以耐铬(VI)菌株Serratia sp. CM01为研究对象,探究其全基因组信息,挖掘其潜在的铬代谢相关基因。本研究采用基因组测序技术对CM01进行全基因组测序并分析其基因序列特征;同时,结合前期差异蛋白研究结果,进行铬代谢相关基因分析。测序结...以耐铬(VI)菌株Serratia sp. CM01为研究对象,探究其全基因组信息,挖掘其潜在的铬代谢相关基因。本研究采用基因组测序技术对CM01进行全基因组测序并分析其基因序列特征;同时,结合前期差异蛋白研究结果,进行铬代谢相关基因分析。测序结果表明,CM01基因组大小为4,902,254 bp,预测编码蛋白序列的基因有4547个;蛋白功能注释结果显示其涉及氧化还原、氨基酸代谢、碳水化合物和能量代谢编码的基因有较高的占比。结合前期蛋白组学的结果,筛选出了12个与铬代谢相关的基因。qRT-PCR分析结果显示,在Cr(VI)胁迫下,ChrA1、Srpc、GrxA和NemA基因的表达上调。CM01基因组全序列已上传至NCBI,序列号:PRJNA675313。本研究通过对CM01的基因组序列分析,为全面了解细菌的铬代谢机制提供基础,为修复环境铬污染的新生物技术提供理论依据。The study focused on the chromium (VI)-resistant bacterial strain Serratia sp. CM01 to explore its whole-genome information and to mine its potential chromium metabolism-related genes. In this research, genomic sequencing technology was employed to perform whole-genome sequencing on CM01 and to analyze its gene sequence characteristics. Additionally, the results from previous differential protein studies were integrated to analyze genes related to chromium metabolism. The sequencing results indicated that the CM01 genome is 4,902,254 base pairs in size, with 4547 genes predicted to encode protein sequences. Protein function annotation revealed a high proportion of genes involved in oxidation-reduction, amino acid metabolism, carbohydrate metabolism, and energy metabolism. Combining the outcomes from previous proteomics studies, 12 genes related to chromium metabolism were identified. Quantitative real-time PCR analysis showed that the expression of ChrA1, Srpc, GrxA, and NemA genes was upregulated under Cr(VI) stress. The complete genome sequence of CM01 has been uploaded to NCBI with the accession number PRJNA675313. Through the genomic sequence analysis of CM01, this study provides a foundation for a comprehensive understanding of bacterial chromium metabolism mechanisms and offers a theoretical basis for the development of new biotechnologies for the remediation of environmental chromium pollution.展开更多
Quorum sensing(Qs)inhibition has emerged as a promising target for directed drug design,providing an appealing strategy for developing antimicrobials,particularly against infections caused by drug-resistant pathogens....Quorum sensing(Qs)inhibition has emerged as a promising target for directed drug design,providing an appealing strategy for developing antimicrobials,particularly against infections caused by drug-resistant pathogens.In this study,we designed and synthesized a total of 33β-nitrostyrene derivatives using 1-nitro-2-phenylethane(NPe)as the lead compound,to target the facultative anaerobic bacterial pathogen Serratia marcescens.The QS-inhibitory effects of these compounds were evaluated using S.marcescens NJ01 and the reporter strain Chromobacterium violaceum CV026.Among the 33 newβ-nitrostyrene derivatives,(E)-1-methyl-4-(2-nitrovinyl)benzene(m-NPe,compound 28)was proven to be a potent inhibitor that reduced biofilm formation of S.marcescens NJ01 by 79%.Scanning electron microscopy(SEM)and confocal laser scanning microscopy(CLSM)results revealed that treatment with m-NPe(50μg/ml)not only enhanced the susceptibility of the formed biofilms but also disrupted the architecture of biofilms by 84%.m-NPe(50μg/ml)decreased virulence factors in S.marcescens NJ01,reducing the activity of protease,prodigiosin,and extracellular polysaccharide(EPs)by 36%,72%,and 52%,respectively.In S.marcescens 4547,the activities of hemolysin and EPs were reduced by 28%and 40%,respectively,outperforming the positive control,vanillic acid(VAN).The study also found that the expression levels of QS-and biofilm-related genes(flhD,fimA,fimC,sodB,bsmB,pigA,pigC,and shlA)were downregulated by 1.21-to 2.32-fold.Molecular dynamics analysis showed that m-NPe could bind stably to SmaR,Rhll,RhiR,LasR,and CviR proteins in a 0.1 M sodium chloride solution.Importantly,a microscale thermophoresis(MST)test revealed that SmaR could be a target protein for the screening of a quorum sensing inhibitor(QSl)against S.marcescens.Overall,this study highlights the efficacy of m-NPe in suppressing the virulence factors of S.marcescens,identifying it as a new potential Qsl and antibiofilm agent capable of restoring or improving antimicrobial drug sensitivity.展开更多
Serratia is an opportunistic pathogenic bacterium of the Enterobacteriaceae family, occasionally responsible for epidemics of nosocomial infections in critical departments;in particular in neonatal intensive care unit...Serratia is an opportunistic pathogenic bacterium of the Enterobacteriaceae family, occasionally responsible for epidemics of nosocomial infections in critical departments;in particular in neonatal intensive care units Enterobacteriaceae form a large family of Gram-negative bacteria, which cause diseases of highly variable severity, due to distinct pathogenic mechanisms. This family is heterogeneous as it consists of about 30 genera of bacteria and more than 100 species. However, all these germs have in common their preferential location in the digestive system, some being part of the normal flora although they are also present in the environment. Several metabolic processes characterize this bacterial family. These include the ability to reduce nitrate to nitrite (for energy generation), ferment glucose, lack cytochrome oxidase, be aerobic or anaerobic, motile or immobile, … Enterobacteriaceae constitute more than 80% of the germs isolated in the laboratory: Escherichia, Salmonella, Shigella, Klebsiella, Enterobacter, Serratia, Proteus, Morganella and Yersinia are the rods most often found. Regarding nosocomial infection to Serratia, this bacterium colonizes the respiratory, digestive and urinary systems of patients, mainly responsible for bacteremia, infections of the lower respiratory tract, urinary and skin infections. Through our work, we report the epidemic experienced in the neonatal intensive care unit MOHAMMED VI university hospital, mother-child hospital MARRAKECH MOROCCO for three months from December at February 2023 interesting 30 newborns whose clinical presentation was different, the positive diagnosis was based on blood and geographical samples taken by the bacteriology department to isolate the offending germs, the therapeutic management of our patients consisted essentially of various hygiene measures in association with dual antibiotic therapy based on meropenem and amikacin. The evolution was marked by the death of 21 patients (70%) and the recovery of 9 patients (30%). The prognosis of infection by serratia remains bleak with high morbidity interest in prevention by respecting the rules of hygiene, which begins with hand disinfection.展开更多
We present the 3<sup>rd</sup> known case of a Serratia rubidaea urinary tract infection in a 49-year-old male with past medical history of bilateral lower extremity paraplegia who presented to the emergenc...We present the 3<sup>rd</sup> known case of a Serratia rubidaea urinary tract infection in a 49-year-old male with past medical history of bilateral lower extremity paraplegia who presented to the emergency room with fever, lethargy, and red tinted urine. The Serratia genus, of the family Enterobacteracia, in particular the species marcescens, are important causes of infection in humans, animals, and insects, however, until the mid-1950s, this was not the case, and the organism was considered non-pathogenic and frequently used in medical experiments. Serratia are facultatively anaerobic, gram-negative rods, the majority with peritrichous flagella primarily inhabiting soil, water, and plant surfaces. Serratia marcescens is now a known pathogen, however, less frequently isolated species, including Serratia rubidaea, are worthy of discussion, especially due to its characteristic red color and rarity. We aim to increase the awareness Serratia rubidaea including its presentation, inherent antimicrobial resistance, and treatment options.展开更多
从大豆土壤中分离纯化得到一株具有卵磷脂和乐果降解能力的菌株Yj1,对该菌株进行鉴定、生长条件优化、酶活性鉴定以及有机磷降解酶的分离纯化。结果表明,Yj1与Serratia marcescens WW4(CP003959.1)的16S r DNA相似度为99%。正交试验对...从大豆土壤中分离纯化得到一株具有卵磷脂和乐果降解能力的菌株Yj1,对该菌株进行鉴定、生长条件优化、酶活性鉴定以及有机磷降解酶的分离纯化。结果表明,Yj1与Serratia marcescens WW4(CP003959.1)的16S r DNA相似度为99%。正交试验对所需培养基进行优化,得到该菌株的最佳生长条件为甘露糖、蛋白胨和p H 8的组合。Yj1菌株在两种磷源条件下,菌株生长量均很低,但72 h内以大豆卵磷脂为磷源时的菌体生长情况优于乐果。以大豆卵磷脂为磷源时酸性磷酸酶、碱性磷酸酶与有机磷降解酶活性明显高于以乐果为磷源时的酶活,且72 h内碱性磷酸酶活性一直都高于酸性磷酸酶和有机磷降解酶。硫酸铵沉淀法结合阳离子交换层析成功从Yj1菌体中分离纯化了有机磷降解酶,SDS-PAGE结果显示纯化的蛋白为单一条带。且阳离子交换层析的提纯倍数是硫酸氨沉淀的5.303倍,硫酸氨沉淀为粗酶的1.416倍。展开更多
A total of 479 bacterial strains were isolated from brine （Bohai, Qinhuangdao City, Hebei Province, China）. Bioassay results indicated that 4 strains named Hal, Hal7, Ha38, and Ha384 had herbicidal activity. And str...A total of 479 bacterial strains were isolated from brine （Bohai, Qinhuangdao City, Hebei Province, China）. Bioassay results indicated that 4 strains named Hal, Hal7, Ha38, and Ha384 had herbicidal activity. And strain Hal had the highest effective herbicidal activity. As a result, this study aims to JdentJfy strain Hal, characterize its physiological and biological activities, evaluate the herbicidal activity of its metabolites, and develop a ＇pesta＇ formulation and assess its effectiveness on Digitaria sanguinalis. Hal was identified as Serratia marcescens based on 16S rDNA sequencing. This strain has a flagellum, a diameter of 0.5 to 0.8 IJm, and a length of 0.9 to 2.0 IJm. The indole test shows positive results, and the catalase enzyme exhibits strong positive reactions. Results further showed that the inhibitory concentration （IC50） of the crude extracts to D. sanguinalis radicula and coleoptile were 3.332 and 2.828 mg mL-1, respectively. Both the suppression of D. sanguinalis and the cell viability of the Hal formulation in ＇pesta＇ were higher when stored at 4℃ than at （25＋2）℃. These results indi- cated that S. marcescens Hal can potentially be used as a biocontrol agent against D. sanguinalis.展开更多
文摘以耐铬(VI)菌株Serratia sp. CM01为研究对象,探究其全基因组信息,挖掘其潜在的铬代谢相关基因。本研究采用基因组测序技术对CM01进行全基因组测序并分析其基因序列特征;同时,结合前期差异蛋白研究结果,进行铬代谢相关基因分析。测序结果表明,CM01基因组大小为4,902,254 bp,预测编码蛋白序列的基因有4547个;蛋白功能注释结果显示其涉及氧化还原、氨基酸代谢、碳水化合物和能量代谢编码的基因有较高的占比。结合前期蛋白组学的结果,筛选出了12个与铬代谢相关的基因。qRT-PCR分析结果显示,在Cr(VI)胁迫下,ChrA1、Srpc、GrxA和NemA基因的表达上调。CM01基因组全序列已上传至NCBI,序列号:PRJNA675313。本研究通过对CM01的基因组序列分析,为全面了解细菌的铬代谢机制提供基础,为修复环境铬污染的新生物技术提供理论依据。The study focused on the chromium (VI)-resistant bacterial strain Serratia sp. CM01 to explore its whole-genome information and to mine its potential chromium metabolism-related genes. In this research, genomic sequencing technology was employed to perform whole-genome sequencing on CM01 and to analyze its gene sequence characteristics. Additionally, the results from previous differential protein studies were integrated to analyze genes related to chromium metabolism. The sequencing results indicated that the CM01 genome is 4,902,254 base pairs in size, with 4547 genes predicted to encode protein sequences. Protein function annotation revealed a high proportion of genes involved in oxidation-reduction, amino acid metabolism, carbohydrate metabolism, and energy metabolism. Combining the outcomes from previous proteomics studies, 12 genes related to chromium metabolism were identified. Quantitative real-time PCR analysis showed that the expression of ChrA1, Srpc, GrxA, and NemA genes was upregulated under Cr(VI) stress. The complete genome sequence of CM01 has been uploaded to NCBI with the accession number PRJNA675313. Through the genomic sequence analysis of CM01, this study provides a foundation for a comprehensive understanding of bacterial chromium metabolism mechanisms and offers a theoretical basis for the development of new biotechnologies for the remediation of environmental chromium pollution.
基金This work was supported by the National Natural Science Foundation of China(Nos.82160664 and 31760246)the Hainan Province Science and Technology Special Fund(ZDYF2024SHFZ103)+1 种基金the Natural Science Foundation of Hainan Province(222RC557 and 221QN170)Anhui Laboratory of Molecule-Based Materials(fzj21006).
文摘Quorum sensing(Qs)inhibition has emerged as a promising target for directed drug design,providing an appealing strategy for developing antimicrobials,particularly against infections caused by drug-resistant pathogens.In this study,we designed and synthesized a total of 33β-nitrostyrene derivatives using 1-nitro-2-phenylethane(NPe)as the lead compound,to target the facultative anaerobic bacterial pathogen Serratia marcescens.The QS-inhibitory effects of these compounds were evaluated using S.marcescens NJ01 and the reporter strain Chromobacterium violaceum CV026.Among the 33 newβ-nitrostyrene derivatives,(E)-1-methyl-4-(2-nitrovinyl)benzene(m-NPe,compound 28)was proven to be a potent inhibitor that reduced biofilm formation of S.marcescens NJ01 by 79%.Scanning electron microscopy(SEM)and confocal laser scanning microscopy(CLSM)results revealed that treatment with m-NPe(50μg/ml)not only enhanced the susceptibility of the formed biofilms but also disrupted the architecture of biofilms by 84%.m-NPe(50μg/ml)decreased virulence factors in S.marcescens NJ01,reducing the activity of protease,prodigiosin,and extracellular polysaccharide(EPs)by 36%,72%,and 52%,respectively.In S.marcescens 4547,the activities of hemolysin and EPs were reduced by 28%and 40%,respectively,outperforming the positive control,vanillic acid(VAN).The study also found that the expression levels of QS-and biofilm-related genes(flhD,fimA,fimC,sodB,bsmB,pigA,pigC,and shlA)were downregulated by 1.21-to 2.32-fold.Molecular dynamics analysis showed that m-NPe could bind stably to SmaR,Rhll,RhiR,LasR,and CviR proteins in a 0.1 M sodium chloride solution.Importantly,a microscale thermophoresis(MST)test revealed that SmaR could be a target protein for the screening of a quorum sensing inhibitor(QSl)against S.marcescens.Overall,this study highlights the efficacy of m-NPe in suppressing the virulence factors of S.marcescens,identifying it as a new potential Qsl and antibiofilm agent capable of restoring or improving antimicrobial drug sensitivity.
文摘Serratia is an opportunistic pathogenic bacterium of the Enterobacteriaceae family, occasionally responsible for epidemics of nosocomial infections in critical departments;in particular in neonatal intensive care units Enterobacteriaceae form a large family of Gram-negative bacteria, which cause diseases of highly variable severity, due to distinct pathogenic mechanisms. This family is heterogeneous as it consists of about 30 genera of bacteria and more than 100 species. However, all these germs have in common their preferential location in the digestive system, some being part of the normal flora although they are also present in the environment. Several metabolic processes characterize this bacterial family. These include the ability to reduce nitrate to nitrite (for energy generation), ferment glucose, lack cytochrome oxidase, be aerobic or anaerobic, motile or immobile, … Enterobacteriaceae constitute more than 80% of the germs isolated in the laboratory: Escherichia, Salmonella, Shigella, Klebsiella, Enterobacter, Serratia, Proteus, Morganella and Yersinia are the rods most often found. Regarding nosocomial infection to Serratia, this bacterium colonizes the respiratory, digestive and urinary systems of patients, mainly responsible for bacteremia, infections of the lower respiratory tract, urinary and skin infections. Through our work, we report the epidemic experienced in the neonatal intensive care unit MOHAMMED VI university hospital, mother-child hospital MARRAKECH MOROCCO for three months from December at February 2023 interesting 30 newborns whose clinical presentation was different, the positive diagnosis was based on blood and geographical samples taken by the bacteriology department to isolate the offending germs, the therapeutic management of our patients consisted essentially of various hygiene measures in association with dual antibiotic therapy based on meropenem and amikacin. The evolution was marked by the death of 21 patients (70%) and the recovery of 9 patients (30%). The prognosis of infection by serratia remains bleak with high morbidity interest in prevention by respecting the rules of hygiene, which begins with hand disinfection.
文摘We present the 3<sup>rd</sup> known case of a Serratia rubidaea urinary tract infection in a 49-year-old male with past medical history of bilateral lower extremity paraplegia who presented to the emergency room with fever, lethargy, and red tinted urine. The Serratia genus, of the family Enterobacteracia, in particular the species marcescens, are important causes of infection in humans, animals, and insects, however, until the mid-1950s, this was not the case, and the organism was considered non-pathogenic and frequently used in medical experiments. Serratia are facultatively anaerobic, gram-negative rods, the majority with peritrichous flagella primarily inhabiting soil, water, and plant surfaces. Serratia marcescens is now a known pathogen, however, less frequently isolated species, including Serratia rubidaea, are worthy of discussion, especially due to its characteristic red color and rarity. We aim to increase the awareness Serratia rubidaea including its presentation, inherent antimicrobial resistance, and treatment options.
文摘从大豆土壤中分离纯化得到一株具有卵磷脂和乐果降解能力的菌株Yj1,对该菌株进行鉴定、生长条件优化、酶活性鉴定以及有机磷降解酶的分离纯化。结果表明,Yj1与Serratia marcescens WW4(CP003959.1)的16S r DNA相似度为99%。正交试验对所需培养基进行优化,得到该菌株的最佳生长条件为甘露糖、蛋白胨和p H 8的组合。Yj1菌株在两种磷源条件下,菌株生长量均很低,但72 h内以大豆卵磷脂为磷源时的菌体生长情况优于乐果。以大豆卵磷脂为磷源时酸性磷酸酶、碱性磷酸酶与有机磷降解酶活性明显高于以乐果为磷源时的酶活,且72 h内碱性磷酸酶活性一直都高于酸性磷酸酶和有机磷降解酶。硫酸铵沉淀法结合阳离子交换层析成功从Yj1菌体中分离纯化了有机磷降解酶,SDS-PAGE结果显示纯化的蛋白为单一条带。且阳离子交换层析的提纯倍数是硫酸氨沉淀的5.303倍,硫酸氨沉淀为粗酶的1.416倍。
基金supported by the grants from the National High Technology Research and Development Program of China (2011AA10A206)the China Agriculture Research System (CARS-02)the Science and Technology Support Program of Hebei, China (12220301D)
文摘A total of 479 bacterial strains were isolated from brine （Bohai, Qinhuangdao City, Hebei Province, China）. Bioassay results indicated that 4 strains named Hal, Hal7, Ha38, and Ha384 had herbicidal activity. And strain Hal had the highest effective herbicidal activity. As a result, this study aims to JdentJfy strain Hal, characterize its physiological and biological activities, evaluate the herbicidal activity of its metabolites, and develop a ＇pesta＇ formulation and assess its effectiveness on Digitaria sanguinalis. Hal was identified as Serratia marcescens based on 16S rDNA sequencing. This strain has a flagellum, a diameter of 0.5 to 0.8 IJm, and a length of 0.9 to 2.0 IJm. The indole test shows positive results, and the catalase enzyme exhibits strong positive reactions. Results further showed that the inhibitory concentration （IC50） of the crude extracts to D. sanguinalis radicula and coleoptile were 3.332 and 2.828 mg mL-1, respectively. Both the suppression of D. sanguinalis and the cell viability of the Hal formulation in ＇pesta＇ were higher when stored at 4℃ than at （25＋2）℃. These results indi- cated that S. marcescens Hal can potentially be used as a biocontrol agent against D. sanguinalis.
