A new sesterterpenoid phellogine was isolated from the fruits of Phellodendron chinense var. glabriusculum Schneid. Its structure was elucidated on the basis of spectral analysis.
[Objective]This study aimed to observe the antibacterial activity of Berberis julianae Schneid and Cinnamomum camphora（ L.） Presl. aqueous extracts and their antibiotical effect when being used with commonly used an...[Objective]This study aimed to observe the antibacterial activity of Berberis julianae Schneid and Cinnamomum camphora（ L.） Presl. aqueous extracts and their antibiotical effect when being used with commonly used antibiotics in- vitro. [Method] Broth microdilution method and checkerboard dilution method were used and the reference strain E. coli ATCC25922 and clinical sample separation strains of E. coli and Salmonella were selected to determine the minimum inhibitory concentration（ MIC） and fractional inhibitory concentration（ FIC）. [Result] The MICs of Berberis julianae Schneid and Cinnamomum camphora（ L.） Presl. for E. coli and Salmonella were both 1 mg /mL,and that of other antibiotics for the two strains were in the quality control range provided by NCCLS. [Conclusion] The synergistic effect or additive effect were observed when they were applied with the commonly used antibiotics.展开更多
[Objectives] To establish the quality standard of barberry branches. [Methods] Microscopic identification and thin layer identification were used to qualitatively identify barberry branches. Berberine content was dete...[Objectives] To establish the quality standard of barberry branches. [Methods] Microscopic identification and thin layer identification were used to qualitatively identify barberry branches. Berberine content was determined by HPLC method, and the content of water, total ash, acid insoluble ash and extract was detected according to the method of the Chinese Pharmacopoeia (2020 edition). [Results] The microscopic identification showed that the features were obvious, and stone cells, cork cells, epidermal cells, stomata, fibers and catheter with reticulated pores could be found. Berberine was detected in barberry branches by thin layer chromatography, and the characteristic spots were separated clearly. Moisture, total ash, and acid insoluble ash content shall not exceed 13%, 6%, and 3%, respectively, and extract content shall not be less than 10%. Berberine hydrochloride (C_(20)H_(18)ClNO_(4)) should be calculated in the branches of Berberis wilsonae Hemsley and Berberis aggregata C. K. Schneid., and berberine (C_(20)H_(17)NO_(4)) content should not be less than 0.05%. The linear relationship was good in the range of 0.002-0.240 mg/mL ( R^(2)=0.999 5). The average recovery was 89.63%, and RSD was 5.28%. [Conclusions] The method was simple, accurate and reproducible, and can be used for the quality control of barberry branches.展开更多
目的 基于网络药理学和分子对接探讨“寒凉”中药黄连、黄芩、黄柏(三黄)治疗痴呆的作用机制。方法 通过数据挖掘《宣明论方》,筛选出符合“寒凉”属性并且是治疗“痴呆症”的常见中药黄芩、黄连、黄柏。通过中药系统药理学数据库与分...目的 基于网络药理学和分子对接探讨“寒凉”中药黄连、黄芩、黄柏(三黄)治疗痴呆的作用机制。方法 通过数据挖掘《宣明论方》,筛选出符合“寒凉”属性并且是治疗“痴呆症”的常见中药黄芩、黄连、黄柏。通过中药系统药理学数据库与分析平台(Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform, TCMSP)数据库检索“三黄”的有效成分和作用靶点,在GeneCards、DrugBank等数据库检索痴呆症(dementia)靶点。利用Cytoscape3.7.2构建“药物-成分-靶点”网络,用VENNY2.1获得“三黄”和痴呆症的交集靶点后,构建蛋白质互作网络(protein-protein interaction networks, PPI),利用Metascape库对核心靶点进行GO、KEGG富集分析,并构建“三黄”药物-成分-靶点-通路-疾病网络,最后利用分子对接,以反向验证“三黄”活性成分与核心靶点之间的关系。结果 筛选出“三黄”治疗痴呆症的重要活性成分有槲皮素、β-谷甾醇、小檗碱、黄连碱等,关键核心靶点有TP53、AKT1、IL6、JUN、MAPK14、TNF等。KEGG通路分析有129条,主要有AGE-RAGE信号介导的糖尿病并发症通路、PI3K-Akt信号通路、钙信号通路等;GO功能中,分子功能117条,细胞组成61条,生物过程1 366条,主要涉及配体激活转录因子活性、DNA结合转录因子结合等分子功能,膜微区、突触后膜等细胞组成,活性氧代谢过程、对脂多糖的反应、炎症反应等生物过程。分子对接结果可以看出重要活性成分与核心靶点全部对接成功。结论 本研究结果表明,“三黄”特有的药性优势,可以通过多成分、多靶点、多通路的方式治疗痴呆症,为“三黄”治疗痴呆症临床应用和机制研究提供了依据。展开更多
基金Talent Special Fund of Guizhou Province(No.200560 and No.2005247)for financial supportedprofessor Jianxin Zhang of analytical group of The Key Laboratory of Chemistry for Natural Products of Guizhou Province and Chinese Academy of Sciences for the spectral measurements.
文摘A new sesterterpenoid phellogine was isolated from the fruits of Phellodendron chinense var. glabriusculum Schneid. Its structure was elucidated on the basis of spectral analysis.
基金Funded by the Student Innovation Project of Southwest University for Nationalities in 2012(201210656017)
文摘[Objective]This study aimed to observe the antibacterial activity of Berberis julianae Schneid and Cinnamomum camphora（ L.） Presl. aqueous extracts and their antibiotical effect when being used with commonly used antibiotics in- vitro. [Method] Broth microdilution method and checkerboard dilution method were used and the reference strain E. coli ATCC25922 and clinical sample separation strains of E. coli and Salmonella were selected to determine the minimum inhibitory concentration（ MIC） and fractional inhibitory concentration（ FIC）. [Result] The MICs of Berberis julianae Schneid and Cinnamomum camphora（ L.） Presl. for E. coli and Salmonella were both 1 mg /mL,and that of other antibiotics for the two strains were in the quality control range provided by NCCLS. [Conclusion] The synergistic effect or additive effect were observed when they were applied with the commonly used antibiotics.
基金Supported by the National Key Research and Development Plan(2018YFC-06101)Sichuan Provincial Natural Science Foundation(2022NSFSC1605)+2 种基金Traditional Chinese Medicine(Ethnic Medicine)Standard Improvement of Sichuan Medical Products Administration(510201202102305)Leading Talents Support Plan of National Ethnic Affairs Commission in 2022Special Project for the University-Level Innovation Team in the Fundamental Research Funds for the Central Universities(ZYN2022067).
文摘[Objectives] To establish the quality standard of barberry branches. [Methods] Microscopic identification and thin layer identification were used to qualitatively identify barberry branches. Berberine content was determined by HPLC method, and the content of water, total ash, acid insoluble ash and extract was detected according to the method of the Chinese Pharmacopoeia (2020 edition). [Results] The microscopic identification showed that the features were obvious, and stone cells, cork cells, epidermal cells, stomata, fibers and catheter with reticulated pores could be found. Berberine was detected in barberry branches by thin layer chromatography, and the characteristic spots were separated clearly. Moisture, total ash, and acid insoluble ash content shall not exceed 13%, 6%, and 3%, respectively, and extract content shall not be less than 10%. Berberine hydrochloride (C_(20)H_(18)ClNO_(4)) should be calculated in the branches of Berberis wilsonae Hemsley and Berberis aggregata C. K. Schneid., and berberine (C_(20)H_(17)NO_(4)) content should not be less than 0.05%. The linear relationship was good in the range of 0.002-0.240 mg/mL ( R^(2)=0.999 5). The average recovery was 89.63%, and RSD was 5.28%. [Conclusions] The method was simple, accurate and reproducible, and can be used for the quality control of barberry branches.
文摘目的 基于网络药理学和分子对接探讨“寒凉”中药黄连、黄芩、黄柏(三黄)治疗痴呆的作用机制。方法 通过数据挖掘《宣明论方》,筛选出符合“寒凉”属性并且是治疗“痴呆症”的常见中药黄芩、黄连、黄柏。通过中药系统药理学数据库与分析平台(Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform, TCMSP)数据库检索“三黄”的有效成分和作用靶点,在GeneCards、DrugBank等数据库检索痴呆症(dementia)靶点。利用Cytoscape3.7.2构建“药物-成分-靶点”网络,用VENNY2.1获得“三黄”和痴呆症的交集靶点后,构建蛋白质互作网络(protein-protein interaction networks, PPI),利用Metascape库对核心靶点进行GO、KEGG富集分析,并构建“三黄”药物-成分-靶点-通路-疾病网络,最后利用分子对接,以反向验证“三黄”活性成分与核心靶点之间的关系。结果 筛选出“三黄”治疗痴呆症的重要活性成分有槲皮素、β-谷甾醇、小檗碱、黄连碱等,关键核心靶点有TP53、AKT1、IL6、JUN、MAPK14、TNF等。KEGG通路分析有129条,主要有AGE-RAGE信号介导的糖尿病并发症通路、PI3K-Akt信号通路、钙信号通路等;GO功能中,分子功能117条,细胞组成61条,生物过程1 366条,主要涉及配体激活转录因子活性、DNA结合转录因子结合等分子功能,膜微区、突触后膜等细胞组成,活性氧代谢过程、对脂多糖的反应、炎症反应等生物过程。分子对接结果可以看出重要活性成分与核心靶点全部对接成功。结论 本研究结果表明,“三黄”特有的药性优势,可以通过多成分、多靶点、多通路的方式治疗痴呆症,为“三黄”治疗痴呆症临床应用和机制研究提供了依据。
