Myogenic Regulatory Factors(MRFs)is involved in the muscle growth and differentiation.In this study,the cDNA sequence of yellowtail kingfish MRFs genes were cloned by rapid amplification of cDNA ends(RACE)method;then,...Myogenic Regulatory Factors(MRFs)is involved in the muscle growth and differentiation.In this study,the cDNA sequence of yellowtail kingfish MRFs genes were cloned by rapid amplification of cDNA ends(RACE)method;then,the character-istics of these genes and the predicted protein sequences were analyzed by bioinformatics methods,the tissue and embryonic stages differential expression pattern were detected by the quantitative real-time PCR.Our results showed that the yellowtail kingfish(YTK)Myf5 cDNA has a full length of 951 bp,encoding 266 amino acids.The yellowtail kingfish Myf6 cDNA has a full length of 1105bp,encoding 250 amino acids.The proteins containα-helix,β-strand,and loops.The Neighbour-joining tree revealed that YTK Myf5 and Myf6 are closely related to Seriola dumerili.The yellowtail kingfish Myf5 and Myf6 gene expressed significantly higher in mus-cle than in other tissues(P<0.05).In addition,Myf5 and Myf6 in muscle was significantly expressed in 400g and 500 g fish but not in 50 g,suggesting that myogenic regulatory factors expression had a great relationship with the fish size.Our results also indicated that Myf5 and Myf6 have different functions during embryonic development,because Myf5 showed highest expression level at the neuroembryo period,but Myf6 had the highest expression level at embryo coverage yolk 70%stage.Myf5 gene showed highest ex-pression at 30 d of age,suggesting it played key roles in myogenic period.However,the Myf6 gene was significantly highly ex-pressed at 60 d,revealing this gene functioned in the later muscle formation period.展开更多
The aquaculture of yellowtail kingfish(Seriola lalandi)has expanded around the globe,including China.Genetic resource of Chinese S.lalandi urgently needs to be assessed for improving production.Here,we collected wild ...The aquaculture of yellowtail kingfish(Seriola lalandi)has expanded around the globe,including China.Genetic resource of Chinese S.lalandi urgently needs to be assessed for improving production.Here,we collected wild S.lalandi samples from the Bohai Sea,China and evaluated its genetic diversity based on 17,690 nuclear loci.A population from the Southern Ocean,Australia was used for comparison.The analyses showed that the Chinese and Australian S.lalandi formed two completely distinct clusters and there was no genetic introgression from the Australian S.lalandi into the Chinese S.lalandi population.The genetic diversity is slightly lower,but comparable in Chinese versus Australian yellowtail kingfish(Chinese population,expected heterozygosity:0.19,observed heterozygosity:0.19,nucleotide diversity:0.19±0.09;Australian population,expected heterozygosity:0.23,observed heterozygosity:0.22,nucleotide diversity:0.22±0.11).Overall,our results indicated that Chinese S.lalandi could be a potential subject for genetic breeding programs.We also investigated morphological characters and developed molecular markers for population identification.Comparison of meristic characters between the Chinese population and the Australian population revealed that main shape difference were in the number of dorsal spines,dorsal fin rays and the number of upper gill rakers.Geometric morphology based on eight landmarks also revealed significant difference between the two populations including the distance between the tip of snout to origin of pelvic fin and the distance between the tip of snout to origin of pectoral fin.These morphological characters can be used for easy identification of the Chinese S.lalandi.展开更多
基金the National Key Research and Development Program(Nos.2018YFD0901204 and 2019YFD0900503)the National Natural Science Foun-dation of China(No.31772829)+4 种基金the Laboratory for Ma-rine Fisheries Science and Food Production Processes,Qingdao National Laboratory for Marine Science and Technology,P.R.China(No.2017-3A01)Central Pub-lic-interest Scientific Institution Basal Research,CAFS&Key Laboratory of Sustainable Development of Marine Fisheries,Ministry of Agriculture and Rural Affairs,P.R.China(No.2019HY-XKQ01)the Central Public-interest Scientific Institution Basal Research Fund,YSFRI,CAFS(No.20603022021004)the Central Public-interest Scien-tific Institution Basal Research fund,CAFS(No.47)the China Agriculture Research System(No.CARS-47).
文摘Myogenic Regulatory Factors(MRFs)is involved in the muscle growth and differentiation.In this study,the cDNA sequence of yellowtail kingfish MRFs genes were cloned by rapid amplification of cDNA ends(RACE)method;then,the character-istics of these genes and the predicted protein sequences were analyzed by bioinformatics methods,the tissue and embryonic stages differential expression pattern were detected by the quantitative real-time PCR.Our results showed that the yellowtail kingfish(YTK)Myf5 cDNA has a full length of 951 bp,encoding 266 amino acids.The yellowtail kingfish Myf6 cDNA has a full length of 1105bp,encoding 250 amino acids.The proteins containα-helix,β-strand,and loops.The Neighbour-joining tree revealed that YTK Myf5 and Myf6 are closely related to Seriola dumerili.The yellowtail kingfish Myf5 and Myf6 gene expressed significantly higher in mus-cle than in other tissues(P<0.05).In addition,Myf5 and Myf6 in muscle was significantly expressed in 400g and 500 g fish but not in 50 g,suggesting that myogenic regulatory factors expression had a great relationship with the fish size.Our results also indicated that Myf5 and Myf6 have different functions during embryonic development,because Myf5 showed highest expression level at the neuroembryo period,but Myf6 had the highest expression level at embryo coverage yolk 70%stage.Myf5 gene showed highest ex-pression at 30 d of age,suggesting it played key roles in myogenic period.However,the Myf6 gene was significantly highly ex-pressed at 60 d,revealing this gene functioned in the later muscle formation period.
基金This work was supported by Shanghai Collaborative Innovation for Aquatic Animal Genetics and Breeding.
文摘The aquaculture of yellowtail kingfish(Seriola lalandi)has expanded around the globe,including China.Genetic resource of Chinese S.lalandi urgently needs to be assessed for improving production.Here,we collected wild S.lalandi samples from the Bohai Sea,China and evaluated its genetic diversity based on 17,690 nuclear loci.A population from the Southern Ocean,Australia was used for comparison.The analyses showed that the Chinese and Australian S.lalandi formed two completely distinct clusters and there was no genetic introgression from the Australian S.lalandi into the Chinese S.lalandi population.The genetic diversity is slightly lower,but comparable in Chinese versus Australian yellowtail kingfish(Chinese population,expected heterozygosity:0.19,observed heterozygosity:0.19,nucleotide diversity:0.19±0.09;Australian population,expected heterozygosity:0.23,observed heterozygosity:0.22,nucleotide diversity:0.22±0.11).Overall,our results indicated that Chinese S.lalandi could be a potential subject for genetic breeding programs.We also investigated morphological characters and developed molecular markers for population identification.Comparison of meristic characters between the Chinese population and the Australian population revealed that main shape difference were in the number of dorsal spines,dorsal fin rays and the number of upper gill rakers.Geometric morphology based on eight landmarks also revealed significant difference between the two populations including the distance between the tip of snout to origin of pelvic fin and the distance between the tip of snout to origin of pectoral fin.These morphological characters can be used for easy identification of the Chinese S.lalandi.
