The promoter region (BCSP666) of b-conglycinin a-subunit gene from the genomic DNA of soybean Jilin 43 was isolatedby PCR method. Sequencing analysis showed that the cloned fragment BCSP666 had the similar structure ...The promoter region (BCSP666) of b-conglycinin a-subunit gene from the genomic DNA of soybean Jilin 43 was isolatedby PCR method. Sequencing analysis showed that the cloned fragment BCSP666 had the similar structure to the soybeanseed-specific promoter b-conglycinin a'-subunit gene promoter and b-conglycinin b-subunit gene promoter, and it alsocontains many motifs that contribute to the seed-specific promoter activity. Based on this sequencing analysis, wededuced that promoter fragment BCSP666 had the seed-sepecific promoter activity. And then we constructed the seed-specific expression vector pBMI666 with the promoter fragment BCSP666 and D6-fatty acid desaturase gene fromMortierella isabellina. The D6-fatty acid desaturase is the rate-limiting enzyme of the desaturation of linoleic acid in theproduction of a human essential fatty acid, g-linolenic acid(GLA). The production of g-linolenic acid(GLA) was observedin soybean callus cells, which were transformed with this vector. This confirmed the activity of the activity fragmentBCSP666.展开更多
The upstream regulatory region of a seed specific gene was isolated from the genomic DNA of Brassica napus by PCR amplification. The cloned fragment contained 1755 nucleotides, and shared a sequence homology of 99.6%...The upstream regulatory region of a seed specific gene was isolated from the genomic DNA of Brassica napus by PCR amplification. The cloned fragment contained 1755 nucleotides, and shared a sequence homology of 99.6% with the reported data. The coding region of oleic acid desaturase gene was then cloned from Arabidopsis thaliana. The sequencing analysis indicated that the sequence of the PCR product was just the same as reported before. In addition, the plant expression vector harboring the seed specific promoter and trans Fad2 gene was constructed.展开更多
[Objectives]The effect and mechanism of grape seed and sweet wormwood extract on growth of Monopterus albus were studied.[Methods]1 500 healthy M.albus with average mass of 23.8 g/tail were randomly divided into 5 gro...[Objectives]The effect and mechanism of grape seed and sweet wormwood extract on growth of Monopterus albus were studied.[Methods]1 500 healthy M.albus with average mass of 23.8 g/tail were randomly divided into 5 groups.The control group was fed with basal diet and the experimental groups were fed with 10,20,30 and 40 g/kg of grape seed and sweet wormwood extract,respectively.The growth performance of the fish was measured after 60 d of feeding at 23-28℃.[Results]The addition of grape seed and sweet wormwood extract in basal diet could increase the body weight gain rate,specific growth rate and protein efficiency,and decrease the feed coefficient.The activity of digestive enzymes,glutamic-oxaloacetic transaminase,glutamic-pyruvic transaminase,blood alkaline phosphatase and serum total protein was increased,and the contents of blood glucose and triglyceride were decreased.Compared with the control group,the above indexes showed significant difference(P<0.05)when the dosage was 20 and 30 g/kg(P<0.05).The difference was not significant(P>0.05)when the dosage was 10 and 40 g/kg(P>0.05).[Conclusions]As a feed additive,the plant extract from grape seed and sweet wormwood could increase digestive enzyme activity and regulate blood biochemical parameters of fish body.It had the function of promoting the digestion and absorption,enhancing the metabolic activity of 3 main nutrients,and hastening the growth of fish.展开更多
As the main byproduct of cotton fiber,the cotton seed yields about 1.6 times that of fiber,with its oil rich in unsaturated fatty acids,mainly linoleic acid.It is desirable for breeders to increase the oil content of ...As the main byproduct of cotton fiber,the cotton seed yields about 1.6 times that of fiber,with its oil rich in unsaturated fatty acids,mainly linoleic acid.It is desirable for breeders to increase the oil content of cottonseed without affecting the yield and quality of cotton fiber.In this study,a seed-specific promoter-(alpha-globulin gene promoter-)driven GhDGAT1 overexpression vector(P_(aGlob)-GhDGAT1)was constructed and used to transform an upland cotton line YZ1(Gossypium hirsutum).Overexpression of the cotton gene GhDGAT1 in cotton seeds increased its total oil content from 4.7%to 13.9%in different transgenic lines and different generations.With the increase of oil content,the composition and contents of the main fatty acids in cotton seed also changed,as reflected by the contents of the main saturated fatty acids and unsaturated oleic acid.GhDGAT1 could be used to increase oil content and improve oil composition in cottonseed.展开更多
Chemical vapor deposition (CVD) is the most efficient method to grow large-area two dimensional (2D) transition metal dichiacogenides (TMDCs) in high quality.Monolayer molybdenum disulfide (MoS2) and seed-assistant ar...Chemical vapor deposition (CVD) is the most efficient method to grow large-area two dimensional (2D) transition metal dichiacogenides (TMDCs) in high quality.Monolayer molybdenum disulfide (MoS2) and seed-assistant are the mostly selected 2D TMDC and growth strategy for such CVD processes,respectively.Though the advantages of seed catalysts in facilitating the nucleation,achieving higher yield and better repeatability,as well as their effects on the morphologies of as-grown MoS2 have been studied,the influence of seeding promoters on both optical and electrical properties of as-grown monolayer MoS2 is not known comprehensively,which is indeed critical for understanding fundamental physics and developing practical application of such emerging 2D semiconductors.In this report,we systematically investigated the effect of different seeding promoters on the properties of CVD-grown monolayer MoS2.It is found that different seed molecules lead to different impacts on the optical and electrical properties of as-grown monolayer MoS2.Among three different seed catalysts (perylene-3,4,9,10-tetracarboxylic acid tetrapotassium salt (PTAS),copper phthalocyanine (CuPc),and crystal violet (CV)),PTAS performs better in obtaining large area monolayer MoS2 with good optical quality and high electrical mobility than the other two.Our work gives a guide for modifying the properties of as-grown monolayer MoS2 and other 2D transition metal dichalcogenides in seeding promoters-assisted synthesis process.展开更多
基金supported by the National Natural Science Foundation of China(30200176)the Key Foundation of Tianjin of China(013802511)
文摘The promoter region (BCSP666) of b-conglycinin a-subunit gene from the genomic DNA of soybean Jilin 43 was isolatedby PCR method. Sequencing analysis showed that the cloned fragment BCSP666 had the similar structure to the soybeanseed-specific promoter b-conglycinin a'-subunit gene promoter and b-conglycinin b-subunit gene promoter, and it alsocontains many motifs that contribute to the seed-specific promoter activity. Based on this sequencing analysis, wededuced that promoter fragment BCSP666 had the seed-sepecific promoter activity. And then we constructed the seed-specific expression vector pBMI666 with the promoter fragment BCSP666 and D6-fatty acid desaturase gene fromMortierella isabellina. The D6-fatty acid desaturase is the rate-limiting enzyme of the desaturation of linoleic acid in theproduction of a human essential fatty acid, g-linolenic acid(GLA). The production of g-linolenic acid(GLA) was observedin soybean callus cells, which were transformed with this vector. This confirmed the activity of the activity fragmentBCSP666.
文摘The upstream regulatory region of a seed specific gene was isolated from the genomic DNA of Brassica napus by PCR amplification. The cloned fragment contained 1755 nucleotides, and shared a sequence homology of 99.6% with the reported data. The coding region of oleic acid desaturase gene was then cloned from Arabidopsis thaliana. The sequencing analysis indicated that the sequence of the PCR product was just the same as reported before. In addition, the plant expression vector harboring the seed specific promoter and trans Fad2 gene was constructed.
基金Supported by Hunan Science and Technology Department Planning Project(2012NK3056)
文摘[Objectives]The effect and mechanism of grape seed and sweet wormwood extract on growth of Monopterus albus were studied.[Methods]1 500 healthy M.albus with average mass of 23.8 g/tail were randomly divided into 5 groups.The control group was fed with basal diet and the experimental groups were fed with 10,20,30 and 40 g/kg of grape seed and sweet wormwood extract,respectively.The growth performance of the fish was measured after 60 d of feeding at 23-28℃.[Results]The addition of grape seed and sweet wormwood extract in basal diet could increase the body weight gain rate,specific growth rate and protein efficiency,and decrease the feed coefficient.The activity of digestive enzymes,glutamic-oxaloacetic transaminase,glutamic-pyruvic transaminase,blood alkaline phosphatase and serum total protein was increased,and the contents of blood glucose and triglyceride were decreased.Compared with the control group,the above indexes showed significant difference(P<0.05)when the dosage was 20 and 30 g/kg(P<0.05).The difference was not significant(P>0.05)when the dosage was 10 and 40 g/kg(P>0.05).[Conclusions]As a feed additive,the plant extract from grape seed and sweet wormwood could increase digestive enzyme activity and regulate blood biochemical parameters of fish body.It had the function of promoting the digestion and absorption,enhancing the metabolic activity of 3 main nutrients,and hastening the growth of fish.
基金This work was supported by National Science and Technology Major Project of the Ministry of Science and Technology of China(2016ZX08005-005).
文摘As the main byproduct of cotton fiber,the cotton seed yields about 1.6 times that of fiber,with its oil rich in unsaturated fatty acids,mainly linoleic acid.It is desirable for breeders to increase the oil content of cottonseed without affecting the yield and quality of cotton fiber.In this study,a seed-specific promoter-(alpha-globulin gene promoter-)driven GhDGAT1 overexpression vector(P_(aGlob)-GhDGAT1)was constructed and used to transform an upland cotton line YZ1(Gossypium hirsutum).Overexpression of the cotton gene GhDGAT1 in cotton seeds increased its total oil content from 4.7%to 13.9%in different transgenic lines and different generations.With the increase of oil content,the composition and contents of the main fatty acids in cotton seed also changed,as reflected by the contents of the main saturated fatty acids and unsaturated oleic acid.GhDGAT1 could be used to increase oil content and improve oil composition in cottonseed.
基金National Natural Science Foundation of China (Nos. 61774040, 61774042, and 51772317)National Young 1000 Talent Plan of China+1 种基金Shanghai Municipal Natural Science Foundation (Nos. 16ZR1402500, 16ZR1442700, and 17ZR1446500)Opening project of State Key Laboratory of Functional Materials for Informatics, Shanghai Institute of Microsystem and Information Technology Chinese Academy of Sciences, the National Key R&D program (No. 2017YFF0206106).
文摘Chemical vapor deposition (CVD) is the most efficient method to grow large-area two dimensional (2D) transition metal dichiacogenides (TMDCs) in high quality.Monolayer molybdenum disulfide (MoS2) and seed-assistant are the mostly selected 2D TMDC and growth strategy for such CVD processes,respectively.Though the advantages of seed catalysts in facilitating the nucleation,achieving higher yield and better repeatability,as well as their effects on the morphologies of as-grown MoS2 have been studied,the influence of seeding promoters on both optical and electrical properties of as-grown monolayer MoS2 is not known comprehensively,which is indeed critical for understanding fundamental physics and developing practical application of such emerging 2D semiconductors.In this report,we systematically investigated the effect of different seeding promoters on the properties of CVD-grown monolayer MoS2.It is found that different seed molecules lead to different impacts on the optical and electrical properties of as-grown monolayer MoS2.Among three different seed catalysts (perylene-3,4,9,10-tetracarboxylic acid tetrapotassium salt (PTAS),copper phthalocyanine (CuPc),and crystal violet (CV)),PTAS performs better in obtaining large area monolayer MoS2 with good optical quality and high electrical mobility than the other two.Our work gives a guide for modifying the properties of as-grown monolayer MoS2 and other 2D transition metal dichalcogenides in seeding promoters-assisted synthesis process.
