Corrigendum: Activation of autophagy by Citri Reticulatae Semen extract ameliorates amyloid-beta-induced cell death and cognition deficits in Alzheimer’s disease

In the article titled“Activation of autophagy by Citri Reticulatae Semen extract ameliorates amyloid-beta-induced cell death and cognition deficits in Alzheimer’s disease”published on pages 2467-2479,Issue 11,Volume 19 of Neural Regeneration Research(Tang et al.,2024),there are some errors in selecting the appropriate images in Figure 7 by authors during assembling the images.

Effects of flavonoids from Semen Cuscutae on the reproductive system in male rats

Aim: To evaluate the effects of the flavonoids extracted from the Semen Cuscutae (FSC) on the reproductive and en-docrine functions in male rats. Methods: (1) FSC were obtained from the semen of Cuscuta sinensis Lam throughsolvent extraction and polyamide columnar chromatography; (2) Effect of FSC on the reproductive organs was assessedin immature rats. Rats were administered FSC through gastric gavage at a dose of 300 mg/kg per day for 7 days and theweights of testis, epididymis, seminal vesicle and pituitary gland were then observed; (3) To observe the effect of FSCon the reproductive endocrine function: same dose level of FSC was given to male rats of different age groups for 7days; on day 8, the plasma testosterone (T), estradiol (E_2) and LH were determined by RIA, the specific binding ofLH was estimated and the testes were weighed. (4) Effect of FSC on LH secretion was assessed in vitro on culturedadenohypophysis. (5) Effect of FSC on T secretion was assessed in vitro on Leydig cell culture. Results: FSC in-creased the weights of testis, epididymis and pituitary gland, and stimulated T and LH secretion both in vitro and inimmature rats. Conclusion: FSC invigorates the reproductive system and reproductive endocrine function in male rats.

Identification and determination of (+)-sesamin in Semen Cuscutae by capillary GC and GC-MS

(+)-Sesamin was found in Semen Cuscutae for the first time. A rapid and simple approach for the analysis of(+)-sesamin in different sources of Semen Cuscutae is proposed, which used GC-FID for the determination of(+)-sesamin and CC-MS for its identification. The result suggested that this approach could be used to identify Semen Cuscutae from various sources based on the different content of(+)-sesamin in them.

Activation of autophagy by Citri Reticulatae Semen extract ameliorates amyloid-beta-induced cell death and cognition deficits in Alzheimer’s disease

Amyloid-beta-induced neuronal cell death contributes to cognitive decline in Alzheimer’s disease.Citri Reticulatae Semen has diverse beneficial effects on neurodegenerative diseases,including Parkinson’s and Huntington’s diseases,however,the effect of Citri Reticulatae Semen on Alzheimer’s disease remains unelucidated.In the current study,the anti-apoptotic and autophagic roles of Citri Reticulatae Semen extract on amyloid-beta-induced apoptosis in PC12 cells were first investigated.Citri Reticulatae Semen extract protected PC12 cells from amyloid-beta-induced apoptosis by attenuating the Bax/Bcl-2 ratio via activation of autophagy.In addition,Citri Reticulatae Semen extract was confirmed to bind amyloid-beta as revealed by biolayer interferometry in vitro,and suppress amyloid-beta-induced pathology such as paralysis,in a transgenic Caenorhabditis elegans in vivo model.Moreover,genetically defective Caenorhabditis elegans further confirmed that the neuroprotective effect of Citri Reticulatae Semen extract was autophagy-dependent.Most importantly,Citri Reticulatae Semen extract was confirmed to improve cognitive impairment,neuronal injury and amyloid-beta burden in 3×Tg Alzheimer’s disease mice.As revealed by both in vitro and in vivo models,these results suggest that Citri Reticulatae Semen extract is a potential natural therapeutic agent for Alzheimer’s disease via its neuroprotective autophagic effects.

Research Progress on Chemical Constituents and Pharmacological Effect of Semen Cuscutae

In this paper,by summarizing the literature in recent years,it is considered that the chemical constituents of S.cuscutae are mainly flavonoids.The main pharmacological effects include improving osteoporosis,improving ovarian function,improving drug-induced reproductive toxicity,regulating spermatogenic cell apoptosis,resisting liver fibrosis,resisting inflammation,improving cardiovascular function,protecting fetus,protecting synapses and so on.By summarizing the chemical constituents and pharmacological effects of S.cuscutae,this study aims to better study the mechanism of anti-osteoporosis based on the fact that S.cuscutae has the function of tonifying liver and kidney.

A Textual Research on Materia Medica of Origin of Orthonym and Synonym of Cuscutae Semen

A textual research on materia medica of origin of orthonym and synonym of Cuscutae Semen and synonym of Nu Luo was conducted in this study,and the trade name,regional synonym and nickname of Cuscutae Semen were reviewed.The records of orthonym and synonym of Cuscutae Semen in the past dynasties,and the first published literature and writing date of orthodox and synonym were summarized.The textual research showed that Nu Luo and Cuscutae Semen were not the same plant,which had positive significance for the identification of the origin and modern application of Cuscutae Semen.

Exploring the relationship between ambient sulfur dioxide and semen quality parameters:A systematic review and meta-analysis

Objective:To investigate the relationship between ambient sulfur dioxide(SO2)exposure and semen quality parameters.Methods:A systematic literature search was conducted to identify relevant studies investigating the association between SO2 exposure and semen quality parameters.This search encompassed the timeframe from January 2000 to May 2023 and included electronic databases such as Web of Science,Google Scholar,PubMed,Cochrane,and Scopus.Pooled effect estimates with 95%confidence intervals(CI)were calculated using percent changes(PC).The meta-analysis included seven studies with 6711 participants and 15087 semen samples.Results:The results revealed a significant negative association between ambient SO2 exposure and certain semen quality parameters.In particular,SO2 exposure was associated with a significant decrease in progressive motility(PC=0.032;95%CI:-0.063 to-0.001;P=0.044)and sperm concentration(PC=-0.020;95%CI:-0.036 to-0.005;P=0.012).However,no statistically significant associations were observed for total sperm count(PC=-0.038;95%CI:-0.079 to 0.003;P=0.070),seminal fluid volume(PC=-0.009;95%CI:-0.048 to-0.030;P=0.662)and sperm motility(PC=-0.17;95%CI:-0.363 to 0.022;P=0.830).In addition,the results of the subgroup analysis revealed specific variables that were associated with the decrease in relevant sperm parameters.Conclusions:This systematic review and meta-analysis provides compelling evidence supporting a consistent negative association between exposure to ambient SO2 and semen quality parameters.

Effect of Moringa oleifera on semen quality profiles in endangered Teressa goat under tropical humid island ecosystem

Objective:To investigate the effect of Moringa oleifera leaf extract on semen quality parameters in Teressa goat buck.Methods:A total of 25 semen samples from six bucks were selected for the study.Spermatozoa of 150×10^(6) were incubated in 0,300,500 and 700μg of leaf extract as groupⅠ,Ⅱ,Ⅲ and Ⅳ,respectively.Liquid stored semen samples were analysed for motility,viability,total sperm abnormalities,plasma membrane,acrosomal and nuclear integrities,seminal plasma intracellular enzymes[aspartate aminotransferase(AST),alanine aminotransferase(ALT)and lactate dehydrogenase(LDH)]and total antioxidant capacity(TAC)and spermatozoa malondialdehyde(MDA)and cholesterol efflux in comparison with the control group(groupⅠ)for up to 72 h.Results:Moringa oleifera leaf extract(500μg/150×10^(6) spermatozoa)-treated semen had significantly higher motility,viability,plasma membrane,acrosomal and nuclear integrities,and TAC(P<0.05),and had significantly lower total sperm abnormalities,AST,ALT,LDH,MDA production and cholesterol efflux compared to those in other Moringa oleifera leaf extract treated(300 or 700μg/150×10^(6) spermatozoa)and control groups at different hours of liquid storage(P<0.05).Semen quality parameters and TAC showed an increasing trend and total sperm abnormalities,MDA production,leakage of intra-cellular enzymes and cholesterol efflux showed a decreasing trend from group Ⅰto group Ⅲand then an opposite trend from group Ⅲ to group Ⅳ at different hours of liquid storage.Conclusions:Moringa oleifera leaf extract 500μg/150×10^(6) spermatozoa can be used for liquid semen preservation in Teressa goat at Andaman and Nicobar Islands.

Adding chitosan nanoparticles of green tea extract in diluent and thawing temperatures ameliorate the post-thawed quality of Boer buck semen

Objective:To improve the quality of post-thawing Boer buck semen for artificial insemination by adding green tea extract chitosan nanoparticles to skimmed egg yolk diluent,and the proper thawing temperature.Methods:The ejaculate of Boer buck was added to skimmed egg yolk diluent without(the control group)and with adding 1μg of chitosan nanoparticles of green tea extract per mL of diluent(the treatment group).Then,the diluted semen was filled in French mini straws containing 60×106 live sperm per straw,frozen in a standard protocol,and stored as frozen semen at−196℃for a week.Six replicates from each group were diluted for 30 s at 37℃or 39℃sterile water to evaluate the semen quality.Results:Post-thawing(at 37℃or 39℃)of live sperm,progressive motility,and plasma membrane integrity were lower compared to those of the pre-freezing stage(P<0.05).Thawing at 37℃resulted in no significant difference in live sperm,progressive motility,and plasma membrane between the control group and the treatment group(P>0.05).The live sperm,progressive motility,and plasma membrane of the treatment group in the pre-freezing stage,and post-thawed at 39℃were higher compared to those of the control group(P<0.05).There was no significant difference in malondialdehyde(MDA)concentration,DNA fragmentation,and catalase concentration of thawing at 37℃compared to those of 39℃in the same group.The MDA concentration and DNA fragmentation in thawing at 37℃and 39℃of the treatment group were significantly lower than those of the control group(P<0.05).However,the catalase concentration in thawing at 37℃and 39℃of the treatment group was not significantly different than the control group(P>0.05).Conclusions:Higher quality post-thawing Boer buck semen is achieved by adding 1μg/mL of chitosan nanoparticles of green tea extract to the skimmed egg yolk diluent and thawing at 39℃.

Patterns of alteration in boar semen quality from 9 to 37 months old and improvement by protocatechuic acid

Background Comprehending the patterns of alteration in boar semen quality and identifying effective nutritional interventions are crucial for enhancing the productivity of commercial pig systems.This study aimed to examine the alteration in semen quality in boars,and assess the impact of protocatechuic acid(PCA)on semen quality during the phase of declining semen quality.Methods In Exp.1,a total of 38 Pig Improvement Company(PIC)boars were selected and their semen quality data were recorded from the age of 9 to 37 months.In Exp.2,18 PIC boars(28 months old)were randomly assigned into three groups(n=6)and fed a basal diet,a basal diet containing 500 or 1,000 mg/kg PCA,respectively.The experiment lasted for 12 weeks.Results The semen volume,concentration,and total number of spermatozoa in boars exhibited an increase from 9 to 19 months old and showed a significant linear decreased trend in 28,24,and 22 months old.Sperm motility displayed an upward trajectory,reaching its peak at 20 months of age,and showed a significant linear decreased trend at 20 months old.Dietary supplementation of PCA demonstrated an effect to mitigate the decrease in semen volume,concentration of spermatozoa,total number of spermatozoa(P>0.05),and significantly increased the sperm motility(P<0.05).Moreover,supplementation of 1,000 mg/kg PCA significantly increased the sperm viability(P<0.05).Analysis on cellular signaling pathways revealed that PCA restored serum testosterone levels and alleviated oxidative damage by upregulating the expression of HO-1,SOD2,and NQO1 in testicular stromal cells.Notably,PCA can enhance phosphorylation by selectively binding to AMP-activated protein kinase(AMPK)protein,thereby improving sperm mitochondrial function and augmenting sperm motility via PGC-1/Nrf1.Conclusions These data elucidated the pattern of semen quality variation in boars within the age range of 9 to 37 months old,and PCA has the potential to be a natural antioxidant to enhance sperm quality through modulation of the AMPK/PGC-1/Nrf1 signaling pathway.

Source and level of dietary iron influence semen quality by affecting inflammation, oxidative stress and iron utilization levels in boars

Background Boars fed a mixed form of inorganic and organic iron in excess of the NRC recommended levels still develop anemia,which suggested that the current level and form of iron supplementation in boar diets may be inappropriate.Therefore,56 healthy Topeka E line boars aged 15–21 months were randomly divided into 5 groups:basal diet supplemented with 96 mg/kg ferrous sulfate(FeSO_(4))and 54 mg/kg glycine chelated iron(Gly-Fe,control);80 mg/kg or 115 mg/kg Gly-Fe;80 mg/kg or 115 mg/kg methionine hydroxyl analogue chelated iron(MHA-Fe,from CalimetFe)for 16 weeks.The effects of dietary iron supplementation with different sources and levels on semen quality in boars were investigated.Results 1)Serum Fe and hemoglobin concentrations were not affected by reduced dietary iron levels in the 80 mg/kg or 115 mg/kg Gly-Fe and MHA-Fe groups compared with the control group(P>0.05).2)Serum interleukin-6(IL-6)and sperm malondialdehyde(MDA)levels in the 80 mg/kg or 115 mg/kg MHA-Fe groups were lower than those in the control group(P<0.05),and higher serum superoxide dismutase levels and lower MDA levels in the 115 mg/kg MHA-Fe group(P<0.05).3)Boars in the 80 mg/kg or 115 mg/kg Gly-Fe and MHA-Fe groups had lower serum hepcidin(P<0.01),ferritin(P<0.05),and transferrin receptor(P<0.01)concentrations,and boars in the 115 mg/kg MHA-Fe group had higher seminal plasma Fe concentrations compared with the control group.4)Boars in the 80 mg/kg and 115 mg/kg MHA-Fe groups had lower abnormal sperm rate and in situ oscillating sperm ratio compared to the control group at weeks 12 and/or 16 of the trial.However,the effect of Gly-Fe on improving semen quality in boars was not evident.5)Serum IL-6 level was positively correlated with hepcidin concentration(P<0.05),which in turn was significantly positively correlated with abnormal sperm rate(P<0.05).Furthermore,significant correlations were also found between indicators of iron status and oxidative stress and semen quality parameters.Conclusions Dietary supplementation with 80 mg/kg or 115 mg/kg MHA-Fe did not induce iron deficiency,but rather reduced serum inflammatory levels and hepcidin concentration,alleviated oxidative stress,increased body iron utilization,and improved semen quality in adult boars.

The Effects of Age and Ejaculatory Abstinence on Semen Quality and Reproductive Hormones in Africa and the Middle East

The aim of this study was to retrospectively evaluate the effects of male age and ejaculatory abstinence on semen parameters and reproductive hormones among men residing in Africa and the Middle East. A total of 70,142 semen analysis results were analysed and grouped according to the age intervals (16 - 20, 21 - 30, 31 - 40, 41 - 50, 51 - 60, >60) and ejaculatory abstinence (<2 days, 2 - 5 days and >5 days). Semen parameters i.e. volume, concentration, progressive motility, total progressively motile count, morphology, total normal sperm count, DNA fragmentation, viability, sORP, normed sORP were specifically evaluated. Additionally, for each age interval, reproductive hormones i.e. estradiol, luteinizing hormone, follicle stimulating hormone, testosterone and prolactin were evaluated. Semen volume, total progressively motile count, sperm morphology and total normal sperm count constantly decrease significantly after the age of 30 years. Sperm concentration started declining significantly after the age of 50 years. There was a constant age- related increase in number of spermatozoa with damaged DNA. sORP constantly increased up to 60 years. Furthermore, constantage-related decreases in FSH, serum testosterone and prolact in were observed from patients aged between 16 years and 60 years. Semen volume, sperm concentration, progressive motility and normal morphology were significantly higher in patients having > 5 days of abstinence. Patients having > 5 days of abstinence had the lowest normed sORP. Male age significantly affects sperm parameters and reproductive hormones in fertile and infertile men residing in Africa and the Middle East. Prolonged abstinence days provides better semen quality.

Study on the Quality of Boar Semen in Kandal Province,Cambodia

In pig production,the selection of parent stock with high capacity and quality is a very important factor in order to obtain high-yield offspring.Therefore,a“study on the quality of boar semen in Kandal province”was conducted for the purpose of comparing and evaluating the quality of boar semen(VAC:Volume Activity Concentration)between Duroc breeds,Yorkshire breeds and Landrace breeds.The study was carried out according to an observation study by selecting three breeds of boars around 3 years old,including two Duroc,two Yorkshire and two Landrace.Each boar was observed in three months,three times a month,and the interval was three to four days.The study took place in Takhmao City,Kandal Province,Cambodia.The results showed that the semen volume per ejaculate per head obtained from Yorkshire boar was an average of 250±76.16 mL,which was higher than the semen volume of Landrace with an average of 173.33±59.73 mL.And the semen volume of Duroc was an average of 149.44±72.70 mL.This value indicates that the semen volume per ejaculate per head was significantly different(p<0.01).The activity or mobility of spermatozoa in the semen between the three breeds differed with slightly significant difference(p<0.05),with the spermatozoa scored from the Yorkshire being 0.76±0.06,Landrace being 0.79±0.05 and Duroc being 0.83±0.03.The semen concentration showed that the Yorkshire had an average spermatozoa concentration of 568.89×10^(6) sperm cells/mL,and the Landrace had an average spermatozoa concentration of 321.11×10^(6) sperm cells/mL and the Duroc an average spermatozoa concentration of 568.86×10^(6) sperm cells/mL,which further confirmed that there was a significant difference in the statistical meaning(p<0.001).The semen quality(VAC)per one ejaculate between the three breeds showed significant difference(p<0.05),in which the VAC of the Yorkshire was 39.31×10^(9) sperms per ejaculate,Landrace was 44.54×10^(9) sperm cells per ejaculate and Duroc was 70.28×10^(9) sperm cells per ejaculate.Based on the above results,we can conclude that the semen quality(VAC)values of the three breeds of pigs are good,which can save the breeding because it shows the excellent quality of each breed.

Study on the regulatory effect of liver X receptor in HEK293 cells by six main diterpene esters in Semen Euphorbiae

Background:To study the effects of the main diterpene esters in Euphorbia factor L_(1),L_(2),L_(3),L_(7a),L_(7b)and L_(8)on the transcriptional activity and protein expression of liver X receptor(LXR).Methods:The effect of the main diterpene ester components in Semen Euphorbiae on the viability of HEK293 cells were studied by MTT assay.The LXR-Luc plasmid vector was transfected into HEK293 cells and treated with Euphorbia factor L_(1),L_(2),L_(3),L_(7a),L_(7b)and L_(8)for 24 h.The effect of the main diterpene ester components of Semen Euphorbiae on LXR-Luc luciferase activity was investigated by dual luciferase reporter gene system,and the expression of LXRαprotein was detected by Western Blot.Results:Euphorbia factor L_(1),L_(2),L_(3),L_(7a),L_(7b)and L_(8)could significantly reduce the relative luciferase activity(RLU)of LXRα,and the expression level of LXRαprotein was significantly down-regulated.Conclusion:Euphorbia factor L_(1),L_(2),L_(3),L_(7a),L_(7b)and L_(8)can inhibit the expression of LXR protein level,which may be achieved by inhibiting the transcriptional activity of LXR.

药用槟榔与嫩槟榔对小鼠的经口急性毒性比较研究

本研究旨在比较嫩槟榔与药用槟榔的毒性,以期为嫩槟榔作为兽用中药原料使用的安全性提供支撑。使用经济合作与发展组织(OECD)的固定剂量法,测定药用槟榔与嫩槟榔的半数致死量(LD_(50))。选择5000 mg/kg作为限度试验测试剂量,每个给药组选取10只雌性小鼠,再选取3只小鼠作为空白对照组,给予等剂量的生理盐水,通过灌胃给药后,观察14 d,通过观测各组小鼠临床表现、血液学参数、体增重、脏器指数、主要器官的组织病理学等来比较药用槟榔以及嫩槟榔对小鼠的急性毒性作用。观察期结束后,药用槟榔组与嫩槟榔组小鼠均未出现异常及死亡。嫩槟榔组小鼠的红细胞分布宽度(RDW-CV、RDW-SD)与空白对照组相比显著降低(P<0.05);药用槟榔组与嫩槟榔组小鼠的体增重、脏器指数与空白对照组相比均无显著性差异(P>0.05);药用槟榔组与嫩槟榔组小鼠的组织病理学检查结果与空白对照组相比均无明显差异。结果提示,药用槟榔和嫩槟榔均为实际无毒(LD50>5000 mg/kg),均具有较高的安全性。

肉豆蔻活性成分去氢二异丁香酚调控MDM2/p53信号通路抗结直肠癌的药效及机制

目的探讨去氢二异丁香酚(DDIE)抑制结直肠癌的作用和机制。方法通过中药系统药理学数据库和分析平台(TCMSP)和SwissTargetPrediction数据库预测DDIE的靶点,利用人类基因(GeneCards)和在线人类孟德尔遗传(OMIM)数据库收集结直肠癌疾病靶点,将药物与疾病交集靶点导入String数据库进行蛋白质-蛋白质相互作用(PPI)分析,运用Cytoscape 3.9.1软件内置插件计算节点拓扑参数值并筛选核心靶点,采用Metascape数据库进行京都基因与基因组百科全书(KEGG)和基因本体(GO)富集分析。并采用体内外实验对富集到的核心靶点和关键通路进行验证。采用细胞计数试剂盒(CCK-8)法检测DDIE对小鼠结直肠癌细胞CT26、人结直肠癌细胞HCT116、人结肠上皮细胞NCM460细胞活力的影响;划痕实验检测DDIE对HCT116细胞迁移能力的影响;Hoechst染色实验、流式细胞术检测DDIE对CT26和HCT116细胞凋亡的影响。Western blot法检测DDIE对CT26、HCT116细胞中促凋亡蛋白B淋巴细胞瘤-2相关X蛋白(Bax)、胱天蛋白酶-3(Caspase-3)的表达。利用SPF级雄性Balb/c小鼠构建CT26结直肠癌皮下移植瘤模型,24只小鼠分为模型组、伊立替康(CPT11)组(60 mg/kg,腹腔注射)、DDIE低剂量(25 mg/kg,灌胃)组、DDIE高剂量组(50 mg/kg,灌胃),记录小鼠体质量、肿瘤大小,苏木精-伊红(HE)染色法观察各组小鼠肿瘤组织病理学变化情况,Western blot法检测DDIE对结直肠癌细胞和肿瘤组织中肿瘤蛋白p53(p53)和双微体同源基因2(MDM2)蛋白表达的影响。结果网络药理学分析结果显示,DDIE抗结直肠癌作用与p53信号通路相关。CCK-8结果显示DDIE可抑制结直肠癌CT26、HCT116细胞活力,其IC50分别为53.56μmol/L、63.36μmol/L,而对正常上皮细胞NCM460的活力影响较小,其IC50>100μmol/L。划痕实验结果显示,10μmol/L、20μmol/L DDIE呈剂量依赖性抑制HCT116细胞迁移能力(P<0.001)。Hoechst染色实验和流式细胞术结果显示,50μmol/L DDIE能明显增加CT26和HCT116细胞凋亡率(P<0.05,P<0.001);Western blot结果显示,25μmol/L和50μmol/L DDIE能明显增加HCT116细胞中促凋亡蛋白Bax、Caspase-3的表达水平(P<0.05,P<0.01,P<0.001),以及CT26细胞中Bax的表达水平(P<0.05,P<0.01)。动物实验结果显示,DDIE能明显抑制小鼠肿瘤质量和肿瘤体积(P<0.05,P<0.001)。Western blot结果显示,DDIE能明显降低CT26、HCT116细胞中MDM2蛋白表达水平(P<0.05,P<0.01)、增加p53蛋白表达水平(P<0.01,P<0.001),并能明显降低小鼠肿瘤组织MDM2蛋白表达水平(P<0.05)、增加p53蛋白表达水平(P<0.01)。结论DDIE具有抗结直肠癌作用,其机制与上调p53蛋白表达及抑制肿瘤细胞增殖和迁移、促进肿瘤细胞凋亡相关。

Changes in Blood and Semen Quality of Bulls after Artificial Infection with Mycoplasma wenyonii

[Objective]This study aimed to investigate the effects of Mycoplasma wenyoni on blood and semen quality of breeding bul s. [Method] Ten 10 healthy breeding bul s of Luxi cattle were randomly divided into two groups. Breeding bul s in experimental group were injected intravenously with M. wenyoni on Day 0 and Day 28. Effects of Mycoplasma wenyoni on breeding bul s were evaluated compre-hensively based on clinical symptoms, blood smears, blood physiological and bio-chemical properties, semen quality and scrotal changes. [Result] In addition to indi-vidual indices, the experimental group exhibited basical y normal clinical examination results without significant abnormal blood and semen quality. [Conclusion] This study suggests that M. wenyoni has low virulence and pathogenicity to breeding bul s af-ter artificial infection, leading to no significant changes in blood and semen quality.

运输过程中振动对猪精液质量影响的研究进展

在生产实践中,精液通常通过物流运输方式送达养殖场,但是在运输过程中车辆振动会对猪精子质量造成影响,甚至会损伤精子,进而降低人工授精的成功率。目前尚未建立精液运输过程监测系统,因此如何避免运输过程中振动对精子的损伤是提高养殖效益的关键因素之一。文章对精液运输过程中振动的产生及其对精液质量的影响研究进行了综述,旨在为提升运输后的精液质量提供参考依据。

精索静脉曲张超声分度在睾丸功能评估中的应用价值

目的探讨精索静脉曲张(VC)超声分度在睾丸功能评估中的应用价值。方法选取2021年7月至2022年12月于四川省人民医院就诊的152例VC患者为研究对象,根据VC严重程度将其分为轻度组(n=47)、中度组(n=51)和重度组(n=54)。通过超声测量3组精索静脉内径、反流时间及睾丸尺寸,计算睾丸容积和萎缩指数(AI),比较3组年龄、体重指数(BMI)、吸烟史、饮酒史、睾丸尺寸、精液、睾酮及血清卵泡刺激素(FSH),采用多个独立样本秩和检验、多因素Logistic回归分析不同VC超声分度与睾丸功能的相关性。结果3组年龄、BMI比较,差异有统计学意义(P<0.05)。3组睾丸AI、精子浓度、精子活力、血清FSH、睾酮比较,差异有统计学意义(P<0.05)。随着VC程度加重,3组睾丸AI和血清FSH的异常率均呈升高趋势。控制BMI、吸烟、饮酒和年龄等因素后,轻度组患病风险较重度组低,差异有统计学意义(P<0.05),重度组患病风险与中度组比较,差异无统计学意义(P>0.05)。结论VC超声分度与睾丸功能变化密切相关,超声分度可作为睾丸功能的评估指标。

不同抗氧化剂对体外保存的鸡精子活力及人工授精效果的影响

为研制适合鸡精液的高效稀释保存液,试验在前期筛选的两种效果较好配方的基础上,进行淫羊藿苷、半胱氨酸和绿原酸三种抗氧化剂的筛选,然后用筛选的稀释液进行人工授精试验。结果显示:抗氧化剂筛选试验中,添加半胱氨酸的A配方的精子保存效果最好。48 h时,A配方的精子平均活力为0.50,其精子质膜完整率和总抗氧化能力(T-AOC)浓度均显著高于其他配方(P<0.05),而精子畸形率显著低于其他配方(P<0.05)。鸡场授精试验中,稀释精液保存0 h时,3个比例稀释组的种蛋受精率、出苗率和健雏率与原精输精组无显著差异(P>0.05);8 h时,1∶3比例的种蛋受精率(83.50%)显著低于1∶1(93.60%)和1∶2(92.94%)(P<0.05),但各稀释比例的出雏率和健雏率均无明显差异(P>0.05);24 h时,各稀释比例的种蛋受精率、出雏率和健雏率均明显下降,1∶1比例组种蛋受精率显著高于1∶2和1∶3比例(P<0.05),1∶1和1∶2比例组的出雏率和健雏率均显著高于1∶3比例(P<0.05),而1∶1和1∶2比例组之间的出雏率和健雏率均无显著差异(P>0.05)。结果表明:添加半胱氨酸的A配方精子稀释液保存效果较好,使用自研稀释液与精液按1∶1和1∶2稀释比例体外保存鸡的精液,在0 h和8 h时输精可获得较好的人工授精效果。