Background As the most widely cultivated fiber crop,cotton production depends on hybridization to unlock the yield potential of current varieties.A deep understanding of genetic dissection is crucial for the cultivati...Background As the most widely cultivated fiber crop,cotton production depends on hybridization to unlock the yield potential of current varieties.A deep understanding of genetic dissection is crucial for the cultivation of enhanced hybrid plants with desired traits,such as high yield and fine fiber quality.In this study,the general combining ability(GCA)and specific combining ability(SCA)of yield and fiber quality of nine cotton parents(six lines and three testers)and eighteen F1 crosses produced using a line×tester mating design were analyzed.Results The results revealed significant effects of genotypes,parents,crosses,and interactions between parents and crosses for most of the studied traits.Moreover,the effects of both additive and non-additive gene actions played a notably significant role in the inheritance of most of the yield and fiber quality attributes.The F1 hybrids of(Giza 90×Aust)×Giza 86,Uzbekistan 1×Giza 97,and Giza 96×Giza 97 demonstrated superior performance due to their favorable integration of high yield attributes and premium fiber quality characteristics.Path analysis revealed that lint yield has the highest positive direct effect on seed cotton yield,while lint percentage showed the highest negative direct effect on seed cotton yield.Principal component analysis identified specific parents and hybrids associated with higher cotton yield,fiber quality,and other agronomic traits.Conclusion This study provides insights into identifying potential single-and three-way cross hybrids with superior cotton yield and fiber quality characteristics,laying a foundation for future research on improving fiber quality in cotton.展开更多
A permanent doubled haploid population fromthe crossing of G.hirsutum × G.barbadensewere developed by means of Vsg,virescentlymarked semigamy line in sea island cotton,which was characterized by a cytologicalmech...A permanent doubled haploid population fromthe crossing of G.hirsutum × G.barbadensewere developed by means of Vsg,virescentlymarked semigamy line in sea island cotton,which was characterized by a cytologicalmechanism for developing haploids with certainconvenience,and thus constructed展开更多
Verticillium dahliae Kleb.is a necrotrophic plant pathogen which causes serious soil borne vascular disease in cotton.The molecular basis the defense response of cotton to this pathogen is
Enzyme adaptations to temperature occur constantly as temperature patterns modulate diurnally and seasonally. These adaptations entail qualitative and/or quantitative metabolic changes that often provide a competitive...Enzyme adaptations to temperature occur constantly as temperature patterns modulate diurnally and seasonally. These adaptations entail qualitative and/or quantitative metabolic changes that often provide a competitive advantage, impact adjustment to new environments, and effect the survival of the species. Changes in isozymes or allozymes, changes in enzyme concentration, modification by substrate and effectors, and metabolic regulation of enzyme function without changing enzyme composition are all possible strategies for adaptation to changes in temperature. The degree of adaptation among cotton cultivars to a specific thermal regime may be difficult to determine from phenotypic responses of the plants. The present study evaluated the thermal sensitivity of Gossypium hirsutum L. and Gossypium barbadense L. cultivars following growth under distinct thermal environments. The metabolic fitness of Gossypium hirsutum L. and Gossypium barbadense L. cultivars showed that the Gossypium hirsutum L. cultivars grown in a 28°C/20°C day/night cycle tended to be better equipped to cope with a 16 h - 38°C treatment than the same cultivars grown in a 38°C/32°C day/night cycle. The Gossypium barbadense L. cultivars, on the other hand, grown in a 38°C/32°C day/night cycle tended to be equipped to cope with a 16 h - 38°C treatment than the same cultivars grown in a 28°C/20°C day/night cycle. The Gossypium hirsutum L. line TX 303 is an exception to these general trends as its responses were similar to the Gossypium barbadense L. St. Vincent and Pima S-7 cottons.展开更多
The GbKTN1 gene was isolated from 10 DPA fiber cells of Gossypium barbadense using 5′RACE/3′RACE.Full-length cDNA of this gene is 2006 bp, including a 113 bp of 5′untranslated region, a 1563 bp of an open reading f...The GbKTN1 gene was isolated from 10 DPA fiber cells of Gossypium barbadense using 5′RACE/3′RACE.Full-length cDNA of this gene is 2006 bp, including a 113 bp of 5′untranslated region, a 1563 bp of an open reading frame(ORF), and a 327 bp of 3′untranslated region (excluding the stop codon TAA). The ORF of GbKTN1 encodes a 521-amino acid protein with a predicted size of 55 kD. Near C-terminal of the deduced protein there is a putative ATP binding site between amino acid residues from 233 to 414. Southern blot analysis indicated that the GbKTN1 was a single copy gene in G barbadense. Combining semi-quantitative RT-PCR with Southern blot hybridization revealed that GbKTN1 expressed in all the organs detected such as roots, stems, leaves and fibers. However, the mRNA of GbKTN1 was the most abundant in fiber cells, while it was the lowest in leaves. The GbKTN1 cDNA was transformed into S. pombe to verify its function on cell elongation. Results showed that most yeast cells over expressing GbKTN1 gene were elongated dramatically with an average length increase of 2.18 times than that of the non-induced cells. Even the morphology of some yeast cells appeared irregularly. To the best of our knowledge this is the first evidence that KTN1 is correlated with cell elongation in vivo.展开更多
文摘Background As the most widely cultivated fiber crop,cotton production depends on hybridization to unlock the yield potential of current varieties.A deep understanding of genetic dissection is crucial for the cultivation of enhanced hybrid plants with desired traits,such as high yield and fine fiber quality.In this study,the general combining ability(GCA)and specific combining ability(SCA)of yield and fiber quality of nine cotton parents(six lines and three testers)and eighteen F1 crosses produced using a line×tester mating design were analyzed.Results The results revealed significant effects of genotypes,parents,crosses,and interactions between parents and crosses for most of the studied traits.Moreover,the effects of both additive and non-additive gene actions played a notably significant role in the inheritance of most of the yield and fiber quality attributes.The F1 hybrids of(Giza 90×Aust)×Giza 86,Uzbekistan 1×Giza 97,and Giza 96×Giza 97 demonstrated superior performance due to their favorable integration of high yield attributes and premium fiber quality characteristics.Path analysis revealed that lint yield has the highest positive direct effect on seed cotton yield,while lint percentage showed the highest negative direct effect on seed cotton yield.Principal component analysis identified specific parents and hybrids associated with higher cotton yield,fiber quality,and other agronomic traits.Conclusion This study provides insights into identifying potential single-and three-way cross hybrids with superior cotton yield and fiber quality characteristics,laying a foundation for future research on improving fiber quality in cotton.
文摘A permanent doubled haploid population fromthe crossing of G.hirsutum × G.barbadensewere developed by means of Vsg,virescentlymarked semigamy line in sea island cotton,which was characterized by a cytologicalmechanism for developing haploids with certainconvenience,and thus constructed
文摘Verticillium dahliae Kleb.is a necrotrophic plant pathogen which causes serious soil borne vascular disease in cotton.The molecular basis the defense response of cotton to this pathogen is
文摘Enzyme adaptations to temperature occur constantly as temperature patterns modulate diurnally and seasonally. These adaptations entail qualitative and/or quantitative metabolic changes that often provide a competitive advantage, impact adjustment to new environments, and effect the survival of the species. Changes in isozymes or allozymes, changes in enzyme concentration, modification by substrate and effectors, and metabolic regulation of enzyme function without changing enzyme composition are all possible strategies for adaptation to changes in temperature. The degree of adaptation among cotton cultivars to a specific thermal regime may be difficult to determine from phenotypic responses of the plants. The present study evaluated the thermal sensitivity of Gossypium hirsutum L. and Gossypium barbadense L. cultivars following growth under distinct thermal environments. The metabolic fitness of Gossypium hirsutum L. and Gossypium barbadense L. cultivars showed that the Gossypium hirsutum L. cultivars grown in a 28°C/20°C day/night cycle tended to be better equipped to cope with a 16 h - 38°C treatment than the same cultivars grown in a 38°C/32°C day/night cycle. The Gossypium barbadense L. cultivars, on the other hand, grown in a 38°C/32°C day/night cycle tended to be equipped to cope with a 16 h - 38°C treatment than the same cultivars grown in a 28°C/20°C day/night cycle. The Gossypium hirsutum L. line TX 303 is an exception to these general trends as its responses were similar to the Gossypium barbadense L. St. Vincent and Pima S-7 cottons.
文摘The GbKTN1 gene was isolated from 10 DPA fiber cells of Gossypium barbadense using 5′RACE/3′RACE.Full-length cDNA of this gene is 2006 bp, including a 113 bp of 5′untranslated region, a 1563 bp of an open reading frame(ORF), and a 327 bp of 3′untranslated region (excluding the stop codon TAA). The ORF of GbKTN1 encodes a 521-amino acid protein with a predicted size of 55 kD. Near C-terminal of the deduced protein there is a putative ATP binding site between amino acid residues from 233 to 414. Southern blot analysis indicated that the GbKTN1 was a single copy gene in G barbadense. Combining semi-quantitative RT-PCR with Southern blot hybridization revealed that GbKTN1 expressed in all the organs detected such as roots, stems, leaves and fibers. However, the mRNA of GbKTN1 was the most abundant in fiber cells, while it was the lowest in leaves. The GbKTN1 cDNA was transformed into S. pombe to verify its function on cell elongation. Results showed that most yeast cells over expressing GbKTN1 gene were elongated dramatically with an average length increase of 2.18 times than that of the non-induced cells. Even the morphology of some yeast cells appeared irregularly. To the best of our knowledge this is the first evidence that KTN1 is correlated with cell elongation in vivo.
