将曼氏无针乌贼幼体置于24 h半致死剂量(24 h LC_(50)=10mg/L)的硫化物溶液中,检测不同时间点曼氏无针乌贼幼体血液生理生化指标的变化。结果表明,随着处理时间的延长,血细胞数量逐步下降(p<0.05);血糖(p>0.05)和总蛋白(p<0.05...将曼氏无针乌贼幼体置于24 h半致死剂量(24 h LC_(50)=10mg/L)的硫化物溶液中,检测不同时间点曼氏无针乌贼幼体血液生理生化指标的变化。结果表明,随着处理时间的延长,血细胞数量逐步下降(p<0.05);血糖(p>0.05)和总蛋白(p<0.05)的含量先降后升;总胆固醇(p>0.05)、甘油三酯浓度(P>0.05)和谷草转氨酶活力(P<0.05)的变化趋势相同,均为先升后降;谷丙转氨酶活力呈"降升降"的变化趋势(p<0.05);而谷草转氨酶和谷丙转氨酶活力变化的比值却呈现"升降升"的变化趋势(p<0.05)。其中血细胞数量降低可能是因为受到了硫化物胁迫及饥饿的双重压力,增加的血糖主要由血脂转化而来。而硫化物胁迫下血液中多项生理生化指标改变,其主要原因是肝细胞受损和免疫力下降,这也是最终造成乌贼幼体大量死亡的重要原因。展开更多
Ten novel polymorphic microsatellite loci were developed using an enrichment and colony hybridization protocol and characterized for the cuttlefish,Sepiella maindroni.Polymorphism was explored by using 30 individuals ...Ten novel polymorphic microsatellite loci were developed using an enrichment and colony hybridization protocol and characterized for the cuttlefish,Sepiella maindroni.Polymorphism was explored by using 30 individuals from the coastal waters of Zhanjiang,Guangdong province,China,in December 2007.The number of alleles ranged from 5 to 13.The ranges of observed and expected heterozygosity were from 0.578 9 to 1.000 0 and 0.682 8 to 0.925 7,respectively,and the average polymorphic information content （P IC） was 0.778 5.These microsatellite loci will certainly facilitate the detection of the genetic variation and population structure of S.maindroni.展开更多
文摘将曼氏无针乌贼幼体置于24 h半致死剂量(24 h LC_(50)=10mg/L)的硫化物溶液中,检测不同时间点曼氏无针乌贼幼体血液生理生化指标的变化。结果表明,随着处理时间的延长,血细胞数量逐步下降(p<0.05);血糖(p>0.05)和总蛋白(p<0.05)的含量先降后升;总胆固醇(p>0.05)、甘油三酯浓度(P>0.05)和谷草转氨酶活力(P<0.05)的变化趋势相同,均为先升后降;谷丙转氨酶活力呈"降升降"的变化趋势(p<0.05);而谷草转氨酶和谷丙转氨酶活力变化的比值却呈现"升降升"的变化趋势(p<0.05)。其中血细胞数量降低可能是因为受到了硫化物胁迫及饥饿的双重压力,增加的血糖主要由血脂转化而来。而硫化物胁迫下血液中多项生理生化指标改变,其主要原因是肝细胞受损和免疫力下降,这也是最终造成乌贼幼体大量死亡的重要原因。
基金The National Key Technology R & D Program of China under contract No.2007BAD43B05the Key Science & Technology Specific Projects of Zhejiang Province under contract No.2007C12062the National Natural Science Foundation of China under contract No.31001109
文摘Ten novel polymorphic microsatellite loci were developed using an enrichment and colony hybridization protocol and characterized for the cuttlefish,Sepiella maindroni.Polymorphism was explored by using 30 individuals from the coastal waters of Zhanjiang,Guangdong province,China,in December 2007.The number of alleles ranged from 5 to 13.The ranges of observed and expected heterozygosity were from 0.578 9 to 1.000 0 and 0.682 8 to 0.925 7,respectively,and the average polymorphic information content （P IC） was 0.778 5.These microsatellite loci will certainly facilitate the detection of the genetic variation and population structure of S.maindroni.
