Recently, a kind of hybrid solution MEA-methanol shows a better CO_2 capture performance over aqueous MEA solution. However, the vaporization of methanol is the biggest disadvantage that hinders its application, so it...Recently, a kind of hybrid solution MEA-methanol shows a better CO_2 capture performance over aqueous MEA solution. However, the vaporization of methanol is the biggest disadvantage that hinders its application, so it is necessary to minimize the vaporization of methanol during both the absorption and regeneration processes. In this work, two kinds of hybrid solutions were studied and compared with aqueous MEA solution and MEA-methanol solution, including MEA/TEA/methanol solution and MEA/glycerol/methanol solution. The absorption property of MEA/glycerol/methanol solution is better than aqueous MEA solution within a certain period of time and the absorption property of MEA/TEA/methanol solution is too poor to be used in CO_2 capture. By increasing the concentration of TEA and decreasing the concentration of MEA, the absorption rate, CO_2 capture efficiency and absorption capacity all decreased. Upon adding glycerol, the cyclic capacity decreased and the generation temperature increased, and moreover, the density and viscosity also increased considerably. So after adding TEA and glycerol, the CO_2 capture performance of MEAmethanol solvent cannot be improved.展开更多
The method of laser capture microdissection (LCM) combined with suppressive subtractive hybridization (SSH) was developed to isolate specific germ cells from human testis sections and to identify the genes expressed d...The method of laser capture microdissection (LCM) combined with suppressive subtractive hybridization (SSH) was developed to isolate specific germ cells from human testis sections and to identify the genes expressed during differentiation and development. In the present study, over 10,000 primary spermatocytes and round spermatid cells were successfully isolated by LCM. Using the cDNAs from primary spermatocytes and round spermatids, SSH cDNAs library of primary spermatocyte-specific was constructed. The average insert size of the cDNA isolated from 75 randomly picked white clones was 500 bp, ranging from 250 bp to 1.7 kb. Using the dot-blot method, a total of 421 clones were examined, resulting in the identification of 390 positive clones emitting strong signals. Partial sequence of cDNAs prepared from each clone was determined with an overall success rate of 84.4%. Genes encoding cytochrome c oxidase II and the rescue factor-humanin were most frequently expressed in primary spermatocytes, suggesting their roles involved in meiosis.展开更多
Motivated by inconveniences of present hybrid methods,a gradient-augmented hybrid interface capturing method（GAHM） is presented for incompressible two-phase flow.A front tracking method（FTM） is used as the skeleto...Motivated by inconveniences of present hybrid methods,a gradient-augmented hybrid interface capturing method（GAHM） is presented for incompressible two-phase flow.A front tracking method（FTM） is used as the skeleton of the GAHM for low mass loss and resources.Smooth eulerian level set values are calculated from the FTM interface,and are used for a local interface reconstruction.The reconstruction avoids marker particle redistribution and enables an automatic treatment of interfacial topology change.The cubic Hermit interpolation is employed in all steps of the GAHM to capture subgrid structures within a single spacial cell.The performance of the GAHM is carefully evaluated in a benchmark test.Results show significant improvements of mass loss,clear subgrid structures,highly accurate derivatives（normals and curvatures） and low cost.The GAHM is further coupled with an incompressible multiphase flow solver,Super CE/SE,for more complex and practical applications.The updated solver is evaluated through comparison with an early droplet research.展开更多
Hybrid adsorbents for COcapture were prepared by coassembling laponite（LP） nanosheets and 1-nbutyl-3-methylimidazolium chloride（BMIMCl）. The prepared BMIMCl/LP layered hybrids were systematically characterized. Th...Hybrid adsorbents for COcapture were prepared by coassembling laponite（LP） nanosheets and 1-nbutyl-3-methylimidazolium chloride（BMIMCl）. The prepared BMIMCl/LP layered hybrids were systematically characterized. The interlayer distance of the BMIMCl/LP layered hybrids expanded with an increasing concentration of BMIMCl, indicating that cumulative BMIMCl was intercalated into the LP layers. The efficiency of BMIMCl toward COcapture was significantly enhanced after it was immobilized within LP layers.展开更多
In this paper,a hybrid passive/active vibration(HPAV)controller of a loosely connected spacecraft consisting of a servicing satellite,a target and an X-shape structure isolator is first proposed to suppress vibrations...In this paper,a hybrid passive/active vibration(HPAV)controller of a loosely connected spacecraft consisting of a servicing satellite,a target and an X-shape structure isolator is first proposed to suppress vibrations of the system when subjected to the impulsive external excitations during the on-orbit missions.The passive dynamic response of the combined system can be adjusted appropriately to achieve the desired vibration isolation performance by tuning the structural parameters of the bio-inspired X-shape structure.Moreover,the adaptive control design through dynamic scaling technique is selected as the active component to maintain high vibration isolation performance in the presence of parameter uncertainties such as mass of the satellite platform,the damping and rotation friction coefficients of the X-shape structure.Compared with the pure passive system and the traditional spring-mass-damper(SMD)isolator,the HPAV strategy witnesses lower transmissibility,smaller vibration amplitude and higher convergence rate when subjected to the post-capture impact.Numerical simulations demonstrate the feasibility and validity of the proposed hybrid control scheme in suppressing vibrations of the free-floating spacecraft.展开更多
New indica and japonica hybrid rice cultivars,such as the Yongyou series,provide farmers with very high yield potential.However,information on their canopy light capture and solar radiation use efficiency in the late ...New indica and japonica hybrid rice cultivars,such as the Yongyou series,provide farmers with very high yield potential.However,information on their canopy light capture and solar radiation use efficiency in the late season is limited.Field experiments were performed to compare the radiation-use parameters of four rice types:indica rice(IR),inbred japonica rice(IJR),hybrid japonica rice(HJR),and hybrid indica/japonica rice(HIJR),from 2016 to 2018 during the late season in Hangzhou,China.The grain yield,aboveground biomass,intercepted solar radiation(SI),and radiation-use efficiency(RUE)of the HIJR were on average respectively 13.4%–53.4%,14.3%–30.6%,7.6%–21.4%,and 8.2%–14.9%higher than those of the HJR,IJR,and IR.The leaf area index(LAI)of the HIJR was 18.2%–57.0%greater than that of the IJR and HJR at four growth stages,resulting in respectively 17.8%–38.5%and 10.7%–42.8%greater canopy light interception rates(LIR)and amount of intercepted solar radiation during the vegetative stage.The prolonged grain-filling stage also led to respectively 33.9%–52.6%and 30.5%–51.4%increases in amounts of incident and intercepted radiation for the HIJR relative to the IR during grain filling.These results indicate that the SI superiority of the HIJR was caused by canopy closure as rapid as that of the IR during the vegetative stage(greater LAI and canopy LIR during the growing season)and a grain-filling stage as long as that of the HJR.For grain-filling stage,differences in leaf Pn between HIJR,IR,and IJR were not significant,suggesting that the greater RUE of the HIJR(12.7%–52.8%higher)than that of the other rice types resulted from improved canopy architecture after flowering(FL).Principal components analysis(PCA)revealed that the superiority of the HIJR in terms of solar radiation use resulted from the greater canopy light capture capability of IR and the prolonged growth period(especially during grain filling)of japonica rice in the late growing season.展开更多
The detection and analysis of circulating tumor cells （CTCs） from patients＇ blood is important to assess tumor status; however, it remains a challenge. In the present study, we developed a programmable DNA-responsi...The detection and analysis of circulating tumor cells （CTCs） from patients＇ blood is important to assess tumor status; however, it remains a challenge. In the present study, we developed a programmable DNA-responsive microchip for the highly efficient capture and nondestructive release of CTCs via nucleic acid hybridization. Transparent and patternable substrates with hierarchical architectures were integrated into the microchip with herringbone grooves, resulting in greatly enhanced cell-surface interaction via herringbone micromixers, more binding sites, and better matched topographical interactions. In combination with a high-affinity aptamer, target cancer cells were specifically and efficiently captured on the chip. Captured cancer cells were gently released from the chip under physiological conditions using toehold-mediated strand displacement, without any destructive factors for cells or substrates. More importantly, aptamercontaining DNA sequences on the surface of the retrieved cancer cells could be further amplified by polymerase chain reaction （PCR）, facilitating the detection of cell surface biomarkers and characterization of the CTCs. Furthermore, this system was extensively applied to the capture and release of CTCs from patients＇ blood samples, demonstrating a promising high-performance platform for CTC enrichment, release, and characterization.展开更多
基金supported by the Sinopec Ningbo Engineering Co., Ltd. (No. 14850000-14-ZC0609-0003, H8XY-0032)
文摘Recently, a kind of hybrid solution MEA-methanol shows a better CO_2 capture performance over aqueous MEA solution. However, the vaporization of methanol is the biggest disadvantage that hinders its application, so it is necessary to minimize the vaporization of methanol during both the absorption and regeneration processes. In this work, two kinds of hybrid solutions were studied and compared with aqueous MEA solution and MEA-methanol solution, including MEA/TEA/methanol solution and MEA/glycerol/methanol solution. The absorption property of MEA/glycerol/methanol solution is better than aqueous MEA solution within a certain period of time and the absorption property of MEA/TEA/methanol solution is too poor to be used in CO_2 capture. By increasing the concentration of TEA and decreasing the concentration of MEA, the absorption rate, CO_2 capture efficiency and absorption capacity all decreased. Upon adding glycerol, the cyclic capacity decreased and the generation temperature increased, and moreover, the density and viscosity also increased considerably. So after adding TEA and glycerol, the CO_2 capture performance of MEAmethanol solvent cannot be improved.
文摘The method of laser capture microdissection (LCM) combined with suppressive subtractive hybridization (SSH) was developed to isolate specific germ cells from human testis sections and to identify the genes expressed during differentiation and development. In the present study, over 10,000 primary spermatocytes and round spermatid cells were successfully isolated by LCM. Using the cDNAs from primary spermatocytes and round spermatids, SSH cDNAs library of primary spermatocyte-specific was constructed. The average insert size of the cDNA isolated from 75 randomly picked white clones was 500 bp, ranging from 250 bp to 1.7 kb. Using the dot-blot method, a total of 421 clones were examined, resulting in the identification of 390 positive clones emitting strong signals. Partial sequence of cDNAs prepared from each clone was determined with an overall success rate of 84.4%. Genes encoding cytochrome c oxidase II and the rescue factor-humanin were most frequently expressed in primary spermatocytes, suggesting their roles involved in meiosis.
基金Project supported by the National Natural Science Foundation of China(Grant Nos.10972010,11028206,11371069,11372052,11402029,and 11472060)the Science and Technology Development Foundation of China Academy of Engineering Physics(CAEP),China(Grant No.2014B0201030)the Defense Industrial Technology Development Program of China(Grant No.B1520132012)
文摘Motivated by inconveniences of present hybrid methods,a gradient-augmented hybrid interface capturing method（GAHM） is presented for incompressible two-phase flow.A front tracking method（FTM） is used as the skeleton of the GAHM for low mass loss and resources.Smooth eulerian level set values are calculated from the FTM interface,and are used for a local interface reconstruction.The reconstruction avoids marker particle redistribution and enables an automatic treatment of interfacial topology change.The cubic Hermit interpolation is employed in all steps of the GAHM to capture subgrid structures within a single spacial cell.The performance of the GAHM is carefully evaluated in a benchmark test.Results show significant improvements of mass loss,clear subgrid structures,highly accurate derivatives（normals and curvatures） and low cost.The GAHM is further coupled with an incompressible multiphase flow solver,Super CE/SE,for more complex and practical applications.The updated solver is evaluated through comparison with an early droplet research.
基金sponsored by the National Science Foundation(CMMI-1562907)the financial support from the National Natural Science Foundation of China(51678511 and 51308484)+4 种基金the Open Fund of Key Laboratory of Mineralogy and Metallogeny in Chinese Academy of Sciences(KLMM20150104)the Natural Science Foundation of Hunan Province(13JJ4049)the Education Department Fund of Hunan Province(14C1094)the Major Talent Training Program of Xiangtan University(16PYZ09)the Specialized Research Fund for the Doctoral Program of Xiangtan University(12QDZ18)
文摘Hybrid adsorbents for COcapture were prepared by coassembling laponite（LP） nanosheets and 1-nbutyl-3-methylimidazolium chloride（BMIMCl）. The prepared BMIMCl/LP layered hybrids were systematically characterized. The interlayer distance of the BMIMCl/LP layered hybrids expanded with an increasing concentration of BMIMCl, indicating that cumulative BMIMCl was intercalated into the LP layers. The efficiency of BMIMCl toward COcapture was significantly enhanced after it was immobilized within LP layers.
文摘In this paper,a hybrid passive/active vibration(HPAV)controller of a loosely connected spacecraft consisting of a servicing satellite,a target and an X-shape structure isolator is first proposed to suppress vibrations of the system when subjected to the impulsive external excitations during the on-orbit missions.The passive dynamic response of the combined system can be adjusted appropriately to achieve the desired vibration isolation performance by tuning the structural parameters of the bio-inspired X-shape structure.Moreover,the adaptive control design through dynamic scaling technique is selected as the active component to maintain high vibration isolation performance in the presence of parameter uncertainties such as mass of the satellite platform,the damping and rotation friction coefficients of the X-shape structure.Compared with the pure passive system and the traditional spring-mass-damper(SMD)isolator,the HPAV strategy witnesses lower transmissibility,smaller vibration amplitude and higher convergence rate when subjected to the post-capture impact.Numerical simulations demonstrate the feasibility and validity of the proposed hybrid control scheme in suppressing vibrations of the free-floating spacecraft.
基金This research was supported in part by grants from the National Key Research and Development Program of China(2016YFD0300108,2016YFD0300208-02)the National Natural Science Foundation of China(31671638)+1 种基金the China Agriculture Research System(CARS-01-04A)Central Public Interest Scientific Institution Basal Research Fund(2017RG004-1).
文摘New indica and japonica hybrid rice cultivars,such as the Yongyou series,provide farmers with very high yield potential.However,information on their canopy light capture and solar radiation use efficiency in the late season is limited.Field experiments were performed to compare the radiation-use parameters of four rice types:indica rice(IR),inbred japonica rice(IJR),hybrid japonica rice(HJR),and hybrid indica/japonica rice(HIJR),from 2016 to 2018 during the late season in Hangzhou,China.The grain yield,aboveground biomass,intercepted solar radiation(SI),and radiation-use efficiency(RUE)of the HIJR were on average respectively 13.4%–53.4%,14.3%–30.6%,7.6%–21.4%,and 8.2%–14.9%higher than those of the HJR,IJR,and IR.The leaf area index(LAI)of the HIJR was 18.2%–57.0%greater than that of the IJR and HJR at four growth stages,resulting in respectively 17.8%–38.5%and 10.7%–42.8%greater canopy light interception rates(LIR)and amount of intercepted solar radiation during the vegetative stage.The prolonged grain-filling stage also led to respectively 33.9%–52.6%and 30.5%–51.4%increases in amounts of incident and intercepted radiation for the HIJR relative to the IR during grain filling.These results indicate that the SI superiority of the HIJR was caused by canopy closure as rapid as that of the IR during the vegetative stage(greater LAI and canopy LIR during the growing season)and a grain-filling stage as long as that of the HJR.For grain-filling stage,differences in leaf Pn between HIJR,IR,and IJR were not significant,suggesting that the greater RUE of the HIJR(12.7%–52.8%higher)than that of the other rice types resulted from improved canopy architecture after flowering(FL).Principal components analysis(PCA)revealed that the superiority of the HIJR in terms of solar radiation use resulted from the greater canopy light capture capability of IR and the prolonged growth period(especially during grain filling)of japonica rice in the late growing season.
基金This work was supported by the National Natural Science Foundation of China （NSFC） （Nos. 21432008, 91413109 and 21575110）. China Postdoctoral Innovative Talent Support Program of China （No. BX201700176）.
文摘The detection and analysis of circulating tumor cells （CTCs） from patients＇ blood is important to assess tumor status; however, it remains a challenge. In the present study, we developed a programmable DNA-responsive microchip for the highly efficient capture and nondestructive release of CTCs via nucleic acid hybridization. Transparent and patternable substrates with hierarchical architectures were integrated into the microchip with herringbone grooves, resulting in greatly enhanced cell-surface interaction via herringbone micromixers, more binding sites, and better matched topographical interactions. In combination with a high-affinity aptamer, target cancer cells were specifically and efficiently captured on the chip. Captured cancer cells were gently released from the chip under physiological conditions using toehold-mediated strand displacement, without any destructive factors for cells or substrates. More importantly, aptamercontaining DNA sequences on the surface of the retrieved cancer cells could be further amplified by polymerase chain reaction （PCR）, facilitating the detection of cell surface biomarkers and characterization of the CTCs. Furthermore, this system was extensively applied to the capture and release of CTCs from patients＇ blood samples, demonstrating a promising high-performance platform for CTC enrichment, release, and characterization.
