Seed Priming Improves Enzymatic and Biochemical Performances of Rice During Seed Germination under Low and High Temperatures

As an abiotic stress,adverse germination temperatures cause serious disruptions in physiological and biochemical processes involved in seed germination.Using a factorial experiment,we examined the effects of different seed priming treatments on enzymatic and biochemical performances of rice seed germination under different temperatures.Each of the rice genotypes(Hashemi,Sadry-domsefid,IRON-70-7053-7 and NORIN-22)was primed with hydro-hardening,KCl,CaCl2 and ascorbic acid(AsA)and without a priming agent as a control at low(15℃),optimum(25℃)and high(35℃)germination temperatures.The results showed that the enzymatic and biochemical performances of all the rice genotypes were affected by the seed priming agents,especially under the low germination temperature.At 15℃,seed priming with AsA was found to be the best agent for the activities of amylase,α-amylase,catalase(CAT),peroxidase(POX),ascorbate peroxidase(APOX)and superoxide dismutase(SOD)as well as the content of soluble sugars in the NORIN-22 genotype,and for protease activity and soluble protein content in the IRON-70-7053-7 genotype.SOD at the low germination temperature and CAT,POX and protease at the optimum and high germination temperatures were the most important enzymes in occurrence of germination potential in terms of seedling length,vigor index,normal seedling rate and germination rate.Under the priming agents,the highest changes in normal seedling rate were observed at the low and optimum germination temperatures by AsA priming in the Hashemi and NORIN-22 genotypes,and at the high germination temperature under KCl priming in the Hashemi genotype.

Chitosan Seed Priming Improves the Yield and the Root System of Faba Bean(Vicia faba L.)Short Title:Chitosan Impact on Yield of Faba Bean

Faba bean is an important legume crop with food and feed applications.Since 2015,the culture of faba bean has decreased in France.Poor yield caused by climate change is proposed as one of the main reasons.Chitosan is a promising biopolymer with empirically proved impact on germination and disease tolerance of faba bean.However,the impact of chitosan seed priming on germination and yield of this plant is not experimentally studied.We followed chitosan primed faba bean seeds,from sowing until maturation,using germination,fruit and grain parameters.We observed that chitosan seed priming ameliorated several yield related traits.Germination rate,length of radicle and seedling,radicle dry matter,number of pods/plants,number of grains/pods were increased in chitosan primed faba beans.Total grain mass/plant increased up to 47%in treated plants.The probable underlying mechanisms,namely,root enhancement nitrogen metabolism,and elicitation of immune system are discussed.We conclude that,chitosan seed priming with compatible commercial preparations could be a potential solution to recompensate the loss of yield under variable climate conditions.

Mepiquat chloride priming confers the ability of cotton seed to tolerate salt by promoting ABA-operated GABA signaling control of the ascorbate–glutathione cycle

Background Ensuring that seeds germinate and emerge normally is a prerequisite for cotton production,esp.in areas with salinized soil.Priming with mepiquat chloride(MC)can promote seed germination and root growth under salt stress,but its mechanism has not been fully elucidated.In this study,physiological and biochemical experiments revealed that MC-priming promotes the tolerance of cotton seeds to salt stress by increasing the ability of antioxidant enzymes related to the ascorbate-glutathione(AsA-GSH)cycle to scavenge reactive oxygen species(ROS).Results Results revealed that treatment with inhibitors of abscisic acid(ABA)and γ-aminobutyric acid(GABA)biosynthesis reduced the positive effects of MC-priming.Similarly,MC-priming increased the contents of ABA and GABA under salt stress by stimulating the expression levels of GhNCED2 and GhGAD4 and the activity of calmodulin-binding(CML)glutamate decarboxylase(GAD).Further analysis showed that an inhibitor of ABA synthesis reduced the positive impacts of MC-priming on the content of GABA under salt stress,but the content of ABA was not affected by the GABA synthesis inhibitor.Furthermore,a multi-omics analysis revealed that MC-priming increased the abundance and phosphorylation levels of the proteins related to ABA signaling,CML,and Ca^(2+)channels/transporters in the MC-primed treatments,which resulted in increased oscillations in Ca^(2+)in the MC-primed cotton seeds under salt stress.Conclusion In summary,these results demonstrate that MC-mediated ABA signaling operates upstream of the GABA synthesis generated by GAD by activating the oscillations of Ca^(2+)and then enhancing activity of the AsA-GSH cycle,which ensures that cotton seeds are tolerant to salt stress.

Early ABA-stimulated maintenance of Cl^(−) homeostasis by mepiquat chloride priming confers salt tolerance in cotton seeds

Mepiquat chloride(MC)priming alleviates the effects of salt stress during seed germination in cotton(Gossypium hirsutum L.),but the mechanisms underlying its effects are unknown.We found that MC priming increases salt tolerance,as evidenced by marked increases in seed vigor and germination rates,and alleviated salt toxicity by reducing Cl^(−)accumulation in germinating seeds.Consistently,electrophysiological experiments revealed that the seeds with MC priming displayed superior Cl^(−)exclusion ability in the root apex.These beneficial effects of MC priming were abolished by the abscisic acid(ABA)-synthesis blocker fluridone under salt stress.MC priming induced an early response to acclimatization and stress,as indicated by rapidly increasing ABA content during initial exposure to salt stress.Transcriptome analyses revealed that MC priming induced an array of differentially expressed genes(DEGs)in germinating seeds.The most noticeable changes in germinating seeds were MC priming-induced increases in the expression of DEGs encoding components of ABA biosynthesis,ABA catabolism,and ABA signaling pathways under salt stress.MC priming also increased the expression of some DEGs encoding Cl^(−)ion transporters(e.g.CCC,SLAC1/SLAH1/SLAH3,CLC,and ALMT9)in germinating seeds.These results indicate that MC priming-induced ABA contributes to Cl^(−)homeostasis in tissues and acts as a positive regulator of salt tolerance via regulation of Cl^(−)transporters(particularly CCC and SLAC1/SLAH1/SLAH3).Taken together,these findings shed light on the molecular mechanism underlying MC-mediated tolerance to salt stress during seed germination.

Effect of Dates and Methods of Sowing with and without Hydropriming on Growth,Phenology and Yield of Sorghum under Semi Arid Conditions of Eritrea

A study was carried out at the research farm of Hamelmalo Agricultural College during summer 2015 to study the effect of dates and methods of sowing with and without hydro-priming on growth,development and yield of sorghum.The experiment consisted of 2 factors-three dates of sowing viz.D0(Sowing before the onset of first summer rain,June 30),D1(Sowing with the onset of first summer rains,July 7)and D2(Sowing after the onset of summer rains,July 14)and three methods of sowing viz.SP(Direct sowing with hydro seed priming),TR(Transplanting)and DS(Direct sowing without hydro seed priming).The experiment was conducted in split plot design with 4 replications by keeping dates of sowing in the main plots and methods of sowing in the sub plots.The data on growth parameters,crop phenology,yield components and yield were recorded.The data were analysed using GENSTAT software and inferences were drawn by using LSD at 5%level of significance.Sowing methods significantly affected leaf area and leaf area index but sowing dates and their interaction with sowing methods did not show significant difference.Stand count,plant height,phenological parameters,thousand grain weight,grain and biomass yield were significantly affected by the sowing dates and methods but no significant difference in their interaction on all the parameters except in stand count and phenological parameters.Sowing before the onset of first summer rains(D0)resulted in significantly highest grain yield.Among the sowing methods,transplanting resulted in significantly higher grain yield seconded by direct sowing with hydro seed priming.Transplanting is less profitable and practicable as it is more laborious and difficult to apply in larger areas.

Arsenic Accumulation in Rice:Sources,Human Health Impact and Probable Mitigation Approaches

The human body loading with arsenic(As)through rice consumption is a global health concern.There is a crucial need to limit As build-up in rice,either by remediating As accumulation in soils or reducing As levels in irrigation water.Several conventional approaches have been utilized to alleviate the As accumulation in rice.However,except for some irrigation practices,those approaches success and the adoption rate are not remarkable.This review presents human health risks posed due to consumption of As contaminated rice,evaluates different biomarkers for tracing As loading in the human body,and discusses the latest advancement in As reducing technologies emphasizing the application of seed priming,nanotechnology,and biochar application for limiting As loading in rice grains.We also evaluate different irrigation techniques to reduce As accumulation in rice.Altering water management regimes significantly reduces grain As accumulation.Bio-and nano-priming of rice seeds improve germination and minimize As translocation in rice tissues by protecting cell membrane,building pool around seed coat,methylation and volatilization,or quenching harmful effects of reactive oxygen species.Nanoparticle application in the form of nano-adsorbents or nano-fertilizers facilitates nano-remediation of As through the formation of Fe plaque or sorption or oxidation process.Incorporating biochar in the rice fields significantly reduces As through immobilization,physical adsorption,or surface complexation.In conclusion,As content in cooked rice depends on irrigation source and raw rice As level.