Objective:To evaluate antioxidant effects of Scrophularia(S.)striata ethanol extract,trehalose and cysteine added to diluents on cryopreserved goat epididymal sperms.Methods:Motility and standard motion parameters of ...Objective:To evaluate antioxidant effects of Scrophularia(S.)striata ethanol extract,trehalose and cysteine added to diluents on cryopreserved goat epididymal sperms.Methods:Motility and standard motion parameters of sperm were assessed by using computer assisted sperm motility analysis system.Sperm viability was evaluated by eosin-nigrosin staining method.Hypo-osmotic swelling test was used to evaluate membrane health.Thiobarbituric acid testing was used to measure malondialdehyde(MDA)concentrations.To assess DNA fragmentation,sperm chromatin dispersion test was used.In Experiment 1,treatments consisting of basal Tris diluent supplemented with 25,50 or 100µg/mL of S.striata ethanol extract gave the best concentration to the freezing diluents.Experiment 2 was carried out to compare the best concentration of S.striata ethanol extract(50µg/mL)resulting from the first experiment with 150 mM trehalose and/or 5 mM cysteine alone or in combination.Results:S.striata ethanol extract(50µg/mL)significantly increased sperm viability,motility and progressive motility and at the same time decreased MDA concentration and DNA fragmentation compared to other treatments(P<0.05).In addition,all treatment groups resulted in viability,membrane health,total motility,progressive motility,curvilinear velocity,straightline velocity higher and MDA lower compared to the control group(P<0.05).Acrosome integrity was significantly higher in 50µg/mL of S.striata ethanol extract combined with cysteine,trehalose,or cysteine+trehalose groups than those in the control,trehalose,cysteine,and 50µg/mL of S.striata ethanol extract groups(P<0.05).Regarding DNA,extenders supplemented with 50µg/mL of S.striata ethanol extract,50µg/mL of S.striata ethanol extract+trehalose,and 50µg/mL of S.striata ethanol extract+trehalose+cysteine were superior to other treatments.Conclusions:Adding 50µg/mL of S.striata ethanol extract alone or in combination with trehalose and cysteine can improve the quality of cryopreserved epididymal sperms of goats.展开更多
An oligosaccharide homologue named SnS-2 was isolated from the root of Scrophularia ningpoensis Hemsl. SnS-2 was purified by means of gel-permeation chromatography and ion-exchange chromatography. Its physico-chemical...An oligosaccharide homologue named SnS-2 was isolated from the root of Scrophularia ningpoensis Hemsl. SnS-2 was purified by means of gel-permeation chromatography and ion-exchange chromatography. Its physico-chemical properties, including carbohydrate content and molecular weight were determined. The structure of SnS-2 was elucidated by chemical methods along with 1H and 13C NMR spectroscopy, including two-dimensional DQCOSY and H-detected 1H, 13C HMQC experiments. These results show that SnS-2 possesses a backbone con-sisting of terminal a-Galp-(1, a-Galp-(16), a-Glcp-(16) and nonreducing end -Fruf-(2. The bioactive as-say showed that it could inhibit the growth of Lewis pulmonary carcinoma implanted in mice.展开更多
Two new iridoid glycosides,named scrophularianoids A（1） and B（2）,were isolated from the roots of Scrophularia ningpoensis.The chemical structures were established on the basis of extensive analyses of spectroscopi...Two new iridoid glycosides,named scrophularianoids A（1） and B（2）,were isolated from the roots of Scrophularia ningpoensis.The chemical structures were established on the basis of extensive analyses of spectroscopic data.Compounds 1 and 2 were inactive in our preliminary in vitro myocardial protective bioassay.展开更多
Scrophularia ningpoensis has long been used in the Chinese Materia Medica for inflammation.Like other herbal medicines,S.ningpoensis collected from different localities may considerably differ in their therapeutic eff...Scrophularia ningpoensis has long been used in the Chinese Materia Medica for inflammation.Like other herbal medicines,S.ningpoensis collected from different localities may considerably differ in their therapeutic efficacy,and the one grown in Zhejiang Province is recognized as geo-authentic.However,it is difficult to confirm the geo-graphical authenticity by similar morphological characteristics.In the present study,inter-simple sequence repeat(ISSR) markers were conducted to detect S.ningpoensis from different origins.A 1 259-bp fragment amplified by primer UBC874 was found only in geo-authentic ones.By cloning and sequencing that specific band,sequence characterized amplified region(SCAR) markers were designed to distinguish geo-authentic S.ningpoensis from others.This is a rapid and easy method that can be used to identify the geographical authenticity of S.ningpoensis.展开更多
AIM: To analyze the major constituents in Radix Scrophulariae(Scrophularia ningpoensis). METHOD: Radix Scrophulariae was analyzed by HPLC coupled with electrospray ionization quadrupole time-of-flight tandem mass spec...AIM: To analyze the major constituents in Radix Scrophulariae(Scrophularia ningpoensis). METHOD: Radix Scrophulariae was analyzed by HPLC coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry(ESI-Q-TOF MS/MS). Compounds were separated by HPLC using a C18 column and gradient elution of acetonitrile and 0.1 %(V/V) acetic acid-water. Negative ion mode was employed. RESULTS: A total of thirty-six compounds, including fourteen iridoid glycosides, nineteen phenylpropanoid glycosides, and three organic acids, were identified from Radix Scrophulariae based on the accurate mass measurement of precursor and product ions. Twenty-one of the constituents were identified by comparing their retention times(tR) and ESI-MS/MS data with those of reference standards and/or previous publications, while another fifteen compounds were tentatively identified or deduced according to their Q-TOF MS/MS data which afforded sufficient structural information. CONCLUSION: It is believed that this study is useful for the identification of constituents in Radix Scrophulariae, as well as related plants and complex prescriptions.展开更多
文摘Objective:To evaluate antioxidant effects of Scrophularia(S.)striata ethanol extract,trehalose and cysteine added to diluents on cryopreserved goat epididymal sperms.Methods:Motility and standard motion parameters of sperm were assessed by using computer assisted sperm motility analysis system.Sperm viability was evaluated by eosin-nigrosin staining method.Hypo-osmotic swelling test was used to evaluate membrane health.Thiobarbituric acid testing was used to measure malondialdehyde(MDA)concentrations.To assess DNA fragmentation,sperm chromatin dispersion test was used.In Experiment 1,treatments consisting of basal Tris diluent supplemented with 25,50 or 100µg/mL of S.striata ethanol extract gave the best concentration to the freezing diluents.Experiment 2 was carried out to compare the best concentration of S.striata ethanol extract(50µg/mL)resulting from the first experiment with 150 mM trehalose and/or 5 mM cysteine alone or in combination.Results:S.striata ethanol extract(50µg/mL)significantly increased sperm viability,motility and progressive motility and at the same time decreased MDA concentration and DNA fragmentation compared to other treatments(P<0.05).In addition,all treatment groups resulted in viability,membrane health,total motility,progressive motility,curvilinear velocity,straightline velocity higher and MDA lower compared to the control group(P<0.05).Acrosome integrity was significantly higher in 50µg/mL of S.striata ethanol extract combined with cysteine,trehalose,or cysteine+trehalose groups than those in the control,trehalose,cysteine,and 50µg/mL of S.striata ethanol extract groups(P<0.05).Regarding DNA,extenders supplemented with 50µg/mL of S.striata ethanol extract,50µg/mL of S.striata ethanol extract+trehalose,and 50µg/mL of S.striata ethanol extract+trehalose+cysteine were superior to other treatments.Conclusions:Adding 50µg/mL of S.striata ethanol extract alone or in combination with trehalose and cysteine can improve the quality of cryopreserved epididymal sperms of goats.
文摘An oligosaccharide homologue named SnS-2 was isolated from the root of Scrophularia ningpoensis Hemsl. SnS-2 was purified by means of gel-permeation chromatography and ion-exchange chromatography. Its physico-chemical properties, including carbohydrate content and molecular weight were determined. The structure of SnS-2 was elucidated by chemical methods along with 1H and 13C NMR spectroscopy, including two-dimensional DQCOSY and H-detected 1H, 13C HMQC experiments. These results show that SnS-2 possesses a backbone con-sisting of terminal a-Galp-(1, a-Galp-(16), a-Glcp-(16) and nonreducing end -Fruf-(2. The bioactive as-say showed that it could inhibit the growth of Lewis pulmonary carcinoma implanted in mice.
文摘Two new iridoid glycosides,named scrophularianoids A（1） and B（2）,were isolated from the roots of Scrophularia ningpoensis.The chemical structures were established on the basis of extensive analyses of spectroscopic data.Compounds 1 and 2 were inactive in our preliminary in vitro myocardial protective bioassay.
基金Project supported by the National Basic Research Program (973) of China(No.2007CB411600)the National Natural Science Foundation of China(No.31070205)the Key Agricultural Program of Pan’an County of Zhejiang Province,China(No.2005ZB01)
文摘Scrophularia ningpoensis has long been used in the Chinese Materia Medica for inflammation.Like other herbal medicines,S.ningpoensis collected from different localities may considerably differ in their therapeutic efficacy,and the one grown in Zhejiang Province is recognized as geo-authentic.However,it is difficult to confirm the geo-graphical authenticity by similar morphological characteristics.In the present study,inter-simple sequence repeat(ISSR) markers were conducted to detect S.ningpoensis from different origins.A 1 259-bp fragment amplified by primer UBC874 was found only in geo-authentic ones.By cloning and sequencing that specific band,sequence characterized amplified region(SCAR) markers were designed to distinguish geo-authentic S.ningpoensis from others.This is a rapid and easy method that can be used to identify the geographical authenticity of S.ningpoensis.
基金supported by the National Natural Science Foundation of China(Nos.30901956,30973965)2011’Program for Excellent Scientific and Technological Innovation Team of Jiangsu Higher Education and the Priority Academic Program Development of Jiangsu Higher Education Institutions
文摘AIM: To analyze the major constituents in Radix Scrophulariae(Scrophularia ningpoensis). METHOD: Radix Scrophulariae was analyzed by HPLC coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry(ESI-Q-TOF MS/MS). Compounds were separated by HPLC using a C18 column and gradient elution of acetonitrile and 0.1 %(V/V) acetic acid-water. Negative ion mode was employed. RESULTS: A total of thirty-six compounds, including fourteen iridoid glycosides, nineteen phenylpropanoid glycosides, and three organic acids, were identified from Radix Scrophulariae based on the accurate mass measurement of precursor and product ions. Twenty-one of the constituents were identified by comparing their retention times(tR) and ESI-MS/MS data with those of reference standards and/or previous publications, while another fifteen compounds were tentatively identified or deduced according to their Q-TOF MS/MS data which afforded sufficient structural information. CONCLUSION: It is believed that this study is useful for the identification of constituents in Radix Scrophulariae, as well as related plants and complex prescriptions.
