Objective:To study the therapeutic effect of Huoxue Tongluo Decoction(HXTLD)on erectile dysfunction caused by ischemic stroke and identify the mechanisms involved.Methods:Network pharmacology was used to predict the k...Objective:To study the therapeutic effect of Huoxue Tongluo Decoction(HXTLD)on erectile dysfunction caused by ischemic stroke and identify the mechanisms involved.Methods:Network pharmacology was used to predict the key active ingredients and targets of HXTLD.Surgical methods were used to create a rat model of ischemic stroke.The rats were then given a suspension of HXTLD by ig administration.Erectile function was evaluated by Apomorphine(APO)induction.Real-time fluorescence quantitative reverse transcription-polymerase chain reaction(Real-time PCR)and Western blotting were used to detect the expression of related m RNAs and proteins in rat penile corpus cavernous tissue and brain tissue.Hematoxylin&Eosin(HE)staining was used to investigate structural changes in the penile cavernous tissue.Results:Network pharmacology showed that tumor necrosis factor(TNF),nitric oxide synthase 3(eNOS),and vascular endothelial growth factor(VEGF)were the key targets of HXTLD in the treatment of erectile dysfunction caused by ischemic stroke.Experimental studies showed that HXTLD improved erectile dysfunction caused by ischemic stroke.HE results showed that HXTLD improved the structure of the corpus cavernosa.HXTLD also inhibited the expression of TNF and VEGF proteins in penile tissue(P<0.05)and enhanced the expression of e NOS protein in penile tissue(P<0.05).Conclusion:HXTLD improved the erectile function of rats with erectile dysfunction caused by ischemic stroke by regulating the m RNA and protein levels of TNF,e NOS and VEGF.展开更多
目的探讨活血通络汤外洗联合冲击波治疗跟痛症的临床效果。方法选取2020年1月—2022年12月武警第一机动总队医院收治的80例跟痛症患者为研究对象。按照治疗方法不同分为两组,各40例。对照组采用局部冲击波治疗,观察组在对照组治疗的基...目的探讨活血通络汤外洗联合冲击波治疗跟痛症的临床效果。方法选取2020年1月—2022年12月武警第一机动总队医院收治的80例跟痛症患者为研究对象。按照治疗方法不同分为两组,各40例。对照组采用局部冲击波治疗,观察组在对照组治疗的基础上结合活血通络汤外洗治疗。比较两组治疗后每日跟痛持续时间、视觉模拟评分法(Visual Analog Scale,VAS)评分、Maryland足跟功能评分,统计两组治疗效果、并发症发生率,并随访1年,比较复发率。结果治疗后,观察组每日跟痛持续时间为(0.6±0.2)h,短于对照组的(5.6±1.8)h,差异有统计学意义(t=17.329,P<0.05)。治疗后,观察组VAS评分低于对照组,差异有统计学意义(P<0.05)。治疗后,两组Maryland足跟功能评分均高于治疗前,且观察组高于对照组,差异有统计学意义(P均<0.05)。观察组治疗总有效率高于对照组,1年复发率低于对照组,差异有统计学意义(P均<0.05)。两组并发症发生率比较,差异无统计学意义(P>0.05)。结论跟痛症患者在使用冲击波治疗基础上联合应用活血通络汤外洗,可快速缓解患者足跟部疼痛,改善足部功能,提高治疗有效率,降低复发率,且不良反应少,安全性高。展开更多
基金supported by“3+3”Project for the Inheritance of Beijing Traditional Chinese Medicine(No.2016-SZ-C-60)。
文摘Objective:To study the therapeutic effect of Huoxue Tongluo Decoction(HXTLD)on erectile dysfunction caused by ischemic stroke and identify the mechanisms involved.Methods:Network pharmacology was used to predict the key active ingredients and targets of HXTLD.Surgical methods were used to create a rat model of ischemic stroke.The rats were then given a suspension of HXTLD by ig administration.Erectile function was evaluated by Apomorphine(APO)induction.Real-time fluorescence quantitative reverse transcription-polymerase chain reaction(Real-time PCR)and Western blotting were used to detect the expression of related m RNAs and proteins in rat penile corpus cavernous tissue and brain tissue.Hematoxylin&Eosin(HE)staining was used to investigate structural changes in the penile cavernous tissue.Results:Network pharmacology showed that tumor necrosis factor(TNF),nitric oxide synthase 3(eNOS),and vascular endothelial growth factor(VEGF)were the key targets of HXTLD in the treatment of erectile dysfunction caused by ischemic stroke.Experimental studies showed that HXTLD improved erectile dysfunction caused by ischemic stroke.HE results showed that HXTLD improved the structure of the corpus cavernosa.HXTLD also inhibited the expression of TNF and VEGF proteins in penile tissue(P<0.05)and enhanced the expression of e NOS protein in penile tissue(P<0.05).Conclusion:HXTLD improved the erectile function of rats with erectile dysfunction caused by ischemic stroke by regulating the m RNA and protein levels of TNF,e NOS and VEGF.
文摘目的探讨活血通络汤在急性缺血性脑卒中(acute ischemic stroke,AIS)患者治疗中的效果及对其对中医证候、神经功能的影响。方法选取82例六安市中医院2021年1月—2022年1月收治的AIS患者作为研究对象,将其分为观察组(41例)和对照组(41例)(随机数字表法)。对照组接受常规西医治疗,观察组在对照组基础上接受活血通络汤治疗。两组治疗时间均为1个月。对两组治疗前后的美国国立卫生研究院卒中量表(National Institute of Health stroke scale,NIHSS)、蒙特利尔认知评估(Montreal Cognitive Assessment,MoCA)评分、中医证候积分、生活质量评分、全血高切黏度、全血低切黏度、血浆纤维蛋白原(fibrinogen,FIB)及D-二聚体(D-dimer,D-D)水平、血管内皮功能及治疗后的临床疗效进行比较。结果与观察组比较,对照组治疗后临床总有效率较低;治疗后两组中医证候各项评分、NIHSS评分、全血高切黏度、全血低切黏度、血浆FIB、D-D水平、血清内皮素-1(endothelin-1,ET-1)、血管性假血友病因子(von Willebrand factor,vWF)水平与治疗前比均降低,其中观察组低于对照组;治疗后两组生活质量评分、MoCA评分、血清一氧化氮(nitric oxide,NO)、血管舒张功能(flow-mediated dilation,FMD)水平与治疗前比均升高,组间进行比较,对照组较低(P<0.05)。结论活血通络汤应用于AIS患者可有效改善中医证候,利于神经功能和认知功能恢复,提高生活质量,调节血液状态和血管内皮功能,疗效显著。
文摘目的探讨活血通络汤外洗联合冲击波治疗跟痛症的临床效果。方法选取2020年1月—2022年12月武警第一机动总队医院收治的80例跟痛症患者为研究对象。按照治疗方法不同分为两组,各40例。对照组采用局部冲击波治疗,观察组在对照组治疗的基础上结合活血通络汤外洗治疗。比较两组治疗后每日跟痛持续时间、视觉模拟评分法(Visual Analog Scale,VAS)评分、Maryland足跟功能评分,统计两组治疗效果、并发症发生率,并随访1年,比较复发率。结果治疗后,观察组每日跟痛持续时间为(0.6±0.2)h,短于对照组的(5.6±1.8)h,差异有统计学意义(t=17.329,P<0.05)。治疗后,观察组VAS评分低于对照组,差异有统计学意义(P<0.05)。治疗后,两组Maryland足跟功能评分均高于治疗前,且观察组高于对照组,差异有统计学意义(P均<0.05)。观察组治疗总有效率高于对照组,1年复发率低于对照组,差异有统计学意义(P均<0.05)。两组并发症发生率比较,差异无统计学意义(P>0.05)。结论跟痛症患者在使用冲击波治疗基础上联合应用活血通络汤外洗,可快速缓解患者足跟部疼痛,改善足部功能,提高治疗有效率,降低复发率,且不良反应少,安全性高。
