Anther dehiscence controls optimal interaction between pollen and stigma,thereby determining the successful sexual reproduction.The regulators or mechanisms of this process remain elusive.Here,two CRISPR/Cas9 mutants ...Anther dehiscence controls optimal interaction between pollen and stigma,thereby determining the successful sexual reproduction.The regulators or mechanisms of this process remain elusive.Here,two CRISPR/Cas9 mutants of a rice exocyst subunit gene SEC3A,sec3a-1 and sec3a-2,showed anther indehiscence at anthesis and male sterility at maturity.Pollen viability and germination in the mutants were partly defective,whereas their female gametes undergone a normal development.Hybrid or self-pollinated seeds could be produced by artificial pollination,suggesting potential use of a weak sec3a mutant as a female line during hybrid breeding.SEC3A is widely expressed in various tissues,including anther walls.Further results showed an excessive IAA accumulation and no endothecium lignification in sec3a-1/2 anthers.Our findings suggest that SEC3A appears to regulate anther dehiscence by modulating auxin signaling,providing insights into regulation of anther dehiscence and function of exocyst in plants.展开更多
为了探究金葡素SEC2(Staphylococcal enterotoxins C 2)改构蛋白2M-118对小鼠的致呕吐、致热毒性和免疫原性作用,将BALB/c小鼠分为正常组、2M-118腹腔给药组和灌胃给药组,每组10只;通过观察小鼠给药前后状态变化及记录小鼠体质量和体温...为了探究金葡素SEC2(Staphylococcal enterotoxins C 2)改构蛋白2M-118对小鼠的致呕吐、致热毒性和免疫原性作用,将BALB/c小鼠分为正常组、2M-118腹腔给药组和灌胃给药组,每组10只;通过观察小鼠给药前后状态变化及记录小鼠体质量和体温,评价2M-118对小鼠的致呕吐和致热毒性;采用MTT法检测2M-118对小鼠脾淋巴细胞增殖的影响;由ELISA法检测小鼠给药2M-118后血清产生中和抗体的情况。结果表明,2M-118并未致小鼠呕吐或致小鼠体温升高;2M-118可以刺激小鼠脾淋巴细胞增殖,促进脾组织中IFN-γ、TNF-α和IL-2等细胞因子的表达,并刺激机体产生针对2M-118的中和抗体。展开更多
基金supported by the Science Fund for Creative Research Groups of the National Natural Science Foundation of China(32171970)the Chongqing Outstanding Scientists Project(cstc2022ycjh-bgzxm0073)the Natural Science Foundation of Chongqing,China(cstc2021jcyj-cxttX0004)。
文摘Anther dehiscence controls optimal interaction between pollen and stigma,thereby determining the successful sexual reproduction.The regulators or mechanisms of this process remain elusive.Here,two CRISPR/Cas9 mutants of a rice exocyst subunit gene SEC3A,sec3a-1 and sec3a-2,showed anther indehiscence at anthesis and male sterility at maturity.Pollen viability and germination in the mutants were partly defective,whereas their female gametes undergone a normal development.Hybrid or self-pollinated seeds could be produced by artificial pollination,suggesting potential use of a weak sec3a mutant as a female line during hybrid breeding.SEC3A is widely expressed in various tissues,including anther walls.Further results showed an excessive IAA accumulation and no endothecium lignification in sec3a-1/2 anthers.Our findings suggest that SEC3A appears to regulate anther dehiscence by modulating auxin signaling,providing insights into regulation of anther dehiscence and function of exocyst in plants.
文摘为了探究金葡素SEC2(Staphylococcal enterotoxins C 2)改构蛋白2M-118对小鼠的致呕吐、致热毒性和免疫原性作用,将BALB/c小鼠分为正常组、2M-118腹腔给药组和灌胃给药组,每组10只;通过观察小鼠给药前后状态变化及记录小鼠体质量和体温,评价2M-118对小鼠的致呕吐和致热毒性;采用MTT法检测2M-118对小鼠脾淋巴细胞增殖的影响;由ELISA法检测小鼠给药2M-118后血清产生中和抗体的情况。结果表明,2M-118并未致小鼠呕吐或致小鼠体温升高;2M-118可以刺激小鼠脾淋巴细胞增殖,促进脾组织中IFN-γ、TNF-α和IL-2等细胞因子的表达,并刺激机体产生针对2M-118的中和抗体。