LOSS OF HETEROZYGOSITY FOR MARKERS ON 22 CHROMOSOME IN SPORADIC SCHWANNOMA

Objective To analyze the loss of heterozygosity (LOH) for markers on chromosew 22 (CHR 22 ) and its significance with their clinical behaviors. Methods The frequency of CHR22 LOH in 36 schwannomas was observed by denatured polyacrylamide gels and silver staining, and the proliferative index of schwannoma wes calculated by Ki-67 and PCNA immunohistochemistry. Results 15 schwannomas (41. 6% ) shawed allele lass. The proliferative index of schwannomas with LOH were significantly higher than those without LOH (P< 0. 05). In acoustic neuromas, patients with LOH were younger at the age of diagnosis, larger size of tumor, shorter history and higher growth rate than those without LOH, but with no significance. Conclusion CHR22 IDH was the frequent event in the tumorigenesis of sporadic schwannoma. There were some links beteen CHR22 LOH and clinical behavior.

散发神经鞘瘤22号染色体杂合子丢失

目的 分析神经鞘瘤 2 2号染色体 (CHR2 2 )杂合子丢失及其与临床行为之间的关系。方法 选择 4个具有高度多态性的微卫星标记物 ,通过变性聚丙烯酰胺凝胶、银染观察 3 6例神经鞘瘤CHR2 2杂合子丢失 (LOH)频率 ;以免疫组织化学方法检测Ki 67、PCNA的增殖指数。结果 3 6例神经鞘瘤中 15例发生CHR2 2杂合子丢失 (41.6% ) ,4个微卫星标记物缺失的频率分别为 19.4% (D2 2S2 68)、2 2 .2 % (D2 2S2 64 )、19.4% (D2 2S2 80 )、16.7% (CRYB2 ) ;发生CHR2 2缺失的神经鞘瘤的增殖指数高于无缺失者 (P <0 .0 5 )。结论 CHR2 2杂合子丢失是神经鞘瘤发生中的常发事件 ,缺失的区域包括NF2基因 ,CHR2 2杂合子丢失与听神经瘤的临床行为有一定的关系 ,CHR2

神经鞘瘤的分子遗传学研究

目的:分析神经鞘瘤22号染色体杂合子丢失(CHR22 LOH)及其与临床行为之间的关系。方法:通过变性聚丙烯酰胺凝胶、银染观察神经鞘瘤CHR22 LOH频率;通过Ki-67、PCNA免疫组织化学计算神经鞘瘤的增殖指数。结果:36例神经鞘瘤中15例发生CHR22 LOH(41.6％),发生缺失的神经鞘瘤的增殖指数高于无缺失者(P<0.05)。结论:CHR22 LOH是神经鞘瘤发生中的常发事件,缺失的区域包括NF2基因,CHR22 LOH可能是神经鞘瘤增殖的重要因素。