The aim of this study is to reveal the regulation mechanism of the effect of Semen vaccariae and Taraxacu mogono on the cell-cell adhersion molecule, E-cadherin and β-catenin on the proliferation role and secretion f...The aim of this study is to reveal the regulation mechanism of the effect of Semen vaccariae and Taraxacu mogono on the cell-cell adhersion molecule, E-cadherin and β-catenin on the proliferation role and secretion function of bovine mammary epithelial cells cultured in vitro. Firstly, the epithelial character of bovine mammary epithelial cells was authenticated using immunofluorescence, then the cell grow curve was observed and investigated after S. vaccariae and T. mogono treatment. On the effect of S. vaccariae and T. mogono, cell adhesion molecules E-cadherin, β-catenin and CycinD1 mRNA and protein were detected by qRT-PCR and Western blotting, respectively. The results showed that the cellular keratin 18 expressed positively and proliferfated vigorously after S. vaccariae and T. mogono treament. The mRNA and protein levels of E-cadherin and CycinD1 were remarkably higher （P〈0.05） in 36 h after S. vaccariae and T. mogono treatment. The cell proliferation at 36 h was increased significantly （P〈0.05）. In conclusion, S. vaccariae and T. mogono have a positive impact on the cell proliferation and an effect on the adhesion molecules E-cadherin, β-catenin and CycinD1 in the Wnt signaling pathway.展开更多
Objective To compare the differences of effect on infantile amblyopia treated with auricular point sticking therapy and regular comprehensive therapy of western medicine.Methods Two hundreds and thirty cases of amblyo...Objective To compare the differences of effect on infantile amblyopia treated with auricular point sticking therapy and regular comprehensive therapy of western medicine.Methods Two hundreds and thirty cases of amblyopia patients were randomly divided into two groups,namely the group of auricular point sticking therapy,which had 120 cases（212 eyes）,and the group of comprehensive therapy,which had 110 cases（194 eyes）.The seed of Semen Vaccariae was applied at the ear point such as the eye,ear Shenmen,the liver,the spleen and the kidney.Regular comprehensive therapy of western medicine included covering method,vision corrected with glasses and fine exercise.Vision improvements of the two groups were observed with a three-year follow up.Results The effective rates of the auricular point sticking therapy group and comprehensive therapy group of ametropic amblyopia were 81.0%（64/79） and 52.2%（36/69）,respectively.The effective rates of anisometropic amblyopia of the two groups were 73.1%（49/67） and 47.7%（31/65）,respectively.And the effective rates of the two groups of strabismic amblyopia were 71.2%（47/66） and 45.0%（27/60）,respectively.The therapeutic effects of auricular point sticking therapy on all kinds of amblyopia were better than those of the comprehensive therapy（all P0.05）.Conclusion The effect of auricular point sticking therapy on infantile amblyopia is better than that of regular comprehensive therapy of western medicine,which can obviously improve the vision of the patients with easy manipulations.展开更多
基金supported by the National Basic Research Program of China(2011CB100804)
文摘The aim of this study is to reveal the regulation mechanism of the effect of Semen vaccariae and Taraxacu mogono on the cell-cell adhersion molecule, E-cadherin and β-catenin on the proliferation role and secretion function of bovine mammary epithelial cells cultured in vitro. Firstly, the epithelial character of bovine mammary epithelial cells was authenticated using immunofluorescence, then the cell grow curve was observed and investigated after S. vaccariae and T. mogono treatment. On the effect of S. vaccariae and T. mogono, cell adhesion molecules E-cadherin, β-catenin and CycinD1 mRNA and protein were detected by qRT-PCR and Western blotting, respectively. The results showed that the cellular keratin 18 expressed positively and proliferfated vigorously after S. vaccariae and T. mogono treament. The mRNA and protein levels of E-cadherin and CycinD1 were remarkably higher （P〈0.05） in 36 h after S. vaccariae and T. mogono treatment. The cell proliferation at 36 h was increased significantly （P〈0.05）. In conclusion, S. vaccariae and T. mogono have a positive impact on the cell proliferation and an effect on the adhesion molecules E-cadherin, β-catenin and CycinD1 in the Wnt signaling pathway.
文摘Objective To compare the differences of effect on infantile amblyopia treated with auricular point sticking therapy and regular comprehensive therapy of western medicine.Methods Two hundreds and thirty cases of amblyopia patients were randomly divided into two groups,namely the group of auricular point sticking therapy,which had 120 cases（212 eyes）,and the group of comprehensive therapy,which had 110 cases（194 eyes）.The seed of Semen Vaccariae was applied at the ear point such as the eye,ear Shenmen,the liver,the spleen and the kidney.Regular comprehensive therapy of western medicine included covering method,vision corrected with glasses and fine exercise.Vision improvements of the two groups were observed with a three-year follow up.Results The effective rates of the auricular point sticking therapy group and comprehensive therapy group of ametropic amblyopia were 81.0%（64/79） and 52.2%（36/69）,respectively.The effective rates of anisometropic amblyopia of the two groups were 73.1%（49/67） and 47.7%（31/65）,respectively.And the effective rates of the two groups of strabismic amblyopia were 71.2%（47/66） and 45.0%（27/60）,respectively.The therapeutic effects of auricular point sticking therapy on all kinds of amblyopia were better than those of the comprehensive therapy（all P0.05）.Conclusion The effect of auricular point sticking therapy on infantile amblyopia is better than that of regular comprehensive therapy of western medicine,which can obviously improve the vision of the patients with easy manipulations.
