Robust Deep Learning Model for Black Fungus Detection Based on Gabor Filter and Transfer Learning

Black fungus is a rare and dangerous mycology that usually affects the brain and lungs and could be life-threatening in diabetic cases.Recently,some COVID-19 survivors,especially those with co-morbid diseases,have been susceptible to black fungus.Therefore,recovered COVID-19 patients should seek medical support when they notice mucormycosis symptoms.This paper proposes a novel ensemble deep-learning model that includes three pre-trained models:reset(50),VGG(19),and Inception.Our approach is medically intuitive and efficient compared to the traditional deep learning models.An image dataset was aggregated from various resources and divided into two classes:a black fungus class and a skin infection class.To the best of our knowledge,our study is the first that is concerned with building black fungus detection models based on deep learning algorithms.The proposed approach can significantly improve the performance of the classification task and increase the generalization ability of such a binary classification task.According to the reported results,it has empirically achieved a sensitivity value of 0.9907,a specificity value of 0.9938,a precision value of 0.9938,and a negative predictive value of 0.9907.

Cultivation of Black Fungus under Forest in Middle-high Altitude Areas

[Objective] The study was to solve the contradiction between fungus and forest during the production of black fungi, and to obtain relatively high economic benefits and ecological benefits. [Method] Black fungi were cultivated in forest with middle-high altitude of 500-1 600 m. The germination time of fungus stick, germination days of buds, growth days of fruiting body, commodity exterior, marketing effect and biological efficiency of black fungi were investigated. [Result] Compared with traditional cultivation, black fungus products cultivated under forest had the taste and flavor of wild black fungus. Fungus chaff wastage was directly decomposed by the roots in forest land, improving the soil structure of forest land. The need of moisture retention and the frequent access of administrative staff reduced the probability of forest fire occurrence. [Conclusion] Cultivation of black fungus under forest saves more than 70% fixed-asset investment on fungus shed, and the biological efficiency reached 12.2%.

Smart control system for the precision cultivation of black fungus

Black fungus,with high nutritional and medicinal value,has been cultivated in China for a long time,and Heilongjiang alone accounts for about 40%of the global output.At present,the cultivation of black fungus derives mainly from the inheritance of relatively primitive practices and experience of farmers,resulting in inconsistent quality of fungus.In this study,a smart control system for the precision cultivation of black fungus was designed by using intelligent detection and control technology.The system includes a precision culture test environment and remote control system.The precision cultivation environment contains four sub-independent environments.The key parameters such as temperature,humidity,and light behavior were collected and can be adjusted individually,according to the precision cultivation stages.The intelligent remote control system included a controller cabinet,sensors unit,temperature control unit,humidity control unit,light control unit,and information transmitting unit.The controller cabinet includes a key controller which can auto-control the temperature,humidity,and lightly adjust components according to the precision cultivation conditions and processing.The temperature sensors were installed in a 3D array close to the fungus bags about 5 cm in rooms.The light tape was installed on the six walls and also had three colors(Red,Blue,and Green)which could be controlled independently in each room.The control strategy through the analysis of the data collected by all sensors,the current cultivate situation of the cultivation environment was obtained,and the heater,fan,light,and nozzle were regulated according to the strategy to maintain a suitable precision cultivation environment for fungus.To verify the feasibility of the precision cultivation processing and control system,the test result shows that the error of temperature control was about 0℃-1℃,the error of humidity control was about 1%-4%,and the error of illuminance control was about 0-50 lx;All the verification results show that the control system for precision cultivation has high precision and can meet the needs of exploring the"Black 29"fungus cultivation experiment environment.Based on the orthogonal experiment,the best combination of the temperature and humidity for each growth stage was also investigated in this study,further proving the reliability and feasibility of the control system for the precision cultivation of Auricularia auricula.

Studies on the Isolation, Identification and In Vitro Growth Rates of the Three Pathogenic Fungi from Panax notoginseng Cultivated in Wenshan Eparchy

Objective] The aim of this study was to simultaneously isolate and identify the main pathogenic fungi of the root rot, black spot and round spot from the Panax notoginseng plants cultivated in Wenshan Eparchy of Yunnan Province of China. [Method] The pathogenic fungi were isolated and purified by using potato dextrose agar （PDA） medium. The morphological identification was accomplished first according to the colony forms of the fungi when cultivated in vitro, then accord-ing to the symptom characteristics and colony forms of the re-isolated fungi in the reverse inoculation experiments. The molecular identification was performed accord-ing to the amplification and alignment of the internal transcribed space （ITS） se-quences of the fungi. The increases of the diameters and thickness of the colonies of the fungi cultivated in vitro were employed to indicate the growth rates of the fungi. [Results] The consistency of the colony forms and symptom characteristics and the 96%-99% similarities revealed in the ITS sequence alignments al proved that the main pathogenic fungi of the root rot, black spot and round spot of the P. notoginseng plants raised in Wenshan were Cylindrocarpon didymium, Alternaria panax and Mycocentrospora acerina, respectively. When cultivated in vitro in the same temperature, humidity and il umination, the increases of the colony diameters and thickness of C. didymium were the highest, fol owed by those of A. panax, then those of M. acerina. During different cultivation periods, the differences of the colony diameters and thickness of the three fungi al reached extremely significant level. However, at the same cultivation time, the differences of the diameters and thickness among the three fungi only reached significant level. [Conclusion] The main pathogenic fungi which result in the root rot, black spot and round spot of the P. notoginseng in Wenshan are C. didymium, A. panax and M. acerina, respec-tively. When these three diseases break out at the same time, the root rot wil spread fastest, fol owed orderly by the black spot and the round spot.

Ambient Stresses Regulate the Development of the Maize Late Wilt Causing Agent, <i>Harpophora maydis</i>

Late wilt, a severe vascular disease of maize caused by the fungus Harpophora maydis, is characterized by relatively rapid wilting of maize plants before tasseling and until shortly before maturity. In Egypt and Israel, the disease is considered to be a major problem. The pathogen is currently controlled using cultivars of maize having reduced sensitivity, but the fungi can undergo pathogenic variations and become a threat to resistance cultivars as well. The abiotic and biotic factors influencing the infection and disease development are not fully determined. To impose stress in a uniform and chronic manner, we expose the Israeli H. maydis isolates colonies or spores to light, different pH, ionic and hyperosmotic pressures (induced with KCl or sorbitol) or oxygen-related stresses (induced with oxygen enrichment, menadione or peroxide). The optimum pH for both hyphal development and spore germination was pH = 5 - 6, similar to reports for the Egyptian, Indian and Hungarian isolates of H. maydis. In the hyperosmotic regime, hyphal growth was affected in a dosage-dependent curve. Although inoculation under high salt stress also inhibited spore germination, the spores were relatively resistant to this stress in comparison to the hypha. An opposite picture was revealed under menadione/peroxide stress: under high dosage of these compounds, the spore germination was virtually abolished while the colony growth was moderately affected. A daily oxygen enrichment of liquid medium cultures caused an increased growth in the pathogen wet and dry biomass, but daily double treatments led to growth suppression. These findings are a preliminary step towards the inspection of the fungal-host interaction under these different stressful environments. This is important for the future development of new strategies to restrict the disease burst and to protect field corps.