Objective:To evaluate the value of procalcitonin (PCT) content for adjuvant diagnosis of neonatal septicemia and assessment of its severity.Methods: A total of 100 newborns who were diagnosed with septicemia in the Fi...Objective:To evaluate the value of procalcitonin (PCT) content for adjuvant diagnosis of neonatal septicemia and assessment of its severity.Methods: A total of 100 newborns who were diagnosed with septicemia in the First People's Hospital of Xianyang City between January 2017 and January 2018 were selected as the septicemia group of the research, and the newborns who were born in the First People's Hospital of Xianyang City during the same period, with the general data matched with those of septicemia group and without neonatal diseases after born were selected as the control group. Serum was collected to determine the contents of PCT, inflammatory cytokines and immune cytokines, and peripheral blood was collected to determine the expression intensity of inflammatory signaling molecules and immune transcription factors.Results: Serum PCT, TNF-α, HMGB1, ICAM-1, IL-10 and IL-17 contents as well as peripheral blood TLR4, NF-κB, MPO, RORγt and FOXP3 expression intensity of septicemia group were significantly higher than those of control group whereas peripheral blood HLA-DR expression intensity was lower than that of control group;peripheral blood TLR4, NF-κB, MPO, RORγt and FOXP3 expression intensity as well as serum TNF-α, HMGB1, ICAM-1, IL-10 and IL-17 contents of septicemia group of newborns with high PCT were significantly higher than those of newborns with low PCT whereas peripheral blood HLA-DR expression intensity was lower than that of newborns with low PCT.Conclusion: The increase of PCT in serum of newborns with septicemia is related to the change of inflammatory response and immune response, and its content detection is valuable for assessing the severity of the disease.展开更多
患者,男性,58岁,乙型肝炎后肝硬化失代偿期、2型糖尿病12年,近1个月再发腹胀、水肿,尿少入院。住院期间患者寒战、发热,取血标本进行培养,选用头孢哌酮/舒巴坦经验性抗感染治疗。确诊非O1/O139霍乱弧菌引起的血流感染,药敏试验提示头孢...患者,男性,58岁,乙型肝炎后肝硬化失代偿期、2型糖尿病12年,近1个月再发腹胀、水肿,尿少入院。住院期间患者寒战、发热,取血标本进行培养,选用头孢哌酮/舒巴坦经验性抗感染治疗。确诊非O1/O139霍乱弧菌引起的血流感染,药敏试验提示头孢哌酮/舒巴坦敏感后,继续使用当前药物,头孢哌酮/舒巴坦由3 g q12h改为3 g q8h治疗。10 d后患者无发热,血培养和粪便霍乱弧菌培养阴性,好转出院。展开更多
DEAR EDITOR, Hemorrhagic septicemia is an acute, highly fatal disease that affects goldfish (Carassius auratus). To gain a better understanding of related immune genes, the transcriptomes of the skin and head kidney o...DEAR EDITOR, Hemorrhagic septicemia is an acute, highly fatal disease that affects goldfish (Carassius auratus). To gain a better understanding of related immune genes, the transcriptomes of the skin and head kidney of goldfish suffering hemorrhagic septicemia were sequeneed, assembled, and characterized.展开更多
Objective:To explore a rapid diagnostic method in neonatal sepsis and bacterial meningitis. Methods:The primers were designed and synthesized hased on 16S rRNA gene of Staphylococcus aureus.Four specimens of Staphyloc...Objective:To explore a rapid diagnostic method in neonatal sepsis and bacterial meningitis. Methods:The primers were designed and synthesized hased on 16S rRNA gene of Staphylococcus aureus.Four specimens of Staphylococcus aureus.16 specimens of coagulasenegative Staphylococci.2 specimens of Enterococci.3 specimens of Streptococcus,1 specimen of Micrococcus.3 specimens of Escherichia coli.4 specimens of Klebsiella pneumoniae,3 specimens of Pseudomonas aeruginosa,2 specimens of Enlerohacter cloacae.and 5 specimens of Acinetobacter were tested by loop-mediated isothermal amplification(LAMP) assay.A total of118 clinical specimens of sepsis and non-sepsis were collected and detected with both LAMP assay and blood culture.Results:Bv designing primers specific for Staphylococcus aureus,specimens containing different kinds of pathogens were earned out by LAMP assay,and our data showed LAMP technology lor the specific detection of Staphylococcus aureus in samples was successlully established.All clinical Specimens of sepsis and non-sepsis were tested by both blood cultures and LAMP,and our data showed that compared wit blood culture method,the LAMP technology, showed significantly high detection rale(P 【0.01).Conclusions:As a quick and easy detection of Staphylococcus aureus,the LAMP technology was successfully established,laid the foundation for the diagnosis and treatment of children Staphylococcus aureus sepsis,and showed great promotion and application value.展开更多
OBJECTIVE Xingnaojing injection(XNJ) is an extracts of Angong Niuhuang Pill that is a well-known traditional Chinese medicine used for the treatment of septicemia and stroke.This study aims to investigate the effect o...OBJECTIVE Xingnaojing injection(XNJ) is an extracts of Angong Niuhuang Pill that is a well-known traditional Chinese medicine used for the treatment of septicemia and stroke.This study aims to investigate the effect of XNJ on intestinal mucosal barrier in septicemia and intracerebral hem.orrhage(ICH) mice models.METHODS The septicemia mice models were induced by intravenous in.jection with lipopolysaccharide(20 mg·kg^(-1)).And the ICH mice models were made by intrastriatal injec.tion of bacterial collagenase.The septicemia animals were treated intravenously with XNJ at dose of2.5,5,10,or 15 mL·kg^(-1).The ICH animals were treated intravenously with XNJ at dose of 10 mL·kg^(-1).Thereafter,the permeability of intestinal mucosa was assayed by FITC-D method.RESULTS Com.pared with the control group(44.72±4.30),the permeability of intestinal mucosa in the mice in septice.mia group(233.68±28.18) was significantly increased(P<0.01).Treatment with XNJ at dose of 5,10,and 15 mL·kg^(-1) reduced the permeability of intestinal mucosa(150.45±17.52,139.21±17.05,132.55±18.88,respectively,P<0.01) except 2.5 mL·kg^(-1)(240.71±21.42,P>0.01);Compared with sham group(57.88±7.31),the permeability of intestinal mucosa in the mice of ICH(282.25±23.78) was significantly in.creased(P<0.01).Treatment with XNJ(10 mL·kg^(-1)) in the mice of ICH group ameliorated the change of permeability in intestinal mucosa(148.83±15.86,P<0.01).CONCLUSION XNJ exhibits the protec.tive effect on the intestinal mucosal barrier in septicemia and ICH,which will prevent the endotoxin to penetrate the intestinal mucosa and then to enter the circulation in infections and stress.展开更多
A previously healthy 53-year-old woman was urgently hospitalized due to septic shock. She was diagnosed with bacterial pneumonia based on chest radiograph and computed tomography findings of right upper lobe consolida...A previously healthy 53-year-old woman was urgently hospitalized due to septic shock. She was diagnosed with bacterial pneumonia based on chest radiograph and computed tomography findings of right upper lobe consolidation. Sputum Gramstain at the time of admission showed gram-negative rods with phagocytosis. Intravenous meropenem was immediately initiated as empiric antibacterial therapy. Bacterial culture specimens from sputum and blood were positive for Pseudomonas aeruginosa. Following appropriate antibiotic therapies, the patient recovered from a shock state and gradually became well. There has been no evidence of recurrence at 6 months after discharge. P. aeruginosa community acquired pneumonia with septicemia is rapidly progressive and often fatal. The choice of initial empiric antibiotic treatment that is active against P. aeruginosa is critical in improving outcome.展开更多
In this paper,a sliding mode observer scheme of sensor fault diagnosis is proposed for a class of time delay nonlinear systems with input uncertainty based on neural network.The sensor fault and the system input uncer...In this paper,a sliding mode observer scheme of sensor fault diagnosis is proposed for a class of time delay nonlinear systems with input uncertainty based on neural network.The sensor fault and the system input uncertainty are assumed to be unknown but bounded.The radial basis function (RBF) neural network is used to approximate the sensor fault.Based on the output of the RBF neural network,the sliding mode observer is presented.Using the Lyapunov method,a criterion for stability is given in terms of matrix inequality.Finally,an example is given for illustrating the availability of the fault diagnosis based on the proposed sliding mode observer.展开更多
文摘Objective:To evaluate the value of procalcitonin (PCT) content for adjuvant diagnosis of neonatal septicemia and assessment of its severity.Methods: A total of 100 newborns who were diagnosed with septicemia in the First People's Hospital of Xianyang City between January 2017 and January 2018 were selected as the septicemia group of the research, and the newborns who were born in the First People's Hospital of Xianyang City during the same period, with the general data matched with those of septicemia group and without neonatal diseases after born were selected as the control group. Serum was collected to determine the contents of PCT, inflammatory cytokines and immune cytokines, and peripheral blood was collected to determine the expression intensity of inflammatory signaling molecules and immune transcription factors.Results: Serum PCT, TNF-α, HMGB1, ICAM-1, IL-10 and IL-17 contents as well as peripheral blood TLR4, NF-κB, MPO, RORγt and FOXP3 expression intensity of septicemia group were significantly higher than those of control group whereas peripheral blood HLA-DR expression intensity was lower than that of control group;peripheral blood TLR4, NF-κB, MPO, RORγt and FOXP3 expression intensity as well as serum TNF-α, HMGB1, ICAM-1, IL-10 and IL-17 contents of septicemia group of newborns with high PCT were significantly higher than those of newborns with low PCT whereas peripheral blood HLA-DR expression intensity was lower than that of newborns with low PCT.Conclusion: The increase of PCT in serum of newborns with septicemia is related to the change of inflammatory response and immune response, and its content detection is valuable for assessing the severity of the disease.
文摘患者,男性,58岁,乙型肝炎后肝硬化失代偿期、2型糖尿病12年,近1个月再发腹胀、水肿,尿少入院。住院期间患者寒战、发热,取血标本进行培养,选用头孢哌酮/舒巴坦经验性抗感染治疗。确诊非O1/O139霍乱弧菌引起的血流感染,药敏试验提示头孢哌酮/舒巴坦敏感后,继续使用当前药物,头孢哌酮/舒巴坦由3 g q12h改为3 g q8h治疗。10 d后患者无发热,血培养和粪便霍乱弧菌培养阴性,好转出院。
基金supported by a grant from the Key Project of Scientific and Technological Innovation of Blue Granary(2018YFD0901505)
文摘DEAR EDITOR, Hemorrhagic septicemia is an acute, highly fatal disease that affects goldfish (Carassius auratus). To gain a better understanding of related immune genes, the transcriptomes of the skin and head kidney of goldfish suffering hemorrhagic septicemia were sequeneed, assembled, and characterized.
文摘Objective:To explore a rapid diagnostic method in neonatal sepsis and bacterial meningitis. Methods:The primers were designed and synthesized hased on 16S rRNA gene of Staphylococcus aureus.Four specimens of Staphylococcus aureus.16 specimens of coagulasenegative Staphylococci.2 specimens of Enterococci.3 specimens of Streptococcus,1 specimen of Micrococcus.3 specimens of Escherichia coli.4 specimens of Klebsiella pneumoniae,3 specimens of Pseudomonas aeruginosa,2 specimens of Enlerohacter cloacae.and 5 specimens of Acinetobacter were tested by loop-mediated isothermal amplification(LAMP) assay.A total of118 clinical specimens of sepsis and non-sepsis were collected and detected with both LAMP assay and blood culture.Results:Bv designing primers specific for Staphylococcus aureus,specimens containing different kinds of pathogens were earned out by LAMP assay,and our data showed LAMP technology lor the specific detection of Staphylococcus aureus in samples was successlully established.All clinical Specimens of sepsis and non-sepsis were tested by both blood cultures and LAMP,and our data showed that compared wit blood culture method,the LAMP technology, showed significantly high detection rale(P 【0.01).Conclusions:As a quick and easy detection of Staphylococcus aureus,the LAMP technology was successfully established,laid the foundation for the diagnosis and treatment of children Staphylococcus aureus sepsis,and showed great promotion and application value.
文摘OBJECTIVE Xingnaojing injection(XNJ) is an extracts of Angong Niuhuang Pill that is a well-known traditional Chinese medicine used for the treatment of septicemia and stroke.This study aims to investigate the effect of XNJ on intestinal mucosal barrier in septicemia and intracerebral hem.orrhage(ICH) mice models.METHODS The septicemia mice models were induced by intravenous in.jection with lipopolysaccharide(20 mg·kg^(-1)).And the ICH mice models were made by intrastriatal injec.tion of bacterial collagenase.The septicemia animals were treated intravenously with XNJ at dose of2.5,5,10,or 15 mL·kg^(-1).The ICH animals were treated intravenously with XNJ at dose of 10 mL·kg^(-1).Thereafter,the permeability of intestinal mucosa was assayed by FITC-D method.RESULTS Com.pared with the control group(44.72±4.30),the permeability of intestinal mucosa in the mice in septice.mia group(233.68±28.18) was significantly increased(P<0.01).Treatment with XNJ at dose of 5,10,and 15 mL·kg^(-1) reduced the permeability of intestinal mucosa(150.45±17.52,139.21±17.05,132.55±18.88,respectively,P<0.01) except 2.5 mL·kg^(-1)(240.71±21.42,P>0.01);Compared with sham group(57.88±7.31),the permeability of intestinal mucosa in the mice of ICH(282.25±23.78) was significantly in.creased(P<0.01).Treatment with XNJ(10 mL·kg^(-1)) in the mice of ICH group ameliorated the change of permeability in intestinal mucosa(148.83±15.86,P<0.01).CONCLUSION XNJ exhibits the protec.tive effect on the intestinal mucosal barrier in septicemia and ICH,which will prevent the endotoxin to penetrate the intestinal mucosa and then to enter the circulation in infections and stress.
文摘A previously healthy 53-year-old woman was urgently hospitalized due to septic shock. She was diagnosed with bacterial pneumonia based on chest radiograph and computed tomography findings of right upper lobe consolidation. Sputum Gramstain at the time of admission showed gram-negative rods with phagocytosis. Intravenous meropenem was immediately initiated as empiric antibacterial therapy. Bacterial culture specimens from sputum and blood were positive for Pseudomonas aeruginosa. Following appropriate antibiotic therapies, the patient recovered from a shock state and gradually became well. There has been no evidence of recurrence at 6 months after discharge. P. aeruginosa community acquired pneumonia with septicemia is rapidly progressive and often fatal. The choice of initial empiric antibiotic treatment that is active against P. aeruginosa is critical in improving outcome.
基金Natural Science Foundation of Jiangsu Province (No.SBK20082815)Aeronautical Science Foundation of China (No.20075152014)
文摘In this paper,a sliding mode observer scheme of sensor fault diagnosis is proposed for a class of time delay nonlinear systems with input uncertainty based on neural network.The sensor fault and the system input uncertainty are assumed to be unknown but bounded.The radial basis function (RBF) neural network is used to approximate the sensor fault.Based on the output of the RBF neural network,the sliding mode observer is presented.Using the Lyapunov method,a criterion for stability is given in terms of matrix inequality.Finally,an example is given for illustrating the availability of the fault diagnosis based on the proposed sliding mode observer.
