[Objective]The research aimed to construct deficient strain generated by Selenomonas ruminantium mutant with the acetic acid and analyze its fermentation characteristics.[Method]Based on the transposon tagging method,...[Objective]The research aimed to construct deficient strain generated by Selenomonas ruminantium mutant with the acetic acid and analyze its fermentation characteristics.[Method]Based on the transposon tagging method,Selenomonas ruminantium（recipient strain）was carried out the transposon mutagenesis via the transposon donor strain E.coli S17-1/pZJ25∷Tn5.The zygote was screened by using the selective medium which included kanamycin and sodium fluoroacetate.[Result]Seven transposon engineered strains which had the stable resistance to kanamycin and fluoroethanoic acid were screened.Selenomonas ruminantium mutant was carried out 16S rRNA and Tn5 PCR identification.Moreover,the specific activities of AK and PTA were analyzed.The mutant belonged to fluoroethanoic acid resistance strain with pta gene deficiency.[Conclusion]The research laid the foundation for further studying the cellular metabolic network and regulation of acetic acid in rumen microorganism of ruminant animal.展开更多
OBJECTIVE:To objectively reveal the relationship between tongue coating microbes and bitter taste,sticky and greasy taste in chronic atrophic gastritis(CAG)patients.METHODS:16S rRNA high-throughput sequencing was used...OBJECTIVE:To objectively reveal the relationship between tongue coating microbes and bitter taste,sticky and greasy taste in chronic atrophic gastritis(CAG)patients.METHODS:16S rRNA high-throughput sequencing was used to detect bacterial diversity and community composition of tongue coating microbes from samples of CAG patients.LEfSe algorithm was used for discovering the different tongue coating microbes in CAG patients with or without bitter taste,also that in CAG patients with or without sticky and greasy taste.RESULTS:We respectively compared the features of tongue coating microbes in bitter taste,sticky and greasy taste of CAG patients.At the genus level,25 tongue coating microbes were significantly different in CAG patients with bitter taste or without bitter taste;17 tongue coating microbes were significantly different in CAG patients with sticky and greasy taste or without sticky and greasy taste.Campylobacter and Rothia were closely related to CAG patients with bitter taste.Enterococcus,Serratia,Leptotrichia and Selenomonas were closely related to CAG patients with stick and greasy taste.CONCLUSION:Campylobacter and Rothia possibly contribute to bitter taste of CAG patients,and Enterococcus,Serratia,Leptotrichia and Selenomonas contribute to stick and greasy taste of CAG patients,which is potential for the diagnosis and treatment of CAG.展开更多
基金Supported by National Natural Science Fund Item(30230260,30600441)~~
文摘[Objective]The research aimed to construct deficient strain generated by Selenomonas ruminantium mutant with the acetic acid and analyze its fermentation characteristics.[Method]Based on the transposon tagging method,Selenomonas ruminantium（recipient strain）was carried out the transposon mutagenesis via the transposon donor strain E.coli S17-1/pZJ25∷Tn5.The zygote was screened by using the selective medium which included kanamycin and sodium fluoroacetate.[Result]Seven transposon engineered strains which had the stable resistance to kanamycin and fluoroethanoic acid were screened.Selenomonas ruminantium mutant was carried out 16S rRNA and Tn5 PCR identification.Moreover,the specific activities of AK and PTA were analyzed.The mutant belonged to fluoroethanoic acid resistance strain with pta gene deficiency.[Conclusion]The research laid the foundation for further studying the cellular metabolic network and regulation of acetic acid in rumen microorganism of ruminant animal.
基金Supported by Natural Science Foundation-funded Project:Diagnosis Model of Chronic Atrophic Gastritis with Damp-Heat Syndrome Based on Complex Biological Network Technology(No.81873236)Shanghai Key Laboratory:Health Identification and Assessment(No.21DZ2271000)。
文摘OBJECTIVE:To objectively reveal the relationship between tongue coating microbes and bitter taste,sticky and greasy taste in chronic atrophic gastritis(CAG)patients.METHODS:16S rRNA high-throughput sequencing was used to detect bacterial diversity and community composition of tongue coating microbes from samples of CAG patients.LEfSe algorithm was used for discovering the different tongue coating microbes in CAG patients with or without bitter taste,also that in CAG patients with or without sticky and greasy taste.RESULTS:We respectively compared the features of tongue coating microbes in bitter taste,sticky and greasy taste of CAG patients.At the genus level,25 tongue coating microbes were significantly different in CAG patients with bitter taste or without bitter taste;17 tongue coating microbes were significantly different in CAG patients with sticky and greasy taste or without sticky and greasy taste.Campylobacter and Rothia were closely related to CAG patients with bitter taste.Enterococcus,Serratia,Leptotrichia and Selenomonas were closely related to CAG patients with stick and greasy taste.CONCLUSION:Campylobacter and Rothia possibly contribute to bitter taste of CAG patients,and Enterococcus,Serratia,Leptotrichia and Selenomonas contribute to stick and greasy taste of CAG patients,which is potential for the diagnosis and treatment of CAG.
