Protective Effects of Self-made Compound Taoren Danshen Decoction on Rats with Acute Liver Injury

[Objectives] To study the protective effects of self-made Compound Taoren Danshen Decoction( CTDD) on acute liver injury induced by D-galactosamine in rats,and to explore its mechanism. [Methods]An acute liver injury model was established by intraperitoneal injection of 500 mg/kg of D-galactosamine. The ALT,AST,MDA,SOD and GSH-Px in serum,as well as serum TNF-α,IL-1β and IL-6 levels were measured,and liver tissue lesions were observed under microscope. [Results] CTDD can significantly reduce ALT,AST and MDA levels,increase SOD,GSH-Px activity,and significantly reduce serum TNF-α,IL-1β and IL-6 levels,and improve liver tissue lesions.[Conclusions]CTDD has a protective effect on D-galactosamine-induced acute liver injury in rats,and its mechanism may be related to inhibition of oxidative stress and inflammatory reaction.

Observation on Curative Effect of Self-made Wubeizi Decoction Combined with rhaFGF in Promoting Postoperative Wound Healing in Patients with Anal Fistula

[Objectives]To explore the curative effect of Self-made Wubeizi Decoction combined with recombinant human acidic fibroblast growth factor(rhaFGF)in promoting postoperative wound healing in patients with anal fistula.[Methods]A total of 82 patients with anal fistula who underwent anal fistula resection+use of setons in Luodian Hospital during January and March of 2020 were randomly divided into observation group and control group with 41 cases in each group.The control group was given rhaFGF external application regimen,and the observation group was treated with Self-made Wubeizi Decoction on the basis of the control group.After 3 weeks of treatment,the differences in curative effects of TCM syndromes were compared between the two groups.Besides,the changes in TCM symptom scores,inflammatory mediators[interleukin-12(IL-12),tumor necrosis factor-α(TNF-α),interferon-γ(IFN-γ)],anorectal functions[anal resting pressure(ARP),maximal systolic pressure(MSP)and maximum tolerated volume(MTV)of the anal canal],quality of life[GQOLI-74 scores]were compared.[Results]After 3 weeks of treatment,the total effective rate of the observation group was significantly higher than that of the control group(P<0.05);the levels of TCM symptom scores,IL-12,TNF-α,IFN-γ,ARP,MSP,and MTV in both groups were significantly lower than those before treatment,and these levels in the observation group was significantly lower than that in the control group at the same time(P<0.05).The GQOLI-74 scores of both groups were significantly higher than those before treatment,and the observation group was significantly higher than the control group(P<0.05).[Conclusions]The Self-made Wubeizi Decoction combined with rhaFGF therapy has a significant effect in promoting postoperative wound healing in patients with anal fistula.It can effectively inhibit the local inflammation of patients,facilitate the recovery of anorectal function and improve the quality of life,thus has high clinical application value.

石萆汤治疗肾虚夹湿型弱精子症49例临床观察

目的观察石萆汤治疗肾虚夹湿型弱精子症的疗效。方法将145例符合诊断标准的门诊患者随机分为中药治疗组(A组)49例、西药治疗组(B组)46例、中西医结合治疗组(C组)50例,分别给予石萆汤、左卡尼汀口服液、石萆汤合左卡尼汀口服液治疗,观察3组治疗前与治疗8周后的中医症状评分、精子运动分析活力参数、治疗前后精浆a-葡糖苷酶水平及前列腺液的变化。结果总有效率A组73.46%,B组56.52%,C组88.00%,治疗后在改善中医症状、降低前列腺液白细胞水平上,A组、C组优于B组(P<0.05)。结论石萆汤治疗肾虚夹湿型弱精子症疗效较好。

桂枝芍药知母汤加减治疗风寒湿痹型类风湿关节炎的临床疗效研究

目的:研究风寒湿痹型类风湿性关节炎(Rheumatoid Arthritis,RA)应用桂枝芍药知母汤加减治疗的临床效果。方法:选择我院2015年7月-2018年8月治疗的风寒湿痹型类RA患者60例,随机分为两组,各30例。对照组采用免疫抑制剂治疗,在此基础上,观察组联合桂枝芍药知母汤加减治疗,比较两组患者治疗前后血沉(Erythrocyte Sedimentation Rate,ESR)、类风湿因子(Rheumatoid Factor,RF)、C-反应蛋白(C-reactive Protein,CRP)、免疫球蛋白(IgG)水平、中医证候积分和类风湿关节炎病情活动度评价表(DAS-28)评分情况。结果:治疗前,两组的ESR、RF、CRP、IgG水平、各项中医证候积分(关节疼痛度、屈伸不利、关节怕冷、关节发热)、DAS-28评分比较差异无统计学意义(P>0.05);治疗后,两组IgG水平比较差异无统计学意义(P>0.05),相较之对照组,观察组ESR、RF、CRP、各项中医证候积分、DAS-28评分均明显更低(P<0.05)。结论:采应用桂枝芍药知母汤加减治疗RA,能够有效改善临床症状及相关临床指标,可改善患者预后。

石萆汤治疗肾虚夹湿型少弱精子症不育的疗效及对精液参数、性激素的影响

目的:探讨石萆汤治疗肾虚夹湿型少弱精子症不育的临床效果以及对精液参数、性激素的影响。方法:选取93例肾虚夹湿型少弱精子症不育患者,随机分为研究组48例与对照组45例。对照组给予西医常规治疗,研究组在常规治疗基础上加用石萆汤。对比治疗前后中医症状积分、精液质量[精液量、精子密度、精子活动率、精子活力(a级、a+b级)]以及临床疗效,比较治疗前后性激素水平指标睾酮(T)、黄体生成素(LH)、卵泡刺激素(FSH)。结果:两组治疗后中医症状积分均较治疗前降低,研究组治疗后低于对照组,差异均具有统计学意义(P<0.05);两组治疗后精液量、精子密度、精子活动率、a级精子活力及(a+b)级精子活力均较治疗前增高,研究组治疗后均高于对照组,差异均具有统计学意义(P<0.05);研究组总有效率高于对照组,差异有统计学意义(P<0.05);两组治疗后血清T均较治疗前升高,LH、FSH水平均较治疗前降低,差异均具有统计学意义(P<0.05);研究组治疗后血清T高于对照组,LH、FSH水平低于对照组,差异均具有统计学意义(P<0.05)。结论:石萆汤治疗肾虚夹湿型少弱精子症不育患者,可显著减轻患者中医症状,提高精液质量,改善性激素水平,提升临床疗效。

自拟湿痹汤结合针灸推拿治疗膝关节骨性关节炎的临床效果研究

目的:探讨自拟湿痹汤结合针灸推拿治疗膝关节骨性关节炎的临床效果。方法:2018年6月-2019年9月收治膝关节骨性关节炎患者90例,按不同治疗方法分为两组。观察组采用自拟湿痹汤联合针灸推拿治疗;对照组采用针灸推拿治疗。比较两组临床疗效及骨关节炎指数(WOMAC)评分。结果:治疗4周后,观察组治疗总有效率高于对照组,WOMAC评分低于对照组,差异均有统计学意义(P<0.05)。结论:自拟湿痹汤结合针灸推拿可提高膝关节骨性关节炎的治疗效果,降低WOMAC评分,改善膝关节功能,有利于患者恢复。

石萆汤对环磷酰胺干预后大鼠精子DNA碎片率及性激素的影响

目的研究石萆汤对环磷酰胺干预后大鼠精子的DNA碎片率及性激素的影响。方法随机选取8只大鼠为正常组,其余32只大鼠为造模组,造模组连续14 d腹腔注射环磷酰胺(20 mg·kg^(-1)·d^(-1))制备大鼠少弱精子症模型,然后将造模组随机分为模型组、石萆汤低、中、高剂量(6.5625 g/kg、13.125 g/kg、26.25 g/kg)组,每组8只。正常组和模型组给予生理盐水灌胃,石萆汤低、中、高剂量组分别给予相对应的中药剂量灌胃,中药干预35 d后,采用流式细胞仪检测大鼠精子DNA碎片率(DFI);酶联免疫吸附测定法(ELISA法)检测血清中卵泡刺激素(FSH)、黄体生成素(LH)、睾酮(T)、泌乳素(PRL)、雌激素(E_(2))的水平。结果与正常组比,模型组大鼠精子DFI显著增高(P<0.01);与模型组相比,治疗后石萆汤低、中、高剂量组的大鼠精子DFI显著下降(P<0.01);血清性激素水平检测结果显示,模型组T、FSH和LH含量显著降低(P<0.01);E2和PRL含量显著升高(P<0.01);石萆汤低、中、高剂量组均能改善大鼠血清性激素水平,以中剂量最为明显,尤其是提高T的水平(P<0.01)。结论石萆汤能平衡环磷酰胺干预后大鼠的性激素水平,降低大鼠的精子DFI,为临床研究提供实验依据和基础。

石萆汤通过调节线粒体膜蛋白PHB保护线粒体膜电位增强精子功能的探究

目的通过对90例男性不育弱精子症患者及30例正常男性进行分组处理观察石萆汤治疗弱精子症的可能靶点,探究石萆汤治疗肾虚夹湿型弱精子症的分子生物学机制。方法选择2016年8月~2018年6月在福建中医药大学附属人民医院男科门诊就诊的男性不育弱精子症患者和生殖体检者共120例,其中30例正常组与30例弱精子症组患者暂时未接受治疗,60例治疗组患者给予石萆汤治疗16周。治疗前后采用Western blot检测患者精子线粒体膜蛋白PHB及JC-1流式细胞仪检测线粒体膜电位。结果由Western blot结果,我们可以看到弱精子症组的患者精子线粒体膜蛋白PHB表达量明显低于正常组(P<0.05);在给予石萆汤治疗16周后,治疗组患者精子线粒体膜蛋白PHB蛋白表达量显著高于弱精子症组(P<0.05)。通过对3组患者精子JC-1染色后进行流式细胞仪检测发现弱精子症组患者精子线粒体膜电位(红色荧光散点)与正常组和治疗组相比偏向下移,提示弱精子组患者精子症线粒体膜电位降低。而经过石萆汤治疗16周后流式红色荧光散点上移差异具有统计学意义(P<0.05)。结论石萆汤治疗肾虚夹湿型弱精子症可能是通过调控精子线粒体膜蛋白PHB表达进而改变精子线粒体膜电位,最终提高精子活力,增强精子功能,值得在今后临床中应用,同时线粒体膜蛋白PHB可对精子功能进行衡量,可在今后的临床检验中进行检测反映精子功能与状态。