From the roots ofPicrorhiza scrophulariiflora, two new secoifidoid glycosides, named picrosecosides Ⅰ and Ⅱ （1, 2） have been isolated. Their structures were elucidated on the basis of chemical and spectropic evide...From the roots ofPicrorhiza scrophulariiflora, two new secoifidoid glycosides, named picrosecosides Ⅰ and Ⅱ （1, 2） have been isolated. Their structures were elucidated on the basis of chemical and spectropic evidences.展开更多
Two new phenylethanoid glycosides,named scroside H（1）,scroside I（2）,and a new secoiridoid glycoside,named picrogentioside I（3）,have been isolated from the underground parts of Picrorhiza scrophulariiflora.Their ...Two new phenylethanoid glycosides,named scroside H（1）,scroside I（2）,and a new secoiridoid glycoside,named picrogentioside I（3）,have been isolated from the underground parts of Picrorhiza scrophulariiflora.Their structures were elucidated on the basis of spectroscopic evidence.展开更多
Eleven new secoiridoid aglycones involving unusual C9-skeleton:swerimilegenins A-F(1-6);bis-C9-skeleton:swerimilegenin G(7);and C_(10)-skeleton:swerimilegenins H−K(8−11),as well as six known ones,were isolated from th...Eleven new secoiridoid aglycones involving unusual C9-skeleton:swerimilegenins A-F(1-6);bis-C9-skeleton:swerimilegenin G(7);and C_(10)-skeleton:swerimilegenins H−K(8−11),as well as six known ones,were isolated from the low-polarity part of the traditional Chinese herb medicine Swertia mileensis.Their structures were determined by extensive spectroscopic data and X-ray diffraction.Biogenetically,swerimilegenin A(1)belonged to 10-nor-secoiridoid,and swerimilegenins B-F(2-6)were 1-nor-secoiridoids.Erythrocentaurin(12)and gentiogenal(15)showed moderate anti-HBV activity on HepG 2.2.15 cell line in vitro.展开更多
Chemical investigation of Syringa velutina Kom. led to the isolation of two new secoiridoid glucosides. Their structures were identified as 6＇-O-（6, 7-dihyrofoliamenthoyl）-8-epi-longisidic acid （syrveoside A, 1） ...Chemical investigation of Syringa velutina Kom. led to the isolation of two new secoiridoid glucosides. Their structures were identified as 6＇-O-（6, 7-dihyrofoliamenthoyl）-8-epi-longisidic acid （syrveoside A, 1） and 6＇-O-menthiafoloyl-8-epi-ldngisidic acid （syrveoside B, 2） on the basis of chemical and physicochemical evidence.展开更多
Swerilactones H–K(1–4)as four unprecedented secoiridoid trimers represent a new type of natural product,which has attracted much interest of natural chemists due to their novel skeletons and promising bioactivity.In...Swerilactones H–K(1–4)as four unprecedented secoiridoid trimers represent a new type of natural product,which has attracted much interest of natural chemists due to their novel skeletons and promising bioactivity.In order to well understand their MS fragmentation behaviors,they were investigated by electrospray ionization ion-trap time-of-flight multistage product ion mass spectrometry(ESI-IT-TOF-MSn)for the first time.The protonated molecules([M?H]?)of swerilactones J and K,and deprotonated molecules([M-H]-)of swerilactones H,J and K were readily observed in the conventional single-stage mass spectra(MS);however only the[M?Cl]-ion for swerilactone I was obtained in negative mode.Based on the MSn study,the fragmentation pathways of swerilactones H and I in negative mode,and swerilactones J and K in both positive and negative modes were proposed.The neutral losses of H_(2)O,CO,CO_(2)and C_(2)H_(4)O moieties are the particular elimination from the precursor ions due to the presence of hydroxyl,d-lactone and 1-O-ethyl moieties in their structures,of which the retro-Diels–Alder cleavage was the most particular dissociation.The fragment ions at m/z 341 and 291 in negative mode can be considered as the diagnostic ions for secoiridoid trimers.This investigation will provide valuable information for their fast characterization from complicated natural mixtures and extensive understanding their structural architectures.展开更多
Objective To study the chemical constituents in the roots of Picrorhiza scrophulariiflora.Methods The chemical constituents in the roots of P.scrophulariiflora were separated and purified with chromatographic methods....Objective To study the chemical constituents in the roots of Picrorhiza scrophulariiflora.Methods The chemical constituents in the roots of P.scrophulariiflora were separated and purified with chromatographic methods.The structures were elucidated by spectroscopic methods and chemical analyses.Results A new secoiridoid glycoside,picrogentioside II(1) was successfully isolated from the roots of P.scrophulariiflora.Conclusion Compound 1 is a new secoiridoid glycoside.展开更多
Three new secoiridoid glycosides,named lonijapoglycol A(1),aldosecolohanin C(2)and aldosecolohanin B(3),together with three known ones(4–6),have been isolated from the flower the buds of Lonicera japonica.All the str...Three new secoiridoid glycosides,named lonijapoglycol A(1),aldosecolohanin C(2)and aldosecolohanin B(3),together with three known ones(4–6),have been isolated from the flower the buds of Lonicera japonica.All the structures were identified by spectroscopic analyses.Lonijapoglycol A(1)expressed significant anti-inflammatory activity to inhibit the release ofβ-glucuronidase induced by platelet-activating factor in rat polymorphonuclear leukocytes with an IC50 value of 3.76μmol·L^-1.展开更多
Objective To study the chemical constituents of Swertia cincta. Methods Preparative liquid chromatography was employed. The structures of the compounds were elucidated by spectroscopic analysis. isolated from S. cinct...Objective To study the chemical constituents of Swertia cincta. Methods Preparative liquid chromatography was employed. The structures of the compounds were elucidated by spectroscopic analysis. isolated from S. cincta and identified as Results Three secoiridoid glycosides were 8-methoxyl-eustomorusside （1）, secoiridoids eustomorusside （2）, and eustomoside （3）. Conclusion Compound 1 is a new secoiridoid glycoside. Compounds 2 and 3 are isolated from this plant for the first time.展开更多
Objective:To simultaneously investigate the pharmacokinetics of gentiopicroside,sweroside,and swertiamarin,which are constituents of Gentianella acuta,by developing and validating a simple,sensitive,and fast ultra-hig...Objective:To simultaneously investigate the pharmacokinetics of gentiopicroside,sweroside,and swertiamarin,which are constituents of Gentianella acuta,by developing and validating a simple,sensitive,and fast ultra-high-performance liquid chromatography–tandem mass spectrometry method.Materials and Methods:Blood samples were collected from the forward limb veins of six beagle dogs following oral gavage with G.acuta,the whole plant extract(39.90 mg/kg).Plasma samples were processed using liquid–liquid extraction.The analytes and paeoniflorin(internal standard[IS])were separated using an Acquity?UPLC ethylene bridged hybrid amide column(2.1 mm×100 mm,1.7μm)with isocratic elution using a mobile phase consisting of acetonitrile and 0.1%formic acid in water(80:20,v/v)at a flow rate of 0.4 mL/min.Quantification was performed using multiple reaction monitoring of the fragmentation transitions at m/z 401.1→179.0,403.1→195.0,419.1→179.0,and 525.2→449.1 for gentiopicroside,sweroside,swertiamarin,and the IS,respectively.Results:The linearity of the analytical response was good and the calibration curves were linear over concentration ranges of 1.20–192.0,0.40–159.0,and 0.20–209.3 ng/mL for gentiopicroside,sweroside,and swertiamarin,respectively.The extraction recovery was in the range of 84.72%–91.34%,84.58%–93.43%,and 82.75%–91.37%for gentiopicroside,sweroside,and swertiamarin,respectively.Conclusions:The method was successfully used to evaluate the pharmacokinetic parameters of gentiopicroside,sweroside,and swertiamarin in beagle dogs.展开更多
基金National Key Project of Scientific and Technical Supporting Programs funded by Ministry of Science & Technology of China(No.2006BAD31B05).
文摘From the roots ofPicrorhiza scrophulariiflora, two new secoifidoid glycosides, named picrosecosides Ⅰ and Ⅱ （1, 2） have been isolated. Their structures were elucidated on the basis of chemical and spectropic evidences.
基金supported by National Natural Science Foundation of China(No.30800835)National Key Project of Scientific and Technical Supporting Programs funded by Ministry of Science & Technology of China(No. 2006BAD31B05)
文摘Two new phenylethanoid glycosides,named scroside H（1）,scroside I（2）,and a new secoiridoid glycoside,named picrogentioside I（3）,have been isolated from the underground parts of Picrorhiza scrophulariiflora.Their structures were elucidated on the basis of spectroscopic evidence.
基金This work was funded by the National Natural Science Foundation of China and Yunnan Province(U0832603)the National Science Foundation of China for Distinguished Young Scholars(81025023)+1 种基金the West Light Foundation of the Chinese Academy of Sciences,the International Foundation for Science(No.F/5202-1)the Youth Innovation Promotion Association,CAS.
文摘Eleven new secoiridoid aglycones involving unusual C9-skeleton:swerimilegenins A-F(1-6);bis-C9-skeleton:swerimilegenin G(7);and C_(10)-skeleton:swerimilegenins H−K(8−11),as well as six known ones,were isolated from the low-polarity part of the traditional Chinese herb medicine Swertia mileensis.Their structures were determined by extensive spectroscopic data and X-ray diffraction.Biogenetically,swerimilegenin A(1)belonged to 10-nor-secoiridoid,and swerimilegenins B-F(2-6)were 1-nor-secoiridoids.Erythrocentaurin(12)and gentiogenal(15)showed moderate anti-HBV activity on HepG 2.2.15 cell line in vitro.
基金supported by the National Key Project of Scientific and Technical Supporting Programs fundedby Ministry of Science & Technology of China(No.2006BAD31B05)
文摘Chemical investigation of Syringa velutina Kom. led to the isolation of two new secoiridoid glucosides. Their structures were identified as 6＇-O-（6, 7-dihyrofoliamenthoyl）-8-epi-longisidic acid （syrveoside A, 1） and 6＇-O-menthiafoloyl-8-epi-ldngisidic acid （syrveoside B, 2） on the basis of chemical and physicochemical evidence.
文摘Swerilactones H–K(1–4)as four unprecedented secoiridoid trimers represent a new type of natural product,which has attracted much interest of natural chemists due to their novel skeletons and promising bioactivity.In order to well understand their MS fragmentation behaviors,they were investigated by electrospray ionization ion-trap time-of-flight multistage product ion mass spectrometry(ESI-IT-TOF-MSn)for the first time.The protonated molecules([M?H]?)of swerilactones J and K,and deprotonated molecules([M-H]-)of swerilactones H,J and K were readily observed in the conventional single-stage mass spectra(MS);however only the[M?Cl]-ion for swerilactone I was obtained in negative mode.Based on the MSn study,the fragmentation pathways of swerilactones H and I in negative mode,and swerilactones J and K in both positive and negative modes were proposed.The neutral losses of H_(2)O,CO,CO_(2)and C_(2)H_(4)O moieties are the particular elimination from the precursor ions due to the presence of hydroxyl,d-lactone and 1-O-ethyl moieties in their structures,of which the retro-Diels–Alder cleavage was the most particular dissociation.The fragment ions at m/z 341 and 291 in negative mode can be considered as the diagnostic ions for secoiridoid trimers.This investigation will provide valuable information for their fast characterization from complicated natural mixtures and extensive understanding their structural architectures.
基金National Key Project of Scientific and Technical Supporting Programs funded by Ministry of Science&Technology of China(2006BAD31B05)
文摘Objective To study the chemical constituents in the roots of Picrorhiza scrophulariiflora.Methods The chemical constituents in the roots of P.scrophulariiflora were separated and purified with chromatographic methods.The structures were elucidated by spectroscopic methods and chemical analyses.Results A new secoiridoid glycoside,picrogentioside II(1) was successfully isolated from the roots of P.scrophulariiflora.Conclusion Compound 1 is a new secoiridoid glycoside.
基金supported by the Standardization Project Fund of Administration of Traditional Chinese Medicine(No.ZYBZH-Y-SD-32)the Specialized Fund for Dependent Innovation of Shandong Province(No.2013CXC20401)
文摘Three new secoiridoid glycosides,named lonijapoglycol A(1),aldosecolohanin C(2)and aldosecolohanin B(3),together with three known ones(4–6),have been isolated from the flower the buds of Lonicera japonica.All the structures were identified by spectroscopic analyses.Lonijapoglycol A(1)expressed significant anti-inflammatory activity to inhibit the release ofβ-glucuronidase induced by platelet-activating factor in rat polymorphonuclear leukocytes with an IC50 value of 3.76μmol·L^-1.
基金NSFC(NO.21262048)Youth Foundation of Lincang Teachers’College(LCSZL201204)
文摘Objective To study the chemical constituents of Swertia cincta. Methods Preparative liquid chromatography was employed. The structures of the compounds were elucidated by spectroscopic analysis. isolated from S. cincta and identified as Results Three secoiridoid glycosides were 8-methoxyl-eustomorusside （1）, secoiridoids eustomorusside （2）, and eustomoside （3）. Conclusion Compound 1 is a new secoiridoid glycoside. Compounds 2 and 3 are isolated from this plant for the first time.
基金supported by the Research Project of Heilongjiang University of Chinese Medicine“Supporting Plan for Excellent Innovative Talents”(2018RCD03)Heilongjiang Provincial Science Fund Project(H2018056)+2 种基金Heilongjiang Post-doctoral Research Start Fund Project(LBH-Q16214)General Projects of NSFC(81973439,81872979,and 81803686)Research Fund of Heilongjiang University of Chinese Medicine(201504)
文摘Objective:To simultaneously investigate the pharmacokinetics of gentiopicroside,sweroside,and swertiamarin,which are constituents of Gentianella acuta,by developing and validating a simple,sensitive,and fast ultra-high-performance liquid chromatography–tandem mass spectrometry method.Materials and Methods:Blood samples were collected from the forward limb veins of six beagle dogs following oral gavage with G.acuta,the whole plant extract(39.90 mg/kg).Plasma samples were processed using liquid–liquid extraction.The analytes and paeoniflorin(internal standard[IS])were separated using an Acquity?UPLC ethylene bridged hybrid amide column(2.1 mm×100 mm,1.7μm)with isocratic elution using a mobile phase consisting of acetonitrile and 0.1%formic acid in water(80:20,v/v)at a flow rate of 0.4 mL/min.Quantification was performed using multiple reaction monitoring of the fragmentation transitions at m/z 401.1→179.0,403.1→195.0,419.1→179.0,and 525.2→449.1 for gentiopicroside,sweroside,swertiamarin,and the IS,respectively.Results:The linearity of the analytical response was good and the calibration curves were linear over concentration ranges of 1.20–192.0,0.40–159.0,and 0.20–209.3 ng/mL for gentiopicroside,sweroside,and swertiamarin,respectively.The extraction recovery was in the range of 84.72%–91.34%,84.58%–93.43%,and 82.75%–91.37%for gentiopicroside,sweroside,and swertiamarin,respectively.Conclusions:The method was successfully used to evaluate the pharmacokinetic parameters of gentiopicroside,sweroside,and swertiamarin in beagle dogs.
