Fingerprint analysis of Sedum aizoon L.was carried by High-performance liquid chromatography.There were 27 common peaks in the fingerprint of 10 samples.Eight of these common peaks,peaks 1(arbutin),4(gallic acid),10(m...Fingerprint analysis of Sedum aizoon L.was carried by High-performance liquid chromatography.There were 27 common peaks in the fingerprint of 10 samples.Eight of these common peaks,peaks 1(arbutin),4(gallic acid),10(myricetin-3-O-β-D-glucopyranoside),14(myricetin-3-O-α-L-rhamnopyranoside),19(quercetin-3-O-α-L-rhamnopyranoside),22(kaempferol-3-O-α-L-rhamnopyranoside),24(quercetin)and 26(kaempferol)were identified by reference substances.Moreover,six flavonoid compounds were simultaneously determined by the above method.By comparing with the constituents in the aerial parts,roots and flowers,it’s easy found that the aerial parts of S.aizoon had abundant flavonoids and the flavonoid aglycones mostly exist in the roots,while the flavonoid glycosides largely existing in the flowers.In addition,an UPLC-DAD-Q-TOF-MS method was developed for qualitative analysis of the aerial parts of S.aizoon.This study may provide a preliminary reference for the quality control of S.aizoon.展开更多
A traditional herb that is rich in active substances such as flavonoids, polyphenols and alkaloids, Sedum aizoon L . has antioxidant and preventive effects against chronic diseases. Circadian rhythm disorders are ofte...A traditional herb that is rich in active substances such as flavonoids, polyphenols and alkaloids, Sedum aizoon L . has antioxidant and preventive effects against chronic diseases. Circadian rhythm disorders are often accompanied by changes in intestinal flora, and flavonoids that can regulate intestinal flora may be an effective way to prevent and regulate circadian rhythm disorders. In this study, we established a humanized circadian rhythm mouse model to explore the regulatory mechanism of flavonoids from Sedum aizoon L .(FSAL) on circadian rhythm disorders in mice. The results after feeding FSAL for 4 weeks showed that FSAL improved the imbalance of intestinal microbial structure caused by circadian rhythm disorders, thus regulating alanine, aspartate and glutamate metabolism, histidine metabolism and other metabolites and substances related to metabolism. The analysis of liver transcriptome showed that FSAL significantly regulated retinol metabolism, peroxisome proliferator-activated receptors(PPARs) pathway, and hepatitis C pathway in mouse liver. Therefore, this study obtained the conclusion that FSAL can regulate intestinal flora, metabolites and liver genes to improve circadian rhythm disorders and maintain mouse health.展开更多
Botrytis cinerea is a plant pathogen,which affects the quality of fruits.However,chemical antifungal agents are extremely harmful to the environment,hence it’s critical to create a natural plant-derived antifungal ag...Botrytis cinerea is a plant pathogen,which affects the quality of fruits.However,chemical antifungal agents are extremely harmful to the environment,hence it’s critical to create a natural plant-derived antifungal agent to replace chemical antifungal agents.The study explored the antifungal mechanisms of flavonoids from Sedum aizoon L.(FSAL)against B.cinerea.In the study,cell membrane served as an important breakthrough point.The minimum inhibitory concentration(MIC)of FSAL against B.cinerea was investigated.The propidium iodide(PI)staining observation of B.cinerea was explored.The activities of crucial enzymes involved in the metabolism were studied as well as the phospholipid and fatty acid changes.The expression of genes related to the membrane lipid metabolism was also measured.The results showed that FSAL had a certain inhibitory effect on B.cinerea and the MIC was 1.500 mg/mL.FSAL disrupted the cell membrane of B.cinerea,which decreased the contents of glutathione and proline at a low level as well as the membrane phospholipids.Compared with the control group,the activities of crucial enzymes were decreased,and the expression of key genes was reduced.It indicated that FSAL inhibited B.cinerea by disrupting its cell membrane,offering the possibility of a natural plant-derived antifungal agent for the preservation of fruits.展开更多
In the present study,genetic diversity and genetic relationship of 11materials including red clover ( Trifolium pretense L.),white clover ( T.repen L.) and alsike clover ( T.hybridum L.) and leaf-type variants of whit...In the present study,genetic diversity and genetic relationship of 11materials including red clover ( Trifolium pretense L.),white clover ( T.repen L.) and alsike clover ( T.hybridum L.) and leaf-type variants of white clover were investigated by SRAP (sequence related amplified polymorphism).Forty SRAP primers were screened,and 792 reliable bands were amplified,among which 426 (53.8%) were polymorphic.The number of polymorphic bands per pair of primers ranged from 3 to 38 with an average of 10.6.SRAP cluster analysis showed that the similarity coefficients between white clover materials were from 0.465 to 0.997 with an average of 0.812,indicating that there was certain genetic diversity among white clover.Specific bands appeared in white clover variants,indicating that there were certain differences in DNA sequence between normal while clover plants and their leaf-type variants.The similarity coefficients between red clover materials were from 0.457 to 0.827 with an average of 0.597,indicating that the different red clover germplasms had genetic diversity at the DNA molecular level.The results also showed that the genetic relationship of the alsike clover with the red clover was closer than that with the white clover.This study shows that SRAP technique can be effectively used for the analysis on interspecific and intraspecific relationship,germplasm resource identification and genetic diversity of clover.展开更多
基金The work was supported by the Natural Science Foundation Guidance Project of Liaoning(2018055455)the Young and Middle-aged Key Talent Training Program of Shenyang Pharmaceutical Universitythe Foundation of Education Department of Liaoning Province(2017LQN09).
文摘Fingerprint analysis of Sedum aizoon L.was carried by High-performance liquid chromatography.There were 27 common peaks in the fingerprint of 10 samples.Eight of these common peaks,peaks 1(arbutin),4(gallic acid),10(myricetin-3-O-β-D-glucopyranoside),14(myricetin-3-O-α-L-rhamnopyranoside),19(quercetin-3-O-α-L-rhamnopyranoside),22(kaempferol-3-O-α-L-rhamnopyranoside),24(quercetin)and 26(kaempferol)were identified by reference substances.Moreover,six flavonoid compounds were simultaneously determined by the above method.By comparing with the constituents in the aerial parts,roots and flowers,it’s easy found that the aerial parts of S.aizoon had abundant flavonoids and the flavonoid aglycones mostly exist in the roots,while the flavonoid glycosides largely existing in the flowers.In addition,an UPLC-DAD-Q-TOF-MS method was developed for qualitative analysis of the aerial parts of S.aizoon.This study may provide a preliminary reference for the quality control of S.aizoon.
基金supported by Natural Science Foundation of Zhejiang Province[LY16C200003]Zhejiang Provincial Key Research and Development,Program[2020C02037].
文摘A traditional herb that is rich in active substances such as flavonoids, polyphenols and alkaloids, Sedum aizoon L . has antioxidant and preventive effects against chronic diseases. Circadian rhythm disorders are often accompanied by changes in intestinal flora, and flavonoids that can regulate intestinal flora may be an effective way to prevent and regulate circadian rhythm disorders. In this study, we established a humanized circadian rhythm mouse model to explore the regulatory mechanism of flavonoids from Sedum aizoon L .(FSAL) on circadian rhythm disorders in mice. The results after feeding FSAL for 4 weeks showed that FSAL improved the imbalance of intestinal microbial structure caused by circadian rhythm disorders, thus regulating alanine, aspartate and glutamate metabolism, histidine metabolism and other metabolites and substances related to metabolism. The analysis of liver transcriptome showed that FSAL significantly regulated retinol metabolism, peroxisome proliferator-activated receptors(PPARs) pathway, and hepatitis C pathway in mouse liver. Therefore, this study obtained the conclusion that FSAL can regulate intestinal flora, metabolites and liver genes to improve circadian rhythm disorders and maintain mouse health.
基金This research was kindly supported through funding from National Natural Science Foundation of China(31772030)Student Research and Innovation Program of Ningbo University(2021SRIP3607).
文摘Botrytis cinerea is a plant pathogen,which affects the quality of fruits.However,chemical antifungal agents are extremely harmful to the environment,hence it’s critical to create a natural plant-derived antifungal agent to replace chemical antifungal agents.The study explored the antifungal mechanisms of flavonoids from Sedum aizoon L.(FSAL)against B.cinerea.In the study,cell membrane served as an important breakthrough point.The minimum inhibitory concentration(MIC)of FSAL against B.cinerea was investigated.The propidium iodide(PI)staining observation of B.cinerea was explored.The activities of crucial enzymes involved in the metabolism were studied as well as the phospholipid and fatty acid changes.The expression of genes related to the membrane lipid metabolism was also measured.The results showed that FSAL had a certain inhibitory effect on B.cinerea and the MIC was 1.500 mg/mL.FSAL disrupted the cell membrane of B.cinerea,which decreased the contents of glutathione and proline at a low level as well as the membrane phospholipids.Compared with the control group,the activities of crucial enzymes were decreased,and the expression of key genes was reduced.It indicated that FSAL inhibited B.cinerea by disrupting its cell membrane,offering the possibility of a natural plant-derived antifungal agent for the preservation of fruits.
基金Supported by Innovation Project of Shandong Academy of Agricultural Sciences(CXGC2016A02-1)China Agriculture Research System-Green Manure(CARS-22)Young Talents Training Program of Shandong Academy of Agricultural Sciences(2018-2020)
文摘In the present study,genetic diversity and genetic relationship of 11materials including red clover ( Trifolium pretense L.),white clover ( T.repen L.) and alsike clover ( T.hybridum L.) and leaf-type variants of white clover were investigated by SRAP (sequence related amplified polymorphism).Forty SRAP primers were screened,and 792 reliable bands were amplified,among which 426 (53.8%) were polymorphic.The number of polymorphic bands per pair of primers ranged from 3 to 38 with an average of 10.6.SRAP cluster analysis showed that the similarity coefficients between white clover materials were from 0.465 to 0.997 with an average of 0.812,indicating that there was certain genetic diversity among white clover.Specific bands appeared in white clover variants,indicating that there were certain differences in DNA sequence between normal while clover plants and their leaf-type variants.The similarity coefficients between red clover materials were from 0.457 to 0.827 with an average of 0.597,indicating that the different red clover germplasms had genetic diversity at the DNA molecular level.The results also showed that the genetic relationship of the alsike clover with the red clover was closer than that with the white clover.This study shows that SRAP technique can be effectively used for the analysis on interspecific and intraspecific relationship,germplasm resource identification and genetic diversity of clover.
