AIM:To investigate the effect of Scutellariae Radix extract(SRE)on ulcerative colitis(UC)in rats induced by dextran-sulfate sodium(DSS).METHODS:Colitis was induced in male Sprague-Dawley(SD)rats(170-180 g)by 4% dextra...AIM:To investigate the effect of Scutellariae Radix extract(SRE)on ulcerative colitis(UC)in rats induced by dextran-sulfate sodium(DSS).METHODS:Colitis was induced in male Sprague-Dawley(SD)rats(170-180 g)by 4% dextran sulfate sodium(DSS,wt/v;MW 54 000)in drinking water for 8 d.The treated rats received 4% DSS and SRE orally(100 mg/kg per day).Control rats received either tap water or SRE only.Macroscopic assessment which included body weight changes,fecal occult blood and stool consistency were determined daily.At the appointed time,the rats were sacrificed and the entire colons were removed.The colon length and the myeloperoxidase(MPO)activity were measured.The severity of colitis was graded by morphological and histological assessments.The ion transport activity of the colonic mucosa was assessed by electrophysiological technique.RESULTS:Rats treated with oral administration of 4% DSS regularly developed clinical and macroscopic signs of colitis.Treatment with SRE relieved the symptoms,including the reduction in body weight,shortening and ulceration of the colon.Administration of SRE also significantly reduced the histological damage induced by DSS.Moreover,the ISC responses of the colonic mucosato forskolin were suppressed after the induction of colitis.The stimulated ion transport activity of DSS-rats treated with SRE displayed significant improvement in the secretory responsiveness.CONCLUSION:SRE was effective in treating acute DSS-induced ulcerative colitis,as gauged by reduced clinical disease,improved macroscopic and histological damage scores,and enhanced recovery of normal colonic secretory function.展开更多
Breast cancer(BCa)is one of the most common malignant tumors that seriously affect women’s physical and mental health and even endanger their lives.Establishing a primary prevention program for BCa remains a challeng...Breast cancer(BCa)is one of the most common malignant tumors that seriously affect women’s physical and mental health and even endanger their lives.Establishing a primary prevention program for BCa remains a challenge.Traditional Chinese medicine,Scutellariae Radix(SR),has been used to treat BCa while its pharmacological mechanism is still unclear.This study aims to reveal the pharmacological mechanism of SR in the treatment of BCa.Chemical constituents of SR were obtained and the targets of the compound were identified via Traditional Chinese Medicine Systems Pharmacology Database(TCMSP).GeneCards and DrugBank databases were used to collect BCa’s related target genes.Disease-compound common target protein interaction network was established by using the STRING database.The network diagrams of the active component-action target and protein-protein interaction(PPI)networks were constructed by Cytoscape software.Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analyses were carried out to further explore the BCa mechanism and therapeutic effect of SR.As a result,121 active compounds were obtained.The Venn diagram results showed that SR and BCa had 57 intersection targets,the more frequently targeted ones are TP53,JUN,CCND1,AKT1,which could affect biological processes such as response to steroid hormone and positive regulation of cell death.KEGG analysis revealed that these targets were related to IL-17 signaling pathway,p53 signaling pathway and PI3K-Akt signaling pathway.In conclusion,this study preliminarily verified the target and mode of action of SR in the treatment of BCa,laying a foundation for further research on its mechanism.展开更多
目的:基于网络药理学探讨“黄芪-白术-茯苓-当归”组方治疗糖尿病肾病(diabetic kidney disease,DKD)的作用机制。方法:通过中药系统药理学数据库与分析平台(Traditional Chinese Medicine Systems Pharmacology Database and Analysis ...目的:基于网络药理学探讨“黄芪-白术-茯苓-当归”组方治疗糖尿病肾病(diabetic kidney disease,DKD)的作用机制。方法:通过中药系统药理学数据库与分析平台(Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform,TCMSP)检索黄芪、白术、茯苓、当归的化学成分和作用靶点。借助OMIM数据库检索DKD的靶点,进而获取药物与疾病的交集靶点。采用Cytoscape 3.7.2软件绘制“药物-活性成分-靶点”网络图,并筛选关键成分。利用STRING数据库构建蛋白质-蛋白质相互作用(protein-protein interaction,PPI)网络,并筛选核心靶点。通过Metascape数据库对交集靶点进行基因本体(gene ontology,GO)和京都基因与基因组百科全书(Kyoto encyclopedia of genes and genomes,KEGG)分析。结果:“黄芪-白术-茯苓-当归”组方的活性成分44种,其中黄芪20种,白术7种,茯苓15种,当归2种。黄芪靶点413个,白术靶点20个,茯苓靶点26个,当归靶点64个。DKD靶点1024个,疾病与药物的交集靶点19个。槲皮素、刺芒柄花素、山柰酚、异鼠李素可能是关键成分。白细胞介素(interleukin)-6、IL-10、IL-1A、IL-1β、信号传导与转录激活因子1(signal transducer and activator of transcription 1,STAT1)、C-反应蛋白(C-reactive protein,CRP)等可能是核心靶点。GO分析主要涉及血管生成调节、活性氧代谢过程、细胞因子受体结合等。KEGG通路主要包括糖尿病并发症中的AGE-RAGE信号通路、MAPK信号通路、Th17细胞分化、JAK-STAT信号通路等。结论:“黄芪-白术-茯苓-当归”组方可能通过槲皮素、刺芒柄花素等活性成分,调控AGE-RAGE、MAPK等信号通路,作用于IL-6、IL-1A、IL-1β、STAT1、CRP等靶点,从而发挥治疗DKD的作用。展开更多
基金a direct grant for research from The ChineseUniversity of Hong Kong,No. 2041075 awarded to Wing-HungKo
文摘AIM:To investigate the effect of Scutellariae Radix extract(SRE)on ulcerative colitis(UC)in rats induced by dextran-sulfate sodium(DSS).METHODS:Colitis was induced in male Sprague-Dawley(SD)rats(170-180 g)by 4% dextran sulfate sodium(DSS,wt/v;MW 54 000)in drinking water for 8 d.The treated rats received 4% DSS and SRE orally(100 mg/kg per day).Control rats received either tap water or SRE only.Macroscopic assessment which included body weight changes,fecal occult blood and stool consistency were determined daily.At the appointed time,the rats were sacrificed and the entire colons were removed.The colon length and the myeloperoxidase(MPO)activity were measured.The severity of colitis was graded by morphological and histological assessments.The ion transport activity of the colonic mucosa was assessed by electrophysiological technique.RESULTS:Rats treated with oral administration of 4% DSS regularly developed clinical and macroscopic signs of colitis.Treatment with SRE relieved the symptoms,including the reduction in body weight,shortening and ulceration of the colon.Administration of SRE also significantly reduced the histological damage induced by DSS.Moreover,the ISC responses of the colonic mucosato forskolin were suppressed after the induction of colitis.The stimulated ion transport activity of DSS-rats treated with SRE displayed significant improvement in the secretory responsiveness.CONCLUSION:SRE was effective in treating acute DSS-induced ulcerative colitis,as gauged by reduced clinical disease,improved macroscopic and histological damage scores,and enhanced recovery of normal colonic secretory function.
文摘Breast cancer(BCa)is one of the most common malignant tumors that seriously affect women’s physical and mental health and even endanger their lives.Establishing a primary prevention program for BCa remains a challenge.Traditional Chinese medicine,Scutellariae Radix(SR),has been used to treat BCa while its pharmacological mechanism is still unclear.This study aims to reveal the pharmacological mechanism of SR in the treatment of BCa.Chemical constituents of SR were obtained and the targets of the compound were identified via Traditional Chinese Medicine Systems Pharmacology Database(TCMSP).GeneCards and DrugBank databases were used to collect BCa’s related target genes.Disease-compound common target protein interaction network was established by using the STRING database.The network diagrams of the active component-action target and protein-protein interaction(PPI)networks were constructed by Cytoscape software.Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analyses were carried out to further explore the BCa mechanism and therapeutic effect of SR.As a result,121 active compounds were obtained.The Venn diagram results showed that SR and BCa had 57 intersection targets,the more frequently targeted ones are TP53,JUN,CCND1,AKT1,which could affect biological processes such as response to steroid hormone and positive regulation of cell death.KEGG analysis revealed that these targets were related to IL-17 signaling pathway,p53 signaling pathway and PI3K-Akt signaling pathway.In conclusion,this study preliminarily verified the target and mode of action of SR in the treatment of BCa,laying a foundation for further research on its mechanism.
文摘目的:基于网络药理学探讨“黄芪-白术-茯苓-当归”组方治疗糖尿病肾病(diabetic kidney disease,DKD)的作用机制。方法:通过中药系统药理学数据库与分析平台(Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform,TCMSP)检索黄芪、白术、茯苓、当归的化学成分和作用靶点。借助OMIM数据库检索DKD的靶点,进而获取药物与疾病的交集靶点。采用Cytoscape 3.7.2软件绘制“药物-活性成分-靶点”网络图,并筛选关键成分。利用STRING数据库构建蛋白质-蛋白质相互作用(protein-protein interaction,PPI)网络,并筛选核心靶点。通过Metascape数据库对交集靶点进行基因本体(gene ontology,GO)和京都基因与基因组百科全书(Kyoto encyclopedia of genes and genomes,KEGG)分析。结果:“黄芪-白术-茯苓-当归”组方的活性成分44种,其中黄芪20种,白术7种,茯苓15种,当归2种。黄芪靶点413个,白术靶点20个,茯苓靶点26个,当归靶点64个。DKD靶点1024个,疾病与药物的交集靶点19个。槲皮素、刺芒柄花素、山柰酚、异鼠李素可能是关键成分。白细胞介素(interleukin)-6、IL-10、IL-1A、IL-1β、信号传导与转录激活因子1(signal transducer and activator of transcription 1,STAT1)、C-反应蛋白(C-reactive protein,CRP)等可能是核心靶点。GO分析主要涉及血管生成调节、活性氧代谢过程、细胞因子受体结合等。KEGG通路主要包括糖尿病并发症中的AGE-RAGE信号通路、MAPK信号通路、Th17细胞分化、JAK-STAT信号通路等。结论:“黄芪-白术-茯苓-当归”组方可能通过槲皮素、刺芒柄花素等活性成分,调控AGE-RAGE、MAPK等信号通路,作用于IL-6、IL-1A、IL-1β、STAT1、CRP等靶点,从而发挥治疗DKD的作用。
