A capillary electrophoresis (CE) method has been firstly used for the separation of the therapeutically important xanthones from Securidaca inappendiculata. The separation of the nine xanthones was systematically opt...A capillary electrophoresis (CE) method has been firstly used for the separation of the therapeutically important xanthones from Securidaca inappendiculata. The separation of the nine xanthones was systematically optimized with respect to pH, concentration of running buffers, addition of sulfated b-CD, applied voltage and column temperature. Baseline separation was achieved for the nine xanthones in less than 15 minutes using a background electrolyte consisting of 200 mmol/L borate (pH 9.5) and 10 mmol/L sulfated b-CD.展开更多
A new benzophenone, securiphenone A was isolated from the roots of Securidaca inappendiculata. Its structure was. determined as 2, 3-methylenedioxy-4-methoxybenzophenone by spectroscopie methods.
Objective:Securidaca inappendiculata is a medicinal plant frequently used in the treatment of inflammatory diseases in south China.In this study,we aimed to explore its bioactive constituent which contributes to the a...Objective:Securidaca inappendiculata is a medicinal plant frequently used in the treatment of inflammatory diseases in south China.In this study,we aimed to explore its bioactive constituent which contributes to the anti-inflammatory activity.Methods:Polyphenol-enriched and polyphenol-deprived fractions(PRF and PDF,respectively)were separated from the ethanolic extract by HPD300 macroporous resin-based method,and their antiinflammatory activities were investigated on a lipopolysaccharide(LPS)-induced acute lung injury(ALI)model in rats.The possible mechanism of action in alleviating acute inflammation was studied using RAW264.7 cells.Results:Both Folin-Ciocalteu and^(1)H nuclear magnetic resonance(NMR)analyses showed that polyphenolic content in PRF was approximately 10 times higher than that of PDF,and this observation reflected in their antioxidative capacities.PRF but not PDF significantly decreased the level of malondialdehyde,suppressed the expression of nicotinamide phosphoribosyltransferase(NAMPT)protein,and improved the severity of ALI in rats.PRF at 10μg/mL effectively downregulated the expression of proteins NAMPT,HMGB1,TLR4,and p-p65,and scavenged the intracellular reactive oxygen species(ROS)in LPS-primed RAW264.7 cells.N-acetyl-L-cysteine exhibited similar inhibitory effects on ROS production and NAMPT-mediated TLR4/NF-κB activation in vitro,whereas nicotinamide mononucleotide antagonized all the changes induced by PRF during cotreatments.Conclusion:As an antioxidant,PRF exhibited potent anti-inflammatory activity under both in vivo and in vitro conditions by downregulating NAMPT and TLR4/NF-κB.Accordingly,polyphenols were identified as important bioactive constituents in S.inappendiculata targeting oxidative stress-sensitive proinflammatory pathways.展开更多
A microemulsion electrokinetic chromatography method has been firstly used for the separation of the therapeutically important xanthones from Securidaca inappendiculata. The separation of the nine xanthones was syste...A microemulsion electrokinetic chromatography method has been firstly used for the separation of the therapeutically important xanthones from Securidaca inappendiculata. The separation of the nine xanthones was systematically optimized with respect to pH, composition of microemulsion, addition of cyclodextrins, applied voltage and column temperature. Baseline separation was successfully achieved for the nine xanthones, which was also compared with that by micellar electrokinetic chromatography.展开更多
基金The authors would like to acknowledge Agilent Technologies for providing the capillary electrophoresis system and Bioanalytical System for present of sulfated b-CD. The study is supported by NSFC Grant No. 29875001.
文摘A capillary electrophoresis (CE) method has been firstly used for the separation of the therapeutically important xanthones from Securidaca inappendiculata. The separation of the nine xanthones was systematically optimized with respect to pH, concentration of running buffers, addition of sulfated b-CD, applied voltage and column temperature. Baseline separation was achieved for the nine xanthones in less than 15 minutes using a background electrolyte consisting of 200 mmol/L borate (pH 9.5) and 10 mmol/L sulfated b-CD.
文摘A new benzophenone, securiphenone A was isolated from the roots of Securidaca inappendiculata. Its structure was. determined as 2, 3-methylenedioxy-4-methoxybenzophenone by spectroscopie methods.
基金supported by National Natural Science Foundation of China(81603388,81973828 and 81173596)Major Project of Natural Science Foundation of the Department of Education of Anhui province(KJ2019ZD32)+1 种基金Funding of"Peak"Training Program for Scientific Research of Yijishan Hospital,Wannan Medical College(GF2019J01)Key Project of Natural Science Foundation of Anhui Province for College Scholar(KJ2019A0416 and KJ2018A0249)。
文摘Objective:Securidaca inappendiculata is a medicinal plant frequently used in the treatment of inflammatory diseases in south China.In this study,we aimed to explore its bioactive constituent which contributes to the anti-inflammatory activity.Methods:Polyphenol-enriched and polyphenol-deprived fractions(PRF and PDF,respectively)were separated from the ethanolic extract by HPD300 macroporous resin-based method,and their antiinflammatory activities were investigated on a lipopolysaccharide(LPS)-induced acute lung injury(ALI)model in rats.The possible mechanism of action in alleviating acute inflammation was studied using RAW264.7 cells.Results:Both Folin-Ciocalteu and^(1)H nuclear magnetic resonance(NMR)analyses showed that polyphenolic content in PRF was approximately 10 times higher than that of PDF,and this observation reflected in their antioxidative capacities.PRF but not PDF significantly decreased the level of malondialdehyde,suppressed the expression of nicotinamide phosphoribosyltransferase(NAMPT)protein,and improved the severity of ALI in rats.PRF at 10μg/mL effectively downregulated the expression of proteins NAMPT,HMGB1,TLR4,and p-p65,and scavenged the intracellular reactive oxygen species(ROS)in LPS-primed RAW264.7 cells.N-acetyl-L-cysteine exhibited similar inhibitory effects on ROS production and NAMPT-mediated TLR4/NF-κB activation in vitro,whereas nicotinamide mononucleotide antagonized all the changes induced by PRF during cotreatments.Conclusion:As an antioxidant,PRF exhibited potent anti-inflammatory activity under both in vivo and in vitro conditions by downregulating NAMPT and TLR4/NF-κB.Accordingly,polyphenols were identified as important bioactive constituents in S.inappendiculata targeting oxidative stress-sensitive proinflammatory pathways.
文摘A microemulsion electrokinetic chromatography method has been firstly used for the separation of the therapeutically important xanthones from Securidaca inappendiculata. The separation of the nine xanthones was systematically optimized with respect to pH, composition of microemulsion, addition of cyclodextrins, applied voltage and column temperature. Baseline separation was successfully achieved for the nine xanthones, which was also compared with that by micellar electrokinetic chromatography.
