tudy are phylogenetically mixed and the genus Avicennia is distant to other genera of Verbenaceae. RASA and combined analysis can be used as effective approaches to determining the relationships among phylogenetically complex groups.展开更多
通过研究料液比、乙醇体积分数、提取温度和超声时间对筋骨草[Schnabelia tetradonta(Sun)C.Y.Wu et C.Chen]中生物碱得率的影响,优选出超声最佳工艺,并以盐酸小檗碱为标准对照测定野生和家种筋骨草生物碱的得率。结果表明,生物碱的最...通过研究料液比、乙醇体积分数、提取温度和超声时间对筋骨草[Schnabelia tetradonta(Sun)C.Y.Wu et C.Chen]中生物碱得率的影响,优选出超声最佳工艺,并以盐酸小檗碱为标准对照测定野生和家种筋骨草生物碱的得率。结果表明,生物碱的最佳提取工艺为药材加25倍的60%乙醇溶液(p H=1),50℃水浴1 h后超声30 min,得到野生筋骨草生物碱得率为0.072%,家种筋骨草生物碱得率为0.099%。该提取工艺合理,生物碱得率较高,为筋骨草生物碱的提取提供参考依据。展开更多
文摘From the methanol extract of the whole plant of Schnabelia oligophylla Hand.-Mazz. (Lamiaceae), a new cyclic octopeptide, named schnabepeptide (1), was isolated by silica gel chromatography. Its structure was elucidated by spectroscopic analyses and the absolute configuration of the amino acid units, except for proline and glycine, were assigned by chiral HPLC analysis. Bio-assay showed that schnabepeptide (1) exhibited an immunosuppression activity on T/B lymphocytes. Along with the new compound, seven known compounds, octadeca-9, 16-dioxo-10(E),12(Z),14(E)-trienoic acid (2), 12, 16-epoxy-11, 14-dihydroxy-17( 15-->16), 18 (4-->3)-diabeo-abieta-3,5,8,11,13,15-hexene-2, 7-dione (teuvincenone F, 3), abscisic acid (4), beta -sitosterol (5), daucosterol (6), stigmasteryl 3-O-beta -D-glucopyranoside (7) and palmitic acid (8), were also isolated from this plant.
基金This research was supported by the grant from the Special Project of Biological Science and Technology of the Chinese Academy of Sciences(STZ 97-3-08).
文摘A new cyclic octapeptide (schnabepeptide B) was isolated from the aerial part of Schnabelia tetradonta (Sun) C. Y. Wu et C. Chen (Lamiaceae). Its structure was elucidated as cyclo-(NH-Trp-Gly1-Leu1-Gly2-Pro1-Pro2-Leu2-Pro3-CO) on the basis of extensive 2D NMR and MS spectra.
基金supported by funds from the Arnold Arboretum of Harvard University for fieldwork in the U.S.A.the National Science Foundation for Distinguished Young Scholars(Grant No.39825104)+2 种基金the National Natural Science Foundation of China(Grant Nos.30070053,30170071 and 30230030)the Natural Science Foundation of Guangdong Province(Grant No.001223)the Ministry of Education Special Foundation(Grant No.20010558013).
文摘The chloroplast gene matK and the internal transcribed spacers (ITS) of the nuclear ribosomal DNA from Schnabelia, a genus endemic to China, and 6 genera of Verbenaceae and 13 genera of Lamiaceae were sequenced. The phylogenetic signal and validity outgroups were measured and evaluated by means of the relatively apparent synapomorphy analysis (RASA). Independent and combined phylogenetic analyses for the matK and ITS sequences were performed using the maximum parsimony (MP), neighbor- joining (NJ) and maximum likelihood (ML) methods, indicating that Schnabelia oligophylla and Caryopteris terniflora form a sister-group relationship. The Caryopteris complex is not shown to be a monophyly because Trichostema, C. paniculata and C. forrestii are paraphyletic to the clade containing the remaining members of the complex. A monophyly of Ajugoideae proposed by Cantino et al., including 8 genera in this study, is strongly supported and the closest relatives of Schnabelia are in the Ajugoideae (Lamiaceae), especially near Caryopteris terniflora. The polygenetic analyses also showed that the genera of Lamiaceae and Verbenaceae sampled in this tudy are phylogenetically mixed and the genus Avicennia is distant to other genera of Verbenaceae. RASA and combined analysis can be used as effective approaches to determining the relationships among phylogenetically complex groups.
