Barley(Hordeum vulgare L.)is one of the most Aluminum(Al)sensitive cereal species.In this study,the physiological,biochemical,and molecular response of barley seedlings to Al treatment was examined to gain insight int...Barley(Hordeum vulgare L.)is one of the most Aluminum(Al)sensitive cereal species.In this study,the physiological,biochemical,and molecular response of barley seedlings to Al treatment was examined to gain insight into Al response and tolerance mechanisms.The results showed that superoxide dismutase(SOD),peroxidase(POD)and catalase(CAT)activity were inhibited to different degrees following Al exposure.The MDA content also significantly increased with increasing Al concentrations.SRAP results indicated significant differences between Al treatments and controls in terms of SRAP profile,and the genomic template stability(GTS)decreased with increasing Al concentration and duration.These integrative results help to elucidate the underlying mechanisms that the barley response to Al toxicity.展开更多
In order to evaluation the efficiency of SRAP markers on genetic diversity of Aspergillus flavus,we screened out 17sets of primer pairs which could produce clear and reproducible SRAP bands from 150 SRAP primer pairs....In order to evaluation the efficiency of SRAP markers on genetic diversity of Aspergillus flavus,we screened out 17sets of primer pairs which could produce clear and reproducible SRAP bands from 150 SRAP primer pairs.The size of SRAP fragments ranged from 120 to 2100 bp.Primer pair Me10/Em9 produced the maximum number of polymorphic bands(12 bands),while Me8/Em13 produced the fewest number of polymorphic bands(only 1).Through analysis genetic diversity ability of different sets of primer pairs,the set of 12 primer pairs was selected for SRAP genetic marker of A.flavus.Cluster analysis was performed based on the genetic similarity coefficients,which ranged from 0.53 to 0.89.A dendrogram assembled using the unweighted pair-group method with arithmetic averages grouped A.flavus samples into 5 main clusters.The results suggested that SRAP marker is a useful molecular technology for the diversity of A.flavus from peanut soils in China.展开更多
Using SRAP (sequence-related amplified polymorphism) markers a genetic linkage map of cucumber was constructed with a population consisting of 138 F2 individuals derived from a cross of the two cucumber lines, S06 and...Using SRAP (sequence-related amplified polymorphism) markers a genetic linkage map of cucumber was constructed with a population consisting of 138 F2 individuals derived from a cross of the two cucumber lines, S06 and S52. In the survey of parental polymorphisms with 182 primer combinations, 64 polymorphism-revealing primer pairs were screened out, which generated totally 108 polymorphic bands with an average of 1.7 bands per primer pair and at most 6 bands from one primer pair. The constructed molecular linkage map included 92 loci, distributed in seven linkage groups and spanning 1164.2 cM in length with an average genetic distance of 12.6 cM between two neighboring loci. Based on this linkage map, the quantitative trait loci (QTL) for the lateral branch number (lbn) and the lateral branch average length (lbl) in cucumber were identified by QTLMapper1.6. A major QTL lbn1 located between ME11SA4B and ME5EM5 in LG2 could explain 10.63% of the total variation with its positively effecting allele from S06. A major QTL lbl1 located between DC1OD3 and DC1EM14 in LG2 could account for 10.38% of the total variation with its positively effecting allele from S06.展开更多
基金This research was funded by National Key Technology Research and Development Program(2015BAD01B02)the National Natural Science Foundation of China(31401316).
文摘Barley(Hordeum vulgare L.)is one of the most Aluminum(Al)sensitive cereal species.In this study,the physiological,biochemical,and molecular response of barley seedlings to Al treatment was examined to gain insight into Al response and tolerance mechanisms.The results showed that superoxide dismutase(SOD),peroxidase(POD)and catalase(CAT)activity were inhibited to different degrees following Al exposure.The MDA content also significantly increased with increasing Al concentrations.SRAP results indicated significant differences between Al treatments and controls in terms of SRAP profile,and the genomic template stability(GTS)decreased with increasing Al concentration and duration.These integrative results help to elucidate the underlying mechanisms that the barley response to Al toxicity.
基金the grants from the Natural Science Foundation of Shandong Province(ZR2020MC103,ZR2021MC040)Innovation Project of Agricultural Science and Technology of Shandong Academy of Agricultural Sciences(CXGC2022B06,CXGC2022F33)。
文摘In order to evaluation the efficiency of SRAP markers on genetic diversity of Aspergillus flavus,we screened out 17sets of primer pairs which could produce clear and reproducible SRAP bands from 150 SRAP primer pairs.The size of SRAP fragments ranged from 120 to 2100 bp.Primer pair Me10/Em9 produced the maximum number of polymorphic bands(12 bands),while Me8/Em13 produced the fewest number of polymorphic bands(only 1).Through analysis genetic diversity ability of different sets of primer pairs,the set of 12 primer pairs was selected for SRAP genetic marker of A.flavus.Cluster analysis was performed based on the genetic similarity coefficients,which ranged from 0.53 to 0.89.A dendrogram assembled using the unweighted pair-group method with arithmetic averages grouped A.flavus samples into 5 main clusters.The results suggested that SRAP marker is a useful molecular technology for the diversity of A.flavus from peanut soils in China.
基金This work was support ed by the Shanghai Municipal Scientifi c&Technological Commission(Grant Nos.033107019)Shanghai Municipal Agricultural Commission(Grant Nos 2003-2-1-2).
文摘Using SRAP (sequence-related amplified polymorphism) markers a genetic linkage map of cucumber was constructed with a population consisting of 138 F2 individuals derived from a cross of the two cucumber lines, S06 and S52. In the survey of parental polymorphisms with 182 primer combinations, 64 polymorphism-revealing primer pairs were screened out, which generated totally 108 polymorphic bands with an average of 1.7 bands per primer pair and at most 6 bands from one primer pair. The constructed molecular linkage map included 92 loci, distributed in seven linkage groups and spanning 1164.2 cM in length with an average genetic distance of 12.6 cM between two neighboring loci. Based on this linkage map, the quantitative trait loci (QTL) for the lateral branch number (lbn) and the lateral branch average length (lbl) in cucumber were identified by QTLMapper1.6. A major QTL lbn1 located between ME11SA4B and ME5EM5 in LG2 could explain 10.63% of the total variation with its positively effecting allele from S06. A major QTL lbl1 located between DC1OD3 and DC1EM14 in LG2 could account for 10.38% of the total variation with its positively effecting allele from S06.
