This paper aimed to optimize extracting conditions of SOD in Sedum aizoon by response surface method. First,the single factor optimization tests were carried out. Three single factors were time,p H and liquid-solid ra...This paper aimed to optimize extracting conditions of SOD in Sedum aizoon by response surface method. First,the single factor optimization tests were carried out. Three single factors were time,p H and liquid-solid ratio respectively. By Design expert 8. 0. 5 software,17 kinds of test scheme were designed. The protein yield of each scheme was obtained,and Design expert 8. 0. 5 software was used to conduct variance analysis and interactive analysis among factors,to obtain the corresponding mathematical model. The results showed that in single factor experiments,the optimal time was 1 h,p H was 7. 8,and liquid-solid ratio was 15:1. The interactive analysis indicated that the optimal scheme for extracting SOD in Sedum aizoon was time of 0. 98 h,p H of 7. 79,liquid-solid ratio of 13. 71:1. By the method of pyrogallol autoxidation,the extracted SOD enzyme activity was measured,namely 12 U/g. The results could provide a reference for further utilization and research of Sedum aizoon resource.展开更多
Fingerprint analysis of Sedum aizoon L.was carried by High-performance liquid chromatography.There were 27 common peaks in the fingerprint of 10 samples.Eight of these common peaks,peaks 1(arbutin),4(gallic acid),10(m...Fingerprint analysis of Sedum aizoon L.was carried by High-performance liquid chromatography.There were 27 common peaks in the fingerprint of 10 samples.Eight of these common peaks,peaks 1(arbutin),4(gallic acid),10(myricetin-3-O-β-D-glucopyranoside),14(myricetin-3-O-α-L-rhamnopyranoside),19(quercetin-3-O-α-L-rhamnopyranoside),22(kaempferol-3-O-α-L-rhamnopyranoside),24(quercetin)and 26(kaempferol)were identified by reference substances.Moreover,six flavonoid compounds were simultaneously determined by the above method.By comparing with the constituents in the aerial parts,roots and flowers,it’s easy found that the aerial parts of S.aizoon had abundant flavonoids and the flavonoid aglycones mostly exist in the roots,while the flavonoid glycosides largely existing in the flowers.In addition,an UPLC-DAD-Q-TOF-MS method was developed for qualitative analysis of the aerial parts of S.aizoon.This study may provide a preliminary reference for the quality control of S.aizoon.展开更多
文摘This paper aimed to optimize extracting conditions of SOD in Sedum aizoon by response surface method. First,the single factor optimization tests were carried out. Three single factors were time,p H and liquid-solid ratio respectively. By Design expert 8. 0. 5 software,17 kinds of test scheme were designed. The protein yield of each scheme was obtained,and Design expert 8. 0. 5 software was used to conduct variance analysis and interactive analysis among factors,to obtain the corresponding mathematical model. The results showed that in single factor experiments,the optimal time was 1 h,p H was 7. 8,and liquid-solid ratio was 15:1. The interactive analysis indicated that the optimal scheme for extracting SOD in Sedum aizoon was time of 0. 98 h,p H of 7. 79,liquid-solid ratio of 13. 71:1. By the method of pyrogallol autoxidation,the extracted SOD enzyme activity was measured,namely 12 U/g. The results could provide a reference for further utilization and research of Sedum aizoon resource.
基金The work was supported by the Natural Science Foundation Guidance Project of Liaoning(2018055455)the Young and Middle-aged Key Talent Training Program of Shenyang Pharmaceutical Universitythe Foundation of Education Department of Liaoning Province(2017LQN09).
文摘Fingerprint analysis of Sedum aizoon L.was carried by High-performance liquid chromatography.There were 27 common peaks in the fingerprint of 10 samples.Eight of these common peaks,peaks 1(arbutin),4(gallic acid),10(myricetin-3-O-β-D-glucopyranoside),14(myricetin-3-O-α-L-rhamnopyranoside),19(quercetin-3-O-α-L-rhamnopyranoside),22(kaempferol-3-O-α-L-rhamnopyranoside),24(quercetin)and 26(kaempferol)were identified by reference substances.Moreover,six flavonoid compounds were simultaneously determined by the above method.By comparing with the constituents in the aerial parts,roots and flowers,it’s easy found that the aerial parts of S.aizoon had abundant flavonoids and the flavonoid aglycones mostly exist in the roots,while the flavonoid glycosides largely existing in the flowers.In addition,an UPLC-DAD-Q-TOF-MS method was developed for qualitative analysis of the aerial parts of S.aizoon.This study may provide a preliminary reference for the quality control of S.aizoon.
