Gladiolus hybridus Hort is one of the world＇s famous cutting flowers. It is very popular because of the big size, bright color, various shape, and long period of bloom. New species should be cultivated in order to me...Gladiolus hybridus Hort is one of the world＇s famous cutting flowers. It is very popular because of the big size, bright color, various shape, and long period of bloom. New species should be cultivated in order to meet the consumers＇ need of asking for the new. Among the technologies of cultivating new species of flowers, mutagenic breeding is a shortcut. This study treated corm bud of G. hybridus Hort with EMS of different consistency. Then M1 after treated was analyzed by RAPD. The result showed that EMS was a very effective mutagenic agent for the corm bud of G. hybridus Hort. With the increase of consistency, the mutagenic range increased first, then decreased, among which 0.6% EMS treatment had the biggest influence. However, with the same EMS consistency, there was not close relevancy between the amount of mutagenic agent and the divergence of plant＇s genomes, which offered a molecular basis for selecting plants with good mutation.展开更多
Protoplasts prepared from the aseptic leaves of Rose Supreme in Gladiolus hybridus Hort. were cultured. The yield of protoplasts with vigour was the highest under the following conditions： 1.5% cellulase Onzuka RS, 0...Protoplasts prepared from the aseptic leaves of Rose Supreme in Gladiolus hybridus Hort. were cultured. The yield of protoplasts with vigour was the highest under the following conditions： 1.5% cellulase Onzuka RS, 0.5% pectinase Y-23, 0.6 mol· L^-1 mannitol, pH 5.8, digestion time 2 h, and temperature 27℃. After isolation and purification, protoplasts were cultured in solid and liquid combined medium, which included KM8P, 0.6 mol· L^-1 mannitol, 1.0 mg· L^-1 2, 4-D, 0.2 mg· L^-1 NAA and 0.2 mg· L^-1 KT. The division of the protoplasts first emerged in medium after cultured 4 days, and emerged only a few times.展开更多
Gladiolus hybridus Hort is one of famous cutting flowers,being famous of big size,bright color,various shapes and long bloom period.New species should be cultivated in order to meet consumers needs.Mutagenic breeding ...Gladiolus hybridus Hort is one of famous cutting flowers,being famous of big size,bright color,various shapes and long bloom period.New species should be cultivated in order to meet consumers needs.Mutagenic breeding is a shortcut to cultivate new species of flowers.In this study,corm bud of G.hybridus Hort was treated with different concentrations of EMS.Then M 1 generation was analyzed by ISSR.Results showed that EMS was a very effective mutagenic agent for the corm bud of G.hybridus Hort.With the increa...展开更多
ISSR analysis is applied in this study to research the classification and genetic relationship of 26 cultivars of G.hybridus Hort.Total 19 arbitrary primers screened from 100 primers were used for further PCR and dive...ISSR analysis is applied in this study to research the classification and genetic relationship of 26 cultivars of G.hybridus Hort.Total 19 arbitrary primers screened from 100 primers were used for further PCR and diversity analysis.A total of 110 ISSR sites were detected with a mean of 5.63 fragments amplified for each primer.A total of 103 polymorphic DNA fragments were detected among all the 110 amplified fragments,which accounted for a high level of 93.6%of all and could be used to identify different cultivars.The results revealed that the idioplasm resource of G.hybridus Hort cutting flower cultivar had a narrow genetic basis on molecular level,and certain genetic relationship existed in summer large-flowers of G.hybridus Hort varieties.展开更多
Two new compounds, senarguines A and B, were isolated from Senecio argunensis. They were respectively identified as （Z）-3- ethylidene-9-hydroxyl-9-methyl-6,7-dioxaspiro [4.4] nonane-5,8-dione （1） and 3,6,6-trimeth...Two new compounds, senarguines A and B, were isolated from Senecio argunensis. They were respectively identified as （Z）-3- ethylidene-9-hydroxyl-9-methyl-6,7-dioxaspiro [4.4] nonane-5,8-dione （1） and 3,6,6-trimethyl 6,7,8-tetrahydronaphtha-[1,2b] furan （2） on the basis of spectral data.展开更多
A new Eremophilanoid sesquiterpene (1) was isolated from the whole plant of Senecio oldhamianus. Its structure was elucidated as 7? 11-epoxy-9? 10?epoxy-8-oxoeremophilane using spectroscopic methords and X-ray analysis.
Objective:To investigate activity-guided isolation and identification of anti-Staphylococcus aures components from Senecio tenuifolius Burm.F.(S.tenuifolius).Methods:Hexane,chloroform,ethyl acetate,methanol and aqueou...Objective:To investigate activity-guided isolation and identification of anti-Staphylococcus aures components from Senecio tenuifolius Burm.F.(S.tenuifolius).Methods:Hexane,chloroform,ethyl acetate,methanol and aqueous extracts of S.tenuifolius were prepared by soxilation for antimicrobial activity against one registered Staphylococcus aureus(S.aureus)(ATCC No:25923)and two clinical isolates,methicillin resistant and methicillin sensitive S.aureus.NCCL standard methods were followed for antibacterial activity.GC-MS was performed to identify the chemical composition of bio active fraction.Results:Among all solvent extracts,methanol extract significantly reduced the growth of S.aureus(ATCC No:25923),methicillin resistant and methicillin sensitive S.aureus with the best zone of inhibition at 16.23,14.06 and 15.23mm and minimum inhibition concentration(MIC)values at 426.16,683.22 and 512.12μg/mL,respectively.In order to detect the active component in methanol extract,it was further purified by column chromatography,which yielded four fractions(St1,St2,St3,and St4).Among these four fractions,St3 was effective against the tested strains of S.aures,with the best zone of inhibition at 15.09,13.25 and 14.12 mm and with best MIC values at 88.16,128.11 and 116.12μg/mL,respectively.Effective fraction partially purified from S.tenuifolius(St3)yielded MIC's that were at least 20 fold less when compared to crude extract.GC-MS analysis of St3 revealed the presence of 3-[methyl-6,7-dihydro benzofuran-4(5H)-one],1,2-benzenedicarboxylic acid,hydroquinone,methyl ester and 3 unknown compounds.Conclusions:The study provides scientific evidence for traditional and folklore medicinal use of S.tenuifolius in skin infections treatment.展开更多
基金Supported by Postdoctoral Fund of China (20080430871)Postdoctoral Funds Project of Heilongjiang Province+1 种基金Innovation Talent Research Fund of Harbin City (2006RFLXN007)Education Project Fund of Heilongjiang Province (11511037)
文摘Gladiolus hybridus Hort is one of the world＇s famous cutting flowers. It is very popular because of the big size, bright color, various shape, and long period of bloom. New species should be cultivated in order to meet the consumers＇ need of asking for the new. Among the technologies of cultivating new species of flowers, mutagenic breeding is a shortcut. This study treated corm bud of G. hybridus Hort with EMS of different consistency. Then M1 after treated was analyzed by RAPD. The result showed that EMS was a very effective mutagenic agent for the corm bud of G. hybridus Hort. With the increase of consistency, the mutagenic range increased first, then decreased, among which 0.6% EMS treatment had the biggest influence. However, with the same EMS consistency, there was not close relevancy between the amount of mutagenic agent and the divergence of plant＇s genomes, which offered a molecular basis for selecting plants with good mutation.
基金Supported by Heilongjiang Natural Sciences of Fundation (C2004-01)
文摘Protoplasts prepared from the aseptic leaves of Rose Supreme in Gladiolus hybridus Hort. were cultured. The yield of protoplasts with vigour was the highest under the following conditions： 1.5% cellulase Onzuka RS, 0.5% pectinase Y-23, 0.6 mol· L^-1 mannitol, pH 5.8, digestion time 2 h, and temperature 27℃. After isolation and purification, protoplasts were cultured in solid and liquid combined medium, which included KM8P, 0.6 mol· L^-1 mannitol, 1.0 mg· L^-1 2, 4-D, 0.2 mg· L^-1 NAA and 0.2 mg· L^-1 KT. The division of the protoplasts first emerged in medium after cultured 4 days, and emerged only a few times.
基金Supported by Heilongjiang Provincial Program for Youth Science (QC07C51)China Postdoctoral Foundation (20080430871)Program for Innovative Research Team of Northeast Agricultural University (CX2004-2)
文摘Gladiolus hybridus Hort is one of famous cutting flowers,being famous of big size,bright color,various shapes and long bloom period.New species should be cultivated in order to meet consumers needs.Mutagenic breeding is a shortcut to cultivate new species of flowers.In this study,corm bud of G.hybridus Hort was treated with different concentrations of EMS.Then M 1 generation was analyzed by ISSR.Results showed that EMS was a very effective mutagenic agent for the corm bud of G.hybridus Hort.With the increa...
基金Supported by Postdoctoral Fund of China(20080430871)Postdoctoral Funds Project of Heilongjiang Province(520-415037)Innovation Talent Research Fund of Harbin City(2006RFLXN007)
文摘ISSR analysis is applied in this study to research the classification and genetic relationship of 26 cultivars of G.hybridus Hort.Total 19 arbitrary primers screened from 100 primers were used for further PCR and diversity analysis.A total of 110 ISSR sites were detected with a mean of 5.63 fragments amplified for each primer.A total of 103 polymorphic DNA fragments were detected among all the 110 amplified fragments,which accounted for a high level of 93.6%of all and could be used to identify different cultivars.The results revealed that the idioplasm resource of G.hybridus Hort cutting flower cultivar had a narrow genetic basis on molecular level,and certain genetic relationship existed in summer large-flowers of G.hybridus Hort varieties.
基金partially supported by a grant from the National High Technology Research and Development Program of PR China(863 Program)(No.2004AA2Z3730-15).
文摘Two new compounds, senarguines A and B, were isolated from Senecio argunensis. They were respectively identified as （Z）-3- ethylidene-9-hydroxyl-9-methyl-6,7-dioxaspiro [4.4] nonane-5,8-dione （1） and 3,6,6-trimethyl 6,7,8-tetrahydronaphtha-[1,2b] furan （2） on the basis of spectral data.
文摘From Senecio saluenensis, a novel eremophilanolide Saluenolide A was isolated. Its structure was elucidated by 2D-NMR technique and X-ray diffraction.
基金This work was financially supported by the National Nature Science Foundation of China (NO. 29972017) and the Foundation of Ministry of Education of China for Doctoral Program (NO. 98073003).
文摘A new Eremophilanoid sesquiterpene (1) was isolated from the whole plant of Senecio oldhamianus. Its structure was elucidated as 7? 11-epoxy-9? 10?epoxy-8-oxoeremophilane using spectroscopic methords and X-ray analysis.
基金Supported by the Department of Riotechnology.Government of Indiafor funding(DBT-Sanction No.102/IFD/SAN/PR0313)
文摘Objective:To investigate activity-guided isolation and identification of anti-Staphylococcus aures components from Senecio tenuifolius Burm.F.(S.tenuifolius).Methods:Hexane,chloroform,ethyl acetate,methanol and aqueous extracts of S.tenuifolius were prepared by soxilation for antimicrobial activity against one registered Staphylococcus aureus(S.aureus)(ATCC No:25923)and two clinical isolates,methicillin resistant and methicillin sensitive S.aureus.NCCL standard methods were followed for antibacterial activity.GC-MS was performed to identify the chemical composition of bio active fraction.Results:Among all solvent extracts,methanol extract significantly reduced the growth of S.aureus(ATCC No:25923),methicillin resistant and methicillin sensitive S.aureus with the best zone of inhibition at 16.23,14.06 and 15.23mm and minimum inhibition concentration(MIC)values at 426.16,683.22 and 512.12μg/mL,respectively.In order to detect the active component in methanol extract,it was further purified by column chromatography,which yielded four fractions(St1,St2,St3,and St4).Among these four fractions,St3 was effective against the tested strains of S.aures,with the best zone of inhibition at 15.09,13.25 and 14.12 mm and with best MIC values at 88.16,128.11 and 116.12μg/mL,respectively.Effective fraction partially purified from S.tenuifolius(St3)yielded MIC's that were at least 20 fold less when compared to crude extract.GC-MS analysis of St3 revealed the presence of 3-[methyl-6,7-dihydro benzofuran-4(5H)-one],1,2-benzenedicarboxylic acid,hydroquinone,methyl ester and 3 unknown compounds.Conclusions:The study provides scientific evidence for traditional and folklore medicinal use of S.tenuifolius in skin infections treatment.
