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Relationship and significance between anti-b2-glycoproteinⅠantibodies and platelet activation state in patients with ulcerative colitis 被引量:1
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作者 Yan-Hang Gao Pu-Jun Gao +2 位作者 Chun-Guang Wang Xiao-Cong Wang Yun-Feng Piao 《World Journal of Gastroenterology》 SCIE CAS CSCD 2008年第5期771-775,共5页
AIM: To study the relationship between anti-β2- glycoprotein Ⅰ (aβ2GPⅠ) antibodies and platelet activation state in patients with ulcerative colitis (UC) and its significance. METHODS: Peripheral blood sampl... AIM: To study the relationship between anti-β2- glycoprotein Ⅰ (aβ2GPⅠ) antibodies and platelet activation state in patients with ulcerative colitis (UC) and its significance. METHODS: Peripheral blood samples were collected from 56 UC patients (34 males and 22 females, aged 43.5 years, range 21-66 years), including 36 at active stage and 20 at remission stage, and 25 sex-and age-matched controls. The level of aβ2GP Ⅰ was measured by ELISA. The platelet activation markers, platelet activation complex- Ⅰ (PAC- Ⅰ ) and P-selectin (CD62P) were detected by flow cytometry. RESULTS: The A value for IgG aβ2GP Ⅰ in the active UC group was 0.61 ± 0.13, significantly higher than that in the remittent UC and control groups (0.50 ± 0.13 and 0.22 ± 0.14, P 〈 0.01). There was a significant difference between the two groups (P 〈 0.01). The A value for IgM aβ2GP Ⅰ in the active and remittent UC groups was 0.43 ± 0.13 and 0.38 ± 0.12, significantly higher than that in the control group (0.20 ± 0.12, P 〈 0.01). However, there was no significant difference between the two groups (P 〉 0.05). The PAC- Ⅰ positive rate for the active and remittent UC groups was 30.6% ± 7.6% and 19.6% ± 7.8% respectively, significantly higher than that for the control group (6.3% ± 1.7%,P 〈 0.01). There was a significant difference between the two groups (P 〈 0.01). The CD62P positive rate for the active and remittent UC groups was 45.0% ± 8.8% and 31.9% ± 7.8% respectively, significantly higher than that for the control group (9.2% ± 2.7%, P 〈 0.01). There was a significant difference between the two groups (P 〈 0.01). In the active UC group, the more severe the state of illness was, the higher the A value for IgG aβ2GP Ⅰ was, and the positive rate for PAC-Ⅰ and CD62P was positively correlated with the state of illness (Faβ2GP Ⅰ = 3.679, P 〈 0.05; FPAC-Ⅰ (%) = 5.346, P 〈 0.01; and FCD62P (%) = 5. 418, P 〈 0.01). Meanwhile, in the same state of illness, the A value for IgG aβ2GP Ⅰ was positively correlated to the positive rates for PAC-Ⅰ and CD62P. CONCLUSION: aβ2GP Ⅰ level, platelet activation state and their relationship of them are closely correlated with the pathogenesis and development of UC. 展开更多
关键词 β2-glycoprotein anti-β2-glycoprotein antibodies Ulcerative colitis Platelet activation HYPERCOAGULATION
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PGE<sub>2</sub>Generation in Myocardium from Isolated Rat Atrium under Hypoxia and Reoxygenation Conditions. Effect of Anti-<i>β</i><sub>1</sub>IgG from Patients with Chronic Severe Periodontitis
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作者 Sabrina Ganzinelli Silvia Reina +3 位作者 Mirian Matoso Germán González Celina Morales Enri Borda 《Pharmacology & Pharmacy》 2014年第2期204-215,共12页
Background: Hypoxia is one of the most frequently encountered stresses in health and disease. Methods: We compared the effects of an anti-β1 periodontal IgG (pIgG) and an authentic β1 adrenergic agonist, xamoterol, ... Background: Hypoxia is one of the most frequently encountered stresses in health and disease. Methods: We compared the effects of an anti-β1 periodontal IgG (pIgG) and an authentic β1 adrenergic agonist, xamoterol, on isolated myocardium from rat atria contractility. We used an ELISA assay to measure the generation of PGE2 in vitro after the addition of either the antibody or the adrenergic agonist. We analyzed the myocardium histopathologically in the presence of both the antibody and/or the adrenergic agonist drug during normoxia, hypoxia and reperfusion conditions. Results: PGE2 generation increased during the hypoxia and was unchanged during reoxygenation period compared with the production of this prostanoid in atria during normoxia condition. A β1 specific adrenoceptor antagonist atenolol and the β1 synthetic peptide abrogated the increment of the prostanoid in the presence of pIgG but only atenolol due to it in the presence of xamoterol. The increment of PGE2 was dependent on the activation of cox-1 and cox-2 isoforms. Moreover, cox-2 was more active and produced more increments in the production of PGE2 in the presence of the pIgG than cox-1 activation. Histopathologically, studies of myocardium specimens during these different periods of the experimental protocol: basal (B), hypoxia (H) and reoxygenation (R), were also performed and showed tissue necrosis and edematization at the myocardium level. Conclusion: The phenomenon studied here supports the notion that PGE2 may be responsible for tissue edematization. PGE2 maybe acts as a beneficial modulator in the myocardium and prevents a major injury of it. The inflammation damage to the heart organ and cardiomyocytes caused by the actions of the antibodies in the course of heart lesions provoked by cardiovascular autoimmune disease, explains some of these results obtained in the present experiments. Further studies will be needed to establish the real role of PGE2 during hypoxia injury of the heart in the course of autoimmune diseases. 展开更多
关键词 MYOCARDIUM PGE2 HYPOXIA Histopathology Periodontitis Antibodies anti-β1 Adrenoceptors XAMOTEROL
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ELISA法和化学发光法对血清中HIV-1HIV-2抗体、梅毒抗体和丙肝抗体的检测意义 被引量:16
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作者 张巧安 《广东微量元素科学》 CAS 2016年第5期17-19,共3页
目的探讨分析ELLSA法(酶联免疫吸附法)和化学发光法对血清中HIV-1/HIV-2抗体、梅毒抗体和丙抗体的临床检测意义。方法将382份脐血作为研究分析对象,分别采用酶联免疫吸附和化学发光法对所选标本进行检测,包含丙肝抗体、梅毒抗体、HIV-1/... 目的探讨分析ELLSA法(酶联免疫吸附法)和化学发光法对血清中HIV-1/HIV-2抗体、梅毒抗体和丙抗体的临床检测意义。方法将382份脐血作为研究分析对象,分别采用酶联免疫吸附和化学发光法对所选标本进行检测,包含丙肝抗体、梅毒抗体、HIV-1/HIV-2抗体检测。结果采用ELLSA检测后HIV-1/HIV-2抗体、梅毒抗体、丙肝抗体阳性率分别为0.52%、0.79%、1.05%。采用化学发光法检测HIV-1/HIV-2抗体、梅毒抗体、丙肝抗体3种检测的阳性率分别为0.79%、1.05%、1.31%。标准抗体品梯度稀释后再进行检测,将此3种抗体稀释程度为10 pg/m L,三组抗体采用化学发光法进行检测,其结果均为阳性,采用酶联免疫吸附方式进行检测的只有梅毒抗体呈阳性,其余两项均为阴性。结论化学发光法作为临床进行血清中HIV-1/HIV-2抗体、梅毒抗体、丙肝抗体检测的首选方案,此检测方式具有更高的灵敏性。 展开更多
关键词 酶联免疫吸附法 化学发光法 血清 HIV-1/HIV-2抗体 梅毒抗体 丙肝抗体
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HIV抗原抗体光激化学发光法联合检测试剂的评价 被引量:5
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作者 宋淑静 马小亮 +4 位作者 杨晓玲 刘亚楠 陈丽娟 李娟 王雅杰 《标记免疫分析与临床》 CAS 2019年第10期1758-1761,共4页
目的评价北京科美生物技术有限公司的人类免疫缺陷病毒抗原抗体(HIV Ag/Ab)检测试剂盒(光激化学发光法)与已上市HIV抗原抗体检测试剂盒的一致性以及血清转化灵敏度。方法收集363例血清样本,包括HIV患者样本、健康人员样本、以及类风湿... 目的评价北京科美生物技术有限公司的人类免疫缺陷病毒抗原抗体(HIV Ag/Ab)检测试剂盒(光激化学发光法)与已上市HIV抗原抗体检测试剂盒的一致性以及血清转化灵敏度。方法收集363例血清样本,包括HIV患者样本、健康人员样本、以及类风湿因子样本、孕妇样本、ANA阳性样本、乙型病毒性肝炎和丙型病毒性肝炎患者等干扰样本;另外有10套HIV血清转换盘共120份血清样本。检测采用LiCA 500自动光激化学发光检测仪和相应的检测试剂、校准品和质控品以及检测参数,定性检测血清HIV1 p24抗原和HIV1/2抗体并评价与参比试剂结果一致性和血清转化灵敏度。结果北京科美生物技术有限公司的人类免疫缺陷病毒抗原抗体(HIVAg/Ab)检测试剂盒(光激化学发光法)血清样本检测结果和参比试剂检测结果具有较好一致性;评估试剂的血清转化灵敏度同参比试剂相当。结论北京科美生物技术有限公司的人类免疫缺陷病毒抗原抗体(HIVAg/Ab)检测试剂盒(光激化学发光法)与已上市同品种试剂盒检测结果具有一致性,血清转换灵敏度较高,有利于筛查HIV早期感染,为临床HIV早期诊断提供有效的实验室诊断依据。 展开更多
关键词 人类免疫缺陷病毒抗原抗体(HIV Ag/Ab)检测试剂盒(光激化学发光法) HIV p24抗原和HIV1/2抗体 结果一致性 血清转换灵敏度
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Antiphospholipid syndrome and its role in pediatric cerebrovascular diseases: A literature review 被引量:1
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作者 Beata Sarecka-Hujar Ilona Kopyta 《World Journal of Clinical Cases》 SCIE 2020年第10期1806-1817,共12页
Antiphospholipid syndrome(APS)or Hughes syndrome is an acquired thromboinflammatory disorder.Clinical criteria of APS diagnosis are large-and small-vessel thrombosis as well as obstetric problems;laboratory criteria a... Antiphospholipid syndrome(APS)or Hughes syndrome is an acquired thromboinflammatory disorder.Clinical criteria of APS diagnosis are large-and small-vessel thrombosis as well as obstetric problems;laboratory criteria are the presence of antiphospholipid antibodies(lupus anticoagulant,anticardiolipin antibodies and anti-β2-glycoprotein-1).The presence of at least 1 clinical and 1 laboratory criterion allows definitive diagnosis of APS.Primary APS is diagnosed in patients without features of connective tissue disease;secondary APS is diagnosed in patients with clinical signs of autoimmune disease.A high frequency of catastrophic APS as well as a high tendency to evolve from primary APS to secondary syndrome during the course of lupus and lupus-like disease is a feature of pediatric APS.The most characteristic clinical presentation of APS in the pediatric population is venous thrombosis,mainly in the lower limbs,and arterial thrombosis causing ischemic brain stroke.Currently,no diagnostic criteria for pediatric APS exist,which probably results in an underestimation of the problem.Similarly,no therapeutic procedures for APS specific for children have yet been established.In the present literature review,we discussed data concerning APS in children and its role in cerebrovascular diseases,including pediatric arterial ischemic stroke,migraine and cerebral venous thrombosis. 展开更多
关键词 Antiphospholipid syndrome Antiphospholipid antibodies Lupus anticoagulant anti-β2-glycoprotein-1 CHILDREN THROMBOSIS
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