Objective:To screen seaweed Sargassum wightii(S.wightii)for bioactive natural substance against marine ornamental fish bacterial pathogens,and also study the antioxidant properties,brine shrimp toxicity effect.Methods...Objective:To screen seaweed Sargassum wightii(S.wightii)for bioactive natural substance against marine ornamental fish bacterial pathogens,and also study the antioxidant properties,brine shrimp toxicity effect.Methods:Crude extract was made using three solvents(acetone,ethanol and methanol)and screened for antibacterial activity and purified by column chromatography,purified fractions obtained were tested for the activity.The 1st fraction of acetone extract showed maximum activity,this was again subjected for purification and obtained three sub-fractions also tested for the activity.Total phenols and flavonoid contents,reducing power,free radical scavenging activities(DPPH and H_(2)O_(2))and brine shrimp toxicity were also studied using purified acetone extract followed by standard methods.Results:The purified acetone extract showed maximum activity against eight pathogens among ten.GC-MS results revealed two major compounds such as 24-methylene cholesterol(79.9%)and methyl oleate(30.3%)which presented in higher percentage in purified extract and had highest phenols and flavonoid contents,reducing power,free radical scavenging activities,and also showed less toxicity effect.In he present study,the purified extract of S.wightii had potential antibacterial activity against Aeromonas hydrophila[(22.25±0.35)mm]and minimum activity against Streptococcus sp.[(10.00±0.00)mm].The purified extract of S.wightii also had potential total antioxidant activity of(3.87±0.04)μg at 100μg/mL concentration and the lowest activity was exhibited(1.52±0.01)μg at 25μg/mL.Conclusions:The present study concluded that the brown seaweed,S.wightii has potential antimicrobial and antioxidant activities,which can be used in aquaculture industry for treated bacterial diseases in infected fishes.展开更多
Natural antioxidants play a significant role to prevent cell breaking and regenerate cells.Three plants native to India named dwarf morning-glory(Evolvulus alsinoides),Indian banyan(Ficus bengalensis)and chicory(Chico...Natural antioxidants play a significant role to prevent cell breaking and regenerate cells.Three plants native to India named dwarf morning-glory(Evolvulus alsinoides),Indian banyan(Ficus bengalensis)and chicory(Chicorium intybus)are used as medicine in India traditionally to treat diseases.The aim of this study is to evaluate and compare the antioxidant activity as well as to compare phytochemical profiles of the aqueous extracts of the selected three plants.The dried plant powder was extracted with ethanol individually by using the Soxhlet method for eight hours and the solvent was evaporated under reduced pressure.Similarly,the air-dried powder was ex-tracted with water,and the water was evaporated.The thin layer chromatography(TLC)profile of the extracts was determined by using TLC densitometer and the antioxidant activity was deter-mined by the conventional 2,2-diphenyl-1-picrylhydrazyl(DPPH)method.The TLC profile shows that there are no similarities of retention factor(R f)values among the plant extracts which means all three plants contain different chemical compounds.The antioxidant activities of the aqueous extracts at different concentrations show different antioxidant activities.Percentage inhibition is dose dependent,as doses are increasing then percentage inhibition is also increasing for all three plant extracts.Among the selected plants,the highest activity was found in C.intybus and the lowest was in E.alsinoides.The order of the antioxidant activity among the selected plants was C.intybus<F.bengalensis<E.alsinoides.Based on the TLC profile and antioxidant activities,the highest activity of the selected plant species was evaluated.展开更多
基金Supported by University Grants Commission,New Delhi with Grant number(UGC-F.14-2(SC)/2009(SA-III).
文摘Objective:To screen seaweed Sargassum wightii(S.wightii)for bioactive natural substance against marine ornamental fish bacterial pathogens,and also study the antioxidant properties,brine shrimp toxicity effect.Methods:Crude extract was made using three solvents(acetone,ethanol and methanol)and screened for antibacterial activity and purified by column chromatography,purified fractions obtained were tested for the activity.The 1st fraction of acetone extract showed maximum activity,this was again subjected for purification and obtained three sub-fractions also tested for the activity.Total phenols and flavonoid contents,reducing power,free radical scavenging activities(DPPH and H_(2)O_(2))and brine shrimp toxicity were also studied using purified acetone extract followed by standard methods.Results:The purified acetone extract showed maximum activity against eight pathogens among ten.GC-MS results revealed two major compounds such as 24-methylene cholesterol(79.9%)and methyl oleate(30.3%)which presented in higher percentage in purified extract and had highest phenols and flavonoid contents,reducing power,free radical scavenging activities,and also showed less toxicity effect.In he present study,the purified extract of S.wightii had potential antibacterial activity against Aeromonas hydrophila[(22.25±0.35)mm]and minimum activity against Streptococcus sp.[(10.00±0.00)mm].The purified extract of S.wightii also had potential total antioxidant activity of(3.87±0.04)μg at 100μg/mL concentration and the lowest activity was exhibited(1.52±0.01)μg at 25μg/mL.Conclusions:The present study concluded that the brown seaweed,S.wightii has potential antimicrobial and antioxidant activities,which can be used in aquaculture industry for treated bacterial diseases in infected fishes.
文摘Natural antioxidants play a significant role to prevent cell breaking and regenerate cells.Three plants native to India named dwarf morning-glory(Evolvulus alsinoides),Indian banyan(Ficus bengalensis)and chicory(Chicorium intybus)are used as medicine in India traditionally to treat diseases.The aim of this study is to evaluate and compare the antioxidant activity as well as to compare phytochemical profiles of the aqueous extracts of the selected three plants.The dried plant powder was extracted with ethanol individually by using the Soxhlet method for eight hours and the solvent was evaporated under reduced pressure.Similarly,the air-dried powder was ex-tracted with water,and the water was evaporated.The thin layer chromatography(TLC)profile of the extracts was determined by using TLC densitometer and the antioxidant activity was deter-mined by the conventional 2,2-diphenyl-1-picrylhydrazyl(DPPH)method.The TLC profile shows that there are no similarities of retention factor(R f)values among the plant extracts which means all three plants contain different chemical compounds.The antioxidant activities of the aqueous extracts at different concentrations show different antioxidant activities.Percentage inhibition is dose dependent,as doses are increasing then percentage inhibition is also increasing for all three plant extracts.Among the selected plants,the highest activity was found in C.intybus and the lowest was in E.alsinoides.The order of the antioxidant activity among the selected plants was C.intybus<F.bengalensis<E.alsinoides.Based on the TLC profile and antioxidant activities,the highest activity of the selected plant species was evaluated.
