Serological survey on canine coronavirus antibodies in giant pandas by virus neutralization test

In order to survey the infectious situation of canine coronavirus (CCV) in giant panda population, a virus neutralization test detecting specific antibodies against CCV in giant panda抯 sera was established by using two-fold dilutions of serum and 100 TCID50 of the virus. The 62 sera samples of giant pandas, which were gathered from zoos and reserve region of Sichuan Province, China were detected. The neutralization antibody titer of 1:4 was recognized as the positive criterion, 8 sera samples were detected to be positive, and the positive rate was 12.9%. The titers of neutralizing antibody ranged from 1:8 to 1:32. It was the first comprehensive investigation on neutralization antibodies against CCV in giant panda population in China. The results of study showed that the infection of CCV in giant panda population was universal, which has posed a threat to the health of giant panda. Therefore, it is incumbent on us to study safe and effective vaccines to protect giant panda against CCV infection.

Serological Survey of Zika Virus in Humans and Animals in Dejiang Prefecture, Guizhou Province,China

Objective The current outbreak of Zika virus(ZIKV)poses a severe threat to human health.Two ZIKV strains were isolated from mosquitoes collected from the Dejiang prefecture in China in 2016,which was the first isolation of ZIKV in nature in China.Methods In this study,serum samples were collected from 366 healthy individuals and 104 animals from Dejiang prefecture in 2017,and the plaque reduction neutralization test(PRNT)was used to evaluate the seroprevalence of ZIKV.Results None of the 366 residents from whom the samples were collected were seropositive for ZIKV.None of the 11 pigs from whom the samples were collected were seropositive for ZIKV,while 1 of 63(1.59%)chickens and 2 of 30(6.67%)sheep were seropositive for ZIKV.Conclusions The extremely low seropositivity rate of ZIKV antibodies in animals in the Dejiang prefecture,Guizhou province in this study indicates that ZIKV can infect animals;however,there is a low risk of ZIKV circulating in the local population.

A Serological Survey of Contagious Caprine Pleuropneumonia in Taizhou City,Jiangsu Province

[ Objective] This study aimed to investigate the prevalence of contagious caprine pleuropneumonia in Taizhou City, Jiangsu Province. [ Method] By using indirect hemagglutination assay kits for detection of antibodies against Mycoplasma capricolum subsp, capripneumoniae （ Mecp）, Mycoplasma mycoides subsp. capriclonum （Mmc） and Mycopalsam ovipneumonia （ Movi）, 1 157 goat sera from Taizhou City of Jiangsu Province were detected. [ Result ] The average positive detection rate of Mccp and Mmc in infected goats was 51.85% and 39.81%, respectively; the average positive detection rate of Mccp and Mmc in non-infected goats was 10.52% and 5.84%, respectively, and the total positive detection rate of Mycoplasma infections was 16.37%. [ Conclusion ] Mycoplasma infections oc- cur commonly in Taizhou City. The prevention and control of Mycoplasma infections should be strengthened.

Bluetongue Virus （BTV） Serological Survey and Evidence of Emergent BTV-8 Serotype in Morocco

Bluetongue （BT） is an infectious, non-contagious arthropod-borne disease that infects all ruminants, including sheep, cattle, deer, goats and camelids. Bluetongue virus （BTV） belonged to Reoviridae is ARN genome of 19 Kb. Twenty-six BTV serotypes have long been recognized, to be associated with severe disease in certain breeds of sheep, whereas cattle and goats are usually sub-clinically affected. Before 2004, BT was considered an exotic disease in Morocco, however, the first outbreak was observed in 2004 in sheep. This outbreak was caused by the isolated BTV-4. Two years later a BTV-1 emerged in Morocco. Both serotype 1 and serotype 4 circulated after 2007 across the country. The aims of the present work was to perform a serological study on sheep from different regions in Morocco in order to clarify the current BTV epidemiological situation and its evolution from 2009 to 2012, to determine the co-infection rate, and to confirm the possible circulation of other BTV Serotype mainly the BTV-8. All of 436 sera were tested by serum neutralization using reference strains. Results confirm the presence of BTV-4, BTV-1 and BTV-8. However, the present study report for the first time the emerging BTV-8 circulation in Morocco. Moreover, the founding reveal as well a higher co-infection rate in cattle compared to sheep.