In this paper, the asynchrony problem of distributed detection is analyzed and discussed.Two approaches are proposed and related results are given. It is shown that all fusion rules can beunified in the framework with...In this paper, the asynchrony problem of distributed detection is analyzed and discussed.Two approaches are proposed and related results are given. It is shown that all fusion rules can beunified in the framework with asynchrony which could be much ciooer to industrial practice.展开更多
Marine in situ testing is a necessary step for stereotyping newly developed marine sensors. The use of test sites in the Yangtze Estuary area, which has high turbidity and abundant nutrients, can effectively reduce th...Marine in situ testing is a necessary step for stereotyping newly developed marine sensors. The use of test sites in the Yangtze Estuary area, which has high turbidity and abundant nutrients, can effectively reduce the needed testing time owing to its harsh conditions. Five test stations were established, and a floating buoy and fixed test equipment were designed. A control system, including a sensor connection, data processor, video remote transmission, and corresponding control algorithm, was developed. The control system enabled the nondestructive monitoring of biological attachments and bidirectional, real-time communication between an upper server on land and the control system at the test sites. The dissolved oxygen(DO), temperature, and pH data of DOS600 and DPS600 sensors were compared with those of AP2000 sensors. Temperature recording using the DOS600 sensor was performed nearly as well as that of the AP2000 sensor. The mean DO values(standard deviations) were 8.414 mg L-1(2.068) and 6.896 mg L-1(1.235) for the DOS600 and AP2000 sensors, respectively, indicating that the DOS600 performance was unsatisfactory. The pH recording of the DPS600 was slightly worse than that of the AP2000 sensor. Experimental results showed that the DO value was more easily affected by the buoy movement of waves compared to the pH and temperature. Moreover, data fluctuations showed that the DO and pH parameters were more vulnerable to biofouling than temperature. Waves and biofouling create a harsh test environment, and the performance difference between the developed sensors and a standard sensor can be obtained in a short time period.展开更多
A fully distributed optical fiber sensor (DOFS) for monitoring long-distance oil pipeline health is proposed based on optical time domain reflectometry (OTDR). A smart and sensitive optical fiber cable is installe...A fully distributed optical fiber sensor (DOFS) for monitoring long-distance oil pipeline health is proposed based on optical time domain reflectometry (OTDR). A smart and sensitive optical fiber cable is installed along the pipeline acting as a sensor, The experiments show that the cable swells when exposed to oil and induced additional bending losses inside the fiber, and the optical attenuation of the fiber coated by a thin skin with periodical hardness is sensitive to deformation and vibration caused by oil leakage, tampering, or mechanical impact. The region where the additional attenuation occurred is detected and located by DOFS based on OTDR, the types of pipeline accidents are identified according to the characteristics of transmitted optical power received by an optical power meter, Another prototype of DOFS based on a forward traveling frequency-modulated continuous-wave (FMCW) is also proposed to monitor pipeline. The advantages and disadvantages of DOFSs based on OTDR and FMCW are discussed. The experiments show that DOFSs are capable of detecting and locating distant oil pipeline leakages and damages in real time with an estimated precision of ten meters over tens of kilometers.展开更多
A numerical simulation model of plenoptic sensor aberration wavefront detection is established to simulate and analyze the detection performance of plenoptic sensor aberration wavefront for different turbulence intens...A numerical simulation model of plenoptic sensor aberration wavefront detection is established to simulate and analyze the detection performance of plenoptic sensor aberration wavefront for different turbulence intensities.The results show that the plenoptic sensor can achieve better distortion wavefront detection,and its wavefront detection accuracy improves with turbulence intensity.The unique optical structure design of the plenoptic sensor makes it more suitable for aberration wavefront detection in strong turbulent conditions.The wavefront detection performance of the plenoptic sensor is not only related to its wavefront reconstruction algorithm but also closely related to its structural parameter settings.The influence of structural parameters on the wavefront detection accuracy of plenoptic sensors under different turbulence intensities is simulated and analyzed.The variation law of wavefront detection accuracy and structural parameters under different turbulence intensities is summarized to provide a reference for the structural design and parameter optimization of plenoptic sensors.展开更多
A pseudorotaxane and its polypseudorotaxanes formed between pillar[5]arene moieties and noctylpyrazinium cations as novel fluorescent sensors for the selective detection of halogen ions were reported.A collapse of the...A pseudorotaxane and its polypseudorotaxanes formed between pillar[5]arene moieties and noctylpyrazinium cations as novel fluorescent sensors for the selective detection of halogen ions were reported.A collapse of these pillar[5]arene-based pseudorotaxanes and polypseudorotaxanes occurred upon the addition of Cl,Br,and I(tetrabutylammonium salts),respectively,leading to their fluorescence recovery.The fluorescence enhancement of the pseudorotaxane and the polypseudorotaxanes increases in the order of I展开更多
A method of detecting dry, icy and wet road surface conditions based on scanniag detection of single wavelength backward power is proposed in this letter. The detector is used to receive the backward scattered power w...A method of detecting dry, icy and wet road surface conditions based on scanniag detection of single wavelength backward power is proposed in this letter. The detector is used to receive the backward scattered power which changes with the incidence angle. The relationship between backward power and incidence angle is used to find out the effective angle range and distinguish method. Experiment and simulation show that it is feasible to classifv these three conditions within incidence angle of 5.3 degree.展开更多
CdSe/CdS quantum dots (QDs) functionalized by thiourea (TU) were synthesized and used as a fluorescent sensor for mercury ion detection. The TU-functionalized QDs were prepared by bonding TU via electrostatic inte...CdSe/CdS quantum dots (QDs) functionalized by thiourea (TU) were synthesized and used as a fluorescent sensor for mercury ion detection. The TU-functionalized QDs were prepared by bonding TU via electrostatic interaction to the core/shell CdSe/CdS QDs after capping with thioglycolic acid (TGA). It was observed that the fluorescence of the functionalized QDs was quenched upon the addition of Hg^2+. The quantitative detection of Hg^2+ with this fluorescent sensor could be conducted based on the linear relationship between the extent of quenching and the concentration of Hg^2+ added in the range of 1-300 μg.L^-1, A detection limit of 0.56 μg.L^-1 was achieved. The sensor showed superior selectivity for Hg^2+ and was successfully applied to the determination of mercury in environmental samples with satisfactory results展开更多
An optical hydrogen sulfide(H_2S) sensor based on wavelength modulation spectroscopy with the second harmonic(2f) corrected by the first harmonic(1f) signal(WMS-2f/1f) is developed using a distributed feedback(DFB) la...An optical hydrogen sulfide(H_2S) sensor based on wavelength modulation spectroscopy with the second harmonic(2f) corrected by the first harmonic(1f) signal(WMS-2f/1f) is developed using a distributed feedback(DFB) laser emitting at 1.578 μm and a homemade gas cell with 1-m-long optical path length. The novel sensor is constructed by an electrical cabinet and an optical reflecting and receiving end. The DFB laser is employed for targeting a strong H_2S line at 6 336.62 cm^(-1) in the fundamental absorption band of H_2S. The sensor performance, including the minimum detection limit and the stability, can be improved by reducing the laser intensity drift and common mode noise by means of the WMS-2f/1f technique. The experimental results indicate that the linearity and response time of the sensor are 0.999 26 and 6 s(in concentration range of 15.2—45.6 mg/m^3), respectively. The maximum relative deviation for continuous detection(60 min) of 30.4 mg/m^3 H_2S is 0.48% and the minimum detection limit obtained by Allan variance is 79 μg/m^3 with optimal integration time of 32 s. The optical H_2S sensor can be applied to environmental monitoring and industrial production, and it has significance for real-time online detection in many fields.展开更多
We discuss the efficiency of an electro-optic (EO) polymer sensor with interdigitated coplanar electrodes. The developed EO sensor is used to detect terahertz radiation via EO sampling. Results show that the sensor ...We discuss the efficiency of an electro-optic (EO) polymer sensor with interdigitated coplanar electrodes. The developed EO sensor is used to detect terahertz radiation via EO sampling. Results show that the sensor improves more significantly detection sensitivity than does a sensor with sandwich configurations.展开更多
With the rapid development of human artificial intelligence and the inevitably expanding markets, the past two decades have witnessed an urgent demand for the flexible and wearable devices, especially the flexible str...With the rapid development of human artificial intelligence and the inevitably expanding markets, the past two decades have witnessed an urgent demand for the flexible and wearable devices, especially the flexible strain sensors. Flexible strain sensors, incorporated the merits of stretchability, high sensitivity and skin-mountable,are emerging as an extremely charming domain in virtue of their promising applications in artificial intelligent realms, human-machine systems and health-care devices. In this review, we concentrate on the transduction mechanisms, building blocks of flexible physical sensors, subsequently property optimization in terms of device structures and sensing materials in the direction of practical applications. Perspectives on the existing challenges are also highlighted in the end.展开更多
Wireless sensors are deployed widely to monitor space, emergent events, and disasters. Collected realtime sensory data are precious for completing rescue missions quickly and efficiently. Detecting isolate safe areas ...Wireless sensors are deployed widely to monitor space, emergent events, and disasters. Collected realtime sensory data are precious for completing rescue missions quickly and efficiently. Detecting isolate safe areas is significant for various applications of event and disaster monitoring since valuable real-time information can be provided for the rescue crew to save persons who are trapped in isolate safe areas. We propose a centralized method to detect isolate safe areas via discovering holes in event areas. In order to shorten the detection delay, a distributed isolate safe area detection method is studied. The distributed method detects isolate safe areas during the process of event detection. Moreover, detecting isolate safe areas in a building is addressed particularly since the regular detecting method is not applicable. Our simulation results show that the distributed method can detect all isolate safe areas in an acceptable short delay.展开更多
Compressors play an important role in day-to-day operation in most oil and gas platforms,especially in the case for maintaining gas pressure in transportation pipe.Its complex problem to detect the sensors health and ...Compressors play an important role in day-to-day operation in most oil and gas platforms,especially in the case for maintaining gas pressure in transportation pipe.Its complex problem to detect the sensors health and abnormality as the sensor reading would reflect the various states of the compressor.In ideal situation,sensor readings offer vast amounts of information on compressor health and could possibly indicate early fault of machines.Furthermore,due to harsh site and process operating conditions,sensors are often found to have drifted or failed,and there is no standard methodology to predict abnormality apart from applying emerging industrial smart sensor technologies.In this paper,we investigate a minimalist approach for detecting abnormality of compressor's shaft's RPM sensor.As the sensors in the compressor are correlated,we first use the outputs of other sensors to predict the shaft's RPM using regression-based models(neural networks and multiple linear regression).Second,we calculate the histogram of residuals by taking the difference between the predicted sensor value and the actual sensor value plus the abnormality in terms of bias/miscalibration and noise.The histogram of residuals can be used for sensor abnormality monitoring.In general,sensor states can be monitored by observing the shifting of the mean in the histogram of residuals.The sensor readings contaminated with noise can be seen by a shifted mean whose value is between the normal condition mean and the biased condition mean.This method is compact and would be relevant to monitor irregularity of the sensors.展开更多
A new fluorescence sensor is developed for simultaneous detection of Hg^2+ and Ag^+. During the detection process,Hg^2+ forms complexes with the fluorescent dye rhodamine B isothiocyanate(RBITC) modified onto the...A new fluorescence sensor is developed for simultaneous detection of Hg^2+ and Ag^+. During the detection process,Hg^2+ forms complexes with the fluorescent dye rhodamine B isothiocyanate(RBITC) modified onto the surface of gold nanoparticles(Au NPs),resulting in RBITC's displacement from the surface of Au NPs and the recovery of fluorescence. Meanwhile,Ag^+ forms "C-Ag^+-C" complex with C-rich 5-carboxyfluorescein(FAM)-ss DNA modified onto the surface of Au NPs,which keeps the fluorescent dye FAM close to the Au NPs and results in quench of fluorescence. The experimental results show a wide linear range and a good sensitivity. The limit of detection is 1.06 nmol/L for Ag^+ and 0.48 nmol/L for Hg^2+. This detection method is not only easy to operate but also efficient.展开更多
In this work, the application of a conducting polymer, poly(thionine), modified electrode as matrix to DNA immobilization as well as transducer to label-free DNA hybridization detection was introduced. The electropo...In this work, the application of a conducting polymer, poly(thionine), modified electrode as matrix to DNA immobilization as well as transducer to label-free DNA hybridization detection was introduced. The electropolymerization of thionine onto electrode surface was carried out by a simple two-step method, which involved a preanodization of glassy carbon electrode at a constant positive potential in thionine solution following cyclic voltammetry scans in the solution. Electrochemical detection was performed by differential pulse voltammetry in the electroactivity potential domain of poly(thionine). The resulting poly(thionine) modified electrode showed a good stability and electroactivity in aqueous media during a near neutral pH range. Additionally, the pendant amino groups on the poly(thionine) chains enabled poly(thionine) modified electrode to immobilize phosphate group terminated DNA probe via covalent linkage. Hybridization process induced a clear decrease in poly(thionine) redox current, which was corresponding to the decrease in poly(thionine) electroactivity after double stranded DNA was formed on the polymer film. The detection limit of this electrochemical DNA hybridization sensor was 1.0 × 10^-10mol/L. Compared with complementary sequence, the hybridization signal values of 1-base mismatched and 3-base mismatched samples were 63.9% and 9.2%, respectively.展开更多
In this study,a multi-object tracking(MOT)scheme based on a light detection and ranging sensor was proposed to overcome imprecise velocity observations in object occlusion scenarios.By applying real-time velocity esti...In this study,a multi-object tracking(MOT)scheme based on a light detection and ranging sensor was proposed to overcome imprecise velocity observations in object occlusion scenarios.By applying real-time velocity estimation,a modified unscented Kalman filter(UKF)was proposed for the state estimation of a target object.The proposed method can reduce the calculation cost by obviating unscented transformations.Additionally,combined with the advantages of a two-reference-point selection scheme based on a center point and a corner point,a reference point switching approach was introduced to improve tracking accuracy and consistency.The state estimation capability of the proposed UKF was verified by comparing it with the standard UKF in single-target tracking simulations.Moreover,the performance of the proposed MOT system was evaluated using real traffic datasets.展开更多
文摘In this paper, the asynchrony problem of distributed detection is analyzed and discussed.Two approaches are proposed and related results are given. It is shown that all fusion rules can beunified in the framework with asynchrony which could be much ciooer to industrial practice.
基金supported by the National Key Research and Development Plan(No.2019YFD0901300)the Shanghai Science and Technology Innovation Action Plan(No.16DZ1205100)the Shanghai Agriculture Applied Technology Development Program(No.T20180303)。
文摘Marine in situ testing is a necessary step for stereotyping newly developed marine sensors. The use of test sites in the Yangtze Estuary area, which has high turbidity and abundant nutrients, can effectively reduce the needed testing time owing to its harsh conditions. Five test stations were established, and a floating buoy and fixed test equipment were designed. A control system, including a sensor connection, data processor, video remote transmission, and corresponding control algorithm, was developed. The control system enabled the nondestructive monitoring of biological attachments and bidirectional, real-time communication between an upper server on land and the control system at the test sites. The dissolved oxygen(DO), temperature, and pH data of DOS600 and DPS600 sensors were compared with those of AP2000 sensors. Temperature recording using the DOS600 sensor was performed nearly as well as that of the AP2000 sensor. The mean DO values(standard deviations) were 8.414 mg L-1(2.068) and 6.896 mg L-1(1.235) for the DOS600 and AP2000 sensors, respectively, indicating that the DOS600 performance was unsatisfactory. The pH recording of the DPS600 was slightly worse than that of the AP2000 sensor. Experimental results showed that the DO value was more easily affected by the buoy movement of waves compared to the pH and temperature. Moreover, data fluctuations showed that the DO and pH parameters were more vulnerable to biofouling than temperature. Waves and biofouling create a harsh test environment, and the performance difference between the developed sensors and a standard sensor can be obtained in a short time period.
基金This project is supported by R&D Foundation of National Petroleum Corporation (CNPC) of China(No.2001411-4).
文摘A fully distributed optical fiber sensor (DOFS) for monitoring long-distance oil pipeline health is proposed based on optical time domain reflectometry (OTDR). A smart and sensitive optical fiber cable is installed along the pipeline acting as a sensor, The experiments show that the cable swells when exposed to oil and induced additional bending losses inside the fiber, and the optical attenuation of the fiber coated by a thin skin with periodical hardness is sensitive to deformation and vibration caused by oil leakage, tampering, or mechanical impact. The region where the additional attenuation occurred is detected and located by DOFS based on OTDR, the types of pipeline accidents are identified according to the characteristics of transmitted optical power received by an optical power meter, Another prototype of DOFS based on a forward traveling frequency-modulated continuous-wave (FMCW) is also proposed to monitor pipeline. The advantages and disadvantages of DOFSs based on OTDR and FMCW are discussed. The experiments show that DOFSs are capable of detecting and locating distant oil pipeline leakages and damages in real time with an estimated precision of ten meters over tens of kilometers.
基金the National Natural Science Foundation of China(No.61605223)the Strategic Priority Research Program of Chinese Academy of Sciences(No.614A010717)the Director Fund of Advanced Laser Technology Laboratory of Anhui Province(No.AHL2021ZR06)。
文摘A numerical simulation model of plenoptic sensor aberration wavefront detection is established to simulate and analyze the detection performance of plenoptic sensor aberration wavefront for different turbulence intensities.The results show that the plenoptic sensor can achieve better distortion wavefront detection,and its wavefront detection accuracy improves with turbulence intensity.The unique optical structure design of the plenoptic sensor makes it more suitable for aberration wavefront detection in strong turbulent conditions.The wavefront detection performance of the plenoptic sensor is not only related to its wavefront reconstruction algorithm but also closely related to its structural parameter settings.The influence of structural parameters on the wavefront detection accuracy of plenoptic sensors under different turbulence intensities is simulated and analyzed.The variation law of wavefront detection accuracy and structural parameters under different turbulence intensities is summarized to provide a reference for the structural design and parameter optimization of plenoptic sensors.
基金the financial support of the National Natural Science Foundation of China (No. 21202083)Natural Science Foundation of Jiangsu (Nos. BK2011055, BK2011551)the China Postdoctoral Science Foundation (No. 2012M511717)
文摘A pseudorotaxane and its polypseudorotaxanes formed between pillar[5]arene moieties and noctylpyrazinium cations as novel fluorescent sensors for the selective detection of halogen ions were reported.A collapse of these pillar[5]arene-based pseudorotaxanes and polypseudorotaxanes occurred upon the addition of Cl,Br,and I(tetrabutylammonium salts),respectively,leading to their fluorescence recovery.The fluorescence enhancement of the pseudorotaxane and the polypseudorotaxanes increases in the order of I
文摘A method of detecting dry, icy and wet road surface conditions based on scanniag detection of single wavelength backward power is proposed in this letter. The detector is used to receive the backward scattered power which changes with the incidence angle. The relationship between backward power and incidence angle is used to find out the effective angle range and distinguish method. Experiment and simulation show that it is feasible to classifv these three conditions within incidence angle of 5.3 degree.
基金the financial support from the National Natural Science Foundation of China (Nos. 20345006 and 20575043)
文摘CdSe/CdS quantum dots (QDs) functionalized by thiourea (TU) were synthesized and used as a fluorescent sensor for mercury ion detection. The TU-functionalized QDs were prepared by bonding TU via electrostatic interaction to the core/shell CdSe/CdS QDs after capping with thioglycolic acid (TGA). It was observed that the fluorescence of the functionalized QDs was quenched upon the addition of Hg^2+. The quantitative detection of Hg^2+ with this fluorescent sensor could be conducted based on the linear relationship between the extent of quenching and the concentration of Hg^2+ added in the range of 1-300 μg.L^-1, A detection limit of 0.56 μg.L^-1 was achieved. The sensor showed superior selectivity for Hg^2+ and was successfully applied to the determination of mercury in environmental samples with satisfactory results
基金supported by the National Natural Science Foundation of China(Nos.60808020 and 61078041)the Natural Science Foundation of Tianjin(Nos.16JCQNJC02100,15JCYBJC51700 and 16JCYBJC15400)the National Science and Technology Support(No.2014BAH03F01)
文摘An optical hydrogen sulfide(H_2S) sensor based on wavelength modulation spectroscopy with the second harmonic(2f) corrected by the first harmonic(1f) signal(WMS-2f/1f) is developed using a distributed feedback(DFB) laser emitting at 1.578 μm and a homemade gas cell with 1-m-long optical path length. The novel sensor is constructed by an electrical cabinet and an optical reflecting and receiving end. The DFB laser is employed for targeting a strong H_2S line at 6 336.62 cm^(-1) in the fundamental absorption band of H_2S. The sensor performance, including the minimum detection limit and the stability, can be improved by reducing the laser intensity drift and common mode noise by means of the WMS-2f/1f technique. The experimental results indicate that the linearity and response time of the sensor are 0.999 26 and 6 s(in concentration range of 15.2—45.6 mg/m^3), respectively. The maximum relative deviation for continuous detection(60 min) of 30.4 mg/m^3 H_2S is 0.48% and the minimum detection limit obtained by Allan variance is 79 μg/m^3 with optimal integration time of 32 s. The optical H_2S sensor can be applied to environmental monitoring and industrial production, and it has significance for real-time online detection in many fields.
基金supported by the National Natural Science Foundation of China(Nos.60871073 and 61201075)China Postdoctoral Science Foundation(No.2012M511507)
文摘We discuss the efficiency of an electro-optic (EO) polymer sensor with interdigitated coplanar electrodes. The developed EO sensor is used to detect terahertz radiation via EO sampling. Results show that the sensor improves more significantly detection sensitivity than does a sensor with sandwich configurations.
基金supported by the NNSF of China(Nos.61525402,61604071)the Key University Science Research Project of Jiangsu Province(No.15KJA430006)the Natural Science Foundation of Jiangsu Province(No.BK20161012)
文摘With the rapid development of human artificial intelligence and the inevitably expanding markets, the past two decades have witnessed an urgent demand for the flexible and wearable devices, especially the flexible strain sensors. Flexible strain sensors, incorporated the merits of stretchability, high sensitivity and skin-mountable,are emerging as an extremely charming domain in virtue of their promising applications in artificial intelligent realms, human-machine systems and health-care devices. In this review, we concentrate on the transduction mechanisms, building blocks of flexible physical sensors, subsequently property optimization in terms of device structures and sensing materials in the direction of practical applications. Perspectives on the existing challenges are also highlighted in the end.
文摘Wireless sensors are deployed widely to monitor space, emergent events, and disasters. Collected realtime sensory data are precious for completing rescue missions quickly and efficiently. Detecting isolate safe areas is significant for various applications of event and disaster monitoring since valuable real-time information can be provided for the rescue crew to save persons who are trapped in isolate safe areas. We propose a centralized method to detect isolate safe areas via discovering holes in event areas. In order to shorten the detection delay, a distributed isolate safe area detection method is studied. The distributed method detects isolate safe areas during the process of event detection. Moreover, detecting isolate safe areas in a building is addressed particularly since the regular detecting method is not applicable. Our simulation results show that the distributed method can detect all isolate safe areas in an acceptable short delay.
文摘Compressors play an important role in day-to-day operation in most oil and gas platforms,especially in the case for maintaining gas pressure in transportation pipe.Its complex problem to detect the sensors health and abnormality as the sensor reading would reflect the various states of the compressor.In ideal situation,sensor readings offer vast amounts of information on compressor health and could possibly indicate early fault of machines.Furthermore,due to harsh site and process operating conditions,sensors are often found to have drifted or failed,and there is no standard methodology to predict abnormality apart from applying emerging industrial smart sensor technologies.In this paper,we investigate a minimalist approach for detecting abnormality of compressor's shaft's RPM sensor.As the sensors in the compressor are correlated,we first use the outputs of other sensors to predict the shaft's RPM using regression-based models(neural networks and multiple linear regression).Second,we calculate the histogram of residuals by taking the difference between the predicted sensor value and the actual sensor value plus the abnormality in terms of bias/miscalibration and noise.The histogram of residuals can be used for sensor abnormality monitoring.In general,sensor states can be monitored by observing the shifting of the mean in the histogram of residuals.The sensor readings contaminated with noise can be seen by a shifted mean whose value is between the normal condition mean and the biased condition mean.This method is compact and would be relevant to monitor irregularity of the sensors.
基金Supported by the National Natural Science Foundation of China(20927003,90913013,41273093,21175101)the National Major Scientific Instruments and Device Development Project(2012YQ16000701)the Foundation of China Geological Survey(12120113015200)
文摘A new fluorescence sensor is developed for simultaneous detection of Hg^2+ and Ag^+. During the detection process,Hg^2+ forms complexes with the fluorescent dye rhodamine B isothiocyanate(RBITC) modified onto the surface of gold nanoparticles(Au NPs),resulting in RBITC's displacement from the surface of Au NPs and the recovery of fluorescence. Meanwhile,Ag^+ forms "C-Ag^+-C" complex with C-rich 5-carboxyfluorescein(FAM)-ss DNA modified onto the surface of Au NPs,which keeps the fluorescent dye FAM close to the Au NPs and results in quench of fluorescence. The experimental results show a wide linear range and a good sensitivity. The limit of detection is 1.06 nmol/L for Ag^+ and 0.48 nmol/L for Hg^2+. This detection method is not only easy to operate but also efficient.
基金Project supported by the National Natural Science Foundation of China (No. 29875008).
文摘In this work, the application of a conducting polymer, poly(thionine), modified electrode as matrix to DNA immobilization as well as transducer to label-free DNA hybridization detection was introduced. The electropolymerization of thionine onto electrode surface was carried out by a simple two-step method, which involved a preanodization of glassy carbon electrode at a constant positive potential in thionine solution following cyclic voltammetry scans in the solution. Electrochemical detection was performed by differential pulse voltammetry in the electroactivity potential domain of poly(thionine). The resulting poly(thionine) modified electrode showed a good stability and electroactivity in aqueous media during a near neutral pH range. Additionally, the pendant amino groups on the poly(thionine) chains enabled poly(thionine) modified electrode to immobilize phosphate group terminated DNA probe via covalent linkage. Hybridization process induced a clear decrease in poly(thionine) redox current, which was corresponding to the decrease in poly(thionine) electroactivity after double stranded DNA was formed on the polymer film. The detection limit of this electrochemical DNA hybridization sensor was 1.0 × 10^-10mol/L. Compared with complementary sequence, the hybridization signal values of 1-base mismatched and 3-base mismatched samples were 63.9% and 9.2%, respectively.
基金the National Natural Science Foundation of China(No.51775331)。
文摘In this study,a multi-object tracking(MOT)scheme based on a light detection and ranging sensor was proposed to overcome imprecise velocity observations in object occlusion scenarios.By applying real-time velocity estimation,a modified unscented Kalman filter(UKF)was proposed for the state estimation of a target object.The proposed method can reduce the calculation cost by obviating unscented transformations.Additionally,combined with the advantages of a two-reference-point selection scheme based on a center point and a corner point,a reference point switching approach was introduced to improve tracking accuracy and consistency.The state estimation capability of the proposed UKF was verified by comparing it with the standard UKF in single-target tracking simulations.Moreover,the performance of the proposed MOT system was evaluated using real traffic datasets.