A novel approach is proposed for direct quantitative analysis of thiabendazole in the orange extract by using excitation-emission matrix fluorescence coupled with second-order calibration methods based on the alternat...A novel approach is proposed for direct quantitative analysis of thiabendazole in the orange extract by using excitation-emission matrix fluorescence coupled with second-order calibration methods based on the alternating trilinear decomposition(ATLD) and the alternating normalization-weighted error(ANWE) algorithms,respectively. The average recoveries of thiabendazole in the orange extract by using ATLD and ANWE with an estimated component number of two were 99.7 ± 3.3% and 103.5 ± 4.1%,respectively. Furthermore,the accuracy of the two algorithms was also evaluated through elliptical joint confidence region(EJCR) tests as well as figures of merit,such as sensitivity(SEN),selectivity(SEL) and limit of detection(LOD). The experimental results demonstrate that both algorithms have been satisfactorily applied to the determination of thiabendazole in orange extract,and the perform-ance of ANWE is slightly better than that of ATLD.展开更多
High performance liquid chromatography(HPLC) is one of the analytical techniques used most frequently. Its chief limitation lies in the need to avoid the overlap of chromatographic peaks as much as possible. In this p...High performance liquid chromatography(HPLC) is one of the analytical techniques used most frequently. Its chief limitation lies in the need to avoid the overlap of chromatographic peaks as much as possible. In this paper, the overlapped chromatograms and spectra system of 1-chloro-2,4-dinitrobenzene and 3,5-dinitrobenzoic acid in the presence of 2,4-dinitrobenzoic acid were resolved by using the alternating trilinear decomposition(ATLD) combined with HPLC-DAD. Moreover, both were determined simultaneously, whose recoveries were(102.1±2.2)% and(97.6±3.6)%, respectively. The satisfactory results show that this experiment can be easily performed without paying out time-consuming and complicated procedures and quantifying accurately. It revealed the ATLD algorithm was one of the approaches to solve second-order calibration problem in HPLC-DAD.展开更多
基金Supported by the National Natural Science Foundation of China (Grant Nos. 20775025 and 20435010)973 Advanced Research Project (Grant No. 2007CB- 216404)
文摘A novel approach is proposed for direct quantitative analysis of thiabendazole in the orange extract by using excitation-emission matrix fluorescence coupled with second-order calibration methods based on the alternating trilinear decomposition(ATLD) and the alternating normalization-weighted error(ANWE) algorithms,respectively. The average recoveries of thiabendazole in the orange extract by using ATLD and ANWE with an estimated component number of two were 99.7 ± 3.3% and 103.5 ± 4.1%,respectively. Furthermore,the accuracy of the two algorithms was also evaluated through elliptical joint confidence region(EJCR) tests as well as figures of merit,such as sensitivity(SEN),selectivity(SEL) and limit of detection(LOD). The experimental results demonstrate that both algorithms have been satisfactorily applied to the determination of thiabendazole in orange extract,and the perform-ance of ANWE is slightly better than that of ATLD.
文摘High performance liquid chromatography(HPLC) is one of the analytical techniques used most frequently. Its chief limitation lies in the need to avoid the overlap of chromatographic peaks as much as possible. In this paper, the overlapped chromatograms and spectra system of 1-chloro-2,4-dinitrobenzene and 3,5-dinitrobenzoic acid in the presence of 2,4-dinitrobenzoic acid were resolved by using the alternating trilinear decomposition(ATLD) combined with HPLC-DAD. Moreover, both were determined simultaneously, whose recoveries were(102.1±2.2)% and(97.6±3.6)%, respectively. The satisfactory results show that this experiment can be easily performed without paying out time-consuming and complicated procedures and quantifying accurately. It revealed the ATLD algorithm was one of the approaches to solve second-order calibration problem in HPLC-DAD.
