Recent studies on the inhibition of tumor growth by Se-containing polysaccharide were reviewed.Meanwhile,the possible molecular mechanisms of the inhibition of tumor cell growth through antioxidation,induction of tumo...Recent studies on the inhibition of tumor growth by Se-containing polysaccharide were reviewed.Meanwhile,the possible molecular mechanisms of the inhibition of tumor cell growth through antioxidation,induction of tumor cell apoptosis,blockade of cell cycle,and enhancement of immunity by Se-containing polysaccharide were proposed.In the end,the potential application of Se-containing polysaccharide in the prevention and treatment of tumor was elucidated.展开更多
Two selenium(Se)-containing peptides from Se-enriched rice,TSeMMM and SeMDPGQQ,possess neuroprotective potency against lead(Pb2+)-induced cytotoxicity.However,the crosstalk between mRNA and microRNAs(miRNA)involved in...Two selenium(Se)-containing peptides from Se-enriched rice,TSeMMM and SeMDPGQQ,possess neuroprotective potency against lead(Pb2+)-induced cytotoxicity.However,the crosstalk between mRNA and microRNAs(miRNA)involved in the neuroprotection mechanism remains to be elucidated.In this study,RNA-sequencing and miRNA-sequencing were used to independently identify differentially expressed mRNAs and small RNAs profiles in Pb^(2+)-treated primary fetal rat cortical neurons and then the correlated miRNA-mRNA target pairs were obtained.It was found that 34 mRNAs related to oxidative phosphorylation could be reversed by pretreatment of TSeMMM and SeMDPGQQ.The protective effect of TSeMMM and SeMDPGQQ was mediated by upregulation of miR-107-3p,which downregulates the ATPase H+transporting V0 subunit e1(Atp6v0e1)mRNA level.A zebrafish model was applied to verify the relevance between the targeted mRNA and miRNA by real-time quantitative PCR.The results indicated that miR-107-3p was a potential therapeutic target to achieve neuroprotection of Se-containing peptides via stimulation of Atp6v0e1.展开更多
Altemaria alternata were isolated from wheat seeds collected from villages in Tianshui area of Gansu Province where Kaschin-Beck disease is prevalent. TLC, IR and UV analyses of metabolic extract of A. alternata show ...Altemaria alternata were isolated from wheat seeds collected from villages in Tianshui area of Gansu Province where Kaschin-Beck disease is prevalent. TLC, IR and UV analyses of metabolic extract of A. alternata show that A. alternata produce main secondary metabolites, including alternariol, alternariol methyl ether, and tenuazonic acid.There was a significant decrease in Se-GSH-Px and SOD activities, and an acceleration in lipid peroxidation in rats fed with a diet containing metabolites produced by A. alternata.展开更多
基金supported by the National Natural Science Foundation of China(Grant No.30770276).
文摘Recent studies on the inhibition of tumor growth by Se-containing polysaccharide were reviewed.Meanwhile,the possible molecular mechanisms of the inhibition of tumor cell growth through antioxidation,induction of tumor cell apoptosis,blockade of cell cycle,and enhancement of immunity by Se-containing polysaccharide were proposed.In the end,the potential application of Se-containing polysaccharide in the prevention and treatment of tumor was elucidated.
基金supported by the National Natural Science Foundation of China(32272319,31972020,32202032)the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD).
文摘Two selenium(Se)-containing peptides from Se-enriched rice,TSeMMM and SeMDPGQQ,possess neuroprotective potency against lead(Pb2+)-induced cytotoxicity.However,the crosstalk between mRNA and microRNAs(miRNA)involved in the neuroprotection mechanism remains to be elucidated.In this study,RNA-sequencing and miRNA-sequencing were used to independently identify differentially expressed mRNAs and small RNAs profiles in Pb^(2+)-treated primary fetal rat cortical neurons and then the correlated miRNA-mRNA target pairs were obtained.It was found that 34 mRNAs related to oxidative phosphorylation could be reversed by pretreatment of TSeMMM and SeMDPGQQ.The protective effect of TSeMMM and SeMDPGQQ was mediated by upregulation of miR-107-3p,which downregulates the ATPase H+transporting V0 subunit e1(Atp6v0e1)mRNA level.A zebrafish model was applied to verify the relevance between the targeted mRNA and miRNA by real-time quantitative PCR.The results indicated that miR-107-3p was a potential therapeutic target to achieve neuroprotection of Se-containing peptides via stimulation of Atp6v0e1.
文摘Altemaria alternata were isolated from wheat seeds collected from villages in Tianshui area of Gansu Province where Kaschin-Beck disease is prevalent. TLC, IR and UV analyses of metabolic extract of A. alternata show that A. alternata produce main secondary metabolites, including alternariol, alternariol methyl ether, and tenuazonic acid.There was a significant decrease in Se-GSH-Px and SOD activities, and an acceleration in lipid peroxidation in rats fed with a diet containing metabolites produced by A. alternata.
