Enhancer of Shoot Regeneration 2(ESR2)regulates pollen maturation and vitality in watermelon(Citrullus lanatus)

Watermelon(Citrullus lanatus)holds global significance as a fruit with high economic and nutritional value.Exploring the regulatory network of watermelon male reproductive development is crucial for developing male sterile materials and facilitating cross-breeding.Despite its importance,there is a lack of research on the regulation mechanism of male reproductive development in watermelon.In this study,we identified that ClESR2,a VIIIb subclass member in the APETALA2/Ethylene Responsive Factor(AP2/ERF)superfamily,was a key factor in pollen development.RNA insitu hybridization confirmed significantClESR2 expression in the tapetum and pollen during the later stage of anther development.The pollens of transgenic plants showed major defects in morphology and vitality at the late development stage.The RNA-seq and protein interaction assay confirmed that ClESR2 regulates pollen morphology and fertility by interacting with key genes involved in pollen development at both transcriptional and protein levels.These suggest that Enhancer of Shoot Regeneration 2(ESR2)plays an important role in pollen maturation and vitality.This study helps understand the male reproductive development of watermelon,providing a theoretical foundation for developing male sterile materials.

Earth vitality:An integrated framework for tracking Earth sustainability

The Anthropocene era is characterized by the escalating impact of human activities on the environment,as well as the increasingly complex interactions among various components of the Earth system.These factors greatly affect the Earth's evolutionary trajectory.Despite notable strides in sustainable development practices worldwide,it remains unclear to what extent we have achieved Earth sustainability.Consequently,there is a pressing need to enhance conceptual and methodological frameworks to measure sustainability progress accurately.To address this need,we developed an Earth Vitality Framework that aids in tracking the Earth sustainability progress by considering interactions between spheres,recognizing the equal relationship between humans and nature,and presenting a threshold scheme for all measures.We applied this framework at global and national scales to demonstrate its usefulness.Our findings reveal that the current Earth Vitality Index is 63.74,indicating that the Earth is in a"weak"vitality.Irrational social institutions,unsatisfactory life experiences and the poor state of the biosphere and hydrosphere have remarkably affected the Earth vitality.Additionally,inequality exists between high-income and low-income countries.Although most of the former exhibit poor human-nature interaction,all of them enjoy good human well-being,while the opposite is true for the latter.Finally,we summarize the challenges and possible options for enhancing the Earth vitality in terms of coping with spillover effects,tipping cascades,feedback,and heterogeneity.

Understanding Urban Vitality from the Economic and Human Activities Perspective:A Case Study of Chongqing,China

The literature on urban vitality tends to focus on the built environment.This paper argues that some important processes in shaping vitality may be overlooked without examining the intensity and diversity of economic and human activities.Using newly developed spatial big data and adopting the methods of multi-indicator measurement and spatial analysis methods,we analyzed the pattern of urban vitality in Chongqing,a provincial city in western China and,on this basis,evaluated the creation and maintenance of urban vitality from the economic and human activities perspective.Our findings indicate that the impacts of economic and human activities are positive and significant.Among the three intensity and diversity indicators,economic intensity and population density show an effect on urban vitality stronger than that of economic diversity.However,economic diversity has the strongest superposition or interactive effect,and is thus an important foundation dynamic.The positive effect of population density on urban vitality is largely a result of Chongqing’s jobs-housing balance.The case of Chongqing highlights the importance of topographic features,historical inheritance,large-scale migration,and cultural activities in shaping the distinctive vitality pattern of a city.This study contends that the creation and maintenance of urban vitality can not be fully explained without incorporating the impacts of economic and human activities.It contributes to a comprehensive measurement of urban vitality and enriches its connotations.

Improvement of the Vitality of Public Space under the Background of Urban Renewal

As an indispensable part of urban development,urban renewal plays an important role in terms of promoting urban society,economy,culture,etc.,and has a profound impact on the sustainable development of cities and the happiness index of residents.Public space,as an important part of a city,plays a more critical role in urban renewal.The vitality of public space not only reflects the degree of urban cultural inheritance,but also shows the level of urban economic development,so it is an important issue that must be paid attention to in urban renewal work.In this paper,starting from the concept of urban renewal,public space and vitality,the influencing factors and methods of studying the vitality of public space under the background of urban renewal,as well as its advantages and limitations,were discussed,and some strategies for enhancing the vitality of public space under the background of urban renewal were finally put forward.It is expected to provide some reference and practical significance for the research and practice of urban renewal and the enhancement of the vitality of public space.

Impact of human settlements quality on urban vitality based on multisource data:A case study of Shahekou District,Dalian,China

Urban geography has always been concerned about the influence of human settlements on urban vitality,but few studies reveal the influence of human settlements on urban vitality at a micro-scale.This paper analyzes the spatial distribution characteristics of human settlements’quality and urban vitality at the micro-scale using Geodetectors and geographic weighted regression to analyze the relationship between human settlements and urban vitality.The results are shown as follows:there is still a significant development space for human settlements quality in Shahekou District,with obvious spatial dependence characteristics and significant gaps between various systems;the urban vitality of Shahekou District has obvious timeliness,and the urban vitality undergoes significant changes over time,which is related to the human settlements quality.The spatial distribution presents a single core spatial distribution structure with strong relative stability.The spatial distribution of cold and hot spots shows a pattern of“high in the north and low in the south,high in the east and low in the west”,with an increasing trend from southwest to northeast;the reachability of public transport has a significant impact on urban vitality.Its synergy with other variables is the leading force forming the spatial distribution of urban vitality.The environmental system,support system and social system are the significant factors affecting the urban vitality of Shahekou District.

Data Collection and Research on the Vitality of Public Space in Traditional Villages: A Case Study of Cuandixia Village

The optimization and renewal of rural space is the foundation for building livable,business friendly and harmonious countryside.Three types of data on the spatial vitality of traditional villages are collected and analyzed.A street and alley axis map model is established using spatial syntax,and the degree of industry aggregation is quantified using POI data,and the results of syntactic calculation are validated with the help of popular review text preferences.Taking Cuandixia Village as an example,this paper found that its public space nodes have a pattern of one axis and scattered points;the street and alley space is well preserved but slightly lacking in transportation;the user group is single and the form is traditional.This paper could provide corresponding suggestions for stimulating the vitality of public spaces in traditional villages,in order to provide inspiration for the revitalization design of public spaces in traditional villages.

Research on Evaluation of Spatial Vitality and Influencing Factors of Guesthouses Public Areas Based on Rural Tourism Development

In recent years,China has continued to promote the strategy of rural revitalization.The rural tourism industry has developed rapidly in the context of rural revitalization.Now the rural B&B industry has become an important pillar in solving the problem of rural tourism accommodation.Most of the villages in China have begun to carry out the construction of rural lodging,for example,there are residents who use idle houses to carry out construction on their own initiative and projects developed by village collectives,so the rural lodging industry has been vigorously developed.The vitality of supporting industries and landscape resources around the distribution of rural lodging has become a hot spot of rural issues research in recent years.Therefore,we can use the method of data analysis to analyze the industry and function of the public area around the distribution of rural B&B.Through the comparison of data and the interpretation of related cases,the core elements affecting the spatial vitality of B&B public areas can be identified.We will analyze and improve the related elements to find a reasonable way to enhance the spatial vitality value.Finally,the article will put forward suggestions to improve the related spatial vitality as a way to promote the development of rural B&B industry and the progress of rural tourism.

Low Level of Cystic Fibrosis Transmembrane Conductance Regulator Is Associated with Human Sperm Autophagy and Vitality

Low sperm motility is one of the main causes of male infertility. Cystic fibrosis transmembrane conductance regulator (CFTR, an anion channel protein) is related to the progressive motility of sperm. CFTR disruptor CFTRinh-172 or forskolin (FSK) in this study were used to treat human sperm separately, and the rates of sperm autophagy and progressive motility, mitochondrial membrane potential (MMP) and ATP concentration, and the expression levels of related factors were detected to explore their relationship. It was showed that sperms treated with CFTRinh-172 or FSK reduced the levels of cAMP, CFTR and PKA, but increased sperm autophagy rate, expression levels of AMPK and LC3B. However, reactive oxygen species content had no significant difference. It was indicated that low level of CFTR performed with cAMP and its downstream effectors such as PKA and AMPK to regulate mitochondrial structure and function, leading to increased autophagy rate and reduced vitality of sperm.

Analysis of Landscape Vitality of Historical and Cultural Blocks Based on AHPFuzzy Comprehensive Evaluation Method:A Case Study of Daopashi Street in Anqing City

Historical and cultural blocks are witnesses of history and inheritors of culture. As one of the main spaces for outdoor interaction in historical and cultural blocks, the improvement of its vitality is of great significance for the improvement of residential environment and the better inheritance of history and culture. Taking Daopashi Street in Anqing City as an example, an evaluation model of landscape spatial vitality of historical and cultural blocks was constructed from three aspects of viewing function, store status and service facilities, and analytic hierarchy process was used to determine the index weight and vaguely evaluate the landscape spatial vitality of historical and cultural blocks. The results show that through the comparison of weight, architectural style(0.317), the practicability of service facilities(0.168) and plant landscape(0.165) had a significant impact on the landscape spatial vitality of historical and cultural blocks,and the landscape spatial vitality of historical and cultural blocks in Daopashi Street in Anqing City was at a good level.

Current Status of Research on Pollen Vitality and Stigma Receptivity of Wuren Walnut(Juglans sigillata)in Guizhou Province

Wuren walnut is a unique germplasm resource of Juglans sigillata in Guizhou Province,named after its purple and black seed coat.It is a typical medicinal and edible plant with high nutritional value and good taste compared with ordinary walnut.The color of its inner seed coat is different from that of ordinary walnut,which is mostly light yellow.Wuren walnut has great market potential for development.To lay a theoretical foundation for the future breeding of improved varieties,pollination tree configuration,and hybrid breeding of Wuren walnut in Guizhou Province,this paper summarized the current research status of pollen vitality and stigma acceptability of Guizhou Wuren walnut in recent years,and looked forward to future research and application.

生命体征智能采录系统在监护患者护理中的应用研究

目的探索生命体征智能采录系统在监护患者护理中的应用效果,用以解决原有手工抄录生命体征数据带来的效率低下、误差率高问题,推进全院一体化生命体征数据监测中心的建立。方法利用设备集成模块,将各类生命体征测量设备统一接入中心监护系统,中心监护系统将接收到的生命体征数据进行统一化、标准化和智能化处理,再通过数据网关转发数据至医院各业务系统,完成生命体征智能采录系统的设计,实现医疗设备应用过程中,采集数据的自动化传输、智能化存储和标准化应用。选取5个普通病房和5个重症监护室的生命体征数据采集时间及采录准确性、护理人员满意度作为评价指标对生命体征智能采录系统的效果进行分析。结果生命体征智能采录系统应用后,普通病房及重症病房各科室的生命体征数据采录时间均较系统应用前显著降低,每天平均节省时间分别为14.14~72.59 h、17.00~81.70 h;数据采录准确性和护理人员满意度均高于系统应用前,且差异均有统计学意义(P<0.05)。结论生命体征智能采录系统的应用,实现了生命体征测量设备互联、数据互通、生命体征数据采集流程的数字化,提升了护理安全和护理质量,可帮助医生动态监测患者病情变化并作出准确诊断。

中国式现代化:活力与秩序的辩证统一

活力与秩序是现代化的两个关键要素,二者既是现代社会的重要特征,也是实现现代化的重要支撑。活力以自主探索开拓创新为建构取向,秩序以人民至上为价值内核;活力是社会发展的动力机制,秩序是社会发展的平衡机制。中国式现代化较好地协调了动力机制与平衡机制。人民创造活力充分迸发、改革创新活力全面激发、精神文化活力持续焕发,为中国式现代化提供了强劲动力;经济发展安全有序、民主政治稳定有序、精神文化健康有序、社会治理和谐有序、生态环境优美有序,为中国式现代化提供了重要支撑。中国式现代化是一个系统工程,在新时代新征程上,要更好地把握活力与秩序的辩证统一关系,保持活力与秩序的动态平衡,确保中国式现代化行稳致远。

Why Teaching and Research Activities Lack Vitality?:A Probe into the Basic Psychological Needs of Teachers

Teaching and research activities are of great significance to the professional development of teachers.The basic psychological needs of teachers include autonomy,competence,and attribution.The engagement of teachers in teaching and research activities can be analyzed from these three perspectives.Under the management system of teaching and research,teachers generally lack autonomy in the organization of teaching and research activities;they are also lack scientific research knowledge and ability;in addition,under the influence of harmonious interpersonal relationships and the culture of individualism,there is a dearth in real communication among teachers.In light of this,we propose targeted countermeasures to promote the vitality of teaching and research activities.

超声引导下竖脊肌平面阻滞对多发肋骨骨折患者术后早期呼吸功能的影响

目的探讨超声引导下竖脊肌平面阻滞(ESPB)对多发肋骨骨折(MRFs)患者术后早期呼吸功能和炎性因子的影响。方法选择2019年2月至2021年12月择期行多发肋骨骨折手术患者58例,男42例,女16例,年龄18~64岁,BMI 18.5~30.0 kg/m^(2),ASAⅠ或Ⅱ级。采用随机数字表法将患者分为两组:ESPB联合全身麻醉组(E组)和单纯全身麻醉组(G组),每组29例。E组于麻醉诱导后在侧卧位下实施超声引导下ESPB,给予0.5%罗哌卡因0.4 ml/kg。记录麻醉诱导前、出PACU时、术后24、48 h的用力肺活量(FVC)、动脉血气分析、静息和咳嗽时VAS疼痛评分。记录术后0~24 h、24~48 h的PCIA有效按压次数和补救镇痛情况。记录麻醉诱导前、术后24、48 h的IL-6和TNF-α浓度。结果与G组比较,E组出PACU时、术后24、48 h的FVC明显增大(P<0.05),PaCO_(2)、静息和咳嗽时VAS疼痛评分明显降低(P<0.05)。与G组比较,E组术后0~24 h、24~48 h的PCIA有效按压次数和补救镇痛率明显降低(P<0.05)。与G组比较,E组术后24、48 h的IL-6和TNF-α浓度明显降低(P<0.05)。结论超声引导下ESPB可为MRFs患者提供良好的术后镇痛,促进术后早期呼吸功能的恢复。

数字科技赋能乡村振兴的作用机理·现实挑战与实现路径

伴随着我国乡村振兴战略的全面推进,数字科技加速向农业农村各领域渗透应用,为加快农业农村现代化、实现乡村振兴提供重大机遇。但是,乡村振兴是一个系统工程,目前现有研究中数字科技对整体乡村振兴的作用机制缺乏理论阐释,由此可能造成夸大或者弱化数字科技在乡村振兴中作用的后果,影响数字科技、乡村振兴、数字乡村等相关政策的制订。在梳理数字科技概念与研究边界的基础上,分析了数字科技的4个发展特征,结合乡村振兴的实现目标,理论阐述了数字科技推进乡村振兴的作用机理,剖析了以数字科技赋能乡村振兴的现实挑战,在此基础上提出了数字乡村背景下数字科技促进乡村振兴建设的实现路径。

沿海省份城市发展活力评价及其互动关系研究——以副省级城市为例

以德鲁克社会创新为理论基础,基于城市内三大行为活动主体构建包含人居环境、营商环境、治理环境系统的发展活力评价指标体系,利用多因子分层动态激励模型对2006—2020年我国沿海省份8个副省级城市的发展活力进行评价,运用耦合协调模型和面板向量自回归模型分别测度并分析3个子系统间的耦合协调情况和互动关系。结果表明:(1)各城市的发展活力由高到低依次为广州、深圳、杭州、南京、青岛、宁波、大连、厦门,城市间发展活力综合评价结果两极分化明显,营商环境是影响城市发展活力提升最重要的因素,人居环境次之,治理环境最小。(2)8个城市的耦合协调级别不高,整体处于失调类型。(3)3个系统间相互作用,但其发展水平主要受自身影响。其中,人居环境系统显著受其他两个系统影响;营商环境系统受治理环境系统影响较强;治理环境系统主要依靠自身调节,受其他系统的影响较小。

金融科技能提升城市包容性绿色全要素生产率吗?

包容性绿色全要素生产率强调绿色发展、社会公平与经济增长协调并进,是判断城市经济发展质量的重要依据。本文以2011—2019年中国284个地级及以上城市的数据为样本,实证检验了金融科技对城市包容性绿色全要素生产率的影响及作用机制。研究发现,金融科技对城市包容性绿色全要素生产率具有明显的促进作用,主要表现在技术进步指数显著提升,这一结论在经过一系列内生性处理和稳健性检验后依然成立。机制分析显示,金融科技能够通过促进绿色创新、增加环保投资以及激发创业活力提升城市包容性绿色全要素生产率。异质性分析表明,金融科技对包容性绿色全要素生产率的提升作用在人力资本水平、金融发展水平和基础设施水平较高的城市中更为明显。本文的研究为加快实现经济绿色发展和包容性增长的“双重红利”提供了重要的经验证据。

城市创新创业活力与新兴产业核心技术:空间极化、规模门槛与中介效应

城市新企业、新资本和新发明涌现是孕育新兴产业核心技术的活力源泉,对加快形成新质生产力具有重要意义。利用中国城市面板数据考察创新创业活力对新兴产业核心技术的影响及作用机制。研究发现:(1)城市创新创业活力显著促进新兴产业核心技术数量提升,在考虑一系列稳健性检验后结论仍然成立。(2)创新创业活力对新兴产业核心技术活动的影响存在“边际递增”的城市规模门槛特征,而对邻近城市产生显著的抑制作用,存在“马太效应”和“虹吸效应”。(3)城市创新创业活力对新兴产业核心技术活动的影响存在区域和梯度异质性,不同地理位置和文化区的城市存在显著差异,而资源型城市、智慧城市和创新型城市的分组回归体现了梯度性特征。(4)创新创业活力通过提升城市多样性,特别是专业化部门的多样性程度,促进新兴产业核心技术数量增长。

城乡融合发展背景下浙江省未来乡村建设及其推进机制

在国家乡村振兴、新型城镇化及城乡融合发展战略下,浙江省率先探索未来乡村建设路径,其本质是新时代乡村建设的重要成果。作为浙江省共同富裕现代化创建单元之一,未来乡村重点围绕人本化、生态化、数字化三大价值理念,对乡村九大场景建设营造提出具体要求,并集成共富乡村、美丽乡村、数字乡村、人文乡村、善治乡村于一体。在未来乡村建设实践层面,浙江省通过“地方探索—试点创建—建成提升—全面推广”的实施路径,省市县三级联动的申报创建评价流程体系以及各个政府部门的职责作用,逐步形成“千村引领、万村振兴、全域共富、城乡和美”的乡村建设格局,以全面推动乡村振兴、宜居宜业和美乡村建设和城乡融合发展。

中国式现代化进程中产业数字化的就业效应研究

产业数字化是数字经济的重要组成部分,在加速推进中国式现代化的过程中,数字经济对于促进就业增长发挥着越来越重要的作用。利用2006—2019年中国265个城市数据和企业信息数据,实证检验了产业数字化的就业效应和影响机制。研究发现,产业数字化可以有效促进就业,并主要通过产业结构升级、提升创业活跃度促进就业,但提高生产效率也会抑制就业。异质性分析表明,在制造业、采矿业等从事简单重复和危险工种的劳动力,往往会受到产业数字化发展的负面影响,就业机会减少。进一步研究发现,产业数字化能够显著提升就业质量,优化就业结构。因此,建议加快数字经济发展,促进数字经济和实体经济融合;加快传统产业结构升级改造,拓展产业链的长度、宽度和深度,创造更多就业岗位;提高城市创新能力,提升创业活跃度,通过创新创业带动就业。