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High quality repair of osteochondral defects in rats using the extracellular matrix of antler stem cells 被引量:1
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作者 Yu-Su Wang Wen-Hui Chu +4 位作者 Jing-Jie Zhai Wen-Ying Wang Zhong-Mei He Quan-Min Zhao Chun-Yi Li 《World Journal of Stem Cells》 SCIE 2024年第2期176-190,共15页
BACKGROUND Cartilage defects are some of the most common causes of arthritis.Cartilage lesions caused by inflammation,trauma or degenerative disease normally result in osteochondral defects.Previous studies have shown... BACKGROUND Cartilage defects are some of the most common causes of arthritis.Cartilage lesions caused by inflammation,trauma or degenerative disease normally result in osteochondral defects.Previous studies have shown that decellularized extracellular matrix(ECM)derived from autologous,allogenic,or xenogeneic mesenchymal stromal cells(MSCs)can effectively restore osteochondral integrity.AIM To determine whether the decellularized ECM of antler reserve mesenchymal cells(RMCs),a xenogeneic material from antler stem cells,is superior to the currently available treatments for osteochondral defects.METHODS We isolated the RMCs from a 60-d-old sika deer antler and cultured them in vitro to 70%confluence;50 mg/mL L-ascorbic acid was then added to the medium to stimulate ECM deposition.Decellularized sheets of adipocyte-derived MSCs(aMSCs)and antlerogenic periosteal cells(another type of antler stem cells)were used as the controls.Three weeks after ascorbic acid stimulation,the ECM sheets were harvested and applied to the osteochondral defects in rat knee joints.RESULTS The defects were successfully repaired by applying the ECM-sheets.The highest quality of repair was achieved in the RMC-ECM group both in vitro(including cell attachment and proliferation),and in vivo(including the simultaneous regeneration of well-vascularized subchondral bone and avascular articular hyaline cartilage integrated with surrounding native tissues).Notably,the antler-stem-cell-derived ECM(xenogeneic)performed better than the aMSC-ECM(allogenic),while the ECM of the active antler stem cells was superior to that of the quiescent antler stem cells.CONCLUSION Decellularized xenogeneic ECM derived from the antler stem cell,particularly the active form(RMC-ECM),can achieve high quality repair/reconstruction of osteochondral defects,suggesting that selection of decellularized ECM for such repair should be focused more on bioactivity rather than kinship. 展开更多
关键词 Osteochondral defect repair Mesenchymal stem cells extracellular matrix DECELLULARIZATION Antler stem cells Reserve mesenchymal cells Xenogeneic
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Effects of Shikonin on Proliferation, Apoptosis, and Extracellular Matrix of Human Mesangial Cells 被引量:2
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作者 李海涛 李晓冬 朱荃 《Journal of Nanjing Medical University》 2004年第6期304-307,共4页
Objective:To investigate the effect of shikonin on the proliferation, apoptosis and extracellular matrix (ECM) of human mesangial cells (MC). Methods: MC was cultured in vitro with different concentrations of glucose ... Objective:To investigate the effect of shikonin on the proliferation, apoptosis and extracellular matrix (ECM) of human mesangial cells (MC). Methods: MC was cultured in vitro with different concentrations of glucose (30, 50, 80 mmol/L). The cell growth was observed by using MTT method and apoptosis by using an aunexin-V-Fluos. Immunohistochemical studies for Laminin (LN), Fibronectin (FN) and type Ⅳ Collagens (Col Ⅳ) were measured. Results: Shikonin inhibited their growth (P<0.05) and apoptosis in the glycated cultured cells. Shikonin 0.05 mmol/L significantly reduced the secretion of LN, FN and Col Ⅳ from MC (P<0.05) cultured in 30, 50 and 80 mmol/L glucose. Conclusion: Shikonin could prevent or treat diabetic nephropathy (DN) and glomerulosclerosis (GS). 展开更多
关键词 SHIKONIN mesangial cell PROLIFERATION extracellular matrix APOPTOSIS GLOMERULOSCLEROSIS
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Cell viability and extracellular matrix synthesis in a co-culture system of corneal stromal cells and adipose-derived mesenchymal stem cells 被引量:3
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作者 Ting Shen Jiang Shen +4 位作者 Qing-Qing Zheng Qiu-Shi Li Hai-Lan Zhao Lei Cui Chao-Yang Hong 《International Journal of Ophthalmology(English edition)》 SCIE CAS 2017年第5期670-678,共9页
AIM:To investigate the impact of adipose-derived mesenchymal stem cells(ADSCs) on cell viability and extracellular matrix(ECM) synthesis of corneal stromal cells(CSCs). METHODS:ADSCs and CSCs were obtained fro... AIM:To investigate the impact of adipose-derived mesenchymal stem cells(ADSCs) on cell viability and extracellular matrix(ECM) synthesis of corneal stromal cells(CSCs). METHODS:ADSCs and CSCs were obtained from the corneas of New Zealand white rabbits and indirectly cocultured in vitro. The proliferative capacity of CSCs in the different groups was assessed by CCK-8 assays. Annexin V-fluorescein isothiocyanate(FITC)/proliferation indices(PI) assays were used to detect the apoptosis of CSCs. The expression levels of matrix metalloproteinase(MMP), such as MMP1, MMP2, MMP9, and collagens were also evaluated by Western blot. RESULTS:ADSCs significantly promoted proliferation and invasion of CSCs in the indirect co-culture assays. The co-cultural group displayed much higher ability of proliferation, especially under the co-culture conditions of ADSCs for 3d, compared with that CSCs cultured alone. The PI of CSCs in the co-culture system were increased approximately 3-8-fold compared with the control group. A significant change was observed in the proportions of cells at apoptosis(early and late) between the negative control group(6.34% and 2.06%) and the ADCSs-treated group(4.69% and 1.59%). The expression levels of MMPs were down regulated in the co-culture models. Compared with the control group, the decrease intensities of MMP-1, MMP-2 and MMP-9 in CSCs/ADSCs group were observed, 3.90-fold, 1.09-fold and 3.03-fold, respectively. However, the increase intensities of collagen type(I, II, III, IV, and V) in CSCs were observed in CSCs/ADSCs group, 3.47-fold,4.30-fold, 2.35-fold, 2.55-fold and 2.43-fold, respectively, compared to that in the control group. The expressions of aldehyde dehydrogenase and fibronectin in CSCs were upregulated in the co-culture models.CONCLUSION:ADSCs play a promotive role in CSCs' growth and invasion, which may be partially associated with MMPs decrease and collagens increase, resulting in a positive participation in the plasticity and ECM synthesis of CSCs. This provided a new insight into the extensive role of ADSCs in CSCs and a potential molecular target for corneal therapy. 展开更多
关键词 adipose-derived mesenchymal stem cell corneal stromal cells extracellular matrix PLASTICITY
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Effects of transforming growth factor β2 and connective tissue growth factor on induction of epithelial mesenchymal transition and extracellular matrix synthesis in human lens epithelial cells 被引量:7
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作者 Cheng Pei Bo Ma +2 位作者 Qian-Yan Kang Li Qin Li-Jun Cui 《International Journal of Ophthalmology(English edition)》 SCIE CAS 2013年第6期752-757,共6页
AIM:To Investigate the effects of transforming growth factorβ2(TGF-β2)and connective tissue growth factor(CTGF)on transdifferentiation of human lens epithelial cells(HLECs)cultured in vitro and synthesis of extracel... AIM:To Investigate the effects of transforming growth factorβ2(TGF-β2)and connective tissue growth factor(CTGF)on transdifferentiation of human lens epithelial cells(HLECs)cultured in vitro and synthesis of extracellular matrix(ECM).METHODS:HLECs were treated with TGF-β2(0,0.5,1.0,5,10μg/L)and CTGF(0,15,30,60,100μg/L)for different times(0,24,48,72h)in vitro and the expression ofα-smooth muscle actin(α-SMA),the main component of the extracellular matrix typeⅠcollagen(Col-1)and fibronectin(Fn)were measured by using real-time polymerase chain reaction(PCR)and western-blot.RESULTS:TGF-β2 and CTGF significantly increased expression ofα-SMA mRNA and protein(P【0.05,P【0.001),Fn mRNA and protein(P【0.001),Col-1 mRNA and protein(P【0.001).TGF-β2 could induce HLECs expression of CTGF mRNA and protein in dosedependent manner(P【0.05,P【0.001).TGF-β2 and CTGF could induce HLECs to expressα-SMA,Fn and Col-1 in time-dependent manner.Each time of TGF-β2and CTGF induced HELCs expression ofα-SMA,Fn,Col-1 mRNA and protein was significant increase compared with control(P【0.05,P【0.001).CONCLUSION:TGF-β2 and CTGF could induce HLECs epithelial mesenchymal transition and ECM synthesis. 展开更多
关键词 transforming growth factor β 2 connective tissue growth factor posterior capsular opacification human lens epithelial cells extracellular matrix α -smooth muscle actin type I collagen fibronectin
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Nectin-like Molecule 1 Inhibits the Migration and Invasion of U251 Glioma Cells by Regulating the Expression of An Extracellular Matrix Protein Osteopontin 被引量:2
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作者 Bin Yin Ke-han Li Tai An Tao Chen Xiao-zhong Peng 《Chinese Medical Sciences Journal》 CAS CSCD 2010年第2期100-104,共5页
Objective To investigate the molecular mechanism of nectin-like molecule 1(NECL1) inhibiting the migration and invasion of U251 glioma cells.Methods We infected U251 glioma cells with adeno-nectin-like molecule 1(Ad-N... Objective To investigate the molecular mechanism of nectin-like molecule 1(NECL1) inhibiting the migration and invasion of U251 glioma cells.Methods We infected U251 glioma cells with adeno-nectin-like molecule 1(Ad-NECL1) or empty adenovirus(Ad).Transwell and wound healing assays were performed to observe the migration of U251 cells incubated with the cell supernatant from Ad-NECL1 or Ad infected U251 cells.DNA microarray was applied to screen the gene expression profile after the restoration of NECL1 in U251 glioma cell lines.The differential expression of osteopontin(OPN),a gene related to migration and invasion,was further analyzed with semi-quantitative reverse transcription-polymerase chain reaction(RT-PCR),Western blot,and immunohistochemistry.Results The restoration of NECL1 inhibited migration of U251 cells significantly(P<0.05).Altogether 195 genes were found differentially expressed by microarray,in which 175 were up-regulated and 20 down-regulated,including 9 extracellular matrix proteins involved in the migration of cells.Both mRNA and protein expressions of OPN,the most markedly reduced extracellular matrix protein,were found decreased in U251 cells after restoration of NECL1.Immunohistochemical assay also detected an increase of OPN in glioma tissues,related with the progressing of malignant grade.Conclusion A link might exist between NECL1 and the extracellular matrix protein OPN in inhibiting the migration and invasion of U251 glioma cells. 展开更多
关键词 nectin-like molecule 1 glioma cell line extracellular matrix protein OSTEOPONTIN
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Nanofibrous Scaffold Containing Osteoblast-Derived Extracellular Matrix for the Proliferation of Bone Marrow Mesenchymal Stem Cells 被引量:1
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作者 吴云亮 秦春萍 +3 位作者 余哲泡 王先流 张彦中 娄向新 《Journal of Donghua University(English Edition)》 EI CAS 2017年第6期756-760,共5页
Extracellular matrix( ECM) plays a prominent role in establishing and maintaining an appropriate microenvironment for tissue regeneration. The aims of this study were to construct a tissue engineered scaffold by recon... Extracellular matrix( ECM) plays a prominent role in establishing and maintaining an appropriate microenvironment for tissue regeneration. The aims of this study were to construct a tissue engineered scaffold by reconstituting osteoblast cell-derived ECM( O-ECM) on the electrospun nanofibrous scaffold,and further to evaluate its subsequent application for promoting the proliferation of bone marrow mesenchymal stem cells( BMSCs). To engineer a biomimetic scaffold, calvarial osteoblasts and electrospun poly-llactic acid( PLLA) nanofibers were prepared and subjected to decellularize for O-ECM deposition. To evaluate and characterize the O-ECM/PLLA scaffold, the morphology was examined and several specific mark proteins of osteoblasts matrix were evaluated.Furthermore,the cell counting kit-8( CCK-8) assay was used to detect the proliferation of the BMSCs cultivated on the O-ECM/PLLA scaffold. The results indicated O-ECM/PLLA scaffold was loaded with Collagen I, Fibronectin, and Laminin, as the composition of the marrow ECM. After decellularization,O-ECM deposition was observed in O-ECM/PLLA scaffold. Moreover,the O-ECM/PLLA scaffold could significantly enhance the proliferation of BMSCs,suggesting better cytocompatibility compared to the other groups tested. Taken together,a biomimetic scaffold based on the joint use of O-ECM and PLLA biomaterials,which represents a promising approach to bone tissue engineering, facilitates the expansion of BMSCs in vitro. 展开更多
关键词 tissue engineering extracellular matrix(ECM) electrospun nanofibers bone marrow mesenchymal stem cells(BMSCs)
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Dendritic cells and the extracellular matrix:A challenge for maintaining tolerance/homeostasis 被引量:1
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作者 Sucharita P Shankar May Griffith +1 位作者 John V Forrester Lucia Kuffová 《World Journal of Immunology》 2015年第3期113-130,共18页
The importance of the extracellular matrix (ECM) in contributing to structural, mechanical, functional and tissue-specific features in the body is well appreciated. While the ECM was previously considered to be a pa... The importance of the extracellular matrix (ECM) in contributing to structural, mechanical, functional and tissue-specific features in the body is well appreciated. While the ECM was previously considered to be a passive bystander, it is now evident that it plays active, dynamic and fexible roles in shaping cell survival, differentiation, migration and death to varying extents depending on the specific site in the body. Dendritic cells (DCs) are recognized as potent antigen presenting cells present in many tissues and in blood, continuously scrutinizing the microenvironment for antigens and mounting local and systemic host responses against harmful agents. DCs also play pivotal roles in maintaining homeostasis to harmless self-antigens, critical for preventing autoimmunity. What is less understood are the complex interactions between DCs and the ECM in maintaining this balance between steady-state tissue residence and DC activation during inflammation. DCs are finely tuned to inflammation-induced variations in fragment length, accessible epitopes and post-translational modifications of individual ECM components and correspondingly interpret these changes appropriately by adjusting their profiles of cognate binding receptors and downstream immune activation. The successful design and composition of novel ECM-based mimetics in regenerative medicine and other applications rely on our improved understanding of DC-ECM interplay in homeostasis and the challenges involved in maintaining it. 展开更多
关键词 Dendritic cells extracellular matrix TOLERANCE BIOMATERIALS HOMEOSTASIS Regenerative medicine Biointeractive implants
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Activation of extracellular signal-related kinases 1 and 2 in Sertoli cells in experimentally cryptorchid rhesus monkeys 被引量:6
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作者 Xue-Sen Zhang Zhi-Hong Zhang Shu-Hua Guo Wei Yang Zhu-Qiang Zhang Jin-Xiang Yuan Xuan Jin Zhao-Yuan Hu Yi-Xun Liu 《Asian Journal of Andrology》 SCIE CAS CSCD 2006年第3期265-272,共8页
Aim: To assess the spatiotemporal changes in the expression of extracellular signal-regulated kinases 1 and 2 (ERK1/ 2), c-Jun N-terminal kinases (JNK) and p38 mitogen-activated protein kinases (MAPK) in respon... Aim: To assess the spatiotemporal changes in the expression of extracellular signal-regulated kinases 1 and 2 (ERK1/ 2), c-Jun N-terminal kinases (JNK) and p38 mitogen-activated protein kinases (MAPK) in response to heat stress in the cryptorchid testis, and to investigate a possible relation to Sertoli cell dedifferentiation. Methods: Immunohistochemistry and western blot were used to examine the expression and activation of ERK1/2, p38 and JNK in the cryptorchid testis at various stages after experimental cryptorchidism. Results: The abdominal temperature did not obviously change the total ERK1/2 expression but significantly activated phospho-ERK1/2 in the Sertoli cells of the cryptorchid testis. Heat stress increased total JNK expression in the Sertoli cells of the cryptorchid testis but did not activate phospho-JNK. Neither total p38 nor phospho-p38 was induced by heat stress in the Sertoli cells of the cryptorchid testis. Changes in the spatiotemporal expression of cytokeratin 18 (CK18), a marker of immature or undifferentiated Sertoli cells, were induced in the cryptorchid testis in a pattern similar to the activation of ERK1/2. Condusion: The activation of ERK1/2 in the testis may be related to dedifferentiation of Sertoli cells under heat stress induced by experimental cryptorchidism. 展开更多
关键词 rhesus monkey CRYPTORCHIDISM sertoli cell DEDIFFERENTIATION extracellular signal-regulated kinases 1 and 2
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Neural differentiation from pluripotent stem cells:The role of natural and synthetic extracellular matrix
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作者 Yan Li Meimei Liu +1 位作者 Yuanwei Yan Shang-Tian Yang 《World Journal of Stem Cells》 SCIE CAS 2014年第1期11-23,共13页
Neural cells differentiated from pluripotent stem cells(PSCs), including both embryonic stem cells and induced pluripotent stem cells, provide a powerful tool for drug screening, disease modeling and regenerative medi... Neural cells differentiated from pluripotent stem cells(PSCs), including both embryonic stem cells and induced pluripotent stem cells, provide a powerful tool for drug screening, disease modeling and regenerative medicine. High-purity oligodendrocyte progenitor cells(OPCs) and neural progenitor cells(NPCs) have been derived from PSCs recently due to the advancements in understanding the developmental signaling pathways. Extracellular matrices(ECM) have been shown to play important roles in regulating the survival, proliferation, and differentiation of neural cells. To improve the function and maturation of the derived neural cells from PSCs, understanding the effects of ECM over the course of neural differentiation of PSCs is critical. During neural differentiation of PSCs, the cells are sensitive to the properties of natural or synthetic ECMs, including biochemical composition, biomechanical properties, and structural/topographical features. This review summarizes recent advances in neural differentiation of humanPSCs into OPCs and NPCs, focusing on the role of ECM in modulating the composition and function of the differentiated cells. Especially, the importance of using three-dimensional ECM scaffolds to simulate the in vivo microenvironment for neural differentiation of PSCs is highlighted. Future perspectives including the immediate applications of PSC-derived neural cells in drug screening and disease modeling are also discussed. 展开更多
关键词 PLURIPOTENT stem cells Neural DIFFERENTIATION extracellular matrix Three-dimensional DRUG screening
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Effects of extracellular matrix proteins on expansion, proliferation and insulin-producing-cell differentiation of ARIP cells
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作者 Gary G. Adams Yu-Xin Cui 《Journal of Biomedical Science and Engineering》 2009年第4期216-226,共11页
Regeneration of transplantable pancreatic islet cells has been considered to be a promising alternative therapy for type 1 diabetes. Re-search has suggested that adult pancreatic stem and progenitor cells can be deriv... Regeneration of transplantable pancreatic islet cells has been considered to be a promising alternative therapy for type 1 diabetes. Re-search has suggested that adult pancreatic stem and progenitor cells can be derived into insulin-producing cells or cultivated islet-like clusters given appropriate stimulating condi- tions. In this study we explored the effect of selective extracellular matrix (ECM) proteins on the potential of insulin-producing cell differen-tiation using ARIP cells, an adult rat pancreatic ductal epithelial cell line, as a model in vitro. Quantitative single cell morphology analysis indicated that all the four ECM proteins we have used (type I collagen, laminin, fibronectin and vitronectin) increased the single cell area and diameter of ARIP cells. In addition, se-rum-free cell cultivation was dependent on cell density and particular components;and serum could be replaced when systematic optimisa-tion could be performed. Surface treated with laminin was shown to be able to enhance overall cell expansion in the presence of de-fined serum-free medium conditions. Collagen treated surfaces enhanced insulin production in the presence of GLP-1 although the insulin gene expression was however weak accord-ingly. Our results suggest that selective ECM proteins have effects on single cell morphol-ogy, adhesion and proliferation of ARIP cells. These ECM molecules however do not have a potent effect on the insulin-producing cell dif-ferentiation potential of ARIP cells even com-bining with GLP-1. 展开更多
关键词 extracellular matrix PROLIFERATION Differentiation ARIP cells INCRETIN GLP-1
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Activation of extracellular signal-related kinases 1 and 2 in Sertoli cells in experimentally cryptorchid rhesus monkeys 被引量:1
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作者 Xue-Sen Zhang~+ Zhi-Hong Zhang~+ Shu-Hua Guo Wei Yang,Zhu-Qiang Zhang Jin-Xiang Yuan Xuan Jin Zhao-Yuan Hu Yi-Xun Liu State Key Laboratory of Reproductive Biology,Institute of Zoology,Chinese Academy of Sciences,25 Bei Si Huan Road West,Beijing 100081,China 《Asian Journal of Andrology》 SCIE CAS CSCD 2006年第A03期265-272,385,共5页
Aim:To assess the spatiotemporal changes in the expression of extracellular signal-regulated kinases 1 and 2(ERK1/ 2),c-Jun N-terminal kinases(JNK)and p38 mitogen-activated protein kinases(MAPK)in response to heat str... Aim:To assess the spatiotemporal changes in the expression of extracellular signal-regulated kinases 1 and 2(ERK1/ 2),c-Jun N-terminal kinases(JNK)and p38 mitogen-activated protein kinases(MAPK)in response to heat stress in the cryptorchid testis,and to investigate a possible relation to Sertoli cell dedifferentiation.Methods:Immunohis- tochemistry and western blot were used to examine the expression and activation of ERK1/2,p38 and JNK in the cryptorchid testis at various stages after experimental cryptorchidism.Results:The abdominal temperature did not obviously change the total ERK1/2 expression but significantly activated phospho-ERK1/2 in the Sertoli cells of the cryptorchid testis.Heat stress increased total JNK expression in the Sertoli cells of the cryptorchid testis but did not activate phospho-JNK.Neither total p38 nor phospho-p38 was induced by heat stress in the Sertoli cells of the cryptorchid testis.Changes in the spatiotemporal expression of cytokeratin 18(CK18),a marker of immature or undifferentiated Sertoli cells,were induced in the cryptorchid testis in a pattern similar to the activation of ERK1/2. Conclusion:The activation of ERK1/2 in the testis may be related to dedifferentiation of Sertoli cells under heat stress induced by experimental cryptorchidism. 展开更多
关键词 rhesus monkey CRYPTORCHIDISM sertoli cell DEDIFFERENTIATION extracellular signal-regulated kinases 1 and 2
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Dynamic culture of a thermosensitive collagen hydrogel as an extracellular matrix improves the construction of tissue-engineered peripheral nerve 被引量:3
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作者 Lanfeng Huang Rui Li +5 位作者 Wanguo Liu Jin Dai Zhenwu Du Xiaonan Wang Jianchao Ma Jinsong Zhao 《Neural Regeneration Research》 SCIE CAS CSCD 2014年第14期1371-1378,共8页
Tissue engineering technologies offer new treatment strategies for the repair of peripheral nerve injury, hut cell loss between seeding and adhesion to the scaffold remains inevitable. A thermosensitive collagen hydro... Tissue engineering technologies offer new treatment strategies for the repair of peripheral nerve injury, hut cell loss between seeding and adhesion to the scaffold remains inevitable. A thermosensitive collagen hydrogel was used as an extracellular matrix in this study and combined with bone marrow mesenchymal stem cells to construct tissue-engineered peripheral nerve composites in vitro. Dynamic culture was performed at an oscillating frequency of 0.5 Hz and 35° swing angle above and below the horizontal plane. The results demonstrated that bone marrow mesenchymal stem cells formed membrane-like structures around the poly-L-lactic acid scaffolds and exhibited regular alignment on the composite surface. Collagen was used to fill in the pores, and seeded cells adhered onto the poly-L-lactic acid fibers. The DNA content of the bone marrow mesenchymal stem cells was higher in the composites constructed with a thermosensitive collagen hydrogel compared with that in collagen I scaffold controls. The cellular DNA content was also higher in the thermosensitive collagen hydrogel composites constructed with the thermosensitive collagen hydrogel in dynamic culture than that in static culture. These results indicate that tissue-engineered composites formed with thermosensitive collagen hydrogel in dynamic culture can maintain larger numbers of seeded cells by avoiding cell loss during the initial adhe-sion stage. Moreover, seeded cells were distributed throughout the material. 展开更多
关键词 nerve regeneration peripheral nerve biomaterials extracellular matrix TISSUEENGINEERING nerve scaffold bone marrow mesenchymal stem cells thermosensitive collagen hydrogel poly-L-lactic acid dynamic culture NSFC grant neural regeneration
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TSP-1 promotes glomerular mesangial cell proliferation and extracellular matrix secretion in Thy-1 nephritis rats 被引量:2
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作者 Wen Qiu Yan Li +5 位作者 Jianbo Zhou Chenhui Zhao Jing Zhang Kai Shan Dan Zha Yingwei Wang 《The Journal of Biomedical Research》 CAS 2011年第6期402-410,共9页
The proliferation of glomerular mesangial cells (GMC) and secretion of the extracellular matrix (ECM) in rat with Thy-1 nephritis (Thy-lN) resembling human mesangioproliferative glomerulonephritis have been expl... The proliferation of glomerular mesangial cells (GMC) and secretion of the extracellular matrix (ECM) in rat with Thy-1 nephritis (Thy-lN) resembling human mesangioproliferative glomerulonephritis have been explored for many years; however, the molecular mechanisms of GMC proliferation and ECM production remain unclear. Our previous studies have demonstrated that the thrombospondin-1 (TSP-1) gene was involved in mediating rat GMC proliferation and ECM synthesis induced by sublytic C5b-9 in vitro. 111 the present study, the roles of the TSP-1 gene in GMC proliferation, ECM production, and urinary protein secretion in Thy-lN rats were determined by using TSP-1 small hairpin RNA, and the results revealed that silencing of the TSP-1 gene in rat renal tissues could diminish GMC proliferation (P 〈 0.01) and ECM secretion (P 〈 0.01) as well as urinary protein secretion (P 〈 0.05) in Thy-lN rats. Together, the current findings suggested that TSP-1 gene expression was required for GMC proliferation and ECM production in Thy-lN rats. 展开更多
关键词 Thy-1 nephritis glomerular mesangial cells (GMCs) PROLIFERATION extracellular matrix thrombospondin-1 TSP-1)
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Neuronal growth cones and regeneration: gridlock within th extracellular matrix
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作者 Diane M.Snow 《Neural Regeneration Research》 SCIE CAS CSCD 2014年第4期341-342,共2页
The extracellular matrix is a diverse composition of glycoproteins and proteoglycans found in all cellular systems. The extracellular matrix, abundant in the mammalian central nervous system, is temporally and spatial... The extracellular matrix is a diverse composition of glycoproteins and proteoglycans found in all cellular systems. The extracellular matrix, abundant in the mammalian central nervous system, is temporally and spatially regulated and is a dynamic "living" entity that is reshaped and redesigned on a continuous basis in response to changing needs. Some modifications are adaptive and some are maladaptive. It is the maladaptive responses that pose a significant threat to successful axonal regeneration and/or sprouting following traumatic and spinal cord injuries, and has been the focus of a myriad of research laboratories for many years. This review focuses largely on the extracellular matrix component, chondroitin sulfate proteoglycans, with certain comparisons to heparan sulfate proteoglycans, which tend to serve opposite functions in the central nervous system. Although about equally as well characterized as some of the other proteoglycans such as hyaluronan and dermatan sulfate proteoglycan, chondroitin sulfate proteoglycans are the most widely researched and discussed proteoglycans in the field of axonal injury and regeneration. Four laboratories discuss various aspects of chondroitin sulfate proteoglycans and proteoglycans in general with respect to their structure and function (Beller and Snow), the recent discovery of specific chondroitin sulfate proteoglycan receptors and what this may mean the field (Shen), extracellular for increased advancements in matrix degradation by matrix metalloproteinases, which sculpt and resculpt to provide support for outgrowth, synapse formation, and synapse stability (Phillips et al.), and the perilesion microenvironment with respect to immune system function in response to proteoglycans and central nervous system injuries (Jakeman et al.). 展开更多
关键词 cell CSPG gridlock within th extracellular matrix ECM
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Immuohistochemical study on smooth muscle cell proliferation, pheno-typic modulation, and extracellular matrix accumulation in venous arterial grafts in rabbits
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作者 张卫达 朱海龙 《Journal of Medical Colleges of PLA(China)》 CAS 2002年第2期120-124,共5页
Objective: To study the kinetics and distribution of smooth muscle cell (SMC) proliferation, phe-notypic modulation, and various extracellular matrix (ECM) components accumulation during vein graft remodeling. Methods... Objective: To study the kinetics and distribution of smooth muscle cell (SMC) proliferation, phe-notypic modulation, and various extracellular matrix (ECM) components accumulation during vein graft remodeling. Methods: Normal vein and vein graft in carotid arteries were examined on d 4, d 7, d 14, d 60 and d180 after bypass grafting with immunohistochemical markers of cellular proliferation (proliferating cell nuclear antigen, PCNA), cytoskeletal protein production (a-actin SMC), myosin heavy chain (MHO iso-forms, ECM proteins, and histochemistry (hematoxylin eosin and Elastica-van Gieson stain). Results: Normal veins demonstrated an extremely low level of cellular proliferation and expressed as adult phenotype SM-Cs in media. After bypass grafting, medial SMCs in the graft appeared to be damaged and began to proliferate on d 4, and subsequently migrated and formed the neointima on d 7. Thereafter, the neointima thickened throughout the 180-day period of the experiment, although the neointimal SMC proliferation decreased after d 14. Meanwhile SMCs underwent a distinct phenotypic change from normal adult type to embryonic type. On d 60, embryonic phenotype SMCs began to return to the adult phenotype, but remain to be present in the neointima for as long as 180 d. ECM components including type I collagen, heparin sulfate proteoglucan (HSPG), and dermatan sulfate proteoglcan (decorin) were detected within the neointima on d 7. Thereafter, the accumulation of ECM increased progressively with time. On d 180, a large amount of ECM components were found in the neointima. HSPG mainly accumulated in the superficial and cellular region of the neointima , decorin, on other hand, located in hypocellular area deep in neointima. Type I collagen scatted in both regions. The elastic fibers became rich and arranged continuously in the neointima. Conclusion: The neointima of vein graft was initially formed by proliferation of the embryonic-type SMCs and then thickened infinitely due to ECM accumulation. Prolonged existence of the embryonic-type SMCs in the neointima may contribute to ECM accumulation and increase in the neointima thickness infinitely, which may predispose accelerated stenosis in the vein graft. 展开更多
关键词 venous arterial grafts t smooth muscle cell extracellular matrix REMODELING
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NEGATIVE REGULATORY ROLE OF SOMATOSTATIN AND OCTREOTIDE IN EXTRACELLULAR MATRIXES METABOLISM OF RAT HEPATIC STELLATE CELL
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作者 潘勤 李定国 +2 位作者 陆汉明 张文竹 徐芹芳 《Journal of Shanghai Second Medical University(Foreign Language Edition)》 2008年第2期71-76,共6页
Objective To investigate the regulatory effects of somatostatin (SST) and octreotide (OCT) on the extracellular matrixes (ECM) metabolism of rat hepatic stellate cells (HSCs). Methods Being treated with different conc... Objective To investigate the regulatory effects of somatostatin (SST) and octreotide (OCT) on the extracellular matrixes (ECM) metabolism of rat hepatic stellate cells (HSCs). Methods Being treated with different concentrations of SST or OCT, the mRNA levels of collage type I, III and the expression of collagen, matrix metalloproteinase-1(MMP-1), tissue inhibitor of metalloproteinase-1 (TIMP-1) in activated HSCs were assessed by in situ hybridization (ISH), 3H-proline incorporation and immunocytochemistry, respectively. In addition, levels of hyaluronic acid (HA), laminin (LM), and procollagen type III (PCIII) in the culture supernatant of HSCs were also detected by means of enzyme-linked immunosorbent assay (ELISA). ResultsBoth SST and OCT markedly down-regulated the transcription of collagen type I, III in activated HSCs within the range of 10-6-10-7 mol/L and 10-5-10-7 mol/L, respectively (P<0.05). SST-treated groups of 10-6-10-7 mol/L and OCT-treated groups of 10-5-10-7 mol/L also demonstrated statistically decreased production of collagen, HA, LM, PCIII in a dose-dependent way (P<0.05). Furthermore, HSCs cocultured with SST (10-6 mol/L) or OCT (10-5-10-6 mol/L) exhibited TIMP-1 levels much lower than that of normal control (P<0.05), which resulted in an elevated ratio of MMP/TIMP. Conclusion SST and its analog may play a negative regulatory role in the ECM metabolism of HSCs. Their effect may be benefit for prevention and therapy of hepatic fibrosis. 展开更多
关键词 somatostatin octreotide extracellular matrix hepatic stellate cell
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Extracellular matrix and biomimetic engineering microenvironment for neuronal differentiation 被引量:4
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作者 Deepak Jain Sabrina Mattiassi +1 位作者 Eyleen L.Goh Evelyn K.F.Yim 《Neural Regeneration Research》 SCIE CAS CSCD 2020年第4期573-585,共13页
Extracellular matrix(ECM)influences cell differentiation through its structural and biochemical properties.In nervous system,neuronal behavior is influenced by these ECMs structures which are present in a meshwork,fib... Extracellular matrix(ECM)influences cell differentiation through its structural and biochemical properties.In nervous system,neuronal behavior is influenced by these ECMs structures which are present in a meshwork,fibrous,or tubular forms encompassing specific molecular compositions.In addition to contact guidance,ECM composition and structures also exert its effect on neuronal differentiation.This short report reviewed the native ECM structure and composition in central nervous system and peripheral nervous system,and their impact on neural regeneration and neuronal differentiation.Using topographies,stem cells have been differentiated to neurons.Further,focussing on engineered biomimicking topographies,we highlighted the role of anisotropic topographies in stem cell differentiation to neurons and its recent temporal application for efficient neuronal differentiation. 展开更多
关键词 BIOMIMETIC platforms biophysical cues contact guidance extracellular matrix NEURONAL development NEURAL regeneration NEURAL STEM CELL niche NEURONAL differentiation NEURONAL maturation STEM CELL topography
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Surface Topography Modulates Cell Mechanosensing of Extracellular Matrix
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作者 Bianxiao Cui Xiao Li +3 位作者 Wei Zhang Lasse Hyldgaard Klausen Francesca Santoro Zhao Wenting 《医用生物力学》 EI CAS CSCD 北大核心 2019年第A01期36-36,共1页
The interaction between the cell membrane and the extracellular matrix is crucial for many cellular functions by modulating mechanosensitive signaling pathways.Physical properties of the extracellular matrix such as s... The interaction between the cell membrane and the extracellular matrix is crucial for many cellular functions by modulating mechanosensitive signaling pathways.Physical properties of the extracellular matrix such as stiffness and topography affect such interactions.Our recent work reveals that surface topography of tens to hundreds of nanometer scale modulates cell signaling by activating intracellular curvature-sensitive proteins.We use vertical nanostructures protruding from a flat surface as a platform to induce precise curvatures on the cell membrane and to probe biological processes in live cells.Vertical nanopillars deform the plasma membrane inwards and induce membrane curvature when the cell engulfs them,leading to a reduction of the membrane-substrate gap distance.We found that the high membrane curvature induced by vertical nanopillars significantly affects the distribution of curvature-sensitive proteins and stimulates several cellular processes in live cells including cellular endocytosis and cytoskeleton dynamics.Our studies show a strong interplay between biological cells and nano-featured surfaces,which is an essential consideration for future development of interfacing devices. 展开更多
关键词 Surface TOPOGRAPHY Modulates CELL MECHANOSENSING of extracellular matrix
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Homogenized Porcine Extracellular Matrix Derived Injectable Tissue Construct with Gold Nanoparticles for Musculoskeletal Tissue Engineering Applications
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作者 Sarah E. Smith Colten L. Snider +5 位作者 David R. Gilley Daniel N. Grant Seth L. Sherman Bret D. Ulery David A. Grant Sheila A. Grant 《Journal of Biomaterials and Nanobiotechnology》 2017年第2期125-143,共19页
A unique porcine extracellular matrix (ECM) derived injectable tissue construct with 100 nm or 20 nm gold nanoparticles (AuNP) was developed for musculoskeletal tissue engineering applications. ECM has been shown to e... A unique porcine extracellular matrix (ECM) derived injectable tissue construct with 100 nm or 20 nm gold nanoparticles (AuNP) was developed for musculoskeletal tissue engineering applications. ECM has been shown to encourage cellularity and tissue remodeling due to its release of growth factors while AuNP have been shown to reduce reactive oxygen species (ROS) levels. Injectable tissue constructs were created by homogenizing decellularized porcine diaphragm tendon conjugated with 100 nm or 20 nm AuNP at 1x, 4x, and 8x concentrations. Extrusion force testing demonstrated that homogenized tissue constructs were injectable at an appropriate cannula size and force. L-929 murine fibroblasts were used to measure cell viability, cell proliferation, intracellular ROS levels, and cell migration in response to constructs. Enhanced cell viability and proliferation are observed on 1 × 20 nm AuNP constructs. ROS assays demonstrate reduced cellular ROS concentrations from all 20 nm AuNP constructs and from 8 × 100 nm AuNP constructs compared with constructs without nanoparticles. Cellular migration is higher towards 4 × 20 nm AuNP constructs compared with constructs without nanoparticles. Results support the potential use of a porcine ECM derived injectable tissue construct with AuNP as an injectable tissue construct to reduce inflammation and to promote tissue remodeling in musculoskeletal tissue engineering applications. 展开更多
关键词 extracellular matrix Gold Nanoparticles Homogenization CELL VIABILITY CELL Migration
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Extracellular matrix gel is necessary for in vitro cultivation of insulin producing cells from human umbilical cord blood derived mesenchymal stem cells 被引量:31
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作者 GAO Feng WU De-quan HU Yan-hua JIN Guang-xin 《Chinese Medical Journal》 SCIE CAS CSCD 2008年第9期811-818,共8页
Background Pancreatic islet cell transplantation is an effective approach to treat type 1 diabetes. However, this therapy is not widely used because of the severe shortage of transplantable donor islets. This study in... Background Pancreatic islet cell transplantation is an effective approach to treat type 1 diabetes. However, this therapy is not widely used because of the severe shortage of transplantable donor islets. This study investigated whether mesenchymal stem cells (MSCs) derived from human umbilical cord blood (UCB) could be transdifferentiated into insulin producing cells in vitro and the role of extracellular matrix (ECM) gel in this procedure. Methods Human UCB samples were collected and MSCs were isolated. MSCs specific marker proteins were analyzed by a flow cytometer, The capacities of osteoblast and adipocyte to differentiate were tested. Differentiation into islet like cell was induced by a 15-day protocol with or without ECM gel. Pancreatic characteristics were evaluated with immunofluorescence, reverse transcription polymerase chain reaction (RT-PCR) and flow cytometry. Insulin content and release in response to glucose stimulation were detected with chemiluminescent immunoassay system. Results Sixteen MSCs were isolated from 42 term human UCB units (38%). Human UCB-MSCs expressed MSCs specific markers and could be induced in vitro into osteoblast and adipocyte. Islet like cell clusters appeared about 9 days after pancreatic differentiation in the inducing system with ECM gel. The insulin positive cells accounted for (25.2~3.4)% of the induced cells. The induced cells expressed islet related genes and hormones, but were not very responsive to glucose challenge. When MSCs were induced without ECM gel, clusters formation and secretion of functional islet proteins could not be observed, Conclusions Human UCB-MSCs can differentiate into islet like cells in vitro and ECM gel plays an important role in pancreatic endocrine cell maturation and formation of three dimensional structures. 展开更多
关键词 umbilical cord blood mesenchymal stem cells induction insulin producing cells extracellular matrix
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