期刊文献+
共找到1篇文章
< 1 >
每页显示 20 50 100
The expressions of brain-derived neurotrophic factor and nerve growth factor in purified rat choroid plexus epithelial cells in vitro 被引量:1
1
作者 CHEN Bo MIAO Xingyu +4 位作者 SHI Wei PU Jingnan LIU Chongxiao GUO Zhenyu WANG Fangru 《Journal of Medical Colleges of PLA(China)》 CAS 2013年第5期257-267,共11页
Objective: To detect the expressions of brain-derived neurotrophic factor (BDNF) and nerve growth factor (NGF) in purified rat choroid plexus epithelial cells in vitro. Methods: Primary and passage choroid plexu... Objective: To detect the expressions of brain-derived neurotrophic factor (BDNF) and nerve growth factor (NGF) in purified rat choroid plexus epithelial cells in vitro. Methods: Primary and passage choroid plexus epithelial cells were obtained from newborn, one-day Spragne-Dawley rats. The expressions of BDNF and NGF were measured by qRT-PCR and Western blottingting. The secretions of BDNF and NGF were detected by ELISA. Cell supematants of primary cells, purified cells and passage 1 cells were harvested. Results: The expression of BDNF in the purified cells was significantly lower than that in the primary cells (P〈0.05), and it in the primary cells and the purified cells was significantly higher than that in the passage 1 cells (P〈0.05). The expression of NGF was significantly higher in the purified cells than in the primary cells and the passage 1 cells (P〈0.05). It in the passage 1 cells was significantly higher than that in the primary cells (P〈0.05). Conclusion: The time of CPECs transplantation for central nervous system diseases should be selected based on their secretory function and features,which could lead to better and more effective treatment. 展开更多
关键词 Brain derived-neurotrophic factor Nerve growth factor Choroid plexus epithefial cells Primary culture Passage culture serial subcultivation
下载PDF
上一页 1 下一页 到第
使用帮助 返回顶部