Despite enthusiastic efforts directed at elucidating critical underlying mechanisms towards the identification of novel therapeutic targets for severe acute pancreatitis(SAP), the disease remains without a specific th...Despite enthusiastic efforts directed at elucidating critical underlying mechanisms towards the identification of novel therapeutic targets for severe acute pancreatitis(SAP), the disease remains without a specific therapy to be executed within the first hours to days after onset of symptoms. Although earlier management for SAP should aim to either treat organ failure or reduce infectious complications, the current standard of care for the general management of AP in the first hours to days after onset of symptoms include intravenous fluid replacement, nutritional changes, and the use of analgesics with a close monitoring of vital signs. Furthermore, repeated evaluation of severity is very important, as the condition is particularly unstable in the early stages. In cases where biliary pancreatitis is accompanied by acute cholangitis or in cases where biliary stasis is suspected,an early endoscopic retrograde cholangiopancreatography is recommended.However,practice guidelines regarding the treatment of pancreatitis are suboptimal.In chronic pancreatitis,conservative management strategies include lifestyle modifications and dietary changes followed by analgesics and pancreatic enzyme supplementation.Recently,attention has been focused on phytoceuticals or antioxidants as agents that could surpass the limitations associated with currently available therapies.Because oxidative stress has been shown to play an important role in the pathogenesis of pancreatitis,antioxidants alone or combined with conventional therapy may improve oxidativestress-induced organ damage.Interest in phytoceuticals stems from their potential use as simple,accurate tools for pancreatitis prognostication that could replace older and more tedious methods.Therefore,the use of antioxidative nutrition or phytoceuticals may represent a new direction for clinical research in pancreatitis.In this review article,recent advances in the understanding of the pathogenesis of pancreatitis are discussed and the paradigm shift underway to develop phytoceuticals and antioxidants to treat it is introduced.Despite the promise of studies evaluating the effects of antioxidants/phytoceuticals in pancreatitis,translation to the clinic has thus far been disappointing.However,it is expected that continued research will provide solid evidence to justify the use of antioxidative phytoceuticals in the treatment of pancreatitis.展开更多
目的:筛选重症急性胰腺炎(severe acute pancreatitis,SAP)肝损伤大鼠高迁移率族蛋白B1(high mobility group protein B1,HMGB1)的启动子结合蛋白.方法:牛黄胆酸钠胆胰管逆行注射法制备SAP大鼠模型,与对照组同时处死,取肝组织提取细胞...目的:筛选重症急性胰腺炎(severe acute pancreatitis,SAP)肝损伤大鼠高迁移率族蛋白B1(high mobility group protein B1,HMGB1)的启动子结合蛋白.方法:牛黄胆酸钠胆胰管逆行注射法制备SAP大鼠模型,与对照组同时处死,取肝组织提取细胞核蛋白,PCR扩增末端带生物素标记的HMGB1启动子探针,将核蛋白与HMGB1探针孵育,然后用链亲和素磁珠分离HMGB1启动子-蛋白复合物,分别用0.25 mol/L和1 mol/L NaCl洗脱探针上结合的蛋白,用SDS-PAGE电泳分离样本蛋白,SilverQuest银染试剂染色,比较SAP组和对照组的差异条带并做质谱鉴定.结果:对照组和SAP组共有14条差异条带,质谱鉴定这些差异条带得到H2A、H2B、H3、H4、S100A9转录相关蛋白.结论:该研究筛选到一些SAP肝损伤特异性的HMGB1启动子结合蛋白,对于下一步研究HMGB1在SAP肝损伤过程中的转录调控机制具有重要意义.展开更多
基金Supported by The 2013 grant from the Korean Society of Gin-seng funded by Korean Ginseng Cooperation
文摘Despite enthusiastic efforts directed at elucidating critical underlying mechanisms towards the identification of novel therapeutic targets for severe acute pancreatitis(SAP), the disease remains without a specific therapy to be executed within the first hours to days after onset of symptoms. Although earlier management for SAP should aim to either treat organ failure or reduce infectious complications, the current standard of care for the general management of AP in the first hours to days after onset of symptoms include intravenous fluid replacement, nutritional changes, and the use of analgesics with a close monitoring of vital signs. Furthermore, repeated evaluation of severity is very important, as the condition is particularly unstable in the early stages. In cases where biliary pancreatitis is accompanied by acute cholangitis or in cases where biliary stasis is suspected,an early endoscopic retrograde cholangiopancreatography is recommended.However,practice guidelines regarding the treatment of pancreatitis are suboptimal.In chronic pancreatitis,conservative management strategies include lifestyle modifications and dietary changes followed by analgesics and pancreatic enzyme supplementation.Recently,attention has been focused on phytoceuticals or antioxidants as agents that could surpass the limitations associated with currently available therapies.Because oxidative stress has been shown to play an important role in the pathogenesis of pancreatitis,antioxidants alone or combined with conventional therapy may improve oxidativestress-induced organ damage.Interest in phytoceuticals stems from their potential use as simple,accurate tools for pancreatitis prognostication that could replace older and more tedious methods.Therefore,the use of antioxidative nutrition or phytoceuticals may represent a new direction for clinical research in pancreatitis.In this review article,recent advances in the understanding of the pathogenesis of pancreatitis are discussed and the paradigm shift underway to develop phytoceuticals and antioxidants to treat it is introduced.Despite the promise of studies evaluating the effects of antioxidants/phytoceuticals in pancreatitis,translation to the clinic has thus far been disappointing.However,it is expected that continued research will provide solid evidence to justify the use of antioxidative phytoceuticals in the treatment of pancreatitis.
文摘目的:筛选重症急性胰腺炎(severe acute pancreatitis,SAP)肝损伤大鼠高迁移率族蛋白B1(high mobility group protein B1,HMGB1)的启动子结合蛋白.方法:牛黄胆酸钠胆胰管逆行注射法制备SAP大鼠模型,与对照组同时处死,取肝组织提取细胞核蛋白,PCR扩增末端带生物素标记的HMGB1启动子探针,将核蛋白与HMGB1探针孵育,然后用链亲和素磁珠分离HMGB1启动子-蛋白复合物,分别用0.25 mol/L和1 mol/L NaCl洗脱探针上结合的蛋白,用SDS-PAGE电泳分离样本蛋白,SilverQuest银染试剂染色,比较SAP组和对照组的差异条带并做质谱鉴定.结果:对照组和SAP组共有14条差异条带,质谱鉴定这些差异条带得到H2A、H2B、H3、H4、S100A9转录相关蛋白.结论:该研究筛选到一些SAP肝损伤特异性的HMGB1启动子结合蛋白,对于下一步研究HMGB1在SAP肝损伤过程中的转录调控机制具有重要意义.
