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Sequence Analysis of Mitochondrial DNA D-loop Region in Xinjiang Goose 被引量:1
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作者 邵勇钢 岳涛 +1 位作者 李建华 刘银凤 《Agricultural Science & Technology》 CAS 2012年第11期2290-2292,2337,共4页
[Objective] The sequences of mitochondrial DNA D-loop region of Xinjiang Goose with three different colors of plumage were analyzed in order to study the genetic diversity of Xinjiang Goose, as well as the phylogeny a... [Objective] The sequences of mitochondrial DNA D-loop region of Xinjiang Goose with three different colors of plumage were analyzed in order to study the genetic diversity of Xinjiang Goose, as well as the phylogeny and evolution. [Method] Ten geese were selected randomly from the core populations of grey-, mosaic- and white-plumaged Xinjiang Goose respectively with a total number of thirty as experi- mental materials, of which the blood samples were collected from the largest vein under the wing (brachial vein) for DNA extraction. Sequences of mitochondrial DNA D-loop regions were determined using DNA sequencing technology to analyze the polymorphism. In addition, the genetic distances among different populations were estimated through the comparison with the reference sequences. [Resull] The con- tents of A, G, C and T nucleotides in the D-loop region of Xinjiang Goose were 28.85%, 17.05%, 25.38% and 28.72%, respectively. The average haplotype diversity and nucleotide diversity of Xinjiang Goose were 0.583 and 0.056. Xinjiang Goose and Greylag Goose were clustered into the same group. [Conclusion] The results showed that Xinjiang Geese with three different colors of plumage all descend from Greylag Goose (Anser anser). 展开更多
关键词 Xinjiang Goose Mitochondrial dna D-loop region sequence analysis
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Study on the Detection of Telomerase Activity by Combining DNA Sequence Analysis with TRAP
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作者 金礼吉 《High Technology Letters》 EI CAS 2001年第3期8-10,共3页
Telomeric repeat amplification protocol (TRAP) is now a conventional assay for detecting telomerase activity. However, this method presents problems owing to tedious quantitation, radioisotopic handling. In order to a... Telomeric repeat amplification protocol (TRAP) is now a conventional assay for detecting telomerase activity. However, this method presents problems owing to tedious quantitation, radioisotopic handling. In order to alleviate these inconveniences, a novel telomerase DNA sequencing assay together with TRAP to detect human telomerase activity was developed. It was used to detect telomerase activity in Hela, HLF, MCF, K562, SMMC 7721 cells, Leukocytes and RNase pretreated or heat treated cells as control. Telomerase activity assayed by this method was positive when the number of K562 cells examined was 102,103, and 104. The telomerase activity depended on the number of K562 cells used in the assay. Telomerase activity of Rnase pretreated cells or heat treated cells, and human normal peripheral blood leukocyte(Leu) were negative. The result of this method was available within a few hours and was handled without radioisotope. Further studies should be taken to detect telomerase activity in quantitation. 展开更多
关键词 TELOMERASE ACTIVITY TRAP dna sequence analysis
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Isolation of a Gastrodia Antifungal Protein Gene from a Genomic Library of G. elata and Its Sequence Analysis
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作者 萨其拉 Wang +6 位作者 Yiqin Li Wenbin Zhang Liming Sun Yongru 《High Technology Letters》 EI CAS 2002年第3期36-39,共4页
A new genomic DNA encoding a member of Gastrodia antifungal protein family is isolated and sequenced. This gene contains a 510 bp open reading frame and 531 bp promoter region without introns. Sequence analysis indica... A new genomic DNA encoding a member of Gastrodia antifungal protein family is isolated and sequenced. This gene contains a 510 bp open reading frame and 531 bp promoter region without introns. Sequence analysis indicates that a 28 amino acids signal peptide exists at the N terminal. It shows high sequence homology with the mannose binding lectins from Epipactis helleborine, Listera ovata and Cymbidium hybrid. A putative TATA box and transcription start site is detected in the promoter region. 展开更多
关键词 Gastrodia elata genomic dna sequence analysis
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Detection of rare mutation of β-thalassemia by direct sequence analysis of the PCR products
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作者 单越新 张基增 徐钤 《Journal of Medical Colleges of PLA(China)》 CAS 1993年第3期235-241,共7页
A technique of direct sequence analysis of β-globin gene with the products of amplifi-cation by polymerase chain reaction (PCR) was reported and a case of β-thalassemia with therare mutation in Chinese,‘codon 14/15... A technique of direct sequence analysis of β-globin gene with the products of amplifi-cation by polymerase chain reaction (PCR) was reported and a case of β-thalassemia with therare mutation in Chinese,‘codon 14/15 (+G)’ was detected by this method.After the se-quence of the mutation site was determined,an analysis of the restriction map of the gene anddot blot hybridization with radioactive allele specific oligonucleotide probe was designed to con-firm the result of DNA sequencing. 展开更多
关键词 POLYMERASE CHAIN reaction(PCR) MUTATION dna sequence analysis Β-THALASSEMIA
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Sequence analysis of lacZ^- mutations induced by ion beam irradiation in double-stranded M13mp18DNA 被引量:7
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作者 杨剑波 吴李君 +3 位作者 李莉 吴家道 余增亮 许智宏 《Science China(Life Sciences)》 SCIE CAS 1997年第1期107-112,共6页
While M13mpl8 double-stranded DNA was irradiated with ion beam, and transfected into E. coli JM103, a decrease of transfecting activity was discovered. The lacZ-mutation frequency at 20% survival could reach (3.6-16.8... While M13mpl8 double-stranded DNA was irradiated with ion beam, and transfected into E. coli JM103, a decrease of transfecting activity was discovered. The lacZ-mutation frequency at 20% survival could reach (3.6-16.8) × 104, about 2.3-10 times that of unirradiated M13DNA. Altogether, 27 lacZ~ mutants were select-ed, 10 of which were used for sequencing. 7 of the sequenced mutants show base changes in 250-bp region examined (the remaining 3 mutants probably have base changes outside the regions sequenced). 5 of the base-changed mutants contain more than one mutational base sites (some of them even have 5-6 mutational base sites in 250-bp region ex-amined) ; this dense distribution of base changes in polysites has seldom been seen in X-rays, γ-rays or UV induced DNA mutations. Our experiments also showed that the types of base changes include transitions( 50 % ), transversions (45% ) and deletion (5% ); no addition or duplication was observed. The transitions were mainly C→T and A→G; the transversions were mainly C→A and C→G. The mutations involving cytosine residue (in the template strand) con-stitute about 60% of all the base changes observed. In comparison with the surrounding sequences of mutational base sites, the base located between TG and CT is found to be easily substituted. 展开更多
关键词 ion beam IRRADIATION M13dna lacZ^- MUTATION sequence analysis.
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Determining the utility of standard hospital microbiology testing: Comparing standard microbiology cultures with DNA sequence analysis in patients with chronic sinusitis 被引量:1
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作者 Sarah K.Rapoport Alyssa J.Smith +3 位作者 Maxwell Bergman Kelly A.Scriven Itzhak Brook Suzette K.Mikula 《World Journal of Otorhinolaryngology-Head and Neck Surgery》 2019年第2期82-87,共6页
Objective:To demonstrate DNA sequencing analysis (DNAsa) of sinus cultures in patients with CRS is a reliable method of detecting pathogens in polymicrobial CRS infections.Methods:After obtaining Institutional Review ... Objective:To demonstrate DNA sequencing analysis (DNAsa) of sinus cultures in patients with CRS is a reliable method of detecting pathogens in polymicrobial CRS infections.Methods:After obtaining Institutional Review Board approval for this prospective cohort study,we selected a random sample of 50 patients with CRS at Medstar Georgetown University Hospital between September 2016 and March 2017.We defined CRS as a history of rhinosinusitis refractory to maximal medical therapy and prior endoscopic sinus surgery.Patients demonstrating active purulence in a sinus cavity were prospectively selected to undergo standard hospital cultures (SHC) and DNAsa cultures.Organisms identified in both methods were compared for each patient.Results:Specimens were obtained from 29 female and 16 male patients with a mean age of 50 years.A total of 45 cultures were included in our final analysis;five cultures were excluded after inappropriate laboratory processing.Results from these patients were compared and analyzed.Cohen's weighted kappa analysis showed agreement between the two testing methods in identifying predominant microorganisms.DNAsa detected 31.9% more microorganisms compared to SHC (P < 0.05).When multiple microorganisms were detected,DNAsa yielded more positive results compared to SHC (P < 0.05).Conclusions:DNAsa detects all microorganisms identified by SHC as well as predominant microorganisms not detected by SHC.Thus molecular pathogen identification may be more reliable for identifying multiple microorganisms as compared to standard culture techniques that identify only one or two microorganisms.In recalcitrant cases of CRS,DNAsa may provide better guidance in selection of appropriate antimicrobial treatment. 展开更多
关键词 Chronic RHINOSINUSITIS (CRS) dna sequencING analysis (dnasa) MICROBIOME Molecular sequencING MICROBIOLOGY culture
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Analysis method and algorithm design of biological sequence problem based on generalized k-mer vector
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作者 LIU Wen-li WU Qing-biao 《Applied Mathematics(A Journal of Chinese Universities)》 SCIE CSCD 2021年第1期114-127,共14页
K-mer can be used for the description of biological sequences and k-mer distribution is a tool for solving sequences analysis problems in bioinformatics.We can use k-mer vector as a representation method of the k-mer ... K-mer can be used for the description of biological sequences and k-mer distribution is a tool for solving sequences analysis problems in bioinformatics.We can use k-mer vector as a representation method of the k-mer distribution of the biological sequence.Problems,such as similarity calculations or sequence assembly,can be described in the k-mer vector space.It helps us to identify new features of an old sequence-based problem in bioinformatics and develop new algorithms using the concepts and methods from linear space theory.In this study,we defined the k-mer vector space for the generalized biological sequences.The meaning of corresponding vector operations is explained in the biological context.We presented the vector/matrix form of several widely seen sequence-based problems,including read quantification,sequence assembly,and pattern detection problem.Its advantages and disadvantages are discussed.Also,we implement a tool for the sequence assembly problem based on the concepts of k-mer vector methods.It shows the practicability and convenience of this algorithm design strategy. 展开更多
关键词 vector space biological sequence k-mer algorithm design analysis method.
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A New Inter-Sequence Analysis Method Using Objective Scaling Measure
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作者 LI Lingyu LI Weiwei +1 位作者 YI Pingtao GUO Yajun 《Wuhan University Journal of Natural Sciences》 CAS CSCD 2020年第1期27-32,共6页
The inter-sequence analysis method is a popular decision making method considering subjective information of the decision maker.The validity of this method is sensitive to the subjective information provided by the de... The inter-sequence analysis method is a popular decision making method considering subjective information of the decision maker.The validity of this method is sensitive to the subjective information provided by the decision maker significantly.Thus,a new weighting method based on the inter-sequence analysis method is proposed.This new method can appropriately reduce the participation of the subjective judgment of the decision maker,and then accordingly decrease the influence of the subjective judgment to the results.It only needs the decision maker to give the inter sequence of the criteria.The importance comparison of two criteria is objectively determined by considering the ratio of a criterion being superior to another one.Some numerical examples are given to illustrate the calculation process of the new method. 展开更多
关键词 MULTI-CRITERIA decision making inter-sequence analysis method weighting method SUPERIORITY number transition function
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Discriminant Classification Model of DNA Sequence 被引量:1
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作者 王显金 阳军 《Agricultural Science & Technology》 CAS 2011年第6期781-784,共4页
[Objective] The research aimed to construct the discriminant classification model of DNA sequence by combining with the biology knowledge and the mathematical method.[Method] According to the polarity nature of side c... [Objective] The research aimed to construct the discriminant classification model of DNA sequence by combining with the biology knowledge and the mathematical method.[Method] According to the polarity nature of side chain radical in the amino acid,the classification information of amino acid which represented the sequence characteristic from the content and array situation of base was extracted from the different sequences that the amino acid content was different.The four-dimension vector was used to represent.Mahalanobis distance and Fisher discriminant methods were used to classify the given sequence.[Result] In the model,the back substitution rates of sample obtained by two kinds of classification methods were both 100%,and the consistent rate of classification was 90%.[Conclusion] In the model,the calculation method was simple,and the accuracy of classification result was higher.It was superior to the discriminant classification model which was only based on the base content. 展开更多
关键词 dna sequence CODON Discriminant analysis FREQUENCY
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Complete Sequence of Proviral DNA of Equine Infectious Anemia Virus Strain L
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作者 LIU Hong-quan, WANG Liu, YANG Zhi-biao, KONG Xian-gang and TONG Guang-Zhi(National Key Labortory of Veterinary Biotechnology, Harbin Veterinary Research Institute , CAAS , Harbin 150001) 《Agricultural Sciences in China》 CAS CSCD 2002年第2期232-237,共6页
Equine infectious anemia virus strain L (EIAV-L) is the parental virulent virus of equine infectious anemia donkey leukocyte attenuated vaccine (DLA EIAV). In this study, peripheral blood leukocytes(PBL) were collecte... Equine infectious anemia virus strain L (EIAV-L) is the parental virulent virus of equine infectious anemia donkey leukocyte attenuated vaccine (DLA EIAV). In this study, peripheral blood leukocytes(PBL) were collected from a horse infected with EIAV-L.The PBL DNAs were extracted.The EIAV-L proviral DNA was amplified in four parts covering the entire proviral genomic sequence by polymerase chain reaction (PCR). Each of the four parts was cloned into the plasmid pBluescript SK, and the recombinant plasmids were designated as p2.8, p2.4. p3.1, and p1.2 respectively. After identification with restriction digestion, the inserts within the four plasmids were sequenced. The complete nucleotide sequence of EIAV-L provirus was determined by analyzing each of the four parts and connecting them as a whole. The genome of EIAV-L is 8235 bp in length, and G + C content is 38%. The comparison analysis by the computer software DNASIS showed that the sequence of EIAV-L shares 98.4% and 96.9% identities with that of D-A EIAV and DLA EIAV respectively. The high homology between these strains showed that they were genetically related. The homology between EIAV-L and D-A EIAV is higher than that between EIAV-L and DLA EIAV, and this is consistent with the derivation progress of DLA EIAV. At both ends of EIAV-L provirus, there is an identical long terminal repeat (LTR) sequence of 316bp in length. The LTR consists of U3, R, and U5 regions. The genome of EIAV-L provirus has three long open reading frames(ORF) corresponding to gag, pol and env genes respectively. The gag gene is 1200bp and located at position 613-1912nt. The pol gene is 3402bp and located at position 1708-5109nt. There is a termination codon within the env dividing it into two parts, envl of 699bp (position 5305-6003nt)and env2 of 1827bp (position 6073-7899nt). The provirus has three additional small ORFs: S1, S2 and S3 with sizes of 153bp(position 5113-5265nt), 204bp(position 5279-5482nt)and 402bp(position 7245-7646nt) respectively. 展开更多
关键词 Equine infectious anemia virus Strain L Proviral dna sequence analysis
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A NOVEL HUMAN DNA SEQUENCE WITH TUMOR METASTASIS SUPPRESSIVE ACTIVITY
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作者 葛学铭 陆应麟 +2 位作者 付生法 范文红 刘爽 《Chinese Journal of Cancer Research》 SCIE CAS CSCD 2000年第2期91-95,共5页
Objective: To isolate human tumor metastasis suppressive DNA sequence and to study the molecular mechanisms regulating tumor metastasis. Methods: A mouse lung adenocarcinoma cell clone 12 derived from its parent cell ... Objective: To isolate human tumor metastasis suppressive DNA sequence and to study the molecular mechanisms regulating tumor metastasis. Methods: A mouse lung adenocarcinoma cell clone 12 derived from its parent cell line LM2, which had been transduced with normal human genomic DNA, was previously reported. Compared with LM2, the metastatic potential of clone 12 was very much decreased. Clone 12 was used in this study to amplify the human DNA fragments by Inter Alu PCR technique. The human DNA fragments obtained were then transfected into LM2 cells and their malignant phenotype was tested in vitro and in vivo, and compared with that of the untransfected LM2 cells.Results Three human DNA fragments of 700, 500 and 300 bp were isolated. DNA sequencing revealed that the 700bp fragment does not show homology with hitherto reported genes and was accepted by the Genbank (pt712 U67835). In vitro proliferation and colony formation in soft agar of the 700 bp fragment-transfected LM2 cells were significantly inhibited as compared to the untransfected LM2 cells. Upon subcutaneous inoculation to syngeneic T739 mice, the 700bp-transfected LM2 cells grew more slowly and smaller tumors developed compared to the untransfected ones. Moreover, lung metastasis was not found in 6 of 10 mice inoculated with the 700bp-transfected LM2 cells, while it was found in 9 of 10 mice inoculated with the untransfected LM2 cells. The difference was statistically significant (P<0.001). The frequency of lymph node metastasis was also statistically different between the 2 groups of mice.Conclusion The newly isolated 700bp human DNA fragment may be a metastasis suppressor gene of malignant tumor. 展开更多
关键词 Neoplasm metastatic suppressor gene Human genomic dna Gene transfection sequence analysis Inter Alu PCR
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Isolation and Purification of Carp Mitochondrial DNA and Structural Analysis of Its tRNA^(Cys) Gene and the Light Strand Origin
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作者 吴乃虎 周立伟 +2 位作者 王钢峰 阎景智 冯羽 《Developmental and Reproductive Biology》 1993年第2期1-9,共9页
A new method is presented with which we isolated milochondrial DNA from fresh carp liver usingdifferential centrifugation and DNase treatment that gave high yield of purified product with an easyand economical procedu... A new method is presented with which we isolated milochondrial DNA from fresh carp liver usingdifferential centrifugation and DNase treatment that gave high yield of purified product with an easyand economical procedure. Highly distinct bands were displayed in agarose gel electrophoresls ofthe product digested with restrictlon enzymes, which were successfully used in constructingrestriction map and molecular clone of mitochondrial genes. With DNAs thus obtained, we havecloned cysteine tRNA gene (tRNA^(Cys) gene) of carp mitochondria, determined the nucleotide sequenceof it and the light strand origin, and depicted the cloverleaf secondary structure of tDNA^(Cya) and thelight strand origin. Analysis of nucleotide sequences of tRNA^(Cy) genes of 5 vertebrates has revealedunusual features of carp mitochondrial tRNA^(Cy) gene as compared with their cytoplasmic counter-parts, Altogether 36 bases were found in the light strand origin of carp mitochondriaf: 11 pairs in thestem; and 14 bases in the loop. As compared with those of other 11 vertebrate species, the sequenceof the stem is very conservative while both sequence and length of the loop are quite variable. Thestructure of the stem-loop may play an important role in light strand replication. 展开更多
关键词 Mitochondrial dna (mtdna) tRNA^(Cys) Gene Light Strand Origin sequence analysis
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Comparative analysis of dideoxy sequencing,the KRAS StripAssay and pyrosequencing for detection of KRAS mutation 被引量:8
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作者 Jing Gao Yan-Yan Li +1 位作者 Ping-Nai Sun Lin Shen 《World Journal of Gastroenterology》 SCIE CAS CSCD 2010年第38期4858-4864,共7页
AIM:To compare the differences between dideoxy sequencing/KRAS StripAssay/pyrosequencing for detection of KRAS mutation in Chinese colorectal cancer (CRC) patients.METHODS:Formalin-f ixed, paraff in-embedded (FFPE) sa... AIM:To compare the differences between dideoxy sequencing/KRAS StripAssay/pyrosequencing for detection of KRAS mutation in Chinese colorectal cancer (CRC) patients.METHODS:Formalin-f ixed, paraff in-embedded (FFPE) samples with tumor cells ≥ 50% were collected from 100 Chinese CRC patients at Beijing Cancer Hospital. After the extraction of genome DNA from FFPE samples, fragments contained codons 12 and 13 of KRAS exon 2 were amplified by polymerase chain reaction and analyzed by dideoxy sequencing, the KRAS Strip Assay and pyrosequencing. In addition, the sensitivities of the 3 methods were compared on serial dilutions (contents of mutant DNA: 100%,50%,20%, 5%,10%, 5%,1%,0%) of A549 cell line DNA (carrying the codon 12 Gly>Ser mutation) into wild-type DNA (human normal intestinal mucosa). The results of dideoxy sequencing,the KRAS StripAssay and pyrosequencing were analyzed by Chromas Software, Collector forKRAS Strip Assay and the pyrosequencing PyroMarkTM Q24 system, respectively.RESULTS: Among 100 patients, KRAS mutations were identif ied in 34%, 37% and 37% of patients by dideoxy sequencing, the KRAS StripAssay and pyrosequencing, respectively. The sensitivity was highest with the KRAS Strip Assay (1%), followed by pyrosequencing (5%), and dideoxy sequencing was lowest (15%). Six different mutation types were found in this study with 3 main mutations Gly12 Asp (GGT>GAT), Gly12 Val (GGT>GTT) and Gly13 Asp (GGC>GAC). Thirty-three patients were identifi ed to have KRAS mutations by the 3 methods, and a total of 8 patients had conflicting results between 3 methods: 4 mutations not detected by dideoxy sequencing and the KRAS StripAssay were identified by pyrosequencing; 3 mutations not detected by dideoxy sequencing and pyrosequencing were identif ied by the KRAS StripAssay; and 1 mutation not detected by pyrosequencing was conf irmed by dideoxy sequencing and the KRAS StripAssay. Among these discordant results, the results identif ied by dideoxy sequencing were consistent either with the KRAS StripAssay or with pyrosequencing, which indicated that the accuracy of dideoxy sequencing was high. CONCLUSION: Taking a worldwide view of reports and our results,dideoxy sequencing remains the most popular method because of its low cost and high accuracy. 展开更多
关键词 dna mutational analysis KRAS MUTATION Dideoxy sequencing KRAS StripAssay PYROsequencING
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Analysis of Mitochondrial DNA from the Ancient Tombs of Turfan 被引量:3
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作者 CUI Yin-qiu DUAN Ran-hui +5 位作者 LIU Shu-bai JI Chao-neng ZHU Hong LI Wei MAO Yu-min ZHOU Hui 《Chemical Research in Chinese Universities》 SCIE CAS CSCD 2002年第4期419-423,共5页
MtDNA was successfully extracted from ten individual bones (femurs) in the tombs of ancient Jushi in Turfan basin, dated back to the year about 3 000-2 500 years ago. By means of four overlapping primers, we got nucl... MtDNA was successfully extracted from ten individual bones (femurs) in the tombs of ancient Jushi in Turfan basin, dated back to the year about 3 000-2 500 years ago. By means of four overlapping primers, we got nucleotide sequence of the 218bp length. Ancient mtDNA was analyzed by the sequencing of hypervariable region Ⅰ of the mtDNA control region. The result shows that 9 haplotypes with 24 polymorphic sites were obtained. The phylogenetic analysis indicated that Mongolians and Altai are the population genetically closest to the Jushi groups and Jushi mtDNA pool being an admixture of eastern Asian and European lineages. So our preliminary data imply that an ancient mingling of Euro-Asian population had existed in Turfan basin prior to the early Iron Age. 展开更多
关键词 Ancient dna sequence Phylogenetic analysis
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Variant Map System to Simulate Complex Properties of DNA Interactions Using Binary Sequences 被引量:1
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作者 Jeffrey Zheng Weiqiong Zhang +2 位作者 Jin Luo Wei Zhou Ruoyu Shen 《Advances in Pure Mathematics》 2013年第7期5-24,共20页
Stream cipher, DNA cryptography and DNA analysis are the most important R&D fields in both Cryptography and Bioinformatics. HC-256 is an emerged scheme as the new generation of stream ciphers for advanced network ... Stream cipher, DNA cryptography and DNA analysis are the most important R&D fields in both Cryptography and Bioinformatics. HC-256 is an emerged scheme as the new generation of stream ciphers for advanced network security. From a random sequencing viewpoint, both sequences of HC-256 and real DNA data may have intrinsic pseudo-random properties respectively. In a recent decade, many DNA sequencing projects are developed on cells, plants and animals over the world into huge DNA databases. Researchers notice that mammalian genomes encode thousands of large noncoding RNAs (lncRNAs), interact with chromatin regulatory complexes, and are thought to play a role in localizing these complexes to target loci across the genome. It is a challenge target using higher dimensional visualization tools to organize various complex interactive properties as visual maps. The Variant Map System (VMS) as an emerging scheme is systematically proposed in this paper to apply multiple maps that used four Meta symbols as same as DNA or RNA representations. System architecture of key components and core mechanism on the VMS are described. Key modules, equations and their I/O parameters are discussed. Applying the VM System, two sets of real DNA sequences from both sample human (noncoding DNA) and corn (coding DNA) genomes are collected in comparison with pseudo DNA sequences generated by HC-256 to show their intrinsic properties in higher levels of similar relationships among relevant DNA sequences on 2D maps. Sample 2D maps are listed and their characteristics are illustrated under controllable environment. Visual results are briefly analyzed to explore their intrinsic properties on selected genome sequences. 展开更多
关键词 PSEUDO-RANDOM Number Generator STREAM CIPHER HC-256 Binary to dna Pseudo dna sequence Large Noncoding dna analysis 2D MAP Visual Distribution VARIANT MAP System
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A TENSOR ANALYSIS METHOD FOR ESTIMATING 3-D MOTION PARAMETERS
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作者 李象霖 陈小平 《Journal of Electronics(China)》 1992年第3期209-217,共9页
By using the center projection image sequence to estimate 3-D motion parameters,one needs to know the corresponding relationship between the feature of motion object in spaceand the projection coordinate on image plan... By using the center projection image sequence to estimate 3-D motion parameters,one needs to know the corresponding relationship between the feature of motion object in spaceand the projection coordinate on image plane.In order to avoid using the relationship of featurecorrespondence,the tensor analysis method in the affine transformation system is presented,andthe simulation data of experimental results are given. 展开更多
关键词 SIGNAL processing Image sequence analysis 3-D motion analysis TENSOR analysis method
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DNA sequence representation by trianders and determinative degree of nucleotides
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作者 DUPLIJ Diana DUPLIJ Steven 《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 SCIE CAS CSCD 2005年第8期743-755,共13页
A new version of DNA walks, where nucleotides are regarded unequal in their contribution to a walk is introduced, which allows us to study thoroughly the “fine structure” of nucleotide sequences. The approach is bas... A new version of DNA walks, where nucleotides are regarded unequal in their contribution to a walk is introduced, which allows us to study thoroughly the “fine structure” of nucleotide sequences. The approach is based on the assumption that nucleotides have an inner abstract characteristic, the determinative degree, which reflects genetic code phenomenological prop-erties and is adjusted to nucleotides physical properties. We consider each codon position independently, which gives three separate walks characterized by different angles and lengths, and that such an object is called triander which reflects the “strength” of branch. A general method for identifying DNA sequence “by triander” which can be treated as a unique “genogram” (or “gene passport”) is proposed. The two- and three-dimensional trianders are considered. The difference of sequences fine structure in genes and the intergenic space is shown. A clear triplet signal in coding sequences was found which is absent in the intergenic space and is independent from the sequence length. This paper presents the topological classification of trianders which can allow us to provide a detailed working out signatures of functionally different genomic regions. 展开更多
关键词 dna walk Triander Determinative degree analysis dna sequences DYSTROPHIN NUCLEOTIDE
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Pseudo DNA Sequence Generation of Non-Coding Distributions Using Variant Maps on Cellular Automata
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作者 Jeffrey Zheng Jin Luo Wei Zhou 《Applied Mathematics》 2014年第1期153-174,共22页
In a recent decade, many DNA sequencing projects are developed on cells, plants and animals over the world into huge DNA databases. Researchers notice that mammalian genomes encoding thousands of large noncoding RNAs ... In a recent decade, many DNA sequencing projects are developed on cells, plants and animals over the world into huge DNA databases. Researchers notice that mammalian genomes encoding thousands of large noncoding RNAs (lncRNAs), interact with chromatin regulatory complexes, and are thought to play a role in localizing these complexes to target loci across the genome. It is a challenge target using higher dimensional tools to organize various complex interactive properties as visual maps. In this paper, a Pseudo DNA Variant MapPDVM is proposed following Cellular Automata to represent multiple maps that use four Meta symbols as well as DNA or RNA representations. The system architecture of key components and the core mechanism on the PDVM are described. Key modules, equations and their I/O parameters are discussed. Applying the PDVM, two sets of real DNA sequences from both the sample human (noncoding DNA) and corn (coding DNA) genomes are collected in comparison with two sets of pseudo DNA sequences generated by a stream cipher HC-256 under different modes to show their intrinsic properties in higher levels of similar relationships among relevant DNA sequences on 2D maps. Sample 2D maps are listed and their characteristics are illustrated under a controllable environment. Various distributions can be observed on both noncoding and coding conditions from their symmetric properties on 2D maps. 展开更多
关键词 Large Noncoding dna analysis Stream CIPHER HC-256 Binary to dna PSEUDO dna sequence Visual Distribution VARIANT Map
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STUDY ON THE SEQUENCE STRUCTURE OF SBR BY ^(13)C-NMR METHOD Ⅱ. PEAK ASSIGNMENT FOR ALIPHATIC CARBONS SPECTRA
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作者 焦书科 陈晓农 +1 位作者 胡力平 严宝珍 《Chinese Journal of Polymer Science》 SCIE CAS CSCD 1990年第1期25-35,共11页
The study on ^(13)C-NMR spectra of aliphatic carbon region of emuision-processed and solution-processed (by lithium catalyst) SBR was carried out. The assignments for more than thirty odd peaks observed experimentally... The study on ^(13)C-NMR spectra of aliphatic carbon region of emuision-processed and solution-processed (by lithium catalyst) SBR was carried out. The assignments for more than thirty odd peaks observed experimentally were made by using 'corresponding analysis' method, combined with the empirical parameters reported in literature. The peak intensifies were calculated based on BemouUian statistic assumption. 展开更多
关键词 Corresponding analysis method peak assignment for aliphatic carbon region sequence distribution of SBR empirical parameter method.
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Sequencing of hepatitis C virus cDNA with polymerase chain reaction directed sequencing *
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作者 魏来 王宇 +1 位作者 陈红松 陶其敏 《World Journal of Gastroenterology》 SCIE CAS CSCD 1997年第1期18+15-17,15-17,共4页
AIM To explore a rapid and easy sequencing method for hepatitis C virus (HCV) genome, and establish a new sequencing method in China. METHODS Polymerase Chain Reaction (PCR) was combined with DNA sequencing techn... AIM To explore a rapid and easy sequencing method for hepatitis C virus (HCV) genome, and establish a new sequencing method in China. METHODS Polymerase Chain Reaction (PCR) was combined with DNA sequencing technique. PCR products were purified by agarose gel electrophoresis, polyacrylamide gel electrophoresis (PAGE), Polyethylene glycol (PEG) respectively. Then in the presence of a 5′ labeling PCR primer, purified PCR products were directly sequenced. By this method, HCV NS5b cDNA from two HCV infected individuals (HC 42 and HC 49) were sequenced. 展开更多
关键词 Hepatitis C virus dna viral dna complementary Polymerase chain reaction sequence analysis dna Mutation
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