A simple and fast ultraviolet spectrophotometric method was used to determineselenium (Ⅳ) through the formation, separaion and quantitative determination of potassium butylxanthae -selenium complex. The complex ab...A simple and fast ultraviolet spectrophotometric method was used to determineselenium (Ⅳ) through the formation, separaion and quantitative determination of potassium butylxanthae -selenium complex. The complex absorbed light sharply at 375 nm. The optimum acidity ofthe complex formation was between pH 3.4 - 4.0. The absorption value was no change in 120 minutesafter the comlex was extracted by Carbon tetraChioride. The sensitivity of the colour reation was 0.2ng cm-2, The content of selenimp (Ⅳ) was within 0-30 μg and linear rclation was good,Y=0.07X＋0.025, relevent coefficient r＝0.9997. A great quantity of I, Cu, Co, the and SO4-2, PO4-3contained in .the Sample had no effect on the results ofdetenninion when seldsum was determined.When content of Fe was over l00gμg/ml and Zn was over 180μg/ml, there was diotance to the determination, but the disturbance was rem0ved by adding EDTA. When making use of the method todetermine containin seldsum additivef Cv =6.85, rate of recovery was 97.2%, wild jujubef;Cv=3.22,rate of recovery was 90. 1%, soil Samlef Cv=4.64, rate of recovery was 86.2%. The method for sensitivity measured was apprtoate to 2, 3-diamino naphthalene (DAN) fluorescence method.展开更多
文摘A simple and fast ultraviolet spectrophotometric method was used to determineselenium (Ⅳ) through the formation, separaion and quantitative determination of potassium butylxanthae -selenium complex. The complex absorbed light sharply at 375 nm. The optimum acidity ofthe complex formation was between pH 3.4 - 4.0. The absorption value was no change in 120 minutesafter the comlex was extracted by Carbon tetraChioride. The sensitivity of the colour reation was 0.2ng cm-2, The content of selenimp (Ⅳ) was within 0-30 μg and linear rclation was good,Y=0.07X＋0.025, relevent coefficient r＝0.9997. A great quantity of I, Cu, Co, the and SO4-2, PO4-3contained in .the Sample had no effect on the results ofdetenninion when seldsum was determined.When content of Fe was over l00gμg/ml and Zn was over 180μg/ml, there was diotance to the determination, but the disturbance was rem0ved by adding EDTA. When making use of the method todetermine containin seldsum additivef Cv =6.85, rate of recovery was 97.2%, wild jujubef;Cv=3.22,rate of recovery was 90. 1%, soil Samlef Cv=4.64, rate of recovery was 86.2%. The method for sensitivity measured was apprtoate to 2, 3-diamino naphthalene (DAN) fluorescence method.
