Objective: The objective is to explore the effect of Cangfudaotan Tang on expression of organic anion transporting polypeptide (oatp4a1) in ovary and uterus tissues in rats with obese polycystic ovary syndrome. Method...Objective: The objective is to explore the effect of Cangfudaotan Tang on expression of organic anion transporting polypeptide (oatp4a1) in ovary and uterus tissues in rats with obese polycystic ovary syndrome. Methods: Poresky method and high fat diet were used to build obese PCOS rat models. The PCOS rat models were randomized into model control group, metformin group, the low dose Cangfudaotan Tang group and the high dose Cangfudaotan Tang group with 10 rats in each group. The metformin group was treated with metformin 43 mg/100g intragastric administration for two weeks, and the high dose Cangfudaotan Tang group and the low dose Cangfudaotan Tang group were given Cangfudaotan Tang 5.68 g/kg/d and 1.42 g/kg/d intragastric administration respectively for two weeks. Then the rats were anesthetized. Each rat was taken ovary and uterus tissues, and the expression of oatp4a1 Protein was detected in the tissues by using Western Blot techniques. The level of sex hormones as testosterone (T), follicle stimulating hormone (FSH), estradiol (E2), and luteinizing hormone (LH) and insulin resistance were detected by ELLISA techniques. Results: The expression of oatp4a1 in rat ovary and uterus tissue of model group was down-regulated compared to blank control group (p ≤ 0.05). The expression of oatp4a1 in ovary and uterus tissue of treatment group was up-regulated compared to model group (p < 0.05). The metformin group and the high does Cangfudaotan group were more apparent, while there was no statistical significance between the metformin group and the high dose Cangfudaotan group. Moreover, the model PCOS group had higher serum T, LH/FSH (p < 0.01) and lower E2 level (p < 0.01) compared to normal group. After treatment, serum T, LH (p < 0.01) and LH/FSH (P < 0.01) were decreased in metformin group and Cangfudaotan Tang group (p < 0.01) compared with those in PCOS model group. However, there was no significantly difference in serum T, LH, LH/FSH and E2 between metformin group and the high dose Cangfudaotan Tang group. Conclusion: The mechanism of Cangfudaotan Tang treatment of obese PCOS may be correlated to oatp4a1 by transportation and transformation of phlegmy dampness and regulating the levels of sex hormone.展开更多
目的:观察宽带汤联合西药治疗慢性盆腔炎的疗效及其对高迁移率族蛋白B1(high mobility group protein B1,HMGB-1)、可溶性晚期糖基化终末产物受体(soluble advanced glycation end product receptor,s RAGE)和Toll样受体-4(toll like re...目的:观察宽带汤联合西药治疗慢性盆腔炎的疗效及其对高迁移率族蛋白B1(high mobility group protein B1,HMGB-1)、可溶性晚期糖基化终末产物受体(soluble advanced glycation end product receptor,s RAGE)和Toll样受体-4(toll like receptor-4,TLR-4)通路的影响。方法:将慢性盆腔炎患者104例按照随机数字表法分为观察组和对照组各52例。对照组予常规抗炎治疗,观察组在对照组基础上予宽带汤治疗。观察两组患者治疗后疗效和复发率,观察两组患者治疗前后中医证候积分、平均月经量、平均月经经期、疼痛视觉模拟评分(visual analogue scale,VAS),观察两组患者全血高切、低切黏度,血细胞比容、红细胞沉降率及HMGB-1、sRAGE和TLR-4水平。结果:总有效率观察组为94.2%(49/52),高于对照组的76.9%(40/52),差异有统计学意义(χ^2=4.986,P<0.05)。复发率观察组为1.9%(1/52),低于对照组的15.4%(8/52)(χ^2=4.379,P<0.05)。治疗后两组患者全血高切、低切黏度及血细胞比容、红细胞沉降率均较治疗前下降,观察组低于对照组(P<0.01)。治疗后两组患者中医证候积分、平均月经量、平均月经经期,VAS评分,HMGB-1和TLR-4水平均较治疗前降低(P<0.01),sRAGE水平较治疗前升高(P<0.01),观察组降低或升高较对照组明显(P<0.01)。结论:宽带汤联合西药治疗慢性盆腔炎疗效优于单纯西药治疗,且复发率低,其机制可能与通过HMGB-1、sRAGE和TLR-4通路减轻炎症反应有关。展开更多
文摘Objective: The objective is to explore the effect of Cangfudaotan Tang on expression of organic anion transporting polypeptide (oatp4a1) in ovary and uterus tissues in rats with obese polycystic ovary syndrome. Methods: Poresky method and high fat diet were used to build obese PCOS rat models. The PCOS rat models were randomized into model control group, metformin group, the low dose Cangfudaotan Tang group and the high dose Cangfudaotan Tang group with 10 rats in each group. The metformin group was treated with metformin 43 mg/100g intragastric administration for two weeks, and the high dose Cangfudaotan Tang group and the low dose Cangfudaotan Tang group were given Cangfudaotan Tang 5.68 g/kg/d and 1.42 g/kg/d intragastric administration respectively for two weeks. Then the rats were anesthetized. Each rat was taken ovary and uterus tissues, and the expression of oatp4a1 Protein was detected in the tissues by using Western Blot techniques. The level of sex hormones as testosterone (T), follicle stimulating hormone (FSH), estradiol (E2), and luteinizing hormone (LH) and insulin resistance were detected by ELLISA techniques. Results: The expression of oatp4a1 in rat ovary and uterus tissue of model group was down-regulated compared to blank control group (p ≤ 0.05). The expression of oatp4a1 in ovary and uterus tissue of treatment group was up-regulated compared to model group (p < 0.05). The metformin group and the high does Cangfudaotan group were more apparent, while there was no statistical significance between the metformin group and the high dose Cangfudaotan group. Moreover, the model PCOS group had higher serum T, LH/FSH (p < 0.01) and lower E2 level (p < 0.01) compared to normal group. After treatment, serum T, LH (p < 0.01) and LH/FSH (P < 0.01) were decreased in metformin group and Cangfudaotan Tang group (p < 0.01) compared with those in PCOS model group. However, there was no significantly difference in serum T, LH, LH/FSH and E2 between metformin group and the high dose Cangfudaotan Tang group. Conclusion: The mechanism of Cangfudaotan Tang treatment of obese PCOS may be correlated to oatp4a1 by transportation and transformation of phlegmy dampness and regulating the levels of sex hormone.
文摘目的:观察宽带汤联合西药治疗慢性盆腔炎的疗效及其对高迁移率族蛋白B1(high mobility group protein B1,HMGB-1)、可溶性晚期糖基化终末产物受体(soluble advanced glycation end product receptor,s RAGE)和Toll样受体-4(toll like receptor-4,TLR-4)通路的影响。方法:将慢性盆腔炎患者104例按照随机数字表法分为观察组和对照组各52例。对照组予常规抗炎治疗,观察组在对照组基础上予宽带汤治疗。观察两组患者治疗后疗效和复发率,观察两组患者治疗前后中医证候积分、平均月经量、平均月经经期、疼痛视觉模拟评分(visual analogue scale,VAS),观察两组患者全血高切、低切黏度,血细胞比容、红细胞沉降率及HMGB-1、sRAGE和TLR-4水平。结果:总有效率观察组为94.2%(49/52),高于对照组的76.9%(40/52),差异有统计学意义(χ^2=4.986,P<0.05)。复发率观察组为1.9%(1/52),低于对照组的15.4%(8/52)(χ^2=4.379,P<0.05)。治疗后两组患者全血高切、低切黏度及血细胞比容、红细胞沉降率均较治疗前下降,观察组低于对照组(P<0.01)。治疗后两组患者中医证候积分、平均月经量、平均月经经期,VAS评分,HMGB-1和TLR-4水平均较治疗前降低(P<0.01),sRAGE水平较治疗前升高(P<0.01),观察组降低或升高较对照组明显(P<0.01)。结论:宽带汤联合西药治疗慢性盆腔炎疗效优于单纯西药治疗,且复发率低,其机制可能与通过HMGB-1、sRAGE和TLR-4通路减轻炎症反应有关。
