从芝麻中提取总RNA,用反转录聚合酶链式反应得到芝麻蛋白Ses i 3基因,构建pET-22b(+)质粒表达载体,转入BL21(DE3)感受态细胞宿主表达菌中诱导表达,经镍离子亲和层析柱获得纯品目的蛋白。十二烷基硫酸钠-聚丙烯酰胺凝胶电泳结果显示目的...从芝麻中提取总RNA,用反转录聚合酶链式反应得到芝麻蛋白Ses i 3基因,构建pET-22b(+)质粒表达载体,转入BL21(DE3)感受态细胞宿主表达菌中诱导表达,经镍离子亲和层析柱获得纯品目的蛋白。十二烷基硫酸钠-聚丙烯酰胺凝胶电泳结果显示目的蛋白分子质量约为66 kDa。进而采用BALB/c小鼠过敏模型评价重组Ses i 3蛋白与天然Ses i 3蛋白诱发小鼠芝麻过敏反应的差别,通过测定相关过敏性指标(特异性抗体羊抗鼠免疫球蛋白(immunoglobulin,Ig)E、IgG1和IgG2a;细胞因子白细胞介素(interleukin,IL)-4、IL-5、干扰素-γ和组胺)发现,重组蛋白Ses i 3诱发小鼠芝麻过敏的能力与天然Ses i 3蛋白相似。因此,本研究通过原核表达系统获得重组的芝麻重组蛋白Ses i 3,并证实其与天然Ses i 3蛋白具有相似的免疫活性,可以用于后续的芝麻致敏性研究。展开更多
As a thin film solar cell absorber material, antimony selenide (Sb<sub>2</sub>Se<sub>3</sub>) has become a potential candidate recently because of its unique optical and electrical properties a...As a thin film solar cell absorber material, antimony selenide (Sb<sub>2</sub>Se<sub>3</sub>) has become a potential candidate recently because of its unique optical and electrical properties and easy fabrication method. X-ray photoelectron spectroscopy (XPS) was used to determine the stoichiometry and composition of electroless Sb<sub>2</sub>Se<sub>3</sub> thin films using depth profile studies. The surface layers were analyzed nearly stoichiometric. But the abundant amount of antimony makes the inner layer electrically more conductive.展开更多
为预测芝麻过敏原Ses i 3的B细胞线性抗原表位和三级结构,使用SOPMA、DNAStar和The BepiPred1.0 Server等生物信息学软件,采用多种方案对Ses i 3抗原性指数、亲水性、表面可及性、柔韧性等参数及其二级结构进行预测,对预测抗原表位综合...为预测芝麻过敏原Ses i 3的B细胞线性抗原表位和三级结构,使用SOPMA、DNAStar和The BepiPred1.0 Server等生物信息学软件,采用多种方案对Ses i 3抗原性指数、亲水性、表面可及性、柔韧性等参数及其二级结构进行预测,对预测抗原表位综合分析。利用SWISS-MODEL程序进行同源建模并用Ramachandran软件评价结构稳定性。结果:序列SESKDP,RQKHQGEHG,NRKSP,QHG,YQREKGRQDDDNPTDPEKQY,RRQG,KYREQQGREGGRGE,EGR,EQGR,QHG,RQDR,ENP,RHE,ESK,RPTH,ASQ,SRSRGSYQGETRGRP,ANNNE,SRSQQ,GPRQQQQGR最可能是Ses i 3蛋白的B细胞线性抗原表位。以5e1r.2为模板,同源建模的方式成功构建了芝麻过敏原Ses i 3蛋白的三级结构,并经Ramachandran评价显示蛋白模型构象稳定。此结果为制备特异性抗体肽段及过敏原检测等提供了依据。展开更多
To compare the clinical and pathological manifestations of patients with antineutrophil cytoplasmic autoantibodies (ANCA) directed against proteinas e 3 (anti PR3) or myeloperoxidase (anti MPO). Methods. One hundred a...To compare the clinical and pathological manifestations of patients with antineutrophil cytoplasmic autoantibodies (ANCA) directed against proteinas e 3 (anti PR3) or myeloperoxidase (anti MPO). Methods. One hundred and forty patients with ANCA were detected for anti PR3 a nd anti MPO by ELISA. The clinical features at presentation, histopathological characteristics and outcome of all patients who were tested positive for anti P R3 or anti MPO were analysed.Results. In anti PR3 group (n=21), 16 cases (76.2%) had systemic vasculitis , in which Wegener’s granulomatosis prevailed (13 cases, 61.9%). In anti MPO g roup (n=31), 19 cases (61.3%) were diagnosed as systemic vasculitis and 12 case s (38.7%) as microscopic angiitis. For vasculitic patients with anti PR3 and a nti MPO, the disease duration at diagnosis was 9.6±2.0m and 4.4±0.9m respecti vely, P< 0.05;vasculitis activity index (BVAS) and mean number of affected organ were 22.5±2.1, 5.0±0.4 and 25.1±1.7, 4.8±0.4 respectively, P >0.05;upper r espiratory tract, eye and joint involvements were 11(68.8%), 7(43.8%), 11(68.8 %) and 7(36.8%), 2(10.5%), 5(26.3%) respectively, P< 0.05.Although there was no statistical difference in renal involvement between these two groups, patien ts with serum creatine >500 μmol/L were more commonly seen in anti MPO group t han in anti PR3 group, which were 8(42.1%) and 2(12.5%) respectively, P< 0.05 . Ten relapses were seen in anti PR3 group and only 2 in anti MPO group, but t he acute mortality rate in anti MPO group (5/19, 27.4%) was much higher than t hat in anti PR3 group (1/16, 6.3%). Conclusions. Anti PR3 and anti MPO occurred mainly in systemic vasculitis. A large divergence was seen in the disease spectrum between patients with anti PR 3 and those with anti MPO. In particular, upper respiratory tract, eye and join t involvements, granuloma formation and relapse were more prominent in anti PR3 patients. By contrast, the anti MPO patients had a more acute disease onset, m ore rapid progressive renal involvement and a higher acute mortality rate.展开更多
文摘从芝麻中提取总RNA,用反转录聚合酶链式反应得到芝麻蛋白Ses i 3基因,构建pET-22b(+)质粒表达载体,转入BL21(DE3)感受态细胞宿主表达菌中诱导表达,经镍离子亲和层析柱获得纯品目的蛋白。十二烷基硫酸钠-聚丙烯酰胺凝胶电泳结果显示目的蛋白分子质量约为66 kDa。进而采用BALB/c小鼠过敏模型评价重组Ses i 3蛋白与天然Ses i 3蛋白诱发小鼠芝麻过敏反应的差别,通过测定相关过敏性指标(特异性抗体羊抗鼠免疫球蛋白(immunoglobulin,Ig)E、IgG1和IgG2a;细胞因子白细胞介素(interleukin,IL)-4、IL-5、干扰素-γ和组胺)发现,重组蛋白Ses i 3诱发小鼠芝麻过敏的能力与天然Ses i 3蛋白相似。因此,本研究通过原核表达系统获得重组的芝麻重组蛋白Ses i 3,并证实其与天然Ses i 3蛋白具有相似的免疫活性,可以用于后续的芝麻致敏性研究。
文摘As a thin film solar cell absorber material, antimony selenide (Sb<sub>2</sub>Se<sub>3</sub>) has become a potential candidate recently because of its unique optical and electrical properties and easy fabrication method. X-ray photoelectron spectroscopy (XPS) was used to determine the stoichiometry and composition of electroless Sb<sub>2</sub>Se<sub>3</sub> thin films using depth profile studies. The surface layers were analyzed nearly stoichiometric. But the abundant amount of antimony makes the inner layer electrically more conductive.
文摘为预测芝麻过敏原Ses i 3的B细胞线性抗原表位和三级结构,使用SOPMA、DNAStar和The BepiPred1.0 Server等生物信息学软件,采用多种方案对Ses i 3抗原性指数、亲水性、表面可及性、柔韧性等参数及其二级结构进行预测,对预测抗原表位综合分析。利用SWISS-MODEL程序进行同源建模并用Ramachandran软件评价结构稳定性。结果:序列SESKDP,RQKHQGEHG,NRKSP,QHG,YQREKGRQDDDNPTDPEKQY,RRQG,KYREQQGREGGRGE,EGR,EQGR,QHG,RQDR,ENP,RHE,ESK,RPTH,ASQ,SRSRGSYQGETRGRP,ANNNE,SRSQQ,GPRQQQQGR最可能是Ses i 3蛋白的B细胞线性抗原表位。以5e1r.2为模板,同源建模的方式成功构建了芝麻过敏原Ses i 3蛋白的三级结构,并经Ramachandran评价显示蛋白模型构象稳定。此结果为制备特异性抗体肽段及过敏原检测等提供了依据。
文摘To compare the clinical and pathological manifestations of patients with antineutrophil cytoplasmic autoantibodies (ANCA) directed against proteinas e 3 (anti PR3) or myeloperoxidase (anti MPO). Methods. One hundred and forty patients with ANCA were detected for anti PR3 a nd anti MPO by ELISA. The clinical features at presentation, histopathological characteristics and outcome of all patients who were tested positive for anti P R3 or anti MPO were analysed.Results. In anti PR3 group (n=21), 16 cases (76.2%) had systemic vasculitis , in which Wegener’s granulomatosis prevailed (13 cases, 61.9%). In anti MPO g roup (n=31), 19 cases (61.3%) were diagnosed as systemic vasculitis and 12 case s (38.7%) as microscopic angiitis. For vasculitic patients with anti PR3 and a nti MPO, the disease duration at diagnosis was 9.6±2.0m and 4.4±0.9m respecti vely, P< 0.05;vasculitis activity index (BVAS) and mean number of affected organ were 22.5±2.1, 5.0±0.4 and 25.1±1.7, 4.8±0.4 respectively, P >0.05;upper r espiratory tract, eye and joint involvements were 11(68.8%), 7(43.8%), 11(68.8 %) and 7(36.8%), 2(10.5%), 5(26.3%) respectively, P< 0.05.Although there was no statistical difference in renal involvement between these two groups, patien ts with serum creatine >500 μmol/L were more commonly seen in anti MPO group t han in anti PR3 group, which were 8(42.1%) and 2(12.5%) respectively, P< 0.05 . Ten relapses were seen in anti PR3 group and only 2 in anti MPO group, but t he acute mortality rate in anti MPO group (5/19, 27.4%) was much higher than t hat in anti PR3 group (1/16, 6.3%). Conclusions. Anti PR3 and anti MPO occurred mainly in systemic vasculitis. A large divergence was seen in the disease spectrum between patients with anti PR 3 and those with anti MPO. In particular, upper respiratory tract, eye and join t involvements, granuloma formation and relapse were more prominent in anti PR3 patients. By contrast, the anti MPO patients had a more acute disease onset, m ore rapid progressive renal involvement and a higher acute mortality rate.
